Next Generation Instrument for In-Depth Analysis of Mitochondrial Fitness

Deliverables

Report containing updated communication strategy and activities performed during Year 1 of the project.

Report containing the final communication plan and all the activities and results performed during Year 1 and Year 2 of the project

Final SOPs and User’s Manual for each module

A compiled list of SOPs and protocols in the User Manual that will be posted online and accompany the NextGenO2k device

Website

Updated website with a dedicated section for NextGen-O2k Phase 2 project.

Publications

Comparable respiratory activity in attached and suspended human fibroblasts.

Author(s): Zdrazilova L, Hansikova H, Gnaiger E
Published in: MitoFit Preprints, Issue 2021.7, 2021
Publisher: Oroboros Instruments

O2k-FluoRespirometer manual

Author(s): Gnaiger E, Komlódi T, Schmitt S, Cecatto C, Gradl L, Gradl P
Published in: Mitochondrial Physiology Network, Issue 22.11(v9), 2021
Publisher: Oroboros Instruments

Hepatitis C virus-linked mitochondrial dysfunction promotes hypoxia-inducible factor 1α-mediated glycolytic adaptation

Author(s): Cardoso LHD and Tindle-Solomon L
Published in: O2k-brief, Issue 21/07/2020, 2020
Publisher: Oroboros Instruments

Experimental oxygen concentration influences rates of mitochondrial hydrogen peroxide release from cardiac and skeletal muscle preparations

Author(s): Komlodi T and Tindle-Solomon L
Published in: O2k-brief, Issue 24/06/2020, 2020
Publisher: Oroboros Instruments

Mitochondrial dysfunction in peripheral blood mononuclear cells in early experimental and clinical acute pancreatitis. Pancreatology

Author(s): Falaye T, Sobotka O and Tindle-Solomon L
Published in: O2k-brief, Issue 30/07/2020, 2020
Publisher: Oroboros Instruments

NextGen-O2k: PB-Module manual

Author(s): Di Marcello M, Merth A, Doerrier C, Schmitt S, Schwaninger H, Walter-Vracevic M, Gradl P, Gnaiger E
Published in: Mitochondrial Physiology Network, Issue 26.11, 2021
Publisher: Oroboros Instruments

O2k Quality Control 1: Polarographic oxygen sensors and accuracy of calibration - DatLab 8.

Author(s): Gnaiger E
Published in: Mitochondrial Physiology Network, Issue 06.03, 2021
Publisher: Oroboros Instruments

Service of the polarographic oxygen sensor OroboPOS.

Author(s): Gnaiger E
Published in: Mitochondrial Physiology Network, Issue 19.18B(v9), 2020
Publisher: Oroboros Instruments

DatLab-guide through the menus

Author(s): Gnaiger E, Cecatto C, Gradl L
Published in: Mitochondrial Physiology Network, Issue 26.06, 2021
Publisher: Oroboros Instruments

O2k-Chamber cleaning SOP and Integrated Suction System (ISS).

Author(s): Di Marcello M, Komlodi T, Schmitt S, Gnaiger E
Published in: Mitochondrial Physiology Network, Issue 19.03(v7), 2021
Publisher: Oroboros Instruments

O2k-MultiSensor system with ion selective electrodes (ISE).

Author(s): Komlodi T, Schmitt S, Gnaiger E
Published in: Mitochondrial Physiology Network, Issue 15.03(v9), 2021
Publisher: Oroboros Instruments

Cell-permeable succinate rescues mitochondrial respiration in cellular models of statin toxicity

Author(s): Cecatto C and Tindle-Solomon L
Published in: O2k-brief, Issue 27/04/2021, 2021
Publisher: Oroboros Instruments

O2k-checklist: get started with an O2k-experiment

Author(s): Doerrier C, Schmitt S, Cecatto C, Gnaiger E
Published in: Mitochondrial Physiology Network, Issue 20.04(v5), 2021
Publisher: Oroboros Instruments

Bioenergetic cluster analysis – mitochondrial respiratory control in human fibroblasts

Author(s): Gnaiger E
Published in: MitoFit Preprints, Issue 2021.8, 2021
Publisher: Oroboros Instruments

Doxorubicin-transferrin conjugate alters mitochondrial homeostasis and energy metabolism in human breast cancer cells

Author(s): Komlodi T and Tindle-Solomon L
Published in: O2k-brief, Issue 30/06/2021, 2021
Publisher: Oroboros Instruments

Magnesium Green for fluorometric measurement of ATP production does not interfere with mitochondrial respiration

Author(s): Cardoso LHD, Doerrier C, Gnaiger
Published in: Bioenergetics Communications, Issue 2021.1, 2020
Publisher: Oroboros Instruments

Oncogenic HSP60 regulates mitochondrial oxidative phosphorylation to support Erk1/2 activation during pancreatic cancer cell growth

Author(s): Cecatto C and Tindle-Solomon L
Published in: O2k-brief, Issue 18/11/2020, 2020
Publisher: Oroboros Instruments

Laboratory protocol: isolation of mouse heart mitochondria

Author(s): Komlodi T, Cardoso LHD, Gnaiger E
Published in: Mitochondrial Physiology Network, Issue 20.068v2), 2021
Publisher: Oroboros Instruments

High-resolution respirometry for chloroplast and mitochondrial bioenergetics in Chlamydomonas reinhardtii ― towards biotechnology exploitations

Author(s): Huete-Ortega M, Di Marcello M, Iglesias-Gonzalez J, Gnaiger E
Published in: MitoFit Preprints, 2020
Publisher: Oroboros Instruments

Magnesium Green for fluorometric measurement of ATP production does not interfere with mitochondrial respiration.

Author(s): Cardoso LHD, Doerrier C, Gnaiger E
Published in: MitoFit Preprints, Issue 2021.1, 2021
Publisher: Oroboros Instruments

Effects of ultramarathon running on mitochondrial function of platelets and oxidative stress parameters: a pilot study

Author(s): Cecatto C and Tindle-Solomon L
Published in: O2k-brief, Issue 16/09/2021, 2021
Publisher: Oroboros Instruments

O2k-Specifications for respirometry and comprehensive OXPHOS analysis

Author(s): Gnaiger E
Published in: Mitochondrial Physiology Network, Issue 18.10(09), 2019, Page(s) 1-8
Publisher: Oroboros Instruments

O2k Quality Control 2: Instrumental oxygen background correction and accuracy of oxygen flux

Author(s): Fasching M, Gnaiger E
Published in: Mitochondrial Physiology Network, Issue 14.06(08), 2020, Page(s) 1-16
Publisher: Oroboros Instruments

NextGen-O2k: Q-Module Manual

Author(s): Komlodi T, Cardoso LHD, Gollner M, Merth A, Hunger M, Iglesias-Gonzalez J, Doerrier C, Tindle-Solomon L, Schwaninger H, Walter-Vracevic M, Gradl P, Moore AL, Gnaiger E
Published in: Mitochondrial Physiology Network, Issue 24.12(02), 2020, Page(s) 1-21
Publisher: Oroboros Instruments

DatLab 8.0: Cyclic voltammetry manual

Author(s): Komlodi T, Cardoso LHD, Haider M, Tindle-Solomon L, Iglesias-Gonzalez J, Gnaiger E
Published in: Mitochondrial Physiology Network, Issue 24.16(01), 2019, Page(s) 1-10
Publisher: Oroboros Instruments

Oxygen flux analysis with DatLab 7.4

Author(s): Cardoso LHD, Antunes D, Iglesias-Gonzalez J, Komlodi T, Doerrier C, Garcia-Souza LF, Gnaiger E, Sobotka O
Published in: Mitochondrial Physiology Network, Issue 24.06(02), 2020, Page(s) 1-11
Publisher: Oroboros Instruments

Hydrogen peroxide flux analysis using Amplex Ultra Red assay in MiR05-Kit with DatLab 7.4

Author(s): Komlodi T, Iglesias-Gonzalez J, Cardoso LHD, Sobotka O, Gnaiger E
Published in: Mitochondrial Physiology Network, Issue 24.10(02), 2020, Page(s) 1-6
Publisher: Oroboros Instruments

Data analysis of mitochondrial membrane potential estimation using safranin

Author(s): Komlodi T, Fasching M, Antunes D, Iglesias-Gonzalez J, Sobotka O, Gnaiger E
Published in: Mitochondrial Physiology Network, Issue 24.08(02), 2020, Page(s) 1-8
Publisher: Oroboros Instruments

Data analysis of mitochondrial membrane potential estimation using various fluorescence dyes

Author(s): Komlodi T, Antunes D, Iglesias-Gonzalez J, Sobotka O, Gnaiger E
Published in: Mitochondrial Physiology Network, Issue 24.09(02), 2020, Page(s) 1-5
Publisher: Oroboros Instruments

Mitochondrial respiratory states and rates

Author(s): Gnaiger E, Aasander Frostner E, Abdul Karim N, Abdel-Rahman EA, Abumrad NA, Acuna-Castroviejo D, Adiele RC, Ahn B, Alencar MB, Ali SS, Almeida A, Alton L, Alve sMG, Amati F, Amoedo ND, Amorim R, Andreadou I, Antunes D, Aragó M, Aral C, Arandarčikaitė O,Arias Reyes C, Armand AS, Arnould T, Avram VF, Bailey DM, Bairam A, Bajpeyi S, Bajzikova M, Bakker BM, Banni A, Bardal T, Barlow J, Bastos Sant'
Published in: MitoFit Preprint Archives, 2019
Publisher: Oroboros Instruments

Mitochondrial physiology

Author(s): Gnaiger E, Aasander Frostner E, Abdul Karim N, Abdel-Rahman EA, Abumrad NA, Acuna-Castroviejo D, Adiele RC, Ahn B, Alencar MB, Ali SS, Almeida A, Alton L, Alve sMG, Amati F, Amoedo ND, Amorim R, Andreadou I, Antunes D, Aragó M, Aral C, Arandarčikaitė O,Arias Reyes C, Armand AS, Arnould T, Avram VF, Bailey DM, Bairam A, Bajpeyi S, Bajzikova M, Bakker BM, Banni A, Bardal T, Barlow J, Bastos Sant'
Published in: Bioenergetics Communications, 2020
Publisher: Oroboros Instruments

Inter-laboratory harmonization of respiratory protocols in permeabilized human muscle fibers

Author(s): Doerrier C, Gama-Perez P, Distefano G, Pesta D, Soendergaard SD, Chroeis KM, Gonzalez-Franquesa A, Goodpaster BH, Coen P, Larsen S, Gnaiger E, Garcia-Roves PM
Published in: MitoFit Preprint Archives, 2019
Publisher: Oroboros Instruments

Partitioning between cytochrome c oxidase and alternative oxidase studied by oxygen kinetics of dark respiration in Chlamydomonas reinhardtii: a microalgae model organism

Author(s): Di Marcello M, Iglesias-Gonzalez J, Meszaros A, Haider M, Gnaiger E, Huete-Ortega M
Published in: MitoFit Preprint Archives, 2019
Publisher: Oroboros Instruments

Generating reference values on mitochondrial respiration in permeabilized muscle fibers

Author(s): Chabi Béatrice, Ost M, Gama-Perez P, Dahdah N, Lemieux H, Holody CD, Carpenter RG, Tepp K, Puurand M, Kaambre T, Dubouchaud H, Cortade F, Pesta D, Calabria E, Casado M, Fernandez-Ortiz M, Acuña-Castroviejo D, Villena JA, Grefte S, Keijer J, O'Brien K, Sowton A, Murray AJ, Campbell MD, Marcinek DJ, Nollet E, Wüst R, Dayanidhi S, Gnaiger E, Doerrier C, Garcia-Roves PM
Published in: MitoFit Preprint Archives, 2019
Publisher: Oroboros Instruments

Commitment to reproducibility in targeting mitochondrial respiratory control: basic and advanced applications of the O2k-FluoRespirometer

Author(s): Doerrier C, Di Marcello M, Gnaiger E
Published in: 2020
Publisher: Foundation for Translational Myology

Endogenous quinones sustain a moderate NADH oxidation by Complex I during anoxia

Author(s): Ravasz D, Bui D, Kitayev A, Greenwood B, Hill C, Komlodi T, Doerrier C, Ozohanics O, Moore AL, Gnaiger E, Kiebish M, Kolev K, Seyfried TN, Willis WT, Narain N, Adam-Vizi V, Chinopoulos C
Published in: 2019
Publisher: Mitochondrial Physiology Network

Succinate dehydrogenase is a source of reactive oxygen species in plants and regulates development and stress responses

Author(s): Huete-Ortega M
Published in: O2k-brief, Issue 2019-06-05, 2019
Publisher: Oroboros Instruments

Regulation of ATP production: dependence on calcium concentration and respiratory state

Author(s): Cardoso L
Published in: O2k-brief, Issue 2019-06-12, 2019
Publisher: Oroboros Instruments

Mitochondrial respiration in peripheral blood mono nuclear cells correlates with depressive sub-symptoms and severity of major depression

Author(s): Garcia-Souza LF
Published in: O2k-brief, Issue 2019-06-19, 2019
Publisher: Oroboros Instruments

Mitochondrial respiratory control and early defects of oxidative phosphorylation in the failing human heart

Author(s): Gnaiger E and Iglesias-Gonzalez J
Published in: O2k-brief, Issue 2019-06-26, 2019
Publisher: Oroboros Instruments

High intensity exercise inhibits carnitine palmoyltranferase-I sensitivity to L-carnitine

Author(s): Krumschnable G
Published in: O2k-brief, Issue 2019-07-02, 2019
Publisher: Oroboros Instruments

Rapid kinetics of changes in oxygen consumption rate in thrombin-stimulated platelets measured by high-resolution respirometry

Author(s): Garcia-Souza LF
Published in: O2k-brief, Issue 2019-07-09, 2019
Publisher: Oroboros Instruments

Direct effects of phenformin on metabolism/bioenergetics and viability of SH-SY5Y neuroblastoma cells

Author(s): Bastos AC
Published in: O2k-brief, 2019
Publisher: Oroboros Instruments

Selective disruption of respiratory supercomplexes as a new strategy to supress Her2high breast cancer

Author(s): Bastos AC
Published in: O2k-brief, Issue 2019-07-24, 2019
Publisher: Oroboros Instruments

Endogenous myoglobin in breast cancer is hypoxia-inducible by alternate transcription and functions to impair mitochondrial activity: a role in tumor suppression?

Author(s): Iglesias-Gonzalez J
Published in: O2k-brief, Issue 2019-07-31, 2019
Publisher: Oroboros Instruments

6-Hydroxidopamine induces different mitochondrial bioenergetics response in brain regions of rat

Author(s): Iglesias-Gonzalez J
Published in: O2k-brief, Issue 2019-08-07, 2019
Publisher: Oroboros Instruments

Non-canonical BAD activity regulates breast cancer cells and tumor growth via 14-3-3 binding and mitochondrial metabolism

Author(s): Tindle-Solomon L
Published in: O2k-brief, Issue 2019-10-25, 2019
Publisher: Oroboros Instruments

Measurement of ADP-ATP exchange in relation to mitochondrial transmembrane potential and oxygen consumption

Author(s): Tindle-Solomon L
Published in: O2k-brief, Issue 2019-11-28, 2019
Publisher: Oroboros Instruments

Analysis of respiratory capacity in brain tissue preparations: high-resolution respirometry for intact hippocampal slices

Author(s): Tindle-Solomon L
Published in: O2k-brief, Issue 2019-11-12, 2019
Publisher: Oroboros Instruments

Inhibition of mitochondrial respiration prevents BRAF-mutant melanoma brain metastasis

Author(s): Antunes D and Tindle-Solomon L
Published in: O2k-brief, Issue 2020-01-20, 2020
Publisher: Oroboros Instruments

The metabolite BH4 controls T cell proliferation in autoimmunity and cancer

Author(s): Garcia-Souza LF and Tindle-Solomon L
Published in: O2k-brief, Issue 2020-02-04, 2020
Publisher: Oroboros Instruments

Effects of aging and exercise training on mitochondrial function and apoptosis in the rat heart

Author(s): Cecatto C and Tindle-Solomon L
Published in: O2k-brief, Issue 2020-03-18, 2020
Publisher: Oroboros Instruments

Effect of initial aging and high-fat/high-fructose diet on mitochondrial bioenergetics and oxidative status in rat brain

Author(s): Antunes D and Tindle-Solomon L
Published in: O2k-brief, Issue 2020-03-05, 2020
Publisher: Oroboros Instruments

Muscle-derived GDF15 drives diurnal anorexia and systemic metabolic remodeling during mitochondrial stress

Author(s): Antunes D and Tindle-Solomon L
Published in: O2k-brief, Issue 2020-04-03, 2020
Publisher: Oroboros Instruments

Respiratory chain signaling is essential for adaptive remodeling following cardiac ischaemia

Author(s): Komlodi T and Tindle-Solomon L
Published in: O2k-brief, Issue 2020-05-15, 2020
Publisher: Oroboros Instruments

OXPHOS remodeling n high-grade prostate cancer involves mtDNA mutations and increased succinate oxidation

Author(s): Bastos AC and Tindle-Solomon L
Published in: O2k-brief, Issue 2020-05-28, 2020
Publisher: Oroboros Instruments

Peripheral mitochondrial dysfunction in Alzheimer’s disease: focus on lymphocytes.

Author(s): Garcia-Souza LF and Tindle-Solomon L
Published in: O2k-brief, Issue 2020-04-16, 2020
Publisher: Oroboros Instruments

High-Fat diet activates liver iPLA2γ generating eicosanoids that mediate metabolic stress

Author(s): Komlodi T and Tindle-Solomon L
Published in: O2k-brief, Issue 15/06/2021, 2021
Publisher: Oroboros Instruments

Platelet mitochondrial respiration, endogenous coenzyme Q10 and oxidative stress in patients with chronic kidney disease

Author(s): Falaye T, Sobotka O and Tindle-Solomon L
Published in: O2k-brief, Issue 29/05/2020, 2020
Publisher: Oroboros Instruments

NextGen-O2k manual

Author(s): Gnaiger E, Cardoso LHD, Cecatto C, Niedenzu W, Haider M, Gollner M, Merth A, Schwaninger H, Walter-Vracevic M, Gradl P
Published in: Mitochondrial Physiology Network, Issue 26.13, 2021
Publisher: Oroboros Instruments

Measurement of mitochondrial ATP production using Magnesium Green: DL-Protocols and data analysis with DatLab 7.4

Author(s): Cardoso LHD, Komlodi T, Gnaiger E
Published in: Mitochondrial Physiology Network, Issue 26.10, 2021
Publisher: Oroboros Instruments

NextGen-O2k: NADH-Module manual

Author(s): Doerrier C, Schwaninger H, Walter-Vracevic M, Haider M, Niedenzu W, Komlódi T, Gradl P, Gnaiger E
Published in: Mitochondrial Physiology Network, Issue 26.12, 2021
Publisher: Oroboros Instruments

Hippocampal growth factor and myokine cathepsin B expression following aerobic and resistance training in 3xTg-AD mice

Author(s): Cecatto C and Tindle-Solomon L
Published in: O2k-brief, Issue 18/09/2020, 2020
Publisher: Oroboros Instruments

Adaptation of mitochondrial substrate flux in a mouse model of nonalcoholic fatty liver disease

Author(s): Komlodi T and Tindle-Solomon L
Published in: O2k-brief, Issue 04/01/2021, 2021
Publisher: Oroboros Instruments

An mtDNA mutant mouse demonstrates that mitochondrial deficiency can result in autism endophenotypes

Author(s): Komlodi T and Tindle-Solomon L
Published in: O2k-brief, Issue 29/03/2021, 2021
Publisher: Oroboros Instruments

Ex vivo mitochondrial respiration parallels biochemical response to ibrutinib in CLL cells

Author(s): Komlodi T and Tindle-Solomon L
Published in: O2k-brief, Issue 16/08/2021, 2021
Publisher: Oroboros Instruments

Oxygen dependence of photosynthesis and light-enhanced dark respiration studied by High-Resolution PhotoRespirometry

Author(s): Went N, Di Marcello M, Gnaiger E
Published in: MitoFit Preprints, Issue 2021.5, 2021
Publisher: Oroboros Instruments

The elementary unit — canonical reviewer's comments on: Bureau International des Poids et Mesures

Author(s): Gnaiger E
Published in: MitoFit Preprints, Issue 2021.4(v2), 2021
Publisher: Oroboros Instruments

Colon cancer cell differentiation by sodium butyrate modulates metabolic plasticity of Caco-2 cells via alteration of phosphotransfer network

Author(s): Komlodi T and Tindle-Solomon L
Published in: O2k-brief, Issue 09/06/2021, 2019
Publisher: Oroboros Instruments

Titration-Injection microPump TIP2k manual.

Author(s): Gradl L, Gradl P, Gnaiger E
Published in: Mitochondrial Physiology Network, Issue 12.10(v9), 2021
Publisher: Oroboros Instruments

Coupling and pathway control of coenzyme Q redox state and respiration in isolated mitochondria

Author(s): Komlódi T, Cardoso LHD, Doerrier C, Moore AL, Rich PR, Gnaiger E
Published in: MitoFit Preprints, Issue 2021.2(v4), 2021
Publisher: Oroboros Instruments

Mitochondrial respiration of platelets: comparison of isolation methods.

Author(s): Vernerova A, Garcia-Souza LF, Soucek O, Kostal M, Rehacek V, Krcmova LK, Gnaiger E, Sobotka O
Published in: MitoFit Preprints, Issue 2021.6, 2021
Publisher: Oroboros Instruments

Mitochondrial pathways and respiratory control

Author(s): Gnaiger E
Published in: Bioenergetics Communicatoins, Issue 2020.2, 2020, ISBN 978-3-9504814-2-6
Publisher: Bioenergetics Communicatoins

Intellectual Property Rights

High-Resolution Respirometry (HRR)

Application/Publication number: EU 018170785
Date: 2020-05-25
Applicant(s): OROBOROS INSTRUMENTS GmbH

DatLab

Application/Publication number: EU 018170784
Date: 2020-05-25
Applicant(s): OROBOROS INSTRUMENTS GmbH

NextGen-O2k

Application/Publication number: EU 018170776
Date: 2020-05-25
Applicant(s): OROBOROS INSTRUMENTS GmbH

O2k

Application/Publication number: EU 018480189
Date: 2021-05-27
Applicant(s): OROBOROS INSTRUMENTS GmbH

Deliverables

Publications

Intellectual Property Rights

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