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Mechanisms of Apomictic Developments

CORDIS provides links to public deliverables and publications of HORIZON projects.

Links to deliverables and publications from FP7 projects, as well as links to some specific result types such as dataset and software, are dynamically retrieved from OpenAIRE .

Deliverables

List of candidate genes validated across several apomictic species by using the transcriptomic data available from previous projects (opens in new window)
List of Arabidopsis mutants affected in AUX and/or CK pathways and defective in MMC fate (opens in new window)
Characterisation of MMC fate, organ-level and cellular growth phenotypes in Arabidopsis transgenic lines perturbing cell wall composition in specific domains of the ovule (opens in new window)

Report on the characterisation of MMC fate, organ-level and cellular growth phenotypes in Arabidopsis transgenic lines perturbing cell wall composition in specific domains of the ovule

Quantification of cell proliferation in the selected variants (P. rufum, maize, Arabidopsis) and their respective controls (opens in new window)

Report on the quantification of cell proliferation in the selected variants (P. rufum, maize, Arabidopsis) and their respective controls

List of rice/maize/Paspalum mutants obtained through CRISPR/Cas (opens in new window)

List of rice/maize/Paspalum mutants obtained through CRISPR/Cas for genes selected

Quantification of anisotropy of cell growth, cell wall changes in the selected variants (P. rufum, maize, Arabidopsis) and their respective controls (opens in new window)

Report on the quantification of anisotropy of cell growth, cell wall changes in the selected variants (P. rufum, maize, Arabidopsis) and their respective controls

List of genes producing alternative splice variants in apomictic plants compared to sexual plants (opens in new window)
List of mutants/genetically modified lines with repressed or enhanced activity of genes involved in the sexuality-apomixis switch (opens in new window)
Characterization of reproductive phenotypes observed in Paspalum spp. and E. curvula transgenic lines carrying upregulated/downregulated candidate genes for apomixis (opens in new window)

Reprot on the characterization of reproductive phenotypes observed in Paspalum spp. and E. curvula transgenic lines carrying upregulated/downregulated candidate genes for apomixis

3D cellular atlas of early ovules in the selected variants (P. rufum, maize, Arabidopsis), and their respective controls (opens in new window)
List of genes showing alterations in post-transcriptional splicing and functional analysis of selected candidates (opens in new window)
Epigenome landscapes in floral tissues of apomictic and sexual Paspalum and Eragrostis, including candidates under epigenetic control (opens in new window)

Report on epigenome landscapes in floral tissues of apomictic and sexual Paspalum and Eragrostis, including candidates under epigenetic control

CRISP/cas9 genome editing in maize/rice (opens in new window)

Reprot on CRISP/cas9 genome editing in maize/rice

ACL homoelogous regions in sexual grass genomes and list of candidate genes (opens in new window)

Report for ACL homoelogous regions in sexual grass genomes and list of candidate genes

Delineate the structure and evolution of the ACL of Paspalum spp. and E. curvula and propose functional roles of genes located in it (opens in new window)

Report on both structure and evolution of the ACL of Paspalum spp. and E. curvula and hypothesis for the functional roles of genes located in it

Ploidy analysis of the obtained mutants with apomictic phenotypes (opens in new window)

Report on ploidy analysis of the obtained mutants with apomictic phenotypes

Biosensors analysis for AUX and CK signaling during the first stages of ovule development in P. notatum (opens in new window)

Report for biosensors analysis for AUX and CK signaling during the first stages of ovule development in P. notatum

mRNA ISH expression patterns of known MMC genes to identify female germline markers in P. rufum and maize (opens in new window)

Report on mRNA ISH expression patterns of known MMC genes to identify female germline markers in P. rufum and maize

Quantification of AUX and CK levels in sexual and apomictic ovules (opens in new window)

Report on the quantification of AUX and CK levels in sexual and apomictic ovules

List of regulatory regions in megasporogenesis, megagametogenesis and embryo patterning genes showing deposition of epigenetic marks involved in their transcriptional or post-transcriptional regulation (opens in new window)
Website (opens in new window)

Web site scientific section

Video of MAD achievements (opens in new window)

Short presentation video of MAD Consortium main achievements

Publications

A procedure for Dex-induced gene transactivation in Arabidopsis ovules (opens in new window)

Author(s): Schubert, Jasmin; Li, Yanru; Mendes, Marta A; Fei, Danli; Dickinson, Hugh; Moore, Ian; Baroux, Célia
Published in: Plant Methods, Issue 18, 2022, ISSN 1746-4811
Publisher: BioMed Central
DOI: 10.1186/s13007-022-00879-x

Auxin response factor 10 insensitive to miR160regulation induces apospory-like phenotypes in Arabidopsis (opens in new window)

Author(s): Silvina Pessino, Mara Cucinotta, Carolina Colono, Elena Costantini, Davide Perrone, Maurizio Di Marzo, Giada Callizaya Terceros, Rosanna Petrella, Chiara Mizzotti, Celeste Azzaro, Maricel Podio, Gianpiero Marconi, Emidio Albertini, Hugh Dickinson, Lucia Colombo, Marta A. Mendes
Published in: iScience, Issue 27, 2025, Page(s) 111115, ISSN 2589-0042
Publisher: Elsevier BV
DOI: 10.1016/j.isci.2024.111115

Differentially methylated genes involved in reproduction and ploidy levels in recent diploidized and tetraploidized Eragrostis curvula genotypes (opens in new window)

Author(s): J. Carballo, A. Achilli, F. Hernández, M. Bocchini, M. C. Pasten, G. Marconi, E. Albertini, D. Zappacosta, V. Echenique
Published in: Plant Reproduction, Issue 37, 2024, Page(s) 133-145, ISSN 2194-7953
Publisher: Springer Verlag
DOI: 10.1007/s00497-023-00490-7

Transcriptome-guided breeding for Paspalum notatum: producing apomictic hybrids with enhanced omega-3 content (opens in new window)

Author(s): Lara Marino, Silvia Altabe, Carolina Marta Colono, Maricel Podio, Juan Pablo Amelio Ortiz, David Balaban, Juliana Stein, Nicolás Spoto, Carlos Acuña, Lorena Adelina Siena, José Gerde, Emidio Albertini, Silvina Claudia Pessino
Published in: Theoretical and Applied Genetics, Issue 138, 2025, ISSN 0040-5752
Publisher: Springer Verlag
DOI: 10.1007/s00122-024-04788-6

Chromosome-scale genome assembly and annotation of Paspalum notatum Flüggé var. saurae (opens in new window)

Author(s): Juan Manuel Vega, Maricel Podio, Julie Orjuela, Lorena A. Siena, Silvina C. Pessino, Marie Christine Combes, Cedric Mariac, Emidio Albertini, Fulvio Pupilli, Juan Pablo A. Ortiz, Olivier Leblanc
Published in: Scientific Data, Issue 11, 2024, ISSN 2052-4463
Publisher: Springer Science and Business Media LLC
DOI: 10.1038/s41597-024-03731-0

Trends in Apomixis Research: The 10 Most Cited Research Articles Published in the Pregenomic and Genomic Eras (opens in new window)

Author(s): Palumbo, Fabio; Draga, Samela; Vannozzi, Alessandro; Lucchin, Margherita; Barcaccia, Gianni
Published in: Frontiers in Plant Science, Issue 13, 2022, ISSN 1664-462X
Publisher: Frontiers Media S. A.
DOI: 10.3389/fpls.2022.878074

New insights into Eragrostis curvula’s sexual and apomictic reproductive development (opens in new window)

Author(s): María Cielo Pasten, José Carballo, Alejandra Raquel Díaz, Chiara Mizzotti, Mara Cucinotta, Lucia Colombo, Viviana Carmen Echenique, Marta Adelina Mendes
Published in: Frontiers in Plant Science, Issue 16, 2025, ISSN 1664-462X
Publisher: Frontiers Media S. A.
DOI: 10.3389/fpls.2025.1530855

Hormone immunolabeling in resin-embedded Arabidopsis tissues (opens in new window)

Author(s): Herrera-Ubaldo, Humberto; Nájera-González, Héctor-Rogelio; Luna-García, Valentín; Marsch-Martínez, Nayelli; de Folter, Stefan
Published in: STAR protocols, Issue 4, 2023, ISSN 2666-1667
Publisher: Cell Press Elsevier Inc.
DOI: 10.1016/j.xpro.2023.102514

TRIMETHYLGUANOSINE SYNTHASE1 mutations decanalize female germline development in Arabidopsis (opens in new window)

Author(s): Lorena A. Siena; Caroline Michaud; Benjamin Selles; Juan Manuel Vega; Silvina C. Pessino; Mathieu Ingouff; Juan Pablo A. Ortiz; Olivier Leblanc
Published in: New Phytologist, Issue 240, 2023, ISSN 0028-646X
Publisher: Blackwell Publishing Inc.
DOI: 10.1111/nph.19179

From tetraploid to diploid, a pangenomic approach to identify genes lost during synthetic diploidization of Eragrostis curvula (opens in new window)

Author(s): Jose Carballo; Andrés Martin Bellido; Juan Pablo Selva; Diego Zappacosta; Cristian Andres Gallo; Emidio Albertini; Mario Caccamo; Viviana Echenique
Published in: Frontiers in Plant Science, Issue 14, 2023, ISSN 1664-462X
Publisher: Frontiers Media S. A.
DOI: 10.3389/fpls.2023.1133986

Spotting the Targets of the Apospory Controller TGS1 in Paspalum notatum (opens in new window)

Author(s): Carolina Marta Colono; Maricel Podio; Lorena Adelina Siena; Juan Pablo A. Ortiz; Olivier Leblanc; Silvina Claudia Pessino
Published in: Plants, Issue 11, 2022, ISSN 2223-7747
Publisher: MDPI AG
DOI: 10.3390/plants11151929

<i>ORIGIN OF RECOGNITION COMPLEX 3</i> controls the development of maternal excess endosperm in the <i>Paspalum simplex</i> agamic complex (<i>Poaceae</i>) (opens in new window)

Author(s): Michele Bellucci; Maria Eugenia Caceres; Francesco Paolocci; Juan Manuel Vega; Juan Pablo Amelio Ortiz; Marilena Ceccarelli; Francesca De Marchis; Fulvio Pupilli
Published in: Journal of Experimental Botany, Issue 74, 2023, ISSN 0022-0957
Publisher: Oxford University Press
DOI: 10.1093/jxb/erad069

3D cellular morphometrics of ovule primordium development in Zea mays reveal differential division and growth dynamics specifying megaspore mother cell singleness (opens in new window)

Author(s): Ouedraogo, Inès; Lartaud, Marc; Baroux, Célia; Mosca, Gabriella; Delgado, Luciana; Leblanc, Oliver; Verdeil, Jean‐luc; Conéjéro, Geneviève; Autran, Daphné
Published in: Frontiers in Plant Science, Issue 14, 2023, ISSN 1664-462X
Publisher: Frontiers Media S. A.
DOI: 10.3389/fpls.2023.1174171

Proceedings of the 7th Series of Seminars on Advances in Apomixis Research (opens in new window)

Author(s): Autran, Daphné; Echenique, Viviana; Leblanc, Olivier
Published in: Plants, Issue 10(3), 2021, Page(s) 565, ISSN 2223-7747
Publisher: MDPI AG
DOI: 10.3390/plants10030565

Diploid aposporous sunflower forms triploid BIII progeny displaying increased apospory levels and non-random genetic mutations (opens in new window)

Author(s): Silvina Pessino, Graciela Nestares, Marta B. Bianchi, Iara Katzaroff, Lucía Amato, Marika Bocchini, Gianpiero Marconi, Emidio Albertini, Ana C. Ochogavía
Published in: Scientific Reports, Issue 15, 2025, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-025-89105-x

Gynoecium and fruit development in Arabidopsis (opens in new window)

Author(s): Herrera-Ubaldo, Humberto; de Folter, Stefan
Published in: Development, Issue 149(5), 2022, ISSN 1477-9129
Publisher: The Company of Biologists
DOI: 10.1242/dev.200120

The Auxin-Response Repressor IAA30 Is Down-Regulated in Reproductive Tissues of Apomictic Paspalum notatum (opens in new window)

Author(s): Siena,Lorena; Azzaro, Celeste; Podio, Maricel; Stein, Juliana; Leblanc, Olivier; Pessino, Silvina; Ortiz, Juan Pablo
Published in: Plants, Issue 11, 2022, ISSN 2223-7747
Publisher: MDPI AG
DOI: 10.3390/plants11111472

Eragrostis curvula, a Model Species for Diplosporous Apomixis (opens in new window)

Author(s): José Carballo; D. Zappacosta; D. Zappacosta; Juan Pablo Selva; Juan Pablo Selva; Mario Caccamo; Viviana Echenique; Viviana Echenique
Published in: Plants, Issue Vol. 10, 2021, ISSN 2223-7747
Publisher: Plants
DOI: 10.3390/plants10091818

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