CAPTURING THE PHYSICS OF LIFE ON 3D-TRAFFICKING SUBCELLULAR NANOSYSTEMS

Información del proyecto

CAPTUR3D

Identificador del acuerdo de subvención: 866127

DOI

10.3030/866127

Proyecto cerrado

Fecha de la firma de la CE 16 Marzo 2020

Fecha de inicio 1 Marzo 2021

Fecha de finalización 31 Agosto 2026

Financiado con arreglo a

• EXCELLENT SCIENCE - European Research Council (ERC)

Coste total

€ 1 985 750,00

Aportación de la UE

€ 1 985 750,00

Coordinado por

SCUOLA NORMALE SUPERIORE
Italy

CORDIS proporciona enlaces a los documentos públicos y las publicaciones de los proyectos de los programas marco HORIZONTE.

Los enlaces a los documentos y las publicaciones de los proyectos del Séptimo Programa Marco, así como los enlaces a algunos tipos de resultados específicos, como conjuntos de datos y «software», se obtienen dinámicamente de OpenAIRE .

Publicaciones

Peer reviewed articles (18)

Optical Nanoscopy of Cytokine-Induced Structural Alteration of Endoplasmic Reticulum and Golgi Apparatus in Insulin Secreting Cells

Autores: Licia Anna Pugliese, Valentina De Lorenzi, Marta Tesi, Piero Marchetti, Francesco Cardarelli
Publicado en: International Journal of Molecular Sciences, 2024, ISSN 1422-0067
Editor: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.20944/preprints202408.1000.v1

Mechanistic Insights into the Superior DNA Delivery Efficiency of Multicomponent Lipid Nanoparticles: An In Vitro and In Vivo Study

Autores: Erica Quagliarini; Junbiao Wang; Serena Renzi; Lishan Cui; Luca Digiacomo; Gianmarco Ferri; Luca Pesce; Valentina De Lorenzi; Giulia Matteoli; Heinz Amenitsch; Laura Masuelli; Roberto Bei; Daniela Pozzi; Augusto Amici; Francesco Cardarelli; Cristina Marchini; Giulio Caracciolo
Publicado en: ACS Applied Materials and Interfaces, 2022, ISSN 1944-8244
Editor: American Chemical Society
DOI: 10.1021/acsami.2c20019

Measuring pH in insulin secretory granules by phasor-based fluorescence lifetime imaging of a genetically encoded sensor

Autores: Valentina De Lorenzi, Samuele Ghignoli, Mario Bernardi, Giulia Matteoli, Gianmarco Ferri, Barbara Storti, Ranieri Bizzarri, Francesco Cardarelli
Publicado en: Communications Biology, Edición 8, 2025, ISSN 2399-3642
Editor: Springer Science and Business Media LLC
DOI: 10.1038/s42003-025-07758-w

Toward an integrated view of nuclear pore transport

Autores: Luca Lanzano, Francesco Cardarelli
Publicado en: Trends in Cell Biology, Edición 36, 2026, Página(s) 528-531, ISSN 0962-8924
Editor: Elsevier BV
DOI: 10.1016/j.tcb.2026.04.002

β-Cell Pathophysiology: A Review of Advanced Optical Microscopy Applications

Autores: Gianmarco Ferri; Luca Pesce; Marta Tesi; Piero Marchetti; Francesco Cardarelli
Publicado en: International Journal of Molecular Sciences, 2021, ISSN 1422-0067
Editor: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms222312820

Functional recovery of islet β cells in human type 2 diabetes: Transcriptome signatures unveil therapeutic approaches

Autores: Mara Suleiman, Toshiaki Sawatani, Marta Tesi, Xiaoyan Yi, Theodora Papadopoulou, Chantal Rufer, Maria Lytrivi, Emanuele Bosi, Frederic Burdet, Federica Fantuzzi, Carmela De Luca, Guido Sebastiani, Chiara Saponaro, Licia Anna Pugliese, Silvia Del Guerra, Alessandro Pocai, Paolo De Simone, Davide Ghinolfi, Ugo Boggi, Camille Kessler, Giuseppina Emanuela Grieco, Daniela Fignani, Julie Kerr-Conte, Fra
Publicado en: Science Advances, Edición 11, 2025, ISSN 2375-2548
Editor: American Association for the Advancement of Science (AAAS)
DOI: 10.1126/sciadv.ads2905

Pioglitazone Phases and Metabolic Effects in Nanoparticle-Treated Cells Analyzed via Rapid Visualization of FLIM Images

Autores: Biagio Todaro, Luca Pesce, Francesco Cardarelli, Stefano Luin
Publicado en: Molecules, Edición 29, 2025, Página(s) 2137, ISSN 1420-3049
Editor: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/molecules29092137

Unveiling nanoscale optical signatures of cytokine‑induced β‑cell dysfunction

Autores: Licia Anna Pugliese, Valentina De Lorenzi, Mario Bernardi, Samuele Ghignoli, Marta Tesi, Piero Marchetti, Luca Pesce, Francesco Cardarelli
Publicado en: Scientific Reports, 2023, ISSN 2045-2322
Editor: Nature Publishing Group
DOI: 10.1038/s41598-023-40272-9

Pair correlation microscopy of intracellular molecular transport

Autores: Julissa Sanchez-Velasquez, Ashleigh Solano, Michelle A. Digman, Enrico Gratton, Francesco Cardarelli, Elizabeth Hinde
Publicado en: Nature Protocols, Edición 20, 2025, Página(s) 1651-1677, ISSN 1754-2189
Editor: Nature Publishing Group
DOI: 10.1038/s41596-024-01097-6

Fluorescence Lifetime Nanoscopy of Liposomal Irinotecan Onivyde: From Manufacturing to Intracellular Processing

Autores: Mario Bernardi, Giovanni Signore, Aldo Moscardini, Licia Anna Pugliese, Luca Pesce, Fabio Beltram, Francesco Cardarelli
Publicado en: ACS Applied Bio Materials, 2023, ISSN 2576-6422
Editor: American Chemical Society
DOI: 10.1021/acsabm.3c00478

Single-cell imaging of α and β cell metabolic response to glucose in living human Langerhans islets

Autores: Fabio Azzarello, Luca Pesce, Valentina De Lorenzi, Gianmarco Ferri, Marta Tesi, Silvia Del Guerra, Piero Marchetti, Francesco Cardarelli
Publicado en: Communications Biology, 2022, ISSN 2399-3642
Editor: Nature Publishing Group
DOI: 10.1038/s42003-022-04215-w

Spatiotemporal Correlation Spectroscopy Reveals a Protective Effect of Peptide-Based GLP-1 Receptor Agonism against Lipotoxicity on Insulin Granule Dynamics in Primary Human β-Cells.

Autores: Gianmarco Ferri; Marta Tesi; Luca Pesce; Marco Bugliani; Francesca Grano; Margherita Occhipinti; Mara Suleiman; Carmela De Luca; Lorella Marselli; Piero Marchetti; Francesco Cardarelli
Publicado en: Pharmaceutics, 2021, ISSN 1999-4923
Editor: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/pharmaceutics13091403

Probing Structural and Dynamic Properties of Trafficking Subcellular Nanostructures by Spatiotemporal Fluctuation Spectroscopy

Autores: Gianmarco Ferri, Fabio Azzarello, Francesca D'Autilia, Francesco Cardarelli
Publicado en: Journal of Visualized Experiments, Edición 1940087X, 2021, ISSN 1940-087X
Editor: MYJoVE Corporation
DOI: 10.3791/62790

Spatiotemporal Analysis of Glucagon Secretory Granule Dynamics

Autores: Samuele Ghignoli, Valentina De Lorenzi, Gianmarco Ferri, Licia Anna Pugliese, Marta Tesi, Piero Marchetti, Stefano Luin, Francesco Cardarelli
Publicado en: Traffic, Edición 26, 2025, ISSN 1398-9219
Editor: Blackwell Publishing Inc.
DOI: 10.1111/tra.70019

Investigating the mechanism of action of DNA-loaded PEGylatedlipid nanoparticles

Autores: Luca Digiacomo, Serena Renzi, Erica Quagliarini, Daniela Pozzi, Heinz Amenitsch, Gianmarco Ferri, Luca Pesce, Valentina De Lorenzi, Giulia Matteoli, Francesco Cardarelli, Giulio Caracciolo
Publicado en: Nanomedicine: Nanotechnology, Biology, and Medicine, 2023, ISSN 1549-9634
Editor: Elsevier BV
DOI: 10.1016/j.nano.2023.102697

Measuring molecular diffusion in dynamic subcellular nanostructures by fast Raster Image Correlation Spectroscopy and 3D orbital tracking

Autores: Filippo Begarani; Francesca D’Autilia; Gianmarco Ferri; Luca Pesce; Fabio Azzarello; Valentina De Lorenzi; William Durso; Ambra Del Grosso; Marco Cecchini; Francesco Cardarelli
Publicado en: International Journal of Molecular Sciences, 2022, ISSN 1422-0067
Editor: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms23147623

Machine-learning-guided recognition of α and β cells from label-free infrared micrographs of living human islets of Langerhans

Autores: Fabio Azzarello, Francesco Carli, Valentina De Lorenzi, Marta Tesi, Piero Marchetti, Fabio Beltram, Francesco Raimondi, Francesco Cardarelli
Publicado en: Scientific Reports, Edición 14, 2024, ISSN 2045-2322
Editor: Nature Publishing Group
DOI: 10.1038/s41598-024-65161-7

Metabolic trajectories in developing human neocortical neurons

Autores: Gianmarco Ferri, Francesco Finamore, Francesco Greco, Alessio Zanelli, Fabrizia Carli, Alessandro Passera, Elisa Degl’Innocenti, Francesco Olimpico, Riccardo Ducoli, Agnese Sgalippa, Gian Carlo Demontis, Amalia Gastaldelli, Francesco Cardarelli, Marco Onorati, Liam A. McDonnell, Maria Teresa Dell’Anno
Publicado en: Proceedings of the National Academy of Sciences, Edición 123, 2026, ISSN 0027-8424
Editor: National Academy of Sciences
DOI: 10.1073/pnas.2509980123

Other (3)

Measuring pH in insulin secretory granules using phasor fluorescence lifetime imaging of a genetically encoded sensor

Autores: Francesco Cardarelli, Valentina De Lorenzi, Samuele Ghignoli, Mario Bernardi, Giulia Matteoli, Barbara Storti, Ranieri Bizzarri
Publicado en: Research Square, 2025
Editor: Springer Science and Business Media LLC
DOI: 10.21203/rs.3.rs-4968583/v1

Phasor Identifier: A Cloud-based Analysis of Phasor-FLIM Data on Python Notebooks

Autores: Mario Bernardi, Francesco Cardarelli
Publicado en: Biorxiv, 2023
Editor: Cold Spring Harbor
DOI: 10.1101/2023.08.30.555392

Direct optical nanoscopy unveils signatures of cytokine-induced β-cell structural and functional stress

Autores: Licia Anna Pugliese, Valentina De Lorenzi, Mario Bernardi, Samuele Ghignoli, Marta Tesi, Piero Marchetti, Francesco Cardarelli, Luca Pesce
Publicado en: BiorXiv, 2023
Editor: Cold Spring Harbor
DOI: 10.1101/2023.02.20.529190

Non-peer reviewed articles (1)

BPS2025 - Nanoscale investigation of cytokine-induced stress in insulin-secreting cells and tissues via expansion microscopy

Autores: Licia Anna Pugliese, Valentina De Lorenzi, Samuele Ghignoli, Mario Bernardi, Luca Pesce, Marta Tesi, Mara Suleiman, Carmela De Luca, Lorella Marselli, Piero Marchetti, Ha V. Vo, Allison Lindquist, Yongxin Zhao, Francesco Cardarelli
Publicado en: Biophysical Journal, Edición 124, 2026, Página(s) 590a-591a, ISSN 0006-3495
Editor: Biophysical Society
DOI: 10.1016/j.bpj.2024.11.3060

Conference proceedings (4)

Single-cell-resolution imaging of alpha/beta cell metabolic response to glucose stimulation in living human-derived Langerhans islets

Autores: Francesco Cardarelli; Fabio Azzarello; Luca Pesce; Valentina De Lorenzi; Gianmarco Ferri; Marta Tesi; Silvia Del Guerra; Piero Marchetti
Publicado en: Edición 3, 2022
Editor: Springer

Single-cell-resolution imaging of alpha/beta cell metabolic response to glucose stimulation in living human-derived Langerhans islets

Autores: Francesco Cardarelli, Fabio Azzarello, Luca Pesce, Valentina De Lorenzi, Gianmarco Ferri, Marta Tesi, Silvia Del Guerra, Piero Marchetti
Publicado en: DIABETOLOGIA - ISSN: 1432-0428, 2022, Página(s) S18-S19
Editor: SPRINGER
DOI: 10.1007/s00125-022-05755-w

15th EBSA congress, June 30 – July 4, 2025, Rome, Italy – Abstracts

Autores: Samuele Ghignoli; Valentina De Lorenzi; Gianmarco Ferri; Ranieri Bizzarri; Francesco Cardarelli
Publicado en: European Biophysics Journal, Edición 54, 2025, Página(s) 1-273, ISSN 0175-7571
Editor: Springer Verlag
DOI: 10.1007/s00249-025-01757-9

Direct optical nanoscopy unveils signatures of cytokine-induced β-cell structural and functional stress

Autores: Licia Anna Pugliese, Valentina De Lorenzi, Mario Bernardi, Samuele Ghignoli, Marta Tesi, Piero Marchetti, Luca Pesce, Francesco Cardarelli
Publicado en: DIABETOLOGIA - ISSN: 1432-0428, 2023
Editor: Springer
DOI: 10.1007/s00125-023-05969-6