Proton transport and proton-coupled transport

Informazioni relative al progetto

PROTON

ID dell’accordo di sovvenzione: 860592

DOI

10.3030/860592

Progetto chiuso

Data della firma CE 9 Agosto 2019

Data di avvio 1 Settembre 2019

Data di completamento 31 Luglio 2024

Finanziato da

• EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions

Costo totale

€ 3 920 811,13

Contributo UE

€ 3 920 811,13

Coordinato da

UNIVERSITAT LINZ
Austria

CORDIS fornisce collegamenti ai risultati finali pubblici e alle pubblicazioni dei progetti ORIZZONTE.

I link ai risultati e alle pubblicazioni dei progetti del 7° PQ, così come i link ad alcuni tipi di risultati specifici come dataset e software, sono recuperati dinamicamente da .OpenAIRE .

Risultati finali

Other (8)

Final International conference

A final international conference with all PIs and PhDs is organized

Time lapse movies of protons crossing the membrane and spreading on the receiving interface

Experiments in the presence of a transmembrane proton gradient are performed and time driven changes of the proton concentration are imaged

PROTON vacancies published

The PhD positions are advertised

Websites (external and internal)

A website for the proton consortium is designed and mounted

Scientific courses

Training schools providing scientific courses will be conducted

Time lapse movies of traveling proton fronts along the hydration shells of lipid bilayers

Timedependent changes of proton concentration gradients on top of a lipid bilayer are imaged

Transferable skill courses

Training schools providing transferable skills courses will be conducted

Overall recruitment

Recruitment of 15 ESRs and their enrolment in a Doctoral Program

Documents, reports (10)

Researcher Declarations on Conformity

Personal data and data related to the project allowances of the recruited PhD students are declared

Cysteine-labelled, pH-responsive HpUreI fluorescence

Functional investigations are performed to obtain insight into the gating mechanism of HpUreI

Model of FNT substrate-proton co-transport

The results of in vitro and in silico investigations of solute and proton cotransport are merged into a model

Catalogue of suitable combinations of titratable lipid residues and amino acid side chains for proton transfer from the interface into the pore of the transporter

The lipid dependence of proton transfer from the membrane surface into the membrane transporter is investigated

Ranking of different surface structures/intraluminal transporter structures according to their ability to conduct protons

We measure proton fluxes along different surfaces in vitro and in silico and compare the specific conductances

15 Doctoral theses submitted

The ESRs summarize their results in PhD theses

Inhibitors for artificial proton channels

Small molecules that are capable of inhibiting facilitated proton conductance are identified

Mechanistic model for ions (protons) moving in the hydration layers of collagen

The results of in vitro and in silico measurements of proton conductance along collagen are summarized by a model

Comprehensive Education Program Report

The effectiveness of the training program will be reported at the end of the project, to be used as a reference for structuring future multidisciplinary training programs.

Model of pH-dependent conformational changes during GPR68 signalling

Insight into the mechanism of pH sensing is obtained

Pubblicazioni

Peer reviewed articles (32)

Protons at bio-interfaces

Autori: Ana-Nicoleta Bondar; Mihail Barboiu
Pubblicato in: Crossref, Numero 1, 2023, ISSN 0005-2736
Editore: Elsevier BV
DOI: 10.1016/j.bbamem.2023.184139

Size dependence of second-harmonic scattering from nanoparticles: Disentangling surface and electrostatic contributions

Autori: Bingxin Chu; Arianna Marchioro; Sylvie Roke
Pubblicato in: Crossref, Numero 5, 2023, ISSN 0021-9606
Editore: American Institute of Physics
DOI: 10.1063/5.0135157

Imaging Cu 2+ binding to charged phospholipid membranes by high-throughput second harmonic wide-field microscopy

Autori: Seonwoo Lee, David Roesel, Sylvie Roke
Pubblicato in: The Journal of Chemical Physics, Numero 155/18, 2021, Pagina/e 184704, ISSN 0021-9606
Editore: American Institute of Physics
DOI: 10.1063/5.0063362

Trapped pore waters in the open proton channel H_V1

Autori: Danila Boytsov; Stefania Brescia; Gustavo Chaves; Sabina Koefler; Christof Hannesschlaeger; Christine Siligan; Nikolaus Gössweiner-Mohr; Boris Musset; Peter Pohl
Pubblicato in: Crossref, Numero 10, 2023, ISSN 1613-6810
Editore: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.1101/2022.05.09.491141

Fluorescence Cross-Correlation Spectroscopy Yields True Affinity and Binding Kinetics of Plasmodium Lactate Transport Inhibitors

Autori: Iga Jakobowska, Frank Becker, Stefano Minguzzi, Kerrin Hansen, Björn Henke, Nathan Hugo Epalle, Eric Beitz, Stefan Hannus
Pubblicato in: Pharmaceuticals, Numero 14/8, 2021, Pagina/e 757, ISSN 1424-8247
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ph14080757

Proton-Binding Motifs of Membrane-Bound Proteins: From Bacteriorhodopsin to Spike Protein S

Autori: Ana-Nicoleta Bondar
Pubblicato in: Frontiers in Chemistry, Vol 9 (2021), Numero 1, 2021, ISSN 2296-2646
Editore: frontiers in chemistry
DOI: 10.3389/fchem.2021.685761

Biomimetic Artificial Proton Channels

Autori: Andrei I-M, Barboiu M.
Pubblicato in: Biomolecules, Numero 2218273X, 2022, Pagina/e 1473, ISSN 2218-273X
Editore: MDPI
DOI: 10.3390/biom12101473

A New Theory About Interfacial Proton Diffusion Revisited: The Commonly Accepted Laws of Electrostatics and Diffusion Prevail

Autori: Denis G. Knyazev; Todd P. Silverstein; Stefania Brescia; Anna Maznichenko; Peter Pohl
Pubblicato in: Biomolecules, Vol 13, Iss 11, p 1641 (2023), Numero 8, 2023, ISSN 2218-273X
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.2139/ssrn.4508746

Basigin drives intracellular accumulation of l-lactate by harvesting protons and substrate anions

Autori: Anna-Lena Köpnick, Annika Jansen, Katharina Geistlinger, Nathan Hugo Epalle, Eric Beitz
Pubblicato in: PLOS ONE, Numero 16/3, 2021, Pagina/e e0249110, ISSN 1932-6203
Editore: Public Library of Science
DOI: 10.1371/journal.pone.0249110

Local Attraction of Substrates and Co-Substrates Enhances Weak Acid and Base Transmembrane Transport

Autori: Epalle, N. H. Beitz, E.
Pubblicato in: Biomolecules, 2022, ISSN 2218-273x
Editore: MDPI
DOI: 10.3390/biom12121794

Evolution of the electrical double layer with electrolyte concentration probed by second harmonic scattering

Autori: Bingxin Chu, Denys Biriukov, Marie Bischoff, Milan Předota, Sylvie Roke, Arianna Marchioro
Pubblicato in: Faraday Discussions, Numero 246, 2024, Pagina/e 407-425, ISSN 1359-6640
Editore: Royal Society of Chemistry
DOI: 10.1039/d3fd00036b

Graph-Based Analyses of Dynamic Water-Mediated Hydrogen-Bond Networks in Phosphatidylserine: Cholesterol Membranes

Autori: Jain, Honey; Karathanou, Konstantina; Bondar, Ana-Nicoleta
Pubblicato in: Biomolecules 13(8), 1238 - (2023). doi:10.3390/biom13081238, Numero 6, 2023, ISSN 2218-273X
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/biom13081238

Dissecting the mechanisms of environment sensitivity of smart probes for quantitative assessment of membrane properties

Autori: Franziska Ragaller, Luca Andronico, Jan Sykora, Waldemar Kulig, Tomasz Rog†, Yagmur Balim Urem, Abhinav, Dmytro I. Danylchuk, Martin Hof, Andrey Klymchenko, Mariana Amaro, Ilpo Vattulainen and Erdinc Sezgin
Pubblicato in: Open Biology, 2022, ISSN 2046-2441
Editore: Royal Society Publishing
DOI: 10.1098/rsob.220175

Collagen Structured Hydration

Autori: Biswal, Satyaranjan Agmon, Noam
Pubblicato in: Biomolecules, 2023, ISSN 2218-273X
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/biom13121744

Uncoupling Protein 3 Catalyzes the Exchange of C4 Metabolites Similar to UCP2

Autori: Jürgen Kreiter; Tatyana Tyschuk; Elena E Pohl
Pubblicato in: Biomolecules, Vol 14, Iss 1, p 21 (2023), Numero 7, 2024, ISSN 2218-273X
Editore: MDPI
DOI: 10.3390/biom14010021

Hydrogen-bond networks for proton couplings in G-Protein coupled receptors

Autori: Ana-Nicoleta Bondar; Ana-Nicoleta Bondar; Mercedes Alfonso-Prieto
Pubblicato in: Frontiers in physics 10, 963716 (2022). doi:10.3389/fphy.2022.963716, Numero 5, 2022, ISSN 2296-424X
Editore: Frontiers in Physics
DOI: 10.3389/fphy.2022.963716

Does the ionic distribution in the electrical double layer modify second harmonic scattering?

Autori: Bingxin Chu, Sylvie Roke, Arianna Marchioro
Pubblicato in: The Journal of Chemical Physics, Numero 160, 2024, ISSN 0021-9606
Editore: American Institute of Physics
DOI: 10.1063/5.0198247

Dynamic Second Harmonic Imaging of Proton Translocation Through Water Needles in Lipid Membranes

Autori: Lee, Seonwoo Poojari, Chetan S. Maznichenko, Anna Roesel, David Swiderska, Iwona Pohl, Peter Hub, Jochen S. Roke, Sylvie
Pubblicato in: Journal of the American Chemical Society, 2024, ISSN 0002-7863
Editore: American Chemical Society
DOI: 10.1021/jacs.4c02810

Modulation of Anionic Lipid Bilayers by Specific Interplay of Protons and Calcium Ions

Autori: Abhinav, Jurkiewicz P, Hof M, Allolio C, Sýkora J.
Pubblicato in: Biomolecules, 2022, ISSN 2218-273X
Editore: mdpi
DOI: 10.3390/biom12121894

Discovery and Development of Inhibitors of the Plasmodial FNT-Type Lactate Transporter as Novel Antimalarials

Autori: Cornelius Nerlich, Nathan H. Epalle, Philip Seick, Eric Beitz
Pubblicato in: Pharmaceuticals, Numero 14/11, 2021, Pagina/e 1191, ISSN 1424-8247
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ph14111191

Self-Assembled H-Bonding Superstructures for Alkali Cation and Proton Transport.

Autori: Erol Licsandru; Iuliana-Marilena Andrei; Arie van der Lee; Mihail Barboiu
Pubblicato in: https://hal.archives-ouvertes.fr/hal-03410221, Numero 4, 2021, ISSN 2296-2646
Editore: Front Chem
DOI: 10.3389/fchem.2021.678962

Cysteine 159 delineates a hinge region of the alternating access monocarboxylate transporter 1 and is targeted by cysteine-modifying inhibitors.

Autori: Anna-Lena Köpnick; Katharina Geistlinger; Eric Beitz
Pubblicato in: The FEBS Journal, Numero 2, 2021, ISSN 1742-464X
Editore: Blackwell Publishing Inc.
DOI: 10.1111/febs.16024

Lactic Acid Permeability of Aquaporin-9 Enables Cytoplasmic Lactate Accumulation via an Ion Trap

Autori: Katharina Geistlinger; Jana D. R. Schmidt; Eric Beitz
Pubblicato in: Life; Volume 12; Numero 1; Pages: 120, Numero 2, 2022, ISSN 2075-1729
Editore: life
DOI: 10.3390/life12010120

Selective Water Pore Recognition and Transport through Self-Assembled Alkyl-Ureido-Trianglamine Artificial Water Channels

Autori: Iuliana M. Andrei; Arnaud Chaix; Belkacem Tarek Benkhaled; Romain Dupuis; Chaimaa Gomri; Eddy Petit; Maurizio Polentarutti; Arie van der Lee; Mona Semsarilar; Mihail Barboiu
Pubblicato in: Journal of the American Chemical Society, Numero 3, 2023, ISSN 0002-7863
Editore: American Chemical Society
DOI: 10.1021/jacs.3c02815

Proton- versus Cation-Selective Transport of Saccharide Rim-Appended Pillar[5]arene Artificial Water Channels

Autori: Iuliana M. Andrei, Wenzhang Chen, Marc Baaden, Stéphane P. Vincent, Mihail Barboiu
Pubblicato in: Journal of the American Chemical Society, Numero 145, 2023, Pagina/e 21904-21914, ISSN 0002-7863
Editore: American Chemical Society
DOI: 10.1021/jacs.3c06335

Mitochondrial Uncoupling Proteins (UCP1-UCP3) and Adenine Nucleotide Translocase (ANT1) Enhance the Protonophoric Action of 2,4-Dinitrophenol in Mitochondria and Planar Bilayer Membranes

Autori: Kristina Žuna, Olga Jovanović, Ljudmila S Khailova, Sanja Škulj , Zlatko Brkljača , Jürgen Kreiter, Elena A Kotova , Mario Vazdar , Yuri N Antonenko, Elena E Pohl
Pubblicato in: Biomolecules, Numero 11/8, 2021, Pagina/e 1178, ISSN 2218-273X
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/biom11081178

Human monocarboxylate transporters accept and relay protons via the bound substrate for selectivity and activity at physiological pH

Autori: Katharina Geistlinger; Jana D R Schmidt; Eric Beitz
Pubblicato in: Crossref, Numero 1, 2023, ISSN 2752-6542
Editore: PMC
DOI: 10.1093/pnasnexus/pgad007

Combinatorial Screening of Water/Proton Permeation of Self‐Assembled Pillar[5]arene Artificial Water Channel Libraries

Autori: Iuliana‐Marilena Andrei; Dmytro Strilets; Shixin Fa; Marc Baaden; Tomoki Ogoshi; Mihail Barboiu
Pubblicato in: https://hal.science/hal-04257786, Numero 1, 2023, ISSN 1433-7851
Editore: John Wiley & Sons Ltd.
DOI: 10.1002/anie.202310812

Dual-Probe Activity-Based Protein Profiling Reveals Site-Specific Differences in Protein Binding of EGFR-Directed Drugs

Autori: Wouter van Bergen, Kristina Žuna, Jan Fiala, Elena E. Pohl, Albert J.R. Heck, Marc P. Baggelaar
Pubblicato in: ACS Chemical Biology, Numero 19, 2024, Pagina/e 1705-1718, ISSN 1554-8929
Editore: American Chemical Society
DOI: 10.1021/acschembio.3c00637

Water as a contrast agent to quantify surface chemistry and physics using second harmonic scattering and imaging: A perspective

Autori: D. Roesel; M. Eremchev; T. Schönfeldová; S. Lee; S. Roke
Pubblicato in: Appl. Phys. Lett., Numero 12, 2022, ISSN 0003-6951
Editore: American Institute of Physics
DOI: 10.1063/5.0085807

Mutational widening of constrictions in a formate–nitrite/H+ transporter enables aquaporin-like water permeability and proton conductance

Autori: Jana D.R. Schmidt, Eric Beitz
Pubblicato in: Journal of Biological Chemistry, Numero 298, 2022, Pagina/e 101513, ISSN 0021-9258
Editore: American Society for Biochemistry and Molecular Biology Inc.
DOI: 10.1016/j.jbc.2021.101513

Protons taken hostage: Dynamic H-bond networks of the pH-sensing GPR68

Autori: Kapur, B.; Baldessari, F.; Lazaratos, M.; Nar, H.; Schnapp, G.; Giorgetti, A.; Bondar, A.-N.
Pubblicato in: Computational and Structural Biotechnology Journal, Numero 21, 2023, Pagina/e 4370-4384, ISSN 2001-0370
Editore: Elsevier
DOI: 10.1016/j.csbj.2023.08.034

Book chapters (2)

Mechanisms of long-distance allosteric couplings in proton-binding membrane transporters

Autori: Ana-Nicoleta Bondar
Pubblicato in: Crossref, Numero 3, 2022, ISSN 1876-1623
Editore: Adv Protein Chem Struct Biol
DOI: 10.1016/bs.apcsb.2021.09.002

Dynamics and Hydration of Proteins Viewed by Fluorescence Methods: Investigations for Protein Engineering and Synthetic Biology

Autori: Sykora, J., Prokop, Z., Damborsky, J., Abhinav, Hof, M., Amaro, M.
Pubblicato in: Springer Series on Fluorescence, 2023
Editore: Springer
DOI: 10.1007/4243_2022_40