Dynamic adaptive microscopy for label-free multi-parametric imaging in biology and medicine

Información del proyecto

DynAMic

Identificador del acuerdo de subvención: 863203

DOI

10.3030/863203

Proyecto cerrado

Fecha de la firma de la CE 2 Septiembre 2019

Fecha de inicio 1 Enero 2020

Fecha de finalización 31 Diciembre 2024

Financiado con arreglo a

• EXCELLENT SCIENCE - Future and Emerging Technologies (FET)

Coste total

€ 3 957 137,50

Aportación de la UE

€ 3 957 137,50

Coordinado por

IDRYMA TECHNOLOGIAS KAI EREVNAS
Greece

CORDIS proporciona enlaces a los documentos públicos y las publicaciones de los proyectos de los programas marco HORIZONTE.

Los enlaces a los documentos y las publicaciones de los proyectos del Séptimo Programa Marco, así como los enlaces a algunos tipos de resultados específicos, como conjuntos de datos y «software», se obtienen dinámicamente de OpenAIRE .

Resultado final

Demonstrators, pilots, prototypes (7)

Optimization algorithms

Development of initial optimization and transmission matrix calculation algorithms. New concepts to account for refractive index variations with adaptive meshing, Levy statistics and Monte Carlo modelling of light propagation in complex media

Phase retrieval

Development of 3D autocorrelation phase and polarization retrieval algorithms exploiting coherent multiply scattered light in optical, optoacoustic, non-linear and Raman methods

Non-diffracting illumination

Non-diffracting beam formation strategies with appropriate spatial filtering (reconfigurable photonic, biological and biomimetic structures) and coherent structured and speckle illumination

Adaptive module

Combine ultrafast wavefront shaping adaptive optomechatronics technologies (build in WP3 and WP4) in one microscopy enabled module

SRS full integration

Build coherent stimulated Raman Scattering (SRS) ability for fast identification of molecular fingerprints (identified in Task 6.2)

Integrated system

Integrate the different technologies and methods in a single prototype microscopy system, optimizing its operational characteristics in terms of sensitivity and specificity and identify safety appropriateness for animal and human use

Molecular/Proteomic fingerprints

Steer morphological & molecular fingerprinting (Raman) towards clinically related targets / questions by adapted fast Stimulated Raman Scattering (SRS) against the identified proteomic biomarkers (built in Task 5.3)

Documents, reports (6)

Raman analysis

Using a conventional Raman imaging system to analyse spectral signatures in relation to proteomic knowledge and identify molecules/proteins of clinical interest

In vivo assessment

Assess in vivo imaging ability of system in animals (rat, rabbit and pig; years 3, 4) and clinical relevance of new techniques and markers derived thereof in comparison with established clinical procedures and diagnostic markers

Dissemination and Exploitation final plan

Implemented actions of our dissemination, exploitation plan, and IPR management. Our industrial partners Rayfos and Imagine Optic and the DC have been appointed for coordinating this highly important task

Dissemination and Exploitation plan

Design of our dissemination, exploitation plan and IPR management. Our industrial partners Rayfos and Imagine Optic and the DC have been appointed for coordinating this highly important task

Dissemination and Exploitation interim plan

Implemented actions of our dissemination exploitation plan and IPR management Our industrial partners Rayfos and Imagine Optic and the DC have been appointed for coordinating this highly important task

Proof of concept

Proof of concept imaging and assessment of imaging prototype with freshly excised animal tissue samples retina cancer spheroids

Other (1)

Summer school

FORTH will organize a summer school on Biophotonics in Life Sciences, taking place on month 24. All involved parties will participate and leading scientists will be invited to teach the current state of the art as well as complimentary skills (IP management, entrepreneurship, proposal writing etc).

Websites, patent fillings, videos etc. (1)

Website/Logo

Design of the project's logo and realization of the dedicated webpage with public sector and protected inranet for partners access only

Open Research Data Pilot (1)

Data management

Report on the data management plan of the consortium

Publicaciones

Peer reviewed articles (45)

Large field-of-view non-invasive imaging through scattering layers using fluctuating random illumination

Autores: Lei Zhu, Fernando Soldevila, , Claudio Moretti, Alexandra d’Arco, Antoine Boniface, Xiaopeng Shao, Hilton B. de Aguiar & Sylvain Gigan
Publicado en: Nature Communications, 2022, ISSN 2041-1723
Editor: Nature Publishing Group
DOI: 10.1038/s41467-022-29166-y

Spatially offset optical coherence tomography: Leveraging multiple scattering for high-contrast imaging at depth in turbid media

Autores: GavrielleR. Untracht, MingzhouChen, PhilipWijesinghe, JosepMas, Harold T. Yura3, DominikMarti, PeterE. Andersen, KishanDholakia
Publicado en: Science Advances, 2023, ISSN 2375-2548
Editor: American Association for the Advancement of Science
DOI: 10.1126/sciadv.adh5435

Hybrid confocal fluorescence and photoacoustic microscopy for the label-free investigation of melanin accumulation in fish scales

Autores: George J. Tserevelakis, Michalis Pavlidis, Athanasios Samaras, Georgios D. Barmparis, Kostas G. Mavrakis, Ioannis Draganidis, Athanasios Oikonomou, Eleftheria Fanouraki, Giorgos P. Tsironis & Giannis Zacharakis
Publicado en: Scientific Reports, Edición Edición 12, 2022, ISSN 2045-2322
Editor: Nature Publishing Group
DOI: 10.1038/s41598-022-11262-0

Stratigraphy of Fresco Paintings: A New Approach with Photoacoustic and SORS Imaging

Autores: Francesca A. Pisu , Daniele Chiriu Evgenia Klironomou, Giannis Zacharakis and George J. Tserevelakis
Publicado en: Journal of Imaging, 2023, ISSN 2313-433X
Editor: Francesca A. Pisu
DOI: 10.3390/jimaging9010016

Sidelobe suppressed Bessel beams for one-photon light-sheet microscopy

Autores: Chetna Taneja, Jerin Geogy George, Stella Corsetti, Philip Wijesinghe, Graham D. Bruce, Maarten F. Zwart, Shanti Bhattacharya, Kishan Dholakia
Publicado en: Biomedical Optics Express, Edición 15, 2024, Página(s) 6183, ISSN 2156-7085
Editor: The Optical Society
DOI: 10.1364/boe.538253

Complement Factor H Loss in RPE Cells Causes Retinal Degeneration in a Human RPE-Porcine Retinal Explant Co-Culture Model

Autores: Angela Armento, Aparna Murali, Julia Marzi, Ana C Almansa-Garcia, Blanca Arango-Gonzalez, Ellen Kilger, Simon J Clark, Katja Schenke-Layland, Charmaine A Ramlogan-Steel, Jason C Steel, Marius Ueffing
Publicado en: Biomolecules, Edición 11, 2024, Página(s) 1621, ISSN 2218-273X
Editor: MDPI
DOI: 10.3390/biom11111621

Online learning of the transmission matrix of dynamic scattering media

Autores: Lorenzo Valzania, Sylvain Gigan
Publicado en: Optica, Edición 10, 2024, Página(s) 708, ISSN 2334-2536
Editor: Optica
DOI: 10.1364/optica.479962

Optically generated droplet beams improve optoacoustic imaging of choroid thickness as an Alzheimer’s disease biomarker

Autores: Kostas G. Mavrakis, Gerasimos Divaris, Maria Tampakaki, Saba N. Khan, Kishan Dholakia, Giannis Zacharakis
Publicado en: npj Nanophotonics, Edición 1, 2024, ISSN 2948-216X
Editor: nature
DOI: 10.1038/s44310-024-00036-3

Roadmap on chaos-inspired imaging technologies (CI2-Tech)

Autores: Joseph Rosen, Hilton B. de Aguiar, Vijayakumar Anand, YoonSeok Baek, Sylvain Gigan, Ryoichi Horisaki, Hervé Hugonnet, Saulius Juodkazis, KyeoReh Lee, Haowen Liang, Yikun Liu, Stephan Ludwig, Wolfgang Osten, YongKeun Park, Giancarlo Pedrini, Tushar Sarkar, Johannes Schindler, Alok Kumar Singh, Rakesh Kumar Singh, Guohai Situ, Mitsuo Takeda, Xiangsheng Xie, Wanqin Yang, Jianying Zhou
Publicado en: Applied Physics B, Edición 128, 2022, ISSN 0946-2171
Editor: Springer Verlag
DOI: 10.1007/s00340-021-07729-z

A Cost-Efficient Multiwavelength LED-Based System for Quantitative Photoacoustic Measurements

Autores: Michalis Orfanakis, George J. Tserevelakis, Giannis Zacharakis
Publicado en: Sensors, Edición 21/14, 2021, Página(s) 4888, ISSN 1424-8220
Editor: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/s21144888

A proton-inhibited DEG/ENaC ion channel maintains neuronal ionstasis and promotes neuronal survival under stress

Autores: Dionysia Petratou, Martha Gjikolaj, Eva Kaulich, William Schafer, Nektarios Tavernarakis
Publicado en: iScience, 2023, ISSN 2589-0042
Editor: Dionysia Petratou
DOI: 10.1016/j.isci.2023.107117

To focus-match or not to focus-match inverse spatially offset Raman spectroscopy: a question of light penetration

Autores: Georgina E. Shillito, Lewis Mcmillan, Graham D. Bruce, and Kishan Dholakia
Publicado en: Optics Express, 2022, ISSN 1094-4087
Editor: Optical Society of America
DOI: 10.1364/oe.451496

Hybrid Raman and Partial Wave Spectroscopy Microscope for the Characterization of Molecular and Structural Alterations in Tissue

Autores: Elena Kriukova, Mikhail Mazurenka, Sabrina Marcazzan, Sarah Glasl, Michael Quante, Dieter Saur, Markus Tschurtschenthaler, Gerwin J. Puppels, Dimitris Gorpas, Vasilis Ntziachristos
Publicado en: Journal of Biophotonics, Edición 17, 2024, ISSN 1864-063X
Editor: Wiley - VCH Verlag GmbH & CO. KGaA
DOI: 10.1002/jbio.202400330

MitoSNARE Assembly and Disassembly Factors Regulate Basal Autophagy and Aging in C. elegans

Autores: Ilias Gkikas, Ioanna Daskalaki, Konstantinos Kounakis, Nektarios Tavernarakis, Eirini Lionaki
Publicado en: International Journal of Molecular Sciences, Edición 24, 2025, Página(s) 4230, ISSN 1422-0067
Editor: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms24044230

Physics-based neural network for non-invasive control of coherent light in scattering media

Autores: Alexandra d’Arco, Fei Xia, Antoine Boniface, Jonathan Dong, Sylvain Gigan
Publicado en: Optics Express, Edición 30, 2024, Página(s) 30845, ISSN 1094-4087
Editor: Optical Society of America
DOI: 10.1364/oe.465702

High precision photoacoustic interferometer for the determination of the speed of sound in liquid media

Autores: George J. Tserevelakis and Giannis Zacharakis
Publicado en: Optics Express, 2022
Editor: George J. Tserevelakis
DOI: 10.1364/oe.455557

Agar Gel as a Non-Invasive Coupling Medium for Reflectance Photoacoustic (PA) Imaging: Experimental Results on Wall-Painting Mock-Ups

Autores: Antonina Chaban , George J. Tserevelakis, Evgenia Klironomou, Giannis Zacharakis and Jana Striov
Publicado en: Journal of Imaging, 2022, ISSN 2313-433X
Editor: Antonina Chaban
DOI: 10.3390/jimaging8090235

Full image reconstruction in frequency-domain photoacoustic microscopy by means of a low-cost I/Q demodulator

Autores: George J. Tserevelakis, Kostas G. Mavrakis, Nikitas Kakakios, Giannis Zacharakis
Publicado en: Optics Letters, Edición 46/19, 2021, Página(s) 4718, ISSN 0146-9592
Editor: Optical Society of America
DOI: 10.1364/ol.435146

Scattering correcting wavefront shaping for three-photon microscopy

Autores: Bernhard Rauer, Hilton B. de Aguiar, Laurent Bourdieu, Sylvain Gigan
Publicado en: Optics Letters, Edición 47, 2024, Página(s) 6233, ISSN 0146-9592
Editor: Optical Society of America
DOI: 10.1364/ol.468834

Label-free super-resolution stimulated Raman scattering imaging of biomedical specimens

Autores: Julien Guilbert, Awoke Negash, Simon Labouesse, Sylvain Gigan, Anne Sentenac, Hilton B. de Aguiar
Publicado en: Advanced Imaging, Edición 1, 2024, Página(s) 011004, ISSN 2959-9539
Editor: Researchng
DOI: 10.3788/ai.2024.10004

PML Differentially Regulates Growth and Invasion in Brain Cancer.

Autores: Maria Tampakaki; Maria Tampakaki; Mariam-Eleni Oraiopoulou; Eleftheria Tzamali; Giorgos Tzedakis; Takis Makatounakis; Giannis Zacharakis; Joseph Papamatheakis; Joseph Papamatheakis; Vangelis Sakkalis
Publicado en: Edición 12, Edición 11, 2021, ISSN 1422-0067
Editor: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms22126289

Neuronal atg1 Coordinates Autophagy Induction and Physiological Adaptations to Balance mTORC1 Signalling

Autores: Athanasios Metaxakis Michail Pavlidis and Nektarios Tavernarakis
Publicado en: Cells, 2023, ISSN 2073-4409
Editor: Athanasios Metaxakis
DOI: 10.3390/cells12162024

Non-Invasive Optoacoustic Imaging for In-Depth Cultural Heritage Diagnostics

Autores: George J. Tserevelakis, Eleanna Pirgianaki, Kristalia Melessanaki, Giannis Zacharakis, Costas Fotakis
Publicado en: Photonics, Edición 11, 2024, Página(s) 902, ISSN 2304-6732
Editor: Photonics
DOI: 10.3390/photonics11100902

Spermidine is essential for fasting-mediated autophagy and longevity

Autores: Sebastian J. Hofer, Ioanna Daskalaki, Martina Bergmann, Jasna Friščić, Andreas Zimmermann, Melanie I. Mueller, Mahmoud Abdellatif, Raffaele Nicastro, Sarah Masser, Sylvère Durand, Alexander Nartey, Mara Waltenstorfer, Sarah Enzenhofer, Isabella Faimann, Verena Gschiel, Thomas Bajaj, Christine Niemeyer, Ilias Gkikas, Lukas Pein, Giulia Cerrato, Hui Pan, YongTian Liang, Jelena Tadic, Andrea Jerk
Publicado en: Nature Cell Biology, Edición 26, 2024, Página(s) 1571-1584, ISSN 1465-7392
Editor: Nature Publishing Group
DOI: 10.1038/s41556-024-01468-x

Single-pixel transmission matrix recovery via two-photon fluorescence

Autores: Shupeng Zhao, Bernhard Rauer, Lorenzo Valzania, Jonathan Dong, Ruifeng Liu, Fuli Li, Sylvain Gigan, Hilton B. de Aguiar
Publicado en: Science Advances, Edición 10, 2024, ISSN 2375-2548
Editor: Science
DOI: 10.1126/sciadv.adi3442

Generation of Bessel-like beams with reduced sidelobes for enhanced light-sheet microscopy

Autores: Jerin Geogy George, Kishan Dholakia, and Shanti Bhattacharya
Publicado en: Optics Continuum, 2023, ISSN 2770-0208
Editor: Optica Publishing Group
DOI: 10.1364/optcon.493003

Non-invasive chemically selective energy delivery and focusing inside a scattering medium guided by Raman scattering

Autores: Bingxin Tian, Bernhard Rauer, Antoine Boniface, Jun Han, Sylvain Gigan, Hilton B. de Aguiar
Publicado en: Optics Letters, Edición 47, 2024, Página(s) 2145, ISSN 0146-9592
Editor: Optical Society of America
DOI: 10.1364/ol.456411

Local coordination of mRNA storage and degradation near mitochondria modulates C. elegans ageing

Autores: Ioanna Daskalaki, Maria Markaki, Ilias Gkikas, Nektarios Tavernarakis
Publicado en: The EMBO Journal, 2023, ISSN 1460-2075
Editor: Ioanna Daskalaki
DOI: 10.15252/embj.2022112446

Revealing Underdrawings in Wall Paintings of Complex Stratigraphy with a Novel Reflectance Photoacoustic Imaging Prototype

Autores: Antonina Chaban, George J. Tserevelakis, Evgenia Klironomou, Raffaella Fontana, Giannis Zacharakis, Jana Striova
Publicado en: Journal of Imaging, Edición 7, 2025, Página(s) 250, ISSN 2313-433X
Editor: MDPI
DOI: 10.3390/jimaging7120250

Phase conjugation with spatially incoherent light in complex media

Autores: YoonSeok Baek, Hilton B. de Aguiar & Sylvain Gigan
Publicado en: Nature Photonics, 2023, ISSN 1749-4885
Editor: Nature Pub. Group
DOI: 10.1038/s41566-023-01254-5

Imaging Parhyale hawaiensis embryogenesis with frequency domain photoacoustic microscopy: A novel tool in developmental biology

Autores: George J. Tserevelakis, Sofia Velentza, Ioannis Liaskas, Themis Archontidis, Anastasios Pavlopoulos, Giannis Zacharakis
Publicado en: Journal of Biophtonics, 2022, ISSN 1864-0648
Editor: Wiley-VCH GmbH
DOI: 10.1002/jbio.202200202

Experimentally unsupervised deconvolution for light-sheet microscopy with propagation-invariant beams

Autores: Philip Wijesinghe, Stella Corsetti, Darren J. X. Chow, Shuzo Sakata, Kylie R. Dunning and Kishan Dholakia
Publicado en: Light: Science & Applications, 2022, ISSN 2047-7538
Editor: USTAN
DOI: 10.1038/s41377-022-00975-6

Meshless Monte Carlo radiation transfer method for curved geometries using signed distance functions

Autores: Lewis McMillan, Graham D Bruce, Kishan Dholakia
Publicado en: Journal of Biomedical Optics, 2022, ISSN 1560-2281
Editor: SPIE
DOI: 10.1117/1.jbo.27.8.083003

Roadmap on computational methods in optical imaging and holography [invited]

Autores: Joseph Rosen, Simon Alford, Blake Allan, Vijayakumar Anand, Shlomi Arnon, Francis Gracy Arockiaraj, Jonathan Art, Bijie Bai, Ganesh M. Balasubramaniam, Tobias Birnbaum, Nandan S. Bisht, David Blinder, Liangcai Cao, Qian Chen, Ziyang Chen, Vishesh Dubey, Karen Egiazarian, Mert Ercan, Andrew Forbes, G. Gopakumar, Yunhui Gao, Sylvain Gigan, Paweł Gocłowski, Shivasubramanian Gopinath, Alon Greenbaum
Publicado en: Applied Physics B, Edición 130, 2024, ISSN 0946-2171
Editor: Springer Verlag
DOI: 10.1007/s00340-024-08280-3

UVA Hyperspectral Light-Sheet Microscopy for Volumetric Metabolic Imaging: Application to Preimplantation Embryo Development

Autores: Josephine Morizet, Darren Chow, Philip Wijesinghe, Erik Schartner, George Dwapanyin, Nicolas Dubost, Graham D. Bruce, Ellen Anckaert, Kylie Dunning, Kishan Dholakia
Publicado en: ACS Photonics, Edición 10, 2023, Página(s) 4177-4187, ISSN 2330-4022
Editor: American Chemical Society
DOI: 10.1021/acsphotonics.3c00900

Assessing locomotory rate in response to food for the identification of neuronal and muscular defects in C. elegans

Autores: Dionysia Petratou, Persefoni Fragkiadaki, Eirini Lionaki, Nektarios Tavernarakis
Publicado en: STAR Protocols, Edición 5, 2024, Página(s) 102801, ISSN 2666-1667
Editor: ScienceDirect
DOI: 10.1016/j.xpro.2023.102801

Optoacoustic interferometric characterization system (OPTICS) for the evaluation of fuel quality through speed of sound measurements

Autores: George J. Tserevelakis, Charalampos Astrinakis, Giannis Zacharakis
Publicado en: Ultrasonics, Edición 139, 2024, Página(s) 107291, ISSN 0041-624X
Editor: Elsevier BV
DOI: 10.1016/j.ultras.2024.107291

Age-associated anatomical and physiological alterations in Caenorhabditis elegans

Autores: Emmanuel Spanoudakis, Nektarios Tavernarakis
Publicado en: Mechanisms of Ageing and Development, 2023, ISSN 1872-6216
Editor: Emmanuel Spanoudakis
DOI: 10.1016/j.mad.2023.111827

Remote control of autophagy and metabolism in the liver

Autores: Ilias Gkikas, Ioanna Daskalaki, Konstantinos Kounakis, Nektarios Tavernarakis and Eirini Lionaki
Publicado en: Int. J. Mol. Sci., 2023, ISSN 1422-0067
Editor: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms24044230

Bimodal optical and optoacoustic multiview microscope in the frequency-domain

Autores: Mikis Mylonakis, George J. Tserevelakis, Georgios Vlachos, Eleftheria Fanouraki, Anastasios Pavlopoulos, Michalis Pavlidis, Giannis Zacharakis
Publicado en: Optics Letters, Edición 49, 2024, Página(s) 462, ISSN 0146-9592
Editor: Optical Society of America
DOI: 10.1364/ol.510384

Hybrid Fluorescence and Frequency-Domain Photoacoustic Microscopy for Imaging Development of Parhyale hawaiensis Embryos

Autores: George J. Tserevelakis, Emmanouela Tekonaki, Maria Kalogeridi, Ioannis Liaskas, Anastasios Pavlopoulos and Giannis Zacharakis
Publicado en: Photonics, 2023, ISSN 2304-6732
Editor: George J. Tserevelakis,
DOI: 10.3390/photonics10030264

Revealing Hidden Features in Multilayered Artworks by Means of an Epi-Illumination Photoacoustic Imaging System

Autores: George J. Tserevelakis, Antonina Chaban, Evgenia Klironomou, Kristalia Melessanaki, Jana Striova, Giannis Zacharakis
Publicado en: Journal of Imaging, Edición 7/9, 2021, Página(s) 183, ISSN 2313-433X
Editor: George J. Tserevelakis, Antonina Chaban, Evgenia Klironomou, Kristalia Melessanaki, Jana Striova and Giannis Zacharakis
DOI: 10.3390/jimaging7090183

Hybrid Autofluorescence and Optoacoustic Microscopy for the Label-Free, Early and Rapid Detection of Pathogenic Infections in Vegetative Tissues

Autores: George J. Tserevelakis, Andreas Theocharis, Stavroula Spyropoulou, Emmanouil Trantas, , Dimitrios Goumas, Filippos Ververidis and Giannis Zacharakis
Publicado en: Journal of Imaging, 2023, ISSN 2313-433X
Editor: George J. Tserevelakis
DOI: 10.3390/jimaging9090176

Towards affordable biomedical imaging: Recent advances in low‐cost, high‐resolution optoacoustic microscopy

Autores: Pavlos Kalitsounakis, Giannis Zacharakis, George J. Tserevelakis
Publicado en: Journal of Microscopy, 2024, ISSN 0022-2720
Editor: Blackwell Publishing Inc.
DOI: 10.1111/jmi.13378

Neurophotonics beyond the surface: unmasking the brain’s complexity exploiting optical scattering

Autores: Fei Xia, Caio Vaz Rimoli, Walther Akemann, Cathie Ventalon, Laurent Bourdieu, Sylvain Gigan, Hilton B. de Aguiar
Publicado en: Neurophotonics, Edición 11, 2024, ISSN 2329-423X
Editor: SPIE
DOI: 10.1117/1.nph.11.s1.s11510

Other (1)

Label-free super-resolution chemical imaging of biomedical specimens

Autores: Julien Guilbert, Awoke Negash, Simon Labouesse, Sylvain Gigan, Anne Sentenac, Hilton B. de Aguiar
Publicado en: bioRxiv, 2021
Editor: Cold Spring Harbor Laboratory
DOI: 10.1101/2021.05.14.444185

Derechos de propiedad intelectual

Patent (1)

AN ASSEMBLY OF OPTICAL ELEMENTS AND A METHOD FOR CONTROLLING LIGHT

Número de solicitud/publicación: 22 164624
Fecha: 2022-03-28
Solicitante(s): IDRYMA TECHNOLOGIAS KAI EREVNAS