Dynamic adaptive microscopy for label-free multi-parametric imaging in biology and medicine

Informazioni relative al progetto

DynAMic

ID dell’accordo di sovvenzione: 863203

DOI

10.3030/863203

Progetto chiuso

Data della firma CE 2 Settembre 2019

Data di avvio 1 Gennaio 2020

Data di completamento 31 Dicembre 2024

Finanziato da

• EXCELLENT SCIENCE - Future and Emerging Technologies (FET)

Costo totale

€ 3 957 137,50

Contributo UE

€ 3 957 137,50

Coordinato da

IDRYMA TECHNOLOGIAS KAI EREVNAS
Greece

CORDIS fornisce collegamenti ai risultati finali pubblici e alle pubblicazioni dei progetti ORIZZONTE.

I link ai risultati e alle pubblicazioni dei progetti del 7° PQ, così come i link ad alcuni tipi di risultati specifici come dataset e software, sono recuperati dinamicamente da .OpenAIRE .

Risultati finali

Demonstrators, pilots, prototypes (7)

Optimization algorithms

Development of initial optimization and transmission matrix calculation algorithms. New concepts to account for refractive index variations with adaptive meshing, Levy statistics and Monte Carlo modelling of light propagation in complex media

Phase retrieval

Development of 3D autocorrelation phase and polarization retrieval algorithms exploiting coherent multiply scattered light in optical, optoacoustic, non-linear and Raman methods

Non-diffracting illumination

Non-diffracting beam formation strategies with appropriate spatial filtering (reconfigurable photonic, biological and biomimetic structures) and coherent structured and speckle illumination

Adaptive module

Combine ultrafast wavefront shaping adaptive optomechatronics technologies (build in WP3 and WP4) in one microscopy enabled module

SRS full integration

Build coherent stimulated Raman Scattering (SRS) ability for fast identification of molecular fingerprints (identified in Task 6.2)

Integrated system

Integrate the different technologies and methods in a single prototype microscopy system, optimizing its operational characteristics in terms of sensitivity and specificity and identify safety appropriateness for animal and human use

Molecular/Proteomic fingerprints

Steer morphological & molecular fingerprinting (Raman) towards clinically related targets / questions by adapted fast Stimulated Raman Scattering (SRS) against the identified proteomic biomarkers (built in Task 5.3)

Documents, reports (6)

Raman analysis

Using a conventional Raman imaging system to analyse spectral signatures in relation to proteomic knowledge and identify molecules/proteins of clinical interest

In vivo assessment

Assess in vivo imaging ability of system in animals (rat, rabbit and pig; years 3, 4) and clinical relevance of new techniques and markers derived thereof in comparison with established clinical procedures and diagnostic markers

Dissemination and Exploitation final plan

Implemented actions of our dissemination, exploitation plan, and IPR management. Our industrial partners Rayfos and Imagine Optic and the DC have been appointed for coordinating this highly important task

Dissemination and Exploitation plan

Design of our dissemination, exploitation plan and IPR management. Our industrial partners Rayfos and Imagine Optic and the DC have been appointed for coordinating this highly important task

Dissemination and Exploitation interim plan

Implemented actions of our dissemination exploitation plan and IPR management Our industrial partners Rayfos and Imagine Optic and the DC have been appointed for coordinating this highly important task

Proof of concept

Proof of concept imaging and assessment of imaging prototype with freshly excised animal tissue samples retina cancer spheroids

Other (1)

Summer school

FORTH will organize a summer school on Biophotonics in Life Sciences, taking place on month 24. All involved parties will participate and leading scientists will be invited to teach the current state of the art as well as complimentary skills (IP management, entrepreneurship, proposal writing etc).

Websites, patent fillings, videos etc. (1)

Website/Logo

Design of the project's logo and realization of the dedicated webpage with public sector and protected inranet for partners access only

Open Research Data Pilot (1)

Data management

Report on the data management plan of the consortium

Pubblicazioni

Peer reviewed articles (45)

Large field-of-view non-invasive imaging through scattering layers using fluctuating random illumination

Autori: Lei Zhu, Fernando Soldevila, , Claudio Moretti, Alexandra d’Arco, Antoine Boniface, Xiaopeng Shao, Hilton B. de Aguiar & Sylvain Gigan
Pubblicato in: Nature Communications, 2022, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-022-29166-y

Spatially offset optical coherence tomography: Leveraging multiple scattering for high-contrast imaging at depth in turbid media

Autori: GavrielleR. Untracht, MingzhouChen, PhilipWijesinghe, JosepMas, Harold T. Yura3, DominikMarti, PeterE. Andersen, KishanDholakia
Pubblicato in: Science Advances, 2023, ISSN 2375-2548
Editore: American Association for the Advancement of Science
DOI: 10.1126/sciadv.adh5435

Hybrid confocal fluorescence and photoacoustic microscopy for the label-free investigation of melanin accumulation in fish scales

Autori: George J. Tserevelakis, Michalis Pavlidis, Athanasios Samaras, Georgios D. Barmparis, Kostas G. Mavrakis, Ioannis Draganidis, Athanasios Oikonomou, Eleftheria Fanouraki, Giorgos P. Tsironis & Giannis Zacharakis
Pubblicato in: Scientific Reports, Numero Numero 12, 2022, ISSN 2045-2322
Editore: Nature Publishing Group
DOI: 10.1038/s41598-022-11262-0

Stratigraphy of Fresco Paintings: A New Approach with Photoacoustic and SORS Imaging

Autori: Francesca A. Pisu , Daniele Chiriu Evgenia Klironomou, Giannis Zacharakis and George J. Tserevelakis
Pubblicato in: Journal of Imaging, 2023, ISSN 2313-433X
Editore: Francesca A. Pisu
DOI: 10.3390/jimaging9010016

Sidelobe suppressed Bessel beams for one-photon light-sheet microscopy

Autori: Chetna Taneja, Jerin Geogy George, Stella Corsetti, Philip Wijesinghe, Graham D. Bruce, Maarten F. Zwart, Shanti Bhattacharya, Kishan Dholakia
Pubblicato in: Biomedical Optics Express, Numero 15, 2024, Pagina/e 6183, ISSN 2156-7085
Editore: The Optical Society
DOI: 10.1364/boe.538253

Complement Factor H Loss in RPE Cells Causes Retinal Degeneration in a Human RPE-Porcine Retinal Explant Co-Culture Model

Autori: Angela Armento, Aparna Murali, Julia Marzi, Ana C Almansa-Garcia, Blanca Arango-Gonzalez, Ellen Kilger, Simon J Clark, Katja Schenke-Layland, Charmaine A Ramlogan-Steel, Jason C Steel, Marius Ueffing
Pubblicato in: Biomolecules, Numero 11, 2024, Pagina/e 1621, ISSN 2218-273X
Editore: MDPI
DOI: 10.3390/biom11111621

Online learning of the transmission matrix of dynamic scattering media

Autori: Lorenzo Valzania, Sylvain Gigan
Pubblicato in: Optica, Numero 10, 2024, Pagina/e 708, ISSN 2334-2536
Editore: Optica
DOI: 10.1364/optica.479962

Optically generated droplet beams improve optoacoustic imaging of choroid thickness as an Alzheimer’s disease biomarker

Autori: Kostas G. Mavrakis, Gerasimos Divaris, Maria Tampakaki, Saba N. Khan, Kishan Dholakia, Giannis Zacharakis
Pubblicato in: npj Nanophotonics, Numero 1, 2024, ISSN 2948-216X
Editore: nature
DOI: 10.1038/s44310-024-00036-3

Roadmap on chaos-inspired imaging technologies (CI2-Tech)

Autori: Joseph Rosen, Hilton B. de Aguiar, Vijayakumar Anand, YoonSeok Baek, Sylvain Gigan, Ryoichi Horisaki, Hervé Hugonnet, Saulius Juodkazis, KyeoReh Lee, Haowen Liang, Yikun Liu, Stephan Ludwig, Wolfgang Osten, YongKeun Park, Giancarlo Pedrini, Tushar Sarkar, Johannes Schindler, Alok Kumar Singh, Rakesh Kumar Singh, Guohai Situ, Mitsuo Takeda, Xiangsheng Xie, Wanqin Yang, Jianying Zhou
Pubblicato in: Applied Physics B, Numero 128, 2022, ISSN 0946-2171
Editore: Springer Verlag
DOI: 10.1007/s00340-021-07729-z

A Cost-Efficient Multiwavelength LED-Based System for Quantitative Photoacoustic Measurements

Autori: Michalis Orfanakis, George J. Tserevelakis, Giannis Zacharakis
Pubblicato in: Sensors, Numero 21/14, 2021, Pagina/e 4888, ISSN 1424-8220
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/s21144888

A proton-inhibited DEG/ENaC ion channel maintains neuronal ionstasis and promotes neuronal survival under stress

Autori: Dionysia Petratou, Martha Gjikolaj, Eva Kaulich, William Schafer, Nektarios Tavernarakis
Pubblicato in: iScience, 2023, ISSN 2589-0042
Editore: Dionysia Petratou
DOI: 10.1016/j.isci.2023.107117

To focus-match or not to focus-match inverse spatially offset Raman spectroscopy: a question of light penetration

Autori: Georgina E. Shillito, Lewis Mcmillan, Graham D. Bruce, and Kishan Dholakia
Pubblicato in: Optics Express, 2022, ISSN 1094-4087
Editore: Optical Society of America
DOI: 10.1364/oe.451496

Hybrid Raman and Partial Wave Spectroscopy Microscope for the Characterization of Molecular and Structural Alterations in Tissue

Autori: Elena Kriukova, Mikhail Mazurenka, Sabrina Marcazzan, Sarah Glasl, Michael Quante, Dieter Saur, Markus Tschurtschenthaler, Gerwin J. Puppels, Dimitris Gorpas, Vasilis Ntziachristos
Pubblicato in: Journal of Biophotonics, Numero 17, 2024, ISSN 1864-063X
Editore: Wiley - VCH Verlag GmbH & CO. KGaA
DOI: 10.1002/jbio.202400330

MitoSNARE Assembly and Disassembly Factors Regulate Basal Autophagy and Aging in C. elegans

Autori: Ilias Gkikas, Ioanna Daskalaki, Konstantinos Kounakis, Nektarios Tavernarakis, Eirini Lionaki
Pubblicato in: International Journal of Molecular Sciences, Numero 24, 2025, Pagina/e 4230, ISSN 1422-0067
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms24044230

Physics-based neural network for non-invasive control of coherent light in scattering media

Autori: Alexandra d’Arco, Fei Xia, Antoine Boniface, Jonathan Dong, Sylvain Gigan
Pubblicato in: Optics Express, Numero 30, 2024, Pagina/e 30845, ISSN 1094-4087
Editore: Optical Society of America
DOI: 10.1364/oe.465702

High precision photoacoustic interferometer for the determination of the speed of sound in liquid media

Autori: George J. Tserevelakis and Giannis Zacharakis
Pubblicato in: Optics Express, 2022
Editore: George J. Tserevelakis
DOI: 10.1364/oe.455557

Agar Gel as a Non-Invasive Coupling Medium for Reflectance Photoacoustic (PA) Imaging: Experimental Results on Wall-Painting Mock-Ups

Autori: Antonina Chaban , George J. Tserevelakis, Evgenia Klironomou, Giannis Zacharakis and Jana Striov
Pubblicato in: Journal of Imaging, 2022, ISSN 2313-433X
Editore: Antonina Chaban
DOI: 10.3390/jimaging8090235

Full image reconstruction in frequency-domain photoacoustic microscopy by means of a low-cost I/Q demodulator

Autori: George J. Tserevelakis, Kostas G. Mavrakis, Nikitas Kakakios, Giannis Zacharakis
Pubblicato in: Optics Letters, Numero 46/19, 2021, Pagina/e 4718, ISSN 0146-9592
Editore: Optical Society of America
DOI: 10.1364/ol.435146

Scattering correcting wavefront shaping for three-photon microscopy

Autori: Bernhard Rauer, Hilton B. de Aguiar, Laurent Bourdieu, Sylvain Gigan
Pubblicato in: Optics Letters, Numero 47, 2024, Pagina/e 6233, ISSN 0146-9592
Editore: Optical Society of America
DOI: 10.1364/ol.468834

Label-free super-resolution stimulated Raman scattering imaging of biomedical specimens

Autori: Julien Guilbert, Awoke Negash, Simon Labouesse, Sylvain Gigan, Anne Sentenac, Hilton B. de Aguiar
Pubblicato in: Advanced Imaging, Numero 1, 2024, Pagina/e 011004, ISSN 2959-9539
Editore: Researchng
DOI: 10.3788/ai.2024.10004

PML Differentially Regulates Growth and Invasion in Brain Cancer.

Autori: Maria Tampakaki; Maria Tampakaki; Mariam-Eleni Oraiopoulou; Eleftheria Tzamali; Giorgos Tzedakis; Takis Makatounakis; Giannis Zacharakis; Joseph Papamatheakis; Joseph Papamatheakis; Vangelis Sakkalis
Pubblicato in: Numero 12, Numero 11, 2021, ISSN 1422-0067
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms22126289

Neuronal atg1 Coordinates Autophagy Induction and Physiological Adaptations to Balance mTORC1 Signalling

Autori: Athanasios Metaxakis Michail Pavlidis and Nektarios Tavernarakis
Pubblicato in: Cells, 2023, ISSN 2073-4409
Editore: Athanasios Metaxakis
DOI: 10.3390/cells12162024

Non-Invasive Optoacoustic Imaging for In-Depth Cultural Heritage Diagnostics

Autori: George J. Tserevelakis, Eleanna Pirgianaki, Kristalia Melessanaki, Giannis Zacharakis, Costas Fotakis
Pubblicato in: Photonics, Numero 11, 2024, Pagina/e 902, ISSN 2304-6732
Editore: Photonics
DOI: 10.3390/photonics11100902

Spermidine is essential for fasting-mediated autophagy and longevity

Autori: Sebastian J. Hofer, Ioanna Daskalaki, Martina Bergmann, Jasna Friščić, Andreas Zimmermann, Melanie I. Mueller, Mahmoud Abdellatif, Raffaele Nicastro, Sarah Masser, Sylvère Durand, Alexander Nartey, Mara Waltenstorfer, Sarah Enzenhofer, Isabella Faimann, Verena Gschiel, Thomas Bajaj, Christine Niemeyer, Ilias Gkikas, Lukas Pein, Giulia Cerrato, Hui Pan, YongTian Liang, Jelena Tadic, Andrea Jerk
Pubblicato in: Nature Cell Biology, Numero 26, 2024, Pagina/e 1571-1584, ISSN 1465-7392
Editore: Nature Publishing Group
DOI: 10.1038/s41556-024-01468-x

Single-pixel transmission matrix recovery via two-photon fluorescence

Autori: Shupeng Zhao, Bernhard Rauer, Lorenzo Valzania, Jonathan Dong, Ruifeng Liu, Fuli Li, Sylvain Gigan, Hilton B. de Aguiar
Pubblicato in: Science Advances, Numero 10, 2024, ISSN 2375-2548
Editore: Science
DOI: 10.1126/sciadv.adi3442

Generation of Bessel-like beams with reduced sidelobes for enhanced light-sheet microscopy

Autori: Jerin Geogy George, Kishan Dholakia, and Shanti Bhattacharya
Pubblicato in: Optics Continuum, 2023, ISSN 2770-0208
Editore: Optica Publishing Group
DOI: 10.1364/optcon.493003

Non-invasive chemically selective energy delivery and focusing inside a scattering medium guided by Raman scattering

Autori: Bingxin Tian, Bernhard Rauer, Antoine Boniface, Jun Han, Sylvain Gigan, Hilton B. de Aguiar
Pubblicato in: Optics Letters, Numero 47, 2024, Pagina/e 2145, ISSN 0146-9592
Editore: Optical Society of America
DOI: 10.1364/ol.456411

Local coordination of mRNA storage and degradation near mitochondria modulates C. elegans ageing

Autori: Ioanna Daskalaki, Maria Markaki, Ilias Gkikas, Nektarios Tavernarakis
Pubblicato in: The EMBO Journal, 2023, ISSN 1460-2075
Editore: Ioanna Daskalaki
DOI: 10.15252/embj.2022112446

Revealing Underdrawings in Wall Paintings of Complex Stratigraphy with a Novel Reflectance Photoacoustic Imaging Prototype

Autori: Antonina Chaban, George J. Tserevelakis, Evgenia Klironomou, Raffaella Fontana, Giannis Zacharakis, Jana Striova
Pubblicato in: Journal of Imaging, Numero 7, 2025, Pagina/e 250, ISSN 2313-433X
Editore: MDPI
DOI: 10.3390/jimaging7120250

Phase conjugation with spatially incoherent light in complex media

Autori: YoonSeok Baek, Hilton B. de Aguiar & Sylvain Gigan
Pubblicato in: Nature Photonics, 2023, ISSN 1749-4885
Editore: Nature Pub. Group
DOI: 10.1038/s41566-023-01254-5

Imaging Parhyale hawaiensis embryogenesis with frequency domain photoacoustic microscopy: A novel tool in developmental biology

Autori: George J. Tserevelakis, Sofia Velentza, Ioannis Liaskas, Themis Archontidis, Anastasios Pavlopoulos, Giannis Zacharakis
Pubblicato in: Journal of Biophtonics, 2022, ISSN 1864-0648
Editore: Wiley-VCH GmbH
DOI: 10.1002/jbio.202200202

Experimentally unsupervised deconvolution for light-sheet microscopy with propagation-invariant beams

Autori: Philip Wijesinghe, Stella Corsetti, Darren J. X. Chow, Shuzo Sakata, Kylie R. Dunning and Kishan Dholakia
Pubblicato in: Light: Science & Applications, 2022, ISSN 2047-7538
Editore: USTAN
DOI: 10.1038/s41377-022-00975-6

Meshless Monte Carlo radiation transfer method for curved geometries using signed distance functions

Autori: Lewis McMillan, Graham D Bruce, Kishan Dholakia
Pubblicato in: Journal of Biomedical Optics, 2022, ISSN 1560-2281
Editore: SPIE
DOI: 10.1117/1.jbo.27.8.083003

Roadmap on computational methods in optical imaging and holography [invited]

Autori: Joseph Rosen, Simon Alford, Blake Allan, Vijayakumar Anand, Shlomi Arnon, Francis Gracy Arockiaraj, Jonathan Art, Bijie Bai, Ganesh M. Balasubramaniam, Tobias Birnbaum, Nandan S. Bisht, David Blinder, Liangcai Cao, Qian Chen, Ziyang Chen, Vishesh Dubey, Karen Egiazarian, Mert Ercan, Andrew Forbes, G. Gopakumar, Yunhui Gao, Sylvain Gigan, Paweł Gocłowski, Shivasubramanian Gopinath, Alon Greenbaum
Pubblicato in: Applied Physics B, Numero 130, 2024, ISSN 0946-2171
Editore: Springer Verlag
DOI: 10.1007/s00340-024-08280-3

UVA Hyperspectral Light-Sheet Microscopy for Volumetric Metabolic Imaging: Application to Preimplantation Embryo Development

Autori: Josephine Morizet, Darren Chow, Philip Wijesinghe, Erik Schartner, George Dwapanyin, Nicolas Dubost, Graham D. Bruce, Ellen Anckaert, Kylie Dunning, Kishan Dholakia
Pubblicato in: ACS Photonics, Numero 10, 2023, Pagina/e 4177-4187, ISSN 2330-4022
Editore: American Chemical Society
DOI: 10.1021/acsphotonics.3c00900

Assessing locomotory rate in response to food for the identification of neuronal and muscular defects in C. elegans

Autori: Dionysia Petratou, Persefoni Fragkiadaki, Eirini Lionaki, Nektarios Tavernarakis
Pubblicato in: STAR Protocols, Numero 5, 2024, Pagina/e 102801, ISSN 2666-1667
Editore: ScienceDirect
DOI: 10.1016/j.xpro.2023.102801

Optoacoustic interferometric characterization system (OPTICS) for the evaluation of fuel quality through speed of sound measurements

Autori: George J. Tserevelakis, Charalampos Astrinakis, Giannis Zacharakis
Pubblicato in: Ultrasonics, Numero 139, 2024, Pagina/e 107291, ISSN 0041-624X
Editore: Elsevier BV
DOI: 10.1016/j.ultras.2024.107291

Age-associated anatomical and physiological alterations in Caenorhabditis elegans

Autori: Emmanuel Spanoudakis, Nektarios Tavernarakis
Pubblicato in: Mechanisms of Ageing and Development, 2023, ISSN 1872-6216
Editore: Emmanuel Spanoudakis
DOI: 10.1016/j.mad.2023.111827

Remote control of autophagy and metabolism in the liver

Autori: Ilias Gkikas, Ioanna Daskalaki, Konstantinos Kounakis, Nektarios Tavernarakis and Eirini Lionaki
Pubblicato in: Int. J. Mol. Sci., 2023, ISSN 1422-0067
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms24044230

Bimodal optical and optoacoustic multiview microscope in the frequency-domain

Autori: Mikis Mylonakis, George J. Tserevelakis, Georgios Vlachos, Eleftheria Fanouraki, Anastasios Pavlopoulos, Michalis Pavlidis, Giannis Zacharakis
Pubblicato in: Optics Letters, Numero 49, 2024, Pagina/e 462, ISSN 0146-9592
Editore: Optical Society of America
DOI: 10.1364/ol.510384

Hybrid Fluorescence and Frequency-Domain Photoacoustic Microscopy for Imaging Development of Parhyale hawaiensis Embryos

Autori: George J. Tserevelakis, Emmanouela Tekonaki, Maria Kalogeridi, Ioannis Liaskas, Anastasios Pavlopoulos and Giannis Zacharakis
Pubblicato in: Photonics, 2023, ISSN 2304-6732
Editore: George J. Tserevelakis,
DOI: 10.3390/photonics10030264

Revealing Hidden Features in Multilayered Artworks by Means of an Epi-Illumination Photoacoustic Imaging System

Autori: George J. Tserevelakis, Antonina Chaban, Evgenia Klironomou, Kristalia Melessanaki, Jana Striova, Giannis Zacharakis
Pubblicato in: Journal of Imaging, Numero 7/9, 2021, Pagina/e 183, ISSN 2313-433X
Editore: George J. Tserevelakis, Antonina Chaban, Evgenia Klironomou, Kristalia Melessanaki, Jana Striova and Giannis Zacharakis
DOI: 10.3390/jimaging7090183

Hybrid Autofluorescence and Optoacoustic Microscopy for the Label-Free, Early and Rapid Detection of Pathogenic Infections in Vegetative Tissues

Autori: George J. Tserevelakis, Andreas Theocharis, Stavroula Spyropoulou, Emmanouil Trantas, , Dimitrios Goumas, Filippos Ververidis and Giannis Zacharakis
Pubblicato in: Journal of Imaging, 2023, ISSN 2313-433X
Editore: George J. Tserevelakis
DOI: 10.3390/jimaging9090176

Towards affordable biomedical imaging: Recent advances in low‐cost, high‐resolution optoacoustic microscopy

Autori: Pavlos Kalitsounakis, Giannis Zacharakis, George J. Tserevelakis
Pubblicato in: Journal of Microscopy, 2024, ISSN 0022-2720
Editore: Blackwell Publishing Inc.
DOI: 10.1111/jmi.13378

Neurophotonics beyond the surface: unmasking the brain’s complexity exploiting optical scattering

Autori: Fei Xia, Caio Vaz Rimoli, Walther Akemann, Cathie Ventalon, Laurent Bourdieu, Sylvain Gigan, Hilton B. de Aguiar
Pubblicato in: Neurophotonics, Numero 11, 2024, ISSN 2329-423X
Editore: SPIE
DOI: 10.1117/1.nph.11.s1.s11510

Other (1)

Label-free super-resolution chemical imaging of biomedical specimens

Autori: Julien Guilbert, Awoke Negash, Simon Labouesse, Sylvain Gigan, Anne Sentenac, Hilton B. de Aguiar
Pubblicato in: bioRxiv, 2021
Editore: Cold Spring Harbor Laboratory
DOI: 10.1101/2021.05.14.444185

Diritti di proprietà intellettuale

Patent (1)

AN ASSEMBLY OF OPTICAL ELEMENTS AND A METHOD FOR CONTROLLING LIGHT

Numero candidatura/pubblicazione: 22 164624
Data: 2022-03-28
Candidato/i: IDRYMA TECHNOLOGIAS KAI EREVNAS