Dynamic adaptive microscopy for label-free multi-parametric imaging in biology and medicine

Informazioni relative al progetto

DynAMic

ID dell’accordo di sovvenzione: 863203

DOI 10.3030/863203

Data della firma CE 2 Settembre 2019

Data di avvio 1 Gennaio 2020

Data di completamento 31 Dicembre 2024

Finanziato da

• EXCELLENT SCIENCE - Future and Emerging Technologies (FET)

Costo totale € 3 957 137,50

Contributo UE € 3 957 137,50

Coordinato da

IDRYMA TECHNOLOGIAS KAI EREVNAS
Greece

Risultati finali

Demonstrators, pilots, prototypes (3)

Optimization algorithms

Development of initial optimization and transmission matrix calculation algorithms. New concepts to account for refractive index variations with adaptive meshing, Levy statistics and Monte Carlo modelling of light propagation in complex media

Phase retrieval

Development of 3D autocorrelation phase and polarization retrieval algorithms exploiting coherent multiply scattered light in optical, optoacoustic, non-linear and Raman methods

Non-diffracting illumination

Non-diffracting beam formation strategies with appropriate spatial filtering (reconfigurable photonic, biological and biomimetic structures) and coherent structured and speckle illumination

Documents, reports (3)

Dissemination and Exploitation plan

Design of our dissemination, exploitation plan and IPR management. Our industrial partners Rayfos and Imagine Optic and the DC have been appointed for coordinating this highly important task

Dissemination and Exploitation interim plan

Implemented actions of our dissemination exploitation plan and IPR management Our industrial partners Rayfos and Imagine Optic and the DC have been appointed for coordinating this highly important task

Proof of concept

Proof of concept imaging and assessment of imaging prototype with freshly excised animal tissue samples retina cancer spheroids

Websites, patent fillings, videos etc. (1)

Website/Logo

Design of the project's logo and realization of the dedicated webpage with public sector and protected inranet for partners access only

Open Research Data Pilot (1)

Data management

Report on the data management plan of the consortium

Pubblicazioni

Peer reviewed articles (23)

Large field-of-view non-invasive imaging through scattering layers using fluctuating random illumination

Autori: Lei Zhu, Fernando Soldevila, , Claudio Moretti, Alexandra d’Arco, Antoine Boniface, Xiaopeng Shao, Hilton B. de Aguiar & Sylvain Gigan
Pubblicato in: Nature Communications, 2022, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-022-29166-y

Spatially offset optical coherence tomography: Leveraging multiple scattering for high-contrast imaging at depth in turbid media

Autori: GavrielleR. Untracht, MingzhouChen, PhilipWijesinghe, JosepMas, Harold T. Yura3, DominikMarti, PeterE. Andersen, KishanDholakia
Pubblicato in: Science Advances, 2023, ISSN 2375-2548
Editore: American Association for the Advancement of Science
DOI: 10.1126/sciadv.adh5435

Hybrid confocal fluorescence and photoacoustic microscopy for the label-free investigation of melanin accumulation in fish scales

Autori: George J. Tserevelakis, Michalis Pavlidis, Athanasios Samaras, Georgios D. Barmparis, Kostas G. Mavrakis, Ioannis Draganidis, Athanasios Oikonomou, Eleftheria Fanouraki, Giorgos P. Tsironis & Giannis Zacharakis
Pubblicato in: Scientific Reports, Numero Numero 12, 2022, ISSN 2045-2322
Editore: Nature Publishing Group
DOI: 10.1038/s41598-022-11262-0

Stratigraphy of Fresco Paintings: A New Approach with Photoacoustic and SORS Imaging

Autori: Francesca A. Pisu , Daniele Chiriu Evgenia Klironomou, Giannis Zacharakis and George J. Tserevelakis
Pubblicato in: Journal of Imaging, 2023, ISSN 2313-433X
Editore: Francesca A. Pisu
DOI: 10.3390/jimaging9010016

A Cost-Efficient Multiwavelength LED-Based System for Quantitative Photoacoustic Measurements

Autori: Michalis Orfanakis, George J. Tserevelakis, Giannis Zacharakis
Pubblicato in: Sensors, Numero 21/14, 2021, Pagina/e 4888, ISSN 1424-8220
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/s21144888

A proton-inhibited DEG/ENaC ion channel maintains neuronal ionstasis and promotes neuronal survival under stress

Autori: Dionysia Petratou, Martha Gjikolaj, Eva Kaulich, William Schafer, Nektarios Tavernarakis
Pubblicato in: iScience, 2023, ISSN 2589-0042
Editore: Dionysia Petratou
DOI: 10.1016/j.isci.2023.107117

To focus-match or not to focus-match inverse spatially offset Raman spectroscopy: a question of light penetration

Autori: Georgina E. Shillito, Lewis Mcmillan, Graham D. Bruce, and Kishan Dholakia
Pubblicato in: Optics Express, 2022, ISSN 1094-4087
Editore: Optical Society of America
DOI: 10.1364/oe.451496

High precision photoacoustic interferometer for the determination of the speed of sound in liquid media

Autori: George J. Tserevelakis and Giannis Zacharakis
Pubblicato in: Optics Express, 2022
Editore: George J. Tserevelakis
DOI: 10.1364/oe.455557

Agar Gel as a Non-Invasive Coupling Medium for Reflectance Photoacoustic (PA) Imaging: Experimental Results on Wall-Painting Mock-Ups

Autori: Antonina Chaban , George J. Tserevelakis, Evgenia Klironomou, Giannis Zacharakis and Jana Striov
Pubblicato in: Journal of Imaging, 2022, ISSN 2313-433X
Editore: Antonina Chaban
DOI: 10.3390/jimaging8090235

Full image reconstruction in frequency-domain photoacoustic microscopy by means of a low-cost I/Q demodulator

Autori: George J. Tserevelakis, Kostas G. Mavrakis, Nikitas Kakakios, Giannis Zacharakis
Pubblicato in: Optics Letters, Numero 46/19, 2021, Pagina/e 4718, ISSN 0146-9592
Editore: Optical Society of America
DOI: 10.1364/ol.435146

PML Differentially Regulates Growth and Invasion in Brain Cancer.

Autori: Maria Tampakaki; Maria Tampakaki; Mariam-Eleni Oraiopoulou; Eleftheria Tzamali; Giorgos Tzedakis; Takis Makatounakis; Giannis Zacharakis; Joseph Papamatheakis; Joseph Papamatheakis; Vangelis Sakkalis
Pubblicato in: Numero 12, Numero 11, 2021, ISSN 1422-0067
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms22126289

Neuronal atg1 Coordinates Autophagy Induction and Physiological Adaptations to Balance mTORC1 Signalling

Autori: Athanasios Metaxakis Michail Pavlidis and Nektarios Tavernarakis
Pubblicato in: Cells, 2023, ISSN 2073-4409
Editore: Athanasios Metaxakis
DOI: 10.3390/cells12162024

Generation of Bessel-like beams with reduced sidelobes for enhanced light-sheet microscopy

Autori: Jerin Geogy George, Kishan Dholakia, and Shanti Bhattacharya
Pubblicato in: Optics Continuum, 2023, ISSN 2770-0208
Editore: Optica Publishing Group
DOI: 10.1364/optcon.493003

Local coordination of mRNA storage and degradation near mitochondria modulates C. elegans ageing

Autori: Ioanna Daskalaki, Maria Markaki, Ilias Gkikas, Nektarios Tavernarakis
Pubblicato in: The EMBO Journal, 2023, ISSN 1460-2075
Editore: Ioanna Daskalaki
DOI: 10.15252/embj.2022112446

Phase conjugation with spatially incoherent light in complex media

Autori: YoonSeok Baek, Hilton B. de Aguiar & Sylvain Gigan
Pubblicato in: Nature Photonics, 2023, ISSN 1749-4885
Editore: Nature Pub. Group
DOI: 10.1038/s41566-023-01254-5

Imaging Parhyale hawaiensis embryogenesis with frequency domain photoacoustic microscopy: A novel tool in developmental biology

Autori: George J. Tserevelakis, Sofia Velentza, Ioannis Liaskas, Themis Archontidis, Anastasios Pavlopoulos, Giannis Zacharakis
Pubblicato in: Journal of Biophtonics, 2022, ISSN 1864-0648
Editore: Wiley-VCH GmbH
DOI: 10.1002/jbio.202200202

Experimentally unsupervised deconvolution for light-sheet microscopy with propagation-invariant beams

Autori: Philip Wijesinghe, Stella Corsetti, Darren J. X. Chow, Shuzo Sakata, Kylie R. Dunning and Kishan Dholakia
Pubblicato in: Light: Science & Applications, 2022, ISSN 2047-7538
Editore: USTAN
DOI: 10.1038/s41377-022-00975-6

Meshless Monte Carlo radiation transfer method for curved geometries using signed distance functions

Autori: Lewis McMillan, Graham D Bruce, Kishan Dholakia
Pubblicato in: Journal of Biomedical Optics, 2022, ISSN 1560-2281
Editore: SPIE
DOI: 10.1117/1.jbo.27.8.083003

Age-associated anatomical and physiological alterations in Caenorhabditis elegans

Autori: Emmanuel Spanoudakis, Nektarios Tavernarakis
Pubblicato in: Mechanisms of Ageing and Development, 2023, ISSN 1872-6216
Editore: Emmanuel Spanoudakis
DOI: 10.1016/j.mad.2023.111827

Remote control of autophagy and metabolism in the liver

Autori: Ilias Gkikas, Ioanna Daskalaki, Konstantinos Kounakis, Nektarios Tavernarakis and Eirini Lionaki
Pubblicato in: Int. J. Mol. Sci., 2023, ISSN 1422-0067
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms24044230

Hybrid Fluorescence and Frequency-Domain Photoacoustic Microscopy for Imaging Development of Parhyale hawaiensis Embryos

Autori: George J. Tserevelakis, Emmanouela Tekonaki, Maria Kalogeridi, Ioannis Liaskas, Anastasios Pavlopoulos and Giannis Zacharakis
Pubblicato in: Photonics, 2023, ISSN 2304-6732
Editore: George J. Tserevelakis,
DOI: 10.3390/photonics10030264

Revealing Hidden Features in Multilayered Artworks by Means of an Epi-Illumination Photoacoustic Imaging System

Autori: George J. Tserevelakis, Antonina Chaban, Evgenia Klironomou, Kristalia Melessanaki, Jana Striova, Giannis Zacharakis
Pubblicato in: Journal of Imaging, Numero 7/9, 2021, Pagina/e 183, ISSN 2313-433X
Editore: George J. Tserevelakis, Antonina Chaban, Evgenia Klironomou, Kristalia Melessanaki, Jana Striova and Giannis Zacharakis
DOI: 10.3390/jimaging7090183

Hybrid Autofluorescence and Optoacoustic Microscopy for the Label-Free, Early and Rapid Detection of Pathogenic Infections in Vegetative Tissues

Autori: George J. Tserevelakis, Andreas Theocharis, Stavroula Spyropoulou, Emmanouil Trantas, , Dimitrios Goumas, Filippos Ververidis and Giannis Zacharakis
Pubblicato in: Journal of Imaging, 2023, ISSN 2313-433X
Editore: George J. Tserevelakis
DOI: 10.3390/jimaging9090176