Dynamic adaptive microscopy for label-free multi-parametric imaging in biology and medicine

Resultado final

Development of initial optimization and transmission matrix calculation algorithms. New concepts to account for refractive index variations with adaptive meshing, Levy statistics and Monte Carlo modelling of light propagation in complex media

Phase retrieval

Development of 3D autocorrelation phase and polarization retrieval algorithms exploiting coherent multiply scattered light in optical, optoacoustic, non-linear and Raman methods

Non-diffracting illumination

Non-diffracting beam formation strategies with appropriate spatial filtering (reconfigurable photonic, biological and biomimetic structures) and coherent structured and speckle illumination

Dissemination and Exploitation plan

Design of our dissemination, exploitation plan and IPR management. Our industrial partners Rayfos and Imagine Optic and the DC have been appointed for coordinating this highly important task

Dissemination and Exploitation interim plan

Implemented actions of our dissemination exploitation plan and IPR management Our industrial partners Rayfos and Imagine Optic and the DC have been appointed for coordinating this highly important task

Proof of concept

Proof of concept imaging and assessment of imaging prototype with freshly excised animal tissue samples retina cancer spheroids

Website/Logo

Design of the project's logo and realization of the dedicated webpage with public sector and protected inranet for partners access only

Data management

Report on the data management plan of the consortium

Publicaciones

Large field-of-view non-invasive imaging through scattering layers using fluctuating random illumination

Autores: Lei Zhu, Fernando Soldevila, , Claudio Moretti, Alexandra d’Arco, Antoine Boniface, Xiaopeng Shao, Hilton B. de Aguiar & Sylvain Gigan
Publicado en: Nature Communications, 2022, ISSN 2041-1723
Editor: Nature Publishing Group
DOI: 10.1038/s41467-022-29166-y

Spatially offset optical coherence tomography: Leveraging multiple scattering for high-contrast imaging at depth in turbid media

Autores: GavrielleR. Untracht, MingzhouChen, PhilipWijesinghe, JosepMas, Harold T. Yura3, DominikMarti, PeterE. Andersen, KishanDholakia
Publicado en: Science Advances, 2023, ISSN 2375-2548
Editor: American Association for the Advancement of Science
DOI: 10.1126/sciadv.adh5435

Hybrid confocal fluorescence and photoacoustic microscopy for the label-free investigation of melanin accumulation in fish scales

Autores: George J. Tserevelakis, Michalis Pavlidis, Athanasios Samaras, Georgios D. Barmparis, Kostas G. Mavrakis, Ioannis Draganidis, Athanasios Oikonomou, Eleftheria Fanouraki, Giorgos P. Tsironis & Giannis Zacharakis
Publicado en: Scientific Reports, Edición Edición 12, 2022, ISSN 2045-2322
Editor: Nature Publishing Group
DOI: 10.1038/s41598-022-11262-0

Stratigraphy of Fresco Paintings: A New Approach with Photoacoustic and SORS Imaging

Autores: Francesca A. Pisu , Daniele Chiriu Evgenia Klironomou, Giannis Zacharakis and George J. Tserevelakis
Publicado en: Journal of Imaging, 2023, ISSN 2313-433X
Editor: Francesca A. Pisu
DOI: 10.3390/jimaging9010016

A Cost-Efficient Multiwavelength LED-Based System for Quantitative Photoacoustic Measurements

Autores: Michalis Orfanakis, George J. Tserevelakis, Giannis Zacharakis
Publicado en: Sensors, Edición 21/14, 2021, Página(s) 4888, ISSN 1424-8220
Editor: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/s21144888

A proton-inhibited DEG/ENaC ion channel maintains neuronal ionstasis and promotes neuronal survival under stress

Autores: Dionysia Petratou, Martha Gjikolaj, Eva Kaulich, William Schafer, Nektarios Tavernarakis
Publicado en: iScience, 2023, ISSN 2589-0042
Editor: Dionysia Petratou
DOI: 10.1016/j.isci.2023.107117

To focus-match or not to focus-match inverse spatially offset Raman spectroscopy: a question of light penetration

Autores: Georgina E. Shillito, Lewis Mcmillan, Graham D. Bruce, and Kishan Dholakia
Publicado en: Optics Express, 2022, ISSN 1094-4087
Editor: Optical Society of America
DOI: 10.1364/oe.451496

High precision photoacoustic interferometer for the determination of the speed of sound in liquid media

Autores: George J. Tserevelakis and Giannis Zacharakis
Publicado en: Optics Express, 2022
Editor: George J. Tserevelakis
DOI: 10.1364/oe.455557

Agar Gel as a Non-Invasive Coupling Medium for Reflectance Photoacoustic (PA) Imaging: Experimental Results on Wall-Painting Mock-Ups

Autores: Antonina Chaban , George J. Tserevelakis, Evgenia Klironomou, Giannis Zacharakis and Jana Striov
Publicado en: Journal of Imaging, 2022, ISSN 2313-433X
Editor: Antonina Chaban
DOI: 10.3390/jimaging8090235

Full image reconstruction in frequency-domain photoacoustic microscopy by means of a low-cost I/Q demodulator

Autores: George J. Tserevelakis, Kostas G. Mavrakis, Nikitas Kakakios, Giannis Zacharakis
Publicado en: Optics Letters, Edición 46/19, 2021, Página(s) 4718, ISSN 0146-9592
Editor: Optical Society of America
DOI: 10.1364/ol.435146

PML Differentially Regulates Growth and Invasion in Brain Cancer.

Autores: Maria Tampakaki; Maria Tampakaki; Mariam-Eleni Oraiopoulou; Eleftheria Tzamali; Giorgos Tzedakis; Takis Makatounakis; Giannis Zacharakis; Joseph Papamatheakis; Joseph Papamatheakis; Vangelis Sakkalis
Publicado en: Edición 12, Edición 11, 2021, ISSN 1422-0067
Editor: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms22126289

Neuronal atg1 Coordinates Autophagy Induction and Physiological Adaptations to Balance mTORC1 Signalling

Autores: Athanasios Metaxakis Michail Pavlidis and Nektarios Tavernarakis
Publicado en: Cells, 2023, ISSN 2073-4409
Editor: Athanasios Metaxakis
DOI: 10.3390/cells12162024

Generation of Bessel-like beams with reduced sidelobes for enhanced light-sheet microscopy

Autores: Jerin Geogy George, Kishan Dholakia, and Shanti Bhattacharya
Publicado en: Optics Continuum, 2023, ISSN 2770-0208
Editor: Optica Publishing Group
DOI: 10.1364/optcon.493003

Local coordination of mRNA storage and degradation near mitochondria modulates C. elegans ageing

Autores: Ioanna Daskalaki, Maria Markaki, Ilias Gkikas, Nektarios Tavernarakis
Publicado en: The EMBO Journal, 2023, ISSN 1460-2075
Editor: Ioanna Daskalaki
DOI: 10.15252/embj.2022112446

Phase conjugation with spatially incoherent light in complex media

Autores: YoonSeok Baek, Hilton B. de Aguiar & Sylvain Gigan
Publicado en: Nature Photonics, 2023, ISSN 1749-4885
Editor: Nature Pub. Group
DOI: 10.1038/s41566-023-01254-5

Imaging Parhyale hawaiensis embryogenesis with frequency domain photoacoustic microscopy: A novel tool in developmental biology

Autores: George J. Tserevelakis, Sofia Velentza, Ioannis Liaskas, Themis Archontidis, Anastasios Pavlopoulos, Giannis Zacharakis
Publicado en: Journal of Biophtonics, 2022, ISSN 1864-0648
Editor: Wiley-VCH GmbH
DOI: 10.1002/jbio.202200202

Experimentally unsupervised deconvolution for light-sheet microscopy with propagation-invariant beams

Autores: Philip Wijesinghe, Stella Corsetti, Darren J. X. Chow, Shuzo Sakata, Kylie R. Dunning and Kishan Dholakia
Publicado en: Light: Science & Applications, 2022, ISSN 2047-7538
Editor: USTAN
DOI: 10.1038/s41377-022-00975-6

Meshless Monte Carlo radiation transfer method for curved geometries using signed distance functions

Autores: Lewis McMillan, Graham D Bruce, Kishan Dholakia
Publicado en: Journal of Biomedical Optics, 2022, ISSN 1560-2281
Editor: SPIE
DOI: 10.1117/1.jbo.27.8.083003

Age-associated anatomical and physiological alterations in Caenorhabditis elegans

Autores: Emmanuel Spanoudakis, Nektarios Tavernarakis
Publicado en: Mechanisms of Ageing and Development, 2023, ISSN 1872-6216
Editor: Emmanuel Spanoudakis
DOI: 10.1016/j.mad.2023.111827

Remote control of autophagy and metabolism in the liver

Autores: Ilias Gkikas, Ioanna Daskalaki, Konstantinos Kounakis, Nektarios Tavernarakis and Eirini Lionaki
Publicado en: Int. J. Mol. Sci., 2023, ISSN 1422-0067
Editor: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms24044230

Hybrid Fluorescence and Frequency-Domain Photoacoustic Microscopy for Imaging Development of Parhyale hawaiensis Embryos

Autores: George J. Tserevelakis, Emmanouela Tekonaki, Maria Kalogeridi, Ioannis Liaskas, Anastasios Pavlopoulos and Giannis Zacharakis
Publicado en: Photonics, 2023, ISSN 2304-6732
Editor: George J. Tserevelakis,
DOI: 10.3390/photonics10030264

Revealing Hidden Features in Multilayered Artworks by Means of an Epi-Illumination Photoacoustic Imaging System

Autores: George J. Tserevelakis, Antonina Chaban, Evgenia Klironomou, Kristalia Melessanaki, Jana Striova, Giannis Zacharakis
Publicado en: Journal of Imaging, Edición 7/9, 2021, Página(s) 183, ISSN 2313-433X
Editor: George J. Tserevelakis, Antonina Chaban, Evgenia Klironomou, Kristalia Melessanaki, Jana Striova and Giannis Zacharakis
DOI: 10.3390/jimaging7090183

Hybrid Autofluorescence and Optoacoustic Microscopy for the Label-Free, Early and Rapid Detection of Pathogenic Infections in Vegetative Tissues

Autores: George J. Tserevelakis, Andreas Theocharis, Stavroula Spyropoulou, Emmanouil Trantas, , Dimitrios Goumas, Filippos Ververidis and Giannis Zacharakis
Publicado en: Journal of Imaging, 2023, ISSN 2313-433X
Editor: George J. Tserevelakis
DOI: 10.3390/jimaging9090176

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