CAPTURING THE PHYSICS OF LIFE ON 3D-TRAFFICKING SUBCELLULAR NANOSYSTEMS

Informacje na temat projektu

CAPTUR3D

Identyfikator umowy o grant: 866127

DOI

10.3030/866127

Projekt został zamknięty

Data podpisania przez KE 16 Marca 2020

Data rozpoczęcia 1 Marca 2021

Data zakończenia 31 Sierpnia 2026

Finansowanie w ramach

• EXCELLENT SCIENCE - European Research Council (ERC)

Koszt całkowity

€ 1 985 750,00

Wkład UE

€ 1 985 750,00

Koordynowany przez

SCUOLA NORMALE SUPERIORE
Italy

CORDIS oferuje możliwość skorzystania z odnośników do publicznie dostępnych publikacji i rezultatów projektów realizowanych w ramach programów ramowych HORYZONT.

Odnośniki do rezultatów i publikacji związanych z poszczególnymi projektami 7PR, a także odnośniki do niektórych konkretnych kategorii wyników, takich jak zbiory danych i oprogramowanie, są dynamicznie pobierane z systemu OpenAIRE .

Publikacje

Peer reviewed articles (18)

Optical Nanoscopy of Cytokine-Induced Structural Alteration of Endoplasmic Reticulum and Golgi Apparatus in Insulin Secreting Cells

Autorzy: Licia Anna Pugliese, Valentina De Lorenzi, Marta Tesi, Piero Marchetti, Francesco Cardarelli
Opublikowane w: International Journal of Molecular Sciences, 2024, ISSN 1422-0067
Wydawca: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.20944/preprints202408.1000.v1

Mechanistic Insights into the Superior DNA Delivery Efficiency of Multicomponent Lipid Nanoparticles: An In Vitro and In Vivo Study

Autorzy: Erica Quagliarini; Junbiao Wang; Serena Renzi; Lishan Cui; Luca Digiacomo; Gianmarco Ferri; Luca Pesce; Valentina De Lorenzi; Giulia Matteoli; Heinz Amenitsch; Laura Masuelli; Roberto Bei; Daniela Pozzi; Augusto Amici; Francesco Cardarelli; Cristina Marchini; Giulio Caracciolo
Opublikowane w: ACS Applied Materials and Interfaces, 2022, ISSN 1944-8244
Wydawca: American Chemical Society
DOI: 10.1021/acsami.2c20019

Measuring pH in insulin secretory granules by phasor-based fluorescence lifetime imaging of a genetically encoded sensor

Autorzy: Valentina De Lorenzi, Samuele Ghignoli, Mario Bernardi, Giulia Matteoli, Gianmarco Ferri, Barbara Storti, Ranieri Bizzarri, Francesco Cardarelli
Opublikowane w: Communications Biology, Numer 8, 2025, ISSN 2399-3642
Wydawca: Springer Science and Business Media LLC
DOI: 10.1038/s42003-025-07758-w

Toward an integrated view of nuclear pore transport

Autorzy: Luca Lanzano, Francesco Cardarelli
Opublikowane w: Trends in Cell Biology, Numer 36, 2026, Strona(/y) 528-531, ISSN 0962-8924
Wydawca: Elsevier BV
DOI: 10.1016/j.tcb.2026.04.002

β-Cell Pathophysiology: A Review of Advanced Optical Microscopy Applications

Autorzy: Gianmarco Ferri; Luca Pesce; Marta Tesi; Piero Marchetti; Francesco Cardarelli
Opublikowane w: International Journal of Molecular Sciences, 2021, ISSN 1422-0067
Wydawca: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms222312820

Functional recovery of islet β cells in human type 2 diabetes: Transcriptome signatures unveil therapeutic approaches

Autorzy: Mara Suleiman, Toshiaki Sawatani, Marta Tesi, Xiaoyan Yi, Theodora Papadopoulou, Chantal Rufer, Maria Lytrivi, Emanuele Bosi, Frederic Burdet, Federica Fantuzzi, Carmela De Luca, Guido Sebastiani, Chiara Saponaro, Licia Anna Pugliese, Silvia Del Guerra, Alessandro Pocai, Paolo De Simone, Davide Ghinolfi, Ugo Boggi, Camille Kessler, Giuseppina Emanuela Grieco, Daniela Fignani, Julie Kerr-Conte, Fra
Opublikowane w: Science Advances, Numer 11, 2025, ISSN 2375-2548
Wydawca: American Association for the Advancement of Science (AAAS)
DOI: 10.1126/sciadv.ads2905

Pioglitazone Phases and Metabolic Effects in Nanoparticle-Treated Cells Analyzed via Rapid Visualization of FLIM Images

Autorzy: Biagio Todaro, Luca Pesce, Francesco Cardarelli, Stefano Luin
Opublikowane w: Molecules, Numer 29, 2025, Strona(/y) 2137, ISSN 1420-3049
Wydawca: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/molecules29092137

Unveiling nanoscale optical signatures of cytokine‑induced β‑cell dysfunction

Autorzy: Licia Anna Pugliese, Valentina De Lorenzi, Mario Bernardi, Samuele Ghignoli, Marta Tesi, Piero Marchetti, Luca Pesce, Francesco Cardarelli
Opublikowane w: Scientific Reports, 2023, ISSN 2045-2322
Wydawca: Nature Publishing Group
DOI: 10.1038/s41598-023-40272-9

Pair correlation microscopy of intracellular molecular transport

Autorzy: Julissa Sanchez-Velasquez, Ashleigh Solano, Michelle A. Digman, Enrico Gratton, Francesco Cardarelli, Elizabeth Hinde
Opublikowane w: Nature Protocols, Numer 20, 2025, Strona(/y) 1651-1677, ISSN 1754-2189
Wydawca: Nature Publishing Group
DOI: 10.1038/s41596-024-01097-6

Fluorescence Lifetime Nanoscopy of Liposomal Irinotecan Onivyde: From Manufacturing to Intracellular Processing

Autorzy: Mario Bernardi, Giovanni Signore, Aldo Moscardini, Licia Anna Pugliese, Luca Pesce, Fabio Beltram, Francesco Cardarelli
Opublikowane w: ACS Applied Bio Materials, 2023, ISSN 2576-6422
Wydawca: American Chemical Society
DOI: 10.1021/acsabm.3c00478

Single-cell imaging of α and β cell metabolic response to glucose in living human Langerhans islets

Autorzy: Fabio Azzarello, Luca Pesce, Valentina De Lorenzi, Gianmarco Ferri, Marta Tesi, Silvia Del Guerra, Piero Marchetti, Francesco Cardarelli
Opublikowane w: Communications Biology, 2022, ISSN 2399-3642
Wydawca: Nature Publishing Group
DOI: 10.1038/s42003-022-04215-w

Spatiotemporal Correlation Spectroscopy Reveals a Protective Effect of Peptide-Based GLP-1 Receptor Agonism against Lipotoxicity on Insulin Granule Dynamics in Primary Human β-Cells.

Autorzy: Gianmarco Ferri; Marta Tesi; Luca Pesce; Marco Bugliani; Francesca Grano; Margherita Occhipinti; Mara Suleiman; Carmela De Luca; Lorella Marselli; Piero Marchetti; Francesco Cardarelli
Opublikowane w: Pharmaceutics, 2021, ISSN 1999-4923
Wydawca: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/pharmaceutics13091403

Probing Structural and Dynamic Properties of Trafficking Subcellular Nanostructures by Spatiotemporal Fluctuation Spectroscopy

Autorzy: Gianmarco Ferri, Fabio Azzarello, Francesca D'Autilia, Francesco Cardarelli
Opublikowane w: Journal of Visualized Experiments, Numer 1940087X, 2021, ISSN 1940-087X
Wydawca: MYJoVE Corporation
DOI: 10.3791/62790

Spatiotemporal Analysis of Glucagon Secretory Granule Dynamics

Autorzy: Samuele Ghignoli, Valentina De Lorenzi, Gianmarco Ferri, Licia Anna Pugliese, Marta Tesi, Piero Marchetti, Stefano Luin, Francesco Cardarelli
Opublikowane w: Traffic, Numer 26, 2025, ISSN 1398-9219
Wydawca: Blackwell Publishing Inc.
DOI: 10.1111/tra.70019

Investigating the mechanism of action of DNA-loaded PEGylatedlipid nanoparticles

Autorzy: Luca Digiacomo, Serena Renzi, Erica Quagliarini, Daniela Pozzi, Heinz Amenitsch, Gianmarco Ferri, Luca Pesce, Valentina De Lorenzi, Giulia Matteoli, Francesco Cardarelli, Giulio Caracciolo
Opublikowane w: Nanomedicine: Nanotechnology, Biology, and Medicine, 2023, ISSN 1549-9634
Wydawca: Elsevier BV
DOI: 10.1016/j.nano.2023.102697

Measuring molecular diffusion in dynamic subcellular nanostructures by fast Raster Image Correlation Spectroscopy and 3D orbital tracking

Autorzy: Filippo Begarani; Francesca D’Autilia; Gianmarco Ferri; Luca Pesce; Fabio Azzarello; Valentina De Lorenzi; William Durso; Ambra Del Grosso; Marco Cecchini; Francesco Cardarelli
Opublikowane w: International Journal of Molecular Sciences, 2022, ISSN 1422-0067
Wydawca: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms23147623

Machine-learning-guided recognition of α and β cells from label-free infrared micrographs of living human islets of Langerhans

Autorzy: Fabio Azzarello, Francesco Carli, Valentina De Lorenzi, Marta Tesi, Piero Marchetti, Fabio Beltram, Francesco Raimondi, Francesco Cardarelli
Opublikowane w: Scientific Reports, Numer 14, 2024, ISSN 2045-2322
Wydawca: Nature Publishing Group
DOI: 10.1038/s41598-024-65161-7

Metabolic trajectories in developing human neocortical neurons

Autorzy: Gianmarco Ferri, Francesco Finamore, Francesco Greco, Alessio Zanelli, Fabrizia Carli, Alessandro Passera, Elisa Degl’Innocenti, Francesco Olimpico, Riccardo Ducoli, Agnese Sgalippa, Gian Carlo Demontis, Amalia Gastaldelli, Francesco Cardarelli, Marco Onorati, Liam A. McDonnell, Maria Teresa Dell’Anno
Opublikowane w: Proceedings of the National Academy of Sciences, Numer 123, 2026, ISSN 0027-8424
Wydawca: National Academy of Sciences
DOI: 10.1073/pnas.2509980123

Other (3)

Measuring pH in insulin secretory granules using phasor fluorescence lifetime imaging of a genetically encoded sensor

Autorzy: Francesco Cardarelli, Valentina De Lorenzi, Samuele Ghignoli, Mario Bernardi, Giulia Matteoli, Barbara Storti, Ranieri Bizzarri
Opublikowane w: Research Square, 2025
Wydawca: Springer Science and Business Media LLC
DOI: 10.21203/rs.3.rs-4968583/v1

Phasor Identifier: A Cloud-based Analysis of Phasor-FLIM Data on Python Notebooks

Autorzy: Mario Bernardi, Francesco Cardarelli
Opublikowane w: Biorxiv, 2023
Wydawca: Cold Spring Harbor
DOI: 10.1101/2023.08.30.555392

Direct optical nanoscopy unveils signatures of cytokine-induced β-cell structural and functional stress

Autorzy: Licia Anna Pugliese, Valentina De Lorenzi, Mario Bernardi, Samuele Ghignoli, Marta Tesi, Piero Marchetti, Francesco Cardarelli, Luca Pesce
Opublikowane w: BiorXiv, 2023
Wydawca: Cold Spring Harbor
DOI: 10.1101/2023.02.20.529190

Non-peer reviewed articles (1)

BPS2025 - Nanoscale investigation of cytokine-induced stress in insulin-secreting cells and tissues via expansion microscopy

Autorzy: Licia Anna Pugliese, Valentina De Lorenzi, Samuele Ghignoli, Mario Bernardi, Luca Pesce, Marta Tesi, Mara Suleiman, Carmela De Luca, Lorella Marselli, Piero Marchetti, Ha V. Vo, Allison Lindquist, Yongxin Zhao, Francesco Cardarelli
Opublikowane w: Biophysical Journal, Numer 124, 2026, Strona(/y) 590a-591a, ISSN 0006-3495
Wydawca: Biophysical Society
DOI: 10.1016/j.bpj.2024.11.3060

Conference proceedings (4)

Single-cell-resolution imaging of alpha/beta cell metabolic response to glucose stimulation in living human-derived Langerhans islets

Autorzy: Francesco Cardarelli; Fabio Azzarello; Luca Pesce; Valentina De Lorenzi; Gianmarco Ferri; Marta Tesi; Silvia Del Guerra; Piero Marchetti
Opublikowane w: Numer 3, 2022
Wydawca: Springer

Single-cell-resolution imaging of alpha/beta cell metabolic response to glucose stimulation in living human-derived Langerhans islets

Autorzy: Francesco Cardarelli, Fabio Azzarello, Luca Pesce, Valentina De Lorenzi, Gianmarco Ferri, Marta Tesi, Silvia Del Guerra, Piero Marchetti
Opublikowane w: DIABETOLOGIA - ISSN: 1432-0428, 2022, Strona(/y) S18-S19
Wydawca: SPRINGER
DOI: 10.1007/s00125-022-05755-w

15th EBSA congress, June 30 – July 4, 2025, Rome, Italy – Abstracts

Autorzy: Samuele Ghignoli; Valentina De Lorenzi; Gianmarco Ferri; Ranieri Bizzarri; Francesco Cardarelli
Opublikowane w: European Biophysics Journal, Numer 54, 2025, Strona(/y) 1-273, ISSN 0175-7571
Wydawca: Springer Verlag
DOI: 10.1007/s00249-025-01757-9

Direct optical nanoscopy unveils signatures of cytokine-induced β-cell structural and functional stress

Autorzy: Licia Anna Pugliese, Valentina De Lorenzi, Mario Bernardi, Samuele Ghignoli, Marta Tesi, Piero Marchetti, Luca Pesce, Francesco Cardarelli
Opublikowane w: DIABETOLOGIA - ISSN: 1432-0428, 2023
Wydawca: Springer
DOI: 10.1007/s00125-023-05969-6