Deliverables Documents, reports (3) Intermediate Communication Plan Report containing updated communication strategy and activities performed during Year 1 of the project. Final Communication Plan Report containing the final communication plan and all the activities and results performed during Year 1 and Year 2 of the project Final SOPs and User’s Manual for each module A compiled list of SOPs and protocols in the User Manual that will be posted online and accompany the NextGenO2k device Demonstrators, pilots, prototypes (1) Website Updated website with a dedicated section for NextGen-O2k Phase 2 project. Publications Other (72) Comparable respiratory activity in attached and suspended human fibroblasts. Author(s): Zdrazilova L, Hansikova H, Gnaiger E Published in: MitoFit Preprints, Issue 2021.7, 2021 Publisher: Oroboros Instruments O2k-FluoRespirometer manual Author(s): Gnaiger E, Komlódi T, Schmitt S, Cecatto C, Gradl L, Gradl P Published in: Mitochondrial Physiology Network, Issue 22.11(v9), 2021 Publisher: Oroboros Instruments Hepatitis C virus-linked mitochondrial dysfunction promotes hypoxia-inducible factor 1α-mediated glycolytic adaptation Author(s): Cardoso LHD and Tindle-Solomon L Published in: O2k-brief, Issue 21/07/2020, 2020 Publisher: Oroboros Instruments Experimental oxygen concentration influences rates of mitochondrial hydrogen peroxide release from cardiac and skeletal muscle preparations Author(s): Komlodi T and Tindle-Solomon L Published in: O2k-brief, Issue 24/06/2020, 2020 Publisher: Oroboros Instruments Mitochondrial dysfunction in peripheral blood mononuclear cells in early experimental and clinical acute pancreatitis. Pancreatology Author(s): Falaye T, Sobotka O and Tindle-Solomon L Published in: O2k-brief, Issue 30/07/2020, 2020 Publisher: Oroboros Instruments NextGen-O2k: PB-Module manual Author(s): Di Marcello M, Merth A, Doerrier C, Schmitt S, Schwaninger H, Walter-Vracevic M, Gradl P, Gnaiger E Published in: Mitochondrial Physiology Network, Issue 26.11, 2021 Publisher: Oroboros Instruments O2k Quality Control 1: Polarographic oxygen sensors and accuracy of calibration - DatLab 8. Author(s): Gnaiger E Published in: Mitochondrial Physiology Network, Issue 06.03, 2021 Publisher: Oroboros Instruments Service of the polarographic oxygen sensor OroboPOS. Author(s): Gnaiger E Published in: Mitochondrial Physiology Network, Issue 19.18B(v9), 2020 Publisher: Oroboros Instruments DatLab-guide through the menus Author(s): Gnaiger E, Cecatto C, Gradl L Published in: Mitochondrial Physiology Network, Issue 26.06, 2021 Publisher: Oroboros Instruments O2k-Chamber cleaning SOP and Integrated Suction System (ISS). Author(s): Di Marcello M, Komlodi T, Schmitt S, Gnaiger E Published in: Mitochondrial Physiology Network, Issue 19.03(v7), 2021 Publisher: Oroboros Instruments O2k-MultiSensor system with ion selective electrodes (ISE). Author(s): Komlodi T, Schmitt S, Gnaiger E Published in: Mitochondrial Physiology Network, Issue 15.03(v9), 2021 Publisher: Oroboros Instruments Cell-permeable succinate rescues mitochondrial respiration in cellular models of statin toxicity Author(s): Cecatto C and Tindle-Solomon L Published in: O2k-brief, Issue 27/04/2021, 2021 Publisher: Oroboros Instruments O2k-checklist: get started with an O2k-experiment Author(s): Doerrier C, Schmitt S, Cecatto C, Gnaiger E Published in: Mitochondrial Physiology Network, Issue 20.04(v5), 2021 Publisher: Oroboros Instruments Bioenergetic cluster analysis – mitochondrial respiratory control in human fibroblasts Author(s): Gnaiger E Published in: MitoFit Preprints, Issue 2021.8, 2021 Publisher: Oroboros Instruments Doxorubicin-transferrin conjugate alters mitochondrial homeostasis and energy metabolism in human breast cancer cells Author(s): Komlodi T and Tindle-Solomon L Published in: O2k-brief, Issue 30/06/2021, 2021 Publisher: Oroboros Instruments Magnesium Green for fluorometric measurement of ATP production does not interfere with mitochondrial respiration Author(s): Cardoso LHD, Doerrier C, Gnaiger Published in: Bioenergetics Communications, Issue 2021.1, 2020 Publisher: Oroboros Instruments Oncogenic HSP60 regulates mitochondrial oxidative phosphorylation to support Erk1/2 activation during pancreatic cancer cell growth Author(s): Cecatto C and Tindle-Solomon L Published in: O2k-brief, Issue 18/11/2020, 2020 Publisher: Oroboros Instruments Laboratory protocol: isolation of mouse heart mitochondria Author(s): Komlodi T, Cardoso LHD, Gnaiger E Published in: Mitochondrial Physiology Network, Issue 20.068v2), 2021 Publisher: Oroboros Instruments High-resolution respirometry for chloroplast and mitochondrial bioenergetics in Chlamydomonas reinhardtii ― towards biotechnology exploitations Author(s): Huete-Ortega M, Di Marcello M, Iglesias-Gonzalez J, Gnaiger E Published in: MitoFit Preprints, 2020 Publisher: Oroboros Instruments Magnesium Green for fluorometric measurement of ATP production does not interfere with mitochondrial respiration. Author(s): Cardoso LHD, Doerrier C, Gnaiger E Published in: MitoFit Preprints, Issue 2021.1, 2021 Publisher: Oroboros Instruments Effects of ultramarathon running on mitochondrial function of platelets and oxidative stress parameters: a pilot study Author(s): Cecatto C and Tindle-Solomon L Published in: O2k-brief, Issue 16/09/2021, 2021 Publisher: Oroboros Instruments O2k-Specifications for respirometry and comprehensive OXPHOS analysis Author(s): Gnaiger E Published in: Mitochondrial Physiology Network, Issue 18.10(09), 2019, Page(s) 1-8 Publisher: Oroboros Instruments O2k Quality Control 2: Instrumental oxygen background correction and accuracy of oxygen flux Author(s): Fasching M, Gnaiger E Published in: Mitochondrial Physiology Network, Issue 14.06(08), 2020, Page(s) 1-16 Publisher: Oroboros Instruments NextGen-O2k: Q-Module Manual Author(s): Komlodi T, Cardoso LHD, Gollner M, Merth A, Hunger M, Iglesias-Gonzalez J, Doerrier C, Tindle-Solomon L, Schwaninger H, Walter-Vracevic M, Gradl P, Moore AL, Gnaiger E Published in: Mitochondrial Physiology Network, Issue 24.12(02), 2020, Page(s) 1-21 Publisher: Oroboros Instruments DatLab 8.0: Cyclic voltammetry manual Author(s): Komlodi T, Cardoso LHD, Haider M, Tindle-Solomon L, Iglesias-Gonzalez J, Gnaiger E Published in: Mitochondrial Physiology Network, Issue 24.16(01), 2019, Page(s) 1-10 Publisher: Oroboros Instruments Oxygen flux analysis with DatLab 7.4 Author(s): Cardoso LHD, Antunes D, Iglesias-Gonzalez J, Komlodi T, Doerrier C, Garcia-Souza LF, Gnaiger E, Sobotka O Published in: Mitochondrial Physiology Network, Issue 24.06(02), 2020, Page(s) 1-11 Publisher: Oroboros Instruments Hydrogen peroxide flux analysis using Amplex Ultra Red assay in MiR05-Kit with DatLab 7.4 Author(s): Komlodi T, Iglesias-Gonzalez J, Cardoso LHD, Sobotka O, Gnaiger E Published in: Mitochondrial Physiology Network, Issue 24.10(02), 2020, Page(s) 1-6 Publisher: Oroboros Instruments Data analysis of mitochondrial membrane potential estimation using safranin Author(s): Komlodi T, Fasching M, Antunes D, Iglesias-Gonzalez J, Sobotka O, Gnaiger E Published in: Mitochondrial Physiology Network, Issue 24.08(02), 2020, Page(s) 1-8 Publisher: Oroboros Instruments Data analysis of mitochondrial membrane potential estimation using various fluorescence dyes Author(s): Komlodi T, Antunes D, Iglesias-Gonzalez J, Sobotka O, Gnaiger E Published in: Mitochondrial Physiology Network, Issue 24.09(02), 2020, Page(s) 1-5 Publisher: Oroboros Instruments Mitochondrial respiratory states and rates Author(s): Gnaiger E, Aasander Frostner E, Abdul Karim N, Abdel-Rahman EA, Abumrad NA, Acuna-Castroviejo D, Adiele RC, Ahn B, Alencar MB, Ali SS, Almeida A, Alton L, Alve sMG, Amati F, Amoedo ND, Amorim R, Andreadou I, Antunes D, Aragó M, Aral C, Arandarčikaitė O,Arias Reyes C, Armand AS, Arnould T, Avram VF, Bailey DM, Bairam A, Bajpeyi S, Bajzikova M, Bakker BM, Banni A, Bardal T, Barlow J, Bastos Sant' Published in: MitoFit Preprint Archives, 2019 Publisher: Oroboros Instruments Mitochondrial physiology Author(s): Gnaiger E, Aasander Frostner E, Abdul Karim N, Abdel-Rahman EA, Abumrad NA, Acuna-Castroviejo D, Adiele RC, Ahn B, Alencar MB, Ali SS, Almeida A, Alton L, Alve sMG, Amati F, Amoedo ND, Amorim R, Andreadou I, Antunes D, Aragó M, Aral C, Arandarčikaitė O,Arias Reyes C, Armand AS, Arnould T, Avram VF, Bailey DM, Bairam A, Bajpeyi S, Bajzikova M, Bakker BM, Banni A, Bardal T, Barlow J, Bastos Sant' Published in: Bioenergetics Communications, 2020 Publisher: Oroboros Instruments Inter-laboratory harmonization of respiratory protocols in permeabilized human muscle fibers Author(s): Doerrier C, Gama-Perez P, Distefano G, Pesta D, Soendergaard SD, Chroeis KM, Gonzalez-Franquesa A, Goodpaster BH, Coen P, Larsen S, Gnaiger E, Garcia-Roves PM Published in: MitoFit Preprint Archives, 2019 Publisher: Oroboros Instruments Partitioning between cytochrome c oxidase and alternative oxidase studied by oxygen kinetics of dark respiration in Chlamydomonas reinhardtii: a microalgae model organism Author(s): Di Marcello M, Iglesias-Gonzalez J, Meszaros A, Haider M, Gnaiger E, Huete-Ortega M Published in: MitoFit Preprint Archives, 2019 Publisher: Oroboros Instruments Generating reference values on mitochondrial respiration in permeabilized muscle fibers Author(s): Chabi Béatrice, Ost M, Gama-Perez P, Dahdah N, Lemieux H, Holody CD, Carpenter RG, Tepp K, Puurand M, Kaambre T, Dubouchaud H, Cortade F, Pesta D, Calabria E, Casado M, Fernandez-Ortiz M, Acuña-Castroviejo D, Villena JA, Grefte S, Keijer J, O'Brien K, Sowton A, Murray AJ, Campbell MD, Marcinek DJ, Nollet E, Wüst R, Dayanidhi S, Gnaiger E, Doerrier C, Garcia-Roves PM Published in: MitoFit Preprint Archives, 2019 Publisher: Oroboros Instruments Commitment to reproducibility in targeting mitochondrial respiratory control: basic and advanced applications of the O2k-FluoRespirometer Author(s): Doerrier C, Di Marcello M, Gnaiger E Published in: 2020 Publisher: Foundation for Translational Myology Endogenous quinones sustain a moderate NADH oxidation by Complex I during anoxia Author(s): Ravasz D, Bui D, Kitayev A, Greenwood B, Hill C, Komlodi T, Doerrier C, Ozohanics O, Moore AL, Gnaiger E, Kiebish M, Kolev K, Seyfried TN, Willis WT, Narain N, Adam-Vizi V, Chinopoulos C Published in: 2019 Publisher: Mitochondrial Physiology Network Succinate dehydrogenase is a source of reactive oxygen species in plants and regulates development and stress responses Author(s): Huete-Ortega M Published in: O2k-brief, Issue 2019-06-05, 2019 Publisher: Oroboros Instruments Regulation of ATP production: dependence on calcium concentration and respiratory state Author(s): Cardoso L Published in: O2k-brief, Issue 2019-06-12, 2019 Publisher: Oroboros Instruments Mitochondrial respiration in peripheral blood mono nuclear cells correlates with depressive sub-symptoms and severity of major depression Author(s): Garcia-Souza LF Published in: O2k-brief, Issue 2019-06-19, 2019 Publisher: Oroboros Instruments Mitochondrial respiratory control and early defects of oxidative phosphorylation in the failing human heart Author(s): Gnaiger E and Iglesias-Gonzalez J Published in: O2k-brief, Issue 2019-06-26, 2019 Publisher: Oroboros Instruments High intensity exercise inhibits carnitine palmoyltranferase-I sensitivity to L-carnitine Author(s): Krumschnable G Published in: O2k-brief, Issue 2019-07-02, 2019 Publisher: Oroboros Instruments Rapid kinetics of changes in oxygen consumption rate in thrombin-stimulated platelets measured by high-resolution respirometry Author(s): Garcia-Souza LF Published in: O2k-brief, Issue 2019-07-09, 2019 Publisher: Oroboros Instruments Direct effects of phenformin on metabolism/bioenergetics and viability of SH-SY5Y neuroblastoma cells Author(s): Bastos AC Published in: O2k-brief, 2019 Publisher: Oroboros Instruments Selective disruption of respiratory supercomplexes as a new strategy to supress Her2high breast cancer Author(s): Bastos AC Published in: O2k-brief, Issue 2019-07-24, 2019 Publisher: Oroboros Instruments Endogenous myoglobin in breast cancer is hypoxia-inducible by alternate transcription and functions to impair mitochondrial activity: a role in tumor suppression? Author(s): Iglesias-Gonzalez J Published in: O2k-brief, Issue 2019-07-31, 2019 Publisher: Oroboros Instruments 6-Hydroxidopamine induces different mitochondrial bioenergetics response in brain regions of rat Author(s): Iglesias-Gonzalez J Published in: O2k-brief, Issue 2019-08-07, 2019 Publisher: Oroboros Instruments Non-canonical BAD activity regulates breast cancer cells and tumor growth via 14-3-3 binding and mitochondrial metabolism Author(s): Tindle-Solomon L Published in: O2k-brief, Issue 2019-10-25, 2019 Publisher: Oroboros Instruments Measurement of ADP-ATP exchange in relation to mitochondrial transmembrane potential and oxygen consumption Author(s): Tindle-Solomon L Published in: O2k-brief, Issue 2019-11-28, 2019 Publisher: Oroboros Instruments Analysis of respiratory capacity in brain tissue preparations: high-resolution respirometry for intact hippocampal slices Author(s): Tindle-Solomon L Published in: O2k-brief, Issue 2019-11-12, 2019 Publisher: Oroboros Instruments Inhibition of mitochondrial respiration prevents BRAF-mutant melanoma brain metastasis Author(s): Antunes D and Tindle-Solomon L Published in: O2k-brief, Issue 2020-01-20, 2020 Publisher: Oroboros Instruments The metabolite BH4 controls T cell proliferation in autoimmunity and cancer Author(s): Garcia-Souza LF and Tindle-Solomon L Published in: O2k-brief, Issue 2020-02-04, 2020 Publisher: Oroboros Instruments Effects of aging and exercise training on mitochondrial function and apoptosis in the rat heart Author(s): Cecatto C and Tindle-Solomon L Published in: O2k-brief, Issue 2020-03-18, 2020 Publisher: Oroboros Instruments Effect of initial aging and high-fat/high-fructose diet on mitochondrial bioenergetics and oxidative status in rat brain Author(s): Antunes D and Tindle-Solomon L Published in: O2k-brief, Issue 2020-03-05, 2020 Publisher: Oroboros Instruments Muscle-derived GDF15 drives diurnal anorexia and systemic metabolic remodeling during mitochondrial stress Author(s): Antunes D and Tindle-Solomon L Published in: O2k-brief, Issue 2020-04-03, 2020 Publisher: Oroboros Instruments Respiratory chain signaling is essential for adaptive remodeling following cardiac ischaemia Author(s): Komlodi T and Tindle-Solomon L Published in: O2k-brief, Issue 2020-05-15, 2020 Publisher: Oroboros Instruments OXPHOS remodeling n high-grade prostate cancer involves mtDNA mutations and increased succinate oxidation Author(s): Bastos AC and Tindle-Solomon L Published in: O2k-brief, Issue 2020-05-28, 2020 Publisher: Oroboros Instruments Peripheral mitochondrial dysfunction in Alzheimer’s disease: focus on lymphocytes. Author(s): Garcia-Souza LF and Tindle-Solomon L Published in: O2k-brief, Issue 2020-04-16, 2020 Publisher: Oroboros Instruments High-Fat diet activates liver iPLA2γ generating eicosanoids that mediate metabolic stress Author(s): Komlodi T and Tindle-Solomon L Published in: O2k-brief, Issue 15/06/2021, 2021 Publisher: Oroboros Instruments Platelet mitochondrial respiration, endogenous coenzyme Q10 and oxidative stress in patients with chronic kidney disease Author(s): Falaye T, Sobotka O and Tindle-Solomon L Published in: O2k-brief, Issue 29/05/2020, 2020 Publisher: Oroboros Instruments NextGen-O2k manual Author(s): Gnaiger E, Cardoso LHD, Cecatto C, Niedenzu W, Haider M, Gollner M, Merth A, Schwaninger H, Walter-Vracevic M, Gradl P Published in: Mitochondrial Physiology Network, Issue 26.13, 2021 Publisher: Oroboros Instruments Measurement of mitochondrial ATP production using Magnesium Green: DL-Protocols and data analysis with DatLab 7.4 Author(s): Cardoso LHD, Komlodi T, Gnaiger E Published in: Mitochondrial Physiology Network, Issue 26.10, 2021 Publisher: Oroboros Instruments NextGen-O2k: NADH-Module manual Author(s): Doerrier C, Schwaninger H, Walter-Vracevic M, Haider M, Niedenzu W, Komlódi T, Gradl P, Gnaiger E Published in: Mitochondrial Physiology Network, Issue 26.12, 2021 Publisher: Oroboros Instruments Hippocampal growth factor and myokine cathepsin B expression following aerobic and resistance training in 3xTg-AD mice Author(s): Cecatto C and Tindle-Solomon L Published in: O2k-brief, Issue 18/09/2020, 2020 Publisher: Oroboros Instruments Adaptation of mitochondrial substrate flux in a mouse model of nonalcoholic fatty liver disease Author(s): Komlodi T and Tindle-Solomon L Published in: O2k-brief, Issue 04/01/2021, 2021 Publisher: Oroboros Instruments An mtDNA mutant mouse demonstrates that mitochondrial deficiency can result in autism endophenotypes Author(s): Komlodi T and Tindle-Solomon L Published in: O2k-brief, Issue 29/03/2021, 2021 Publisher: Oroboros Instruments Ex vivo mitochondrial respiration parallels biochemical response to ibrutinib in CLL cells Author(s): Komlodi T and Tindle-Solomon L Published in: O2k-brief, Issue 16/08/2021, 2021 Publisher: Oroboros Instruments Oxygen dependence of photosynthesis and light-enhanced dark respiration studied by High-Resolution PhotoRespirometry Author(s): Went N, Di Marcello M, Gnaiger E Published in: MitoFit Preprints, Issue 2021.5, 2021 Publisher: Oroboros Instruments The elementary unit — canonical reviewer's comments on: Bureau International des Poids et Mesures Author(s): Gnaiger E Published in: MitoFit Preprints, Issue 2021.4(v2), 2021 Publisher: Oroboros Instruments Colon cancer cell differentiation by sodium butyrate modulates metabolic plasticity of Caco-2 cells via alteration of phosphotransfer network Author(s): Komlodi T and Tindle-Solomon L Published in: O2k-brief, Issue 09/06/2021, 2019 Publisher: Oroboros Instruments Titration-Injection microPump TIP2k manual. Author(s): Gradl L, Gradl P, Gnaiger E Published in: Mitochondrial Physiology Network, Issue 12.10(v9), 2021 Publisher: Oroboros Instruments Coupling and pathway control of coenzyme Q redox state and respiration in isolated mitochondria Author(s): Komlódi T, Cardoso LHD, Doerrier C, Moore AL, Rich PR, Gnaiger E Published in: MitoFit Preprints, Issue 2021.2(v4), 2021 Publisher: Oroboros Instruments Mitochondrial respiration of platelets: comparison of isolation methods. Author(s): Vernerova A, Garcia-Souza LF, Soucek O, Kostal M, Rehacek V, Krcmova LK, Gnaiger E, Sobotka O Published in: MitoFit Preprints, Issue 2021.6, 2021 Publisher: Oroboros Instruments Monographic books (1) Mitochondrial pathways and respiratory control Author(s): Gnaiger E Published in: Bioenergetics Communicatoins, Issue 2020.2, 2020, ISBN 978-3-9504814-2-6 Publisher: Bioenergetics Communicatoins Searching for OpenAIRE data... There was an error trying to search data from OpenAIRE No results available
