Proton transport and proton-coupled transport

Información del proyecto

PROTON

Identificador del acuerdo de subvención: 860592

DOI

10.3030/860592

Proyecto cerrado

Fecha de la firma de la CE 9 Agosto 2019

Fecha de inicio 1 Septiembre 2019

Fecha de finalización 31 Julio 2024

Financiado con arreglo a

• EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions

Coste total

€ 3 920 811,13

Aportación de la UE

€ 3 920 811,13

Coordinado por

UNIVERSITAT LINZ
Austria

CORDIS proporciona enlaces a los documentos públicos y las publicaciones de los proyectos de los programas marco HORIZONTE.

Los enlaces a los documentos y las publicaciones de los proyectos del Séptimo Programa Marco, así como los enlaces a algunos tipos de resultados específicos, como conjuntos de datos y «software», se obtienen dinámicamente de OpenAIRE .

Resultado final

Other (8)

Final International conference

A final international conference with all PIs and PhDs is organized

Time lapse movies of protons crossing the membrane and spreading on the receiving interface

Experiments in the presence of a transmembrane proton gradient are performed and time driven changes of the proton concentration are imaged

PROTON vacancies published

The PhD positions are advertised

Websites (external and internal)

A website for the proton consortium is designed and mounted

Scientific courses

Training schools providing scientific courses will be conducted

Time lapse movies of traveling proton fronts along the hydration shells of lipid bilayers

Timedependent changes of proton concentration gradients on top of a lipid bilayer are imaged

Transferable skill courses

Training schools providing transferable skills courses will be conducted

Overall recruitment

Recruitment of 15 ESRs and their enrolment in a Doctoral Program

Documents, reports (10)

Researcher Declarations on Conformity

Personal data and data related to the project allowances of the recruited PhD students are declared

Cysteine-labelled, pH-responsive HpUreI fluorescence

Functional investigations are performed to obtain insight into the gating mechanism of HpUreI

Model of FNT substrate-proton co-transport

The results of in vitro and in silico investigations of solute and proton cotransport are merged into a model

Catalogue of suitable combinations of titratable lipid residues and amino acid side chains for proton transfer from the interface into the pore of the transporter

The lipid dependence of proton transfer from the membrane surface into the membrane transporter is investigated

Ranking of different surface structures/intraluminal transporter structures according to their ability to conduct protons

We measure proton fluxes along different surfaces in vitro and in silico and compare the specific conductances

15 Doctoral theses submitted

The ESRs summarize their results in PhD theses

Inhibitors for artificial proton channels

Small molecules that are capable of inhibiting facilitated proton conductance are identified

Mechanistic model for ions (protons) moving in the hydration layers of collagen

The results of in vitro and in silico measurements of proton conductance along collagen are summarized by a model

Comprehensive Education Program Report

The effectiveness of the training program will be reported at the end of the project, to be used as a reference for structuring future multidisciplinary training programs.

Model of pH-dependent conformational changes during GPR68 signalling

Insight into the mechanism of pH sensing is obtained

Publicaciones

Peer reviewed articles (32)

Protons at bio-interfaces

Autores: Ana-Nicoleta Bondar; Mihail Barboiu
Publicado en: Crossref, Edición 1, 2023, ISSN 0005-2736
Editor: Elsevier BV
DOI: 10.1016/j.bbamem.2023.184139

Size dependence of second-harmonic scattering from nanoparticles: Disentangling surface and electrostatic contributions

Autores: Bingxin Chu; Arianna Marchioro; Sylvie Roke
Publicado en: Crossref, Edición 5, 2023, ISSN 0021-9606
Editor: American Institute of Physics
DOI: 10.1063/5.0135157

Imaging Cu 2+ binding to charged phospholipid membranes by high-throughput second harmonic wide-field microscopy

Autores: Seonwoo Lee, David Roesel, Sylvie Roke
Publicado en: The Journal of Chemical Physics, Edición 155/18, 2021, Página(s) 184704, ISSN 0021-9606
Editor: American Institute of Physics
DOI: 10.1063/5.0063362

Trapped pore waters in the open proton channel H_V1

Autores: Danila Boytsov; Stefania Brescia; Gustavo Chaves; Sabina Koefler; Christof Hannesschlaeger; Christine Siligan; Nikolaus Gössweiner-Mohr; Boris Musset; Peter Pohl
Publicado en: Crossref, Edición 10, 2023, ISSN 1613-6810
Editor: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.1101/2022.05.09.491141

Fluorescence Cross-Correlation Spectroscopy Yields True Affinity and Binding Kinetics of Plasmodium Lactate Transport Inhibitors

Autores: Iga Jakobowska, Frank Becker, Stefano Minguzzi, Kerrin Hansen, Björn Henke, Nathan Hugo Epalle, Eric Beitz, Stefan Hannus
Publicado en: Pharmaceuticals, Edición 14/8, 2021, Página(s) 757, ISSN 1424-8247
Editor: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ph14080757

Proton-Binding Motifs of Membrane-Bound Proteins: From Bacteriorhodopsin to Spike Protein S

Autores: Ana-Nicoleta Bondar
Publicado en: Frontiers in Chemistry, Vol 9 (2021), Edición 1, 2021, ISSN 2296-2646
Editor: frontiers in chemistry
DOI: 10.3389/fchem.2021.685761

Biomimetic Artificial Proton Channels

Autores: Andrei I-M, Barboiu M.
Publicado en: Biomolecules, Edición 2218273X, 2022, Página(s) 1473, ISSN 2218-273X
Editor: MDPI
DOI: 10.3390/biom12101473

A New Theory About Interfacial Proton Diffusion Revisited: The Commonly Accepted Laws of Electrostatics and Diffusion Prevail

Autores: Denis G. Knyazev; Todd P. Silverstein; Stefania Brescia; Anna Maznichenko; Peter Pohl
Publicado en: Biomolecules, Vol 13, Iss 11, p 1641 (2023), Edición 8, 2023, ISSN 2218-273X
Editor: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.2139/ssrn.4508746

Basigin drives intracellular accumulation of l-lactate by harvesting protons and substrate anions

Autores: Anna-Lena Köpnick, Annika Jansen, Katharina Geistlinger, Nathan Hugo Epalle, Eric Beitz
Publicado en: PLOS ONE, Edición 16/3, 2021, Página(s) e0249110, ISSN 1932-6203
Editor: Public Library of Science
DOI: 10.1371/journal.pone.0249110

Local Attraction of Substrates and Co-Substrates Enhances Weak Acid and Base Transmembrane Transport

Autores: Epalle, N. H. Beitz, E.
Publicado en: Biomolecules, 2022, ISSN 2218-273x
Editor: MDPI
DOI: 10.3390/biom12121794

Evolution of the electrical double layer with electrolyte concentration probed by second harmonic scattering

Autores: Bingxin Chu, Denys Biriukov, Marie Bischoff, Milan Předota, Sylvie Roke, Arianna Marchioro
Publicado en: Faraday Discussions, Edición 246, 2024, Página(s) 407-425, ISSN 1359-6640
Editor: Royal Society of Chemistry
DOI: 10.1039/d3fd00036b

Graph-Based Analyses of Dynamic Water-Mediated Hydrogen-Bond Networks in Phosphatidylserine: Cholesterol Membranes

Autores: Jain, Honey; Karathanou, Konstantina; Bondar, Ana-Nicoleta
Publicado en: Biomolecules 13(8), 1238 - (2023). doi:10.3390/biom13081238, Edición 6, 2023, ISSN 2218-273X
Editor: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/biom13081238

Dissecting the mechanisms of environment sensitivity of smart probes for quantitative assessment of membrane properties

Autores: Franziska Ragaller, Luca Andronico, Jan Sykora, Waldemar Kulig, Tomasz Rog†, Yagmur Balim Urem, Abhinav, Dmytro I. Danylchuk, Martin Hof, Andrey Klymchenko, Mariana Amaro, Ilpo Vattulainen and Erdinc Sezgin
Publicado en: Open Biology, 2022, ISSN 2046-2441
Editor: Royal Society Publishing
DOI: 10.1098/rsob.220175

Collagen Structured Hydration

Autores: Biswal, Satyaranjan Agmon, Noam
Publicado en: Biomolecules, 2023, ISSN 2218-273X
Editor: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/biom13121744

Uncoupling Protein 3 Catalyzes the Exchange of C4 Metabolites Similar to UCP2

Autores: Jürgen Kreiter; Tatyana Tyschuk; Elena E Pohl
Publicado en: Biomolecules, Vol 14, Iss 1, p 21 (2023), Edición 7, 2024, ISSN 2218-273X
Editor: MDPI
DOI: 10.3390/biom14010021

Hydrogen-bond networks for proton couplings in G-Protein coupled receptors

Autores: Ana-Nicoleta Bondar; Ana-Nicoleta Bondar; Mercedes Alfonso-Prieto
Publicado en: Frontiers in physics 10, 963716 (2022). doi:10.3389/fphy.2022.963716, Edición 5, 2022, ISSN 2296-424X
Editor: Frontiers in Physics
DOI: 10.3389/fphy.2022.963716

Does the ionic distribution in the electrical double layer modify second harmonic scattering?

Autores: Bingxin Chu, Sylvie Roke, Arianna Marchioro
Publicado en: The Journal of Chemical Physics, Edición 160, 2024, ISSN 0021-9606
Editor: American Institute of Physics
DOI: 10.1063/5.0198247

Dynamic Second Harmonic Imaging of Proton Translocation Through Water Needles in Lipid Membranes

Autores: Lee, Seonwoo Poojari, Chetan S. Maznichenko, Anna Roesel, David Swiderska, Iwona Pohl, Peter Hub, Jochen S. Roke, Sylvie
Publicado en: Journal of the American Chemical Society, 2024, ISSN 0002-7863
Editor: American Chemical Society
DOI: 10.1021/jacs.4c02810

Modulation of Anionic Lipid Bilayers by Specific Interplay of Protons and Calcium Ions

Autores: Abhinav, Jurkiewicz P, Hof M, Allolio C, Sýkora J.
Publicado en: Biomolecules, 2022, ISSN 2218-273X
Editor: mdpi
DOI: 10.3390/biom12121894

Discovery and Development of Inhibitors of the Plasmodial FNT-Type Lactate Transporter as Novel Antimalarials

Autores: Cornelius Nerlich, Nathan H. Epalle, Philip Seick, Eric Beitz
Publicado en: Pharmaceuticals, Edición 14/11, 2021, Página(s) 1191, ISSN 1424-8247
Editor: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ph14111191

Self-Assembled H-Bonding Superstructures for Alkali Cation and Proton Transport.

Autores: Erol Licsandru; Iuliana-Marilena Andrei; Arie van der Lee; Mihail Barboiu
Publicado en: https://hal.archives-ouvertes.fr/hal-03410221, Edición 4, 2021, ISSN 2296-2646
Editor: Front Chem
DOI: 10.3389/fchem.2021.678962

Cysteine 159 delineates a hinge region of the alternating access monocarboxylate transporter 1 and is targeted by cysteine-modifying inhibitors.

Autores: Anna-Lena Köpnick; Katharina Geistlinger; Eric Beitz
Publicado en: The FEBS Journal, Edición 2, 2021, ISSN 1742-464X
Editor: Blackwell Publishing Inc.
DOI: 10.1111/febs.16024

Lactic Acid Permeability of Aquaporin-9 Enables Cytoplasmic Lactate Accumulation via an Ion Trap

Autores: Katharina Geistlinger; Jana D. R. Schmidt; Eric Beitz
Publicado en: Life; Volume 12; Edición 1; Pages: 120, Edición 2, 2022, ISSN 2075-1729
Editor: life
DOI: 10.3390/life12010120

Selective Water Pore Recognition and Transport through Self-Assembled Alkyl-Ureido-Trianglamine Artificial Water Channels

Autores: Iuliana M. Andrei; Arnaud Chaix; Belkacem Tarek Benkhaled; Romain Dupuis; Chaimaa Gomri; Eddy Petit; Maurizio Polentarutti; Arie van der Lee; Mona Semsarilar; Mihail Barboiu
Publicado en: Journal of the American Chemical Society, Edición 3, 2023, ISSN 0002-7863
Editor: American Chemical Society
DOI: 10.1021/jacs.3c02815

Proton- versus Cation-Selective Transport of Saccharide Rim-Appended Pillar[5]arene Artificial Water Channels

Autores: Iuliana M. Andrei, Wenzhang Chen, Marc Baaden, Stéphane P. Vincent, Mihail Barboiu
Publicado en: Journal of the American Chemical Society, Edición 145, 2023, Página(s) 21904-21914, ISSN 0002-7863
Editor: American Chemical Society
DOI: 10.1021/jacs.3c06335

Mitochondrial Uncoupling Proteins (UCP1-UCP3) and Adenine Nucleotide Translocase (ANT1) Enhance the Protonophoric Action of 2,4-Dinitrophenol in Mitochondria and Planar Bilayer Membranes

Autores: Kristina Žuna, Olga Jovanović, Ljudmila S Khailova, Sanja Škulj , Zlatko Brkljača , Jürgen Kreiter, Elena A Kotova , Mario Vazdar , Yuri N Antonenko, Elena E Pohl
Publicado en: Biomolecules, Edición 11/8, 2021, Página(s) 1178, ISSN 2218-273X
Editor: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/biom11081178

Human monocarboxylate transporters accept and relay protons via the bound substrate for selectivity and activity at physiological pH

Autores: Katharina Geistlinger; Jana D R Schmidt; Eric Beitz
Publicado en: Crossref, Edición 1, 2023, ISSN 2752-6542
Editor: PMC
DOI: 10.1093/pnasnexus/pgad007

Combinatorial Screening of Water/Proton Permeation of Self‐Assembled Pillar[5]arene Artificial Water Channel Libraries

Autores: Iuliana‐Marilena Andrei; Dmytro Strilets; Shixin Fa; Marc Baaden; Tomoki Ogoshi; Mihail Barboiu
Publicado en: https://hal.science/hal-04257786, Edición 1, 2023, ISSN 1433-7851
Editor: John Wiley & Sons Ltd.
DOI: 10.1002/anie.202310812

Dual-Probe Activity-Based Protein Profiling Reveals Site-Specific Differences in Protein Binding of EGFR-Directed Drugs

Autores: Wouter van Bergen, Kristina Žuna, Jan Fiala, Elena E. Pohl, Albert J.R. Heck, Marc P. Baggelaar
Publicado en: ACS Chemical Biology, Edición 19, 2024, Página(s) 1705-1718, ISSN 1554-8929
Editor: American Chemical Society
DOI: 10.1021/acschembio.3c00637

Water as a contrast agent to quantify surface chemistry and physics using second harmonic scattering and imaging: A perspective

Autores: D. Roesel; M. Eremchev; T. Schönfeldová; S. Lee; S. Roke
Publicado en: Appl. Phys. Lett., Edición 12, 2022, ISSN 0003-6951
Editor: American Institute of Physics
DOI: 10.1063/5.0085807

Mutational widening of constrictions in a formate–nitrite/H+ transporter enables aquaporin-like water permeability and proton conductance

Autores: Jana D.R. Schmidt, Eric Beitz
Publicado en: Journal of Biological Chemistry, Edición 298, 2022, Página(s) 101513, ISSN 0021-9258
Editor: American Society for Biochemistry and Molecular Biology Inc.
DOI: 10.1016/j.jbc.2021.101513

Protons taken hostage: Dynamic H-bond networks of the pH-sensing GPR68

Autores: Kapur, B.; Baldessari, F.; Lazaratos, M.; Nar, H.; Schnapp, G.; Giorgetti, A.; Bondar, A.-N.
Publicado en: Computational and Structural Biotechnology Journal, Edición 21, 2023, Página(s) 4370-4384, ISSN 2001-0370
Editor: Elsevier
DOI: 10.1016/j.csbj.2023.08.034

Book chapters (2)

Mechanisms of long-distance allosteric couplings in proton-binding membrane transporters

Autores: Ana-Nicoleta Bondar
Publicado en: Crossref, Edición 3, 2022, ISSN 1876-1623
Editor: Adv Protein Chem Struct Biol
DOI: 10.1016/bs.apcsb.2021.09.002

Dynamics and Hydration of Proteins Viewed by Fluorescence Methods: Investigations for Protein Engineering and Synthetic Biology

Autores: Sykora, J., Prokop, Z., Damborsky, J., Abhinav, Hof, M., Amaro, M.
Publicado en: Springer Series on Fluorescence, 2023
Editor: Springer
DOI: 10.1007/4243_2022_40