Dynamic adaptive microscopy for label-free multi-parametric imaging in biology and medicine

Informations projet

DynAMic

N° de convention de subvention: 863203

DOI 10.3030/863203

Date de signature de la CE 2 Septembre 2019

Date de début 1 Janvier 2020

Date de fin 31 Decembre 2024

Financé au titre de

• EXCELLENT SCIENCE - Future and Emerging Technologies (FET)

Coût total € 3 957 137,50

Contribution de l’UE € 3 957 137,50

Coordonné par

IDRYMA TECHNOLOGIAS KAI EREVNAS
Greece

Livrables

Demonstrators, pilots, prototypes (3)

Optimization algorithms

Development of initial optimization and transmission matrix calculation algorithms. New concepts to account for refractive index variations with adaptive meshing, Levy statistics and Monte Carlo modelling of light propagation in complex media

Phase retrieval

Development of 3D autocorrelation phase and polarization retrieval algorithms exploiting coherent multiply scattered light in optical, optoacoustic, non-linear and Raman methods

Non-diffracting illumination

Non-diffracting beam formation strategies with appropriate spatial filtering (reconfigurable photonic, biological and biomimetic structures) and coherent structured and speckle illumination

Documents, reports (3)

Dissemination and Exploitation plan

Design of our dissemination, exploitation plan and IPR management. Our industrial partners Rayfos and Imagine Optic and the DC have been appointed for coordinating this highly important task

Dissemination and Exploitation interim plan

Implemented actions of our dissemination exploitation plan and IPR management Our industrial partners Rayfos and Imagine Optic and the DC have been appointed for coordinating this highly important task

Proof of concept

Proof of concept imaging and assessment of imaging prototype with freshly excised animal tissue samples retina cancer spheroids

Websites, patent fillings, videos etc. (1)

Website/Logo

Design of the project's logo and realization of the dedicated webpage with public sector and protected inranet for partners access only

Open Research Data Pilot (1)

Data management

Report on the data management plan of the consortium

Publications

Peer reviewed articles (23)

Large field-of-view non-invasive imaging through scattering layers using fluctuating random illumination

Auteurs: Lei Zhu, Fernando Soldevila, , Claudio Moretti, Alexandra d’Arco, Antoine Boniface, Xiaopeng Shao, Hilton B. de Aguiar & Sylvain Gigan
Publié dans: Nature Communications, 2022, ISSN 2041-1723
Éditeur: Nature Publishing Group
DOI: 10.1038/s41467-022-29166-y

Spatially offset optical coherence tomography: Leveraging multiple scattering for high-contrast imaging at depth in turbid media

Auteurs: GavrielleR. Untracht, MingzhouChen, PhilipWijesinghe, JosepMas, Harold T. Yura3, DominikMarti, PeterE. Andersen, KishanDholakia
Publié dans: Science Advances, 2023, ISSN 2375-2548
Éditeur: American Association for the Advancement of Science
DOI: 10.1126/sciadv.adh5435

Hybrid confocal fluorescence and photoacoustic microscopy for the label-free investigation of melanin accumulation in fish scales

Auteurs: George J. Tserevelakis, Michalis Pavlidis, Athanasios Samaras, Georgios D. Barmparis, Kostas G. Mavrakis, Ioannis Draganidis, Athanasios Oikonomou, Eleftheria Fanouraki, Giorgos P. Tsironis & Giannis Zacharakis
Publié dans: Scientific Reports, Numéro Numéro 12, 2022, ISSN 2045-2322
Éditeur: Nature Publishing Group
DOI: 10.1038/s41598-022-11262-0

Stratigraphy of Fresco Paintings: A New Approach with Photoacoustic and SORS Imaging

Auteurs: Francesca A. Pisu , Daniele Chiriu Evgenia Klironomou, Giannis Zacharakis and George J. Tserevelakis
Publié dans: Journal of Imaging, 2023, ISSN 2313-433X
Éditeur: Francesca A. Pisu
DOI: 10.3390/jimaging9010016

A Cost-Efficient Multiwavelength LED-Based System for Quantitative Photoacoustic Measurements

Auteurs: Michalis Orfanakis, George J. Tserevelakis, Giannis Zacharakis
Publié dans: Sensors, Numéro 21/14, 2021, Page(s) 4888, ISSN 1424-8220
Éditeur: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/s21144888

A proton-inhibited DEG/ENaC ion channel maintains neuronal ionstasis and promotes neuronal survival under stress

Auteurs: Dionysia Petratou, Martha Gjikolaj, Eva Kaulich, William Schafer, Nektarios Tavernarakis
Publié dans: iScience, 2023, ISSN 2589-0042
Éditeur: Dionysia Petratou
DOI: 10.1016/j.isci.2023.107117

To focus-match or not to focus-match inverse spatially offset Raman spectroscopy: a question of light penetration

Auteurs: Georgina E. Shillito, Lewis Mcmillan, Graham D. Bruce, and Kishan Dholakia
Publié dans: Optics Express, 2022, ISSN 1094-4087
Éditeur: Optical Society of America
DOI: 10.1364/oe.451496

High precision photoacoustic interferometer for the determination of the speed of sound in liquid media

Auteurs: George J. Tserevelakis and Giannis Zacharakis
Publié dans: Optics Express, 2022
Éditeur: George J. Tserevelakis
DOI: 10.1364/oe.455557

Agar Gel as a Non-Invasive Coupling Medium for Reflectance Photoacoustic (PA) Imaging: Experimental Results on Wall-Painting Mock-Ups

Auteurs: Antonina Chaban , George J. Tserevelakis, Evgenia Klironomou, Giannis Zacharakis and Jana Striov
Publié dans: Journal of Imaging, 2022, ISSN 2313-433X
Éditeur: Antonina Chaban
DOI: 10.3390/jimaging8090235

Full image reconstruction in frequency-domain photoacoustic microscopy by means of a low-cost I/Q demodulator

Auteurs: George J. Tserevelakis, Kostas G. Mavrakis, Nikitas Kakakios, Giannis Zacharakis
Publié dans: Optics Letters, Numéro 46/19, 2021, Page(s) 4718, ISSN 0146-9592
Éditeur: Optical Society of America
DOI: 10.1364/ol.435146

PML Differentially Regulates Growth and Invasion in Brain Cancer.

Auteurs: Maria Tampakaki; Maria Tampakaki; Mariam-Eleni Oraiopoulou; Eleftheria Tzamali; Giorgos Tzedakis; Takis Makatounakis; Giannis Zacharakis; Joseph Papamatheakis; Joseph Papamatheakis; Vangelis Sakkalis
Publié dans: Numéro 12, Numéro 11, 2021, ISSN 1422-0067
Éditeur: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms22126289

Neuronal atg1 Coordinates Autophagy Induction and Physiological Adaptations to Balance mTORC1 Signalling

Auteurs: Athanasios Metaxakis Michail Pavlidis and Nektarios Tavernarakis
Publié dans: Cells, 2023, ISSN 2073-4409
Éditeur: Athanasios Metaxakis
DOI: 10.3390/cells12162024

Generation of Bessel-like beams with reduced sidelobes for enhanced light-sheet microscopy

Auteurs: Jerin Geogy George, Kishan Dholakia, and Shanti Bhattacharya
Publié dans: Optics Continuum, 2023, ISSN 2770-0208
Éditeur: Optica Publishing Group
DOI: 10.1364/optcon.493003

Local coordination of mRNA storage and degradation near mitochondria modulates C. elegans ageing

Auteurs: Ioanna Daskalaki, Maria Markaki, Ilias Gkikas, Nektarios Tavernarakis
Publié dans: The EMBO Journal, 2023, ISSN 1460-2075
Éditeur: Ioanna Daskalaki
DOI: 10.15252/embj.2022112446

Phase conjugation with spatially incoherent light in complex media

Auteurs: YoonSeok Baek, Hilton B. de Aguiar & Sylvain Gigan
Publié dans: Nature Photonics, 2023, ISSN 1749-4885
Éditeur: Nature Pub. Group
DOI: 10.1038/s41566-023-01254-5

Imaging Parhyale hawaiensis embryogenesis with frequency domain photoacoustic microscopy: A novel tool in developmental biology

Auteurs: George J. Tserevelakis, Sofia Velentza, Ioannis Liaskas, Themis Archontidis, Anastasios Pavlopoulos, Giannis Zacharakis
Publié dans: Journal of Biophtonics, 2022, ISSN 1864-0648
Éditeur: Wiley-VCH GmbH
DOI: 10.1002/jbio.202200202

Experimentally unsupervised deconvolution for light-sheet microscopy with propagation-invariant beams

Auteurs: Philip Wijesinghe, Stella Corsetti, Darren J. X. Chow, Shuzo Sakata, Kylie R. Dunning and Kishan Dholakia
Publié dans: Light: Science & Applications, 2022, ISSN 2047-7538
Éditeur: USTAN
DOI: 10.1038/s41377-022-00975-6

Meshless Monte Carlo radiation transfer method for curved geometries using signed distance functions

Auteurs: Lewis McMillan, Graham D Bruce, Kishan Dholakia
Publié dans: Journal of Biomedical Optics, 2022, ISSN 1560-2281
Éditeur: SPIE
DOI: 10.1117/1.jbo.27.8.083003

Age-associated anatomical and physiological alterations in Caenorhabditis elegans

Auteurs: Emmanuel Spanoudakis, Nektarios Tavernarakis
Publié dans: Mechanisms of Ageing and Development, 2023, ISSN 1872-6216
Éditeur: Emmanuel Spanoudakis
DOI: 10.1016/j.mad.2023.111827

Remote control of autophagy and metabolism in the liver

Auteurs: Ilias Gkikas, Ioanna Daskalaki, Konstantinos Kounakis, Nektarios Tavernarakis and Eirini Lionaki
Publié dans: Int. J. Mol. Sci., 2023, ISSN 1422-0067
Éditeur: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms24044230

Hybrid Fluorescence and Frequency-Domain Photoacoustic Microscopy for Imaging Development of Parhyale hawaiensis Embryos

Auteurs: George J. Tserevelakis, Emmanouela Tekonaki, Maria Kalogeridi, Ioannis Liaskas, Anastasios Pavlopoulos and Giannis Zacharakis
Publié dans: Photonics, 2023, ISSN 2304-6732
Éditeur: George J. Tserevelakis,
DOI: 10.3390/photonics10030264

Revealing Hidden Features in Multilayered Artworks by Means of an Epi-Illumination Photoacoustic Imaging System

Auteurs: George J. Tserevelakis, Antonina Chaban, Evgenia Klironomou, Kristalia Melessanaki, Jana Striova, Giannis Zacharakis
Publié dans: Journal of Imaging, Numéro 7/9, 2021, Page(s) 183, ISSN 2313-433X
Éditeur: George J. Tserevelakis, Antonina Chaban, Evgenia Klironomou, Kristalia Melessanaki, Jana Striova and Giannis Zacharakis
DOI: 10.3390/jimaging7090183

Hybrid Autofluorescence and Optoacoustic Microscopy for the Label-Free, Early and Rapid Detection of Pathogenic Infections in Vegetative Tissues

Auteurs: George J. Tserevelakis, Andreas Theocharis, Stavroula Spyropoulou, Emmanouil Trantas, , Dimitrios Goumas, Filippos Ververidis and Giannis Zacharakis
Publié dans: Journal of Imaging, 2023, ISSN 2313-433X
Éditeur: George J. Tserevelakis
DOI: 10.3390/jimaging9090176