Mechanisms of Apomictic Developments

Informazioni relative al progetto

MAD

ID dell’accordo di sovvenzione: 872417

DOI

10.3030/872417

Progetto chiuso

Data della firma CE 30 Gennaio 2020

Data di avvio 1 Dicembre 2020

Data di completamento 30 Novembre 2025

Finanziato da

• EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions

Costo totale

€ 1 035 000,00

Contributo UE

€ 952 200,00

Coordinato da

INSTITUT DE RECHERCHE POUR LE DEVELOPPEMENT
France

CORDIS fornisce collegamenti ai risultati finali pubblici e alle pubblicazioni dei progetti ORIZZONTE.

I link ai risultati e alle pubblicazioni dei progetti del 7° PQ, così come i link ad alcuni tipi di risultati specifici come dataset e software, sono recuperati dinamicamente da .OpenAIRE .

Risultati finali

Documents, reports (20)

List of candidate genes validated across several apomictic species by using the transcriptomic data available from previous projects
List of Arabidopsis mutants affected in AUX and/or CK pathways and defective in MMC fate
Characterisation of MMC fate, organ-level and cellular growth phenotypes in Arabidopsis transgenic lines perturbing cell wall composition in specific domains of the ovule

Report on the characterisation of MMC fate, organ-level and cellular growth phenotypes in Arabidopsis transgenic lines perturbing cell wall composition in specific domains of the ovule

Quantification of cell proliferation in the selected variants (P. rufum, maize, Arabidopsis) and their respective controls

Report on the quantification of cell proliferation in the selected variants (P. rufum, maize, Arabidopsis) and their respective controls

List of rice/maize/Paspalum mutants obtained through CRISPR/Cas

List of rice/maize/Paspalum mutants obtained through CRISPR/Cas for genes selected

Quantification of anisotropy of cell growth, cell wall changes in the selected variants (P. rufum, maize, Arabidopsis) and their respective controls

Report on the quantification of anisotropy of cell growth, cell wall changes in the selected variants (P. rufum, maize, Arabidopsis) and their respective controls

List of genes producing alternative splice variants in apomictic plants compared to sexual plants
List of mutants/genetically modified lines with repressed or enhanced activity of genes involved in the sexuality-apomixis switch
Characterization of reproductive phenotypes observed in Paspalum spp. and E. curvula transgenic lines carrying upregulated/downregulated candidate genes for apomixis

Reprot on the characterization of reproductive phenotypes observed in Paspalum spp. and E. curvula transgenic lines carrying upregulated/downregulated candidate genes for apomixis

3D cellular atlas of early ovules in the selected variants (P. rufum, maize, Arabidopsis), and their respective controls
List of genes showing alterations in post-transcriptional splicing and functional analysis of selected candidates
Epigenome landscapes in floral tissues of apomictic and sexual Paspalum and Eragrostis, including candidates under epigenetic control

Report on epigenome landscapes in floral tissues of apomictic and sexual Paspalum and Eragrostis, including candidates under epigenetic control

CRISP/cas9 genome editing in maize/rice

Reprot on CRISP/cas9 genome editing in maize/rice

ACL homoelogous regions in sexual grass genomes and list of candidate genes

Report for ACL homoelogous regions in sexual grass genomes and list of candidate genes

Delineate the structure and evolution of the ACL of Paspalum spp. and E. curvula and propose functional roles of genes located in it

Report on both structure and evolution of the ACL of Paspalum spp. and E. curvula and hypothesis for the functional roles of genes located in it

Ploidy analysis of the obtained mutants with apomictic phenotypes

Report on ploidy analysis of the obtained mutants with apomictic phenotypes

Biosensors analysis for AUX and CK signaling during the first stages of ovule development in P. notatum

Report for biosensors analysis for AUX and CK signaling during the first stages of ovule development in P. notatum

mRNA ISH expression patterns of known MMC genes to identify female germline markers in P. rufum and maize

Report on mRNA ISH expression patterns of known MMC genes to identify female germline markers in P. rufum and maize

Quantification of AUX and CK levels in sexual and apomictic ovules

Report on the quantification of AUX and CK levels in sexual and apomictic ovules

List of regulatory regions in megasporogenesis, megagametogenesis and embryo patterning genes showing deposition of epigenetic marks involved in their transcriptional or post-transcriptional regulation

Other (3)

MAD logo

deliver MAD logo

Kick-off meeting

Consortium general meetings

Workshops

Workshop

Websites, patent fillings, videos etc. (2)

Website

Web site scientific section

Video of MAD achievements

Short presentation video of MAD Consortium main achievements

Pubblicazioni

Peer reviewed articles (18)

A procedure for Dex-induced gene transactivation in Arabidopsis ovules

Autori: Schubert, Jasmin; Li, Yanru; Mendes, Marta A; Fei, Danli; Dickinson, Hugh; Moore, Ian; Baroux, Célia
Pubblicato in: Plant Methods, Numero 18, 2022, ISSN 1746-4811
Editore: BioMed Central
DOI: 10.1186/s13007-022-00879-x

Auxin response factor 10 insensitive to miR160regulation induces apospory-like phenotypes in Arabidopsis

Autori: Silvina Pessino, Mara Cucinotta, Carolina Colono, Elena Costantini, Davide Perrone, Maurizio Di Marzo, Giada Callizaya Terceros, Rosanna Petrella, Chiara Mizzotti, Celeste Azzaro, Maricel Podio, Gianpiero Marconi, Emidio Albertini, Hugh Dickinson, Lucia Colombo, Marta A. Mendes
Pubblicato in: iScience, Numero 27, 2025, Pagina/e 111115, ISSN 2589-0042
Editore: Elsevier BV
DOI: 10.1016/j.isci.2024.111115

Differentially methylated genes involved in reproduction and ploidy levels in recent diploidized and tetraploidized Eragrostis curvula genotypes

Autori: J. Carballo, A. Achilli, F. Hernández, M. Bocchini, M. C. Pasten, G. Marconi, E. Albertini, D. Zappacosta, V. Echenique
Pubblicato in: Plant Reproduction, Numero 37, 2024, Pagina/e 133-145, ISSN 2194-7953
Editore: Springer Verlag
DOI: 10.1007/s00497-023-00490-7

Transcriptome-guided breeding for Paspalum notatum: producing apomictic hybrids with enhanced omega-3 content

Autori: Lara Marino, Silvia Altabe, Carolina Marta Colono, Maricel Podio, Juan Pablo Amelio Ortiz, David Balaban, Juliana Stein, Nicolás Spoto, Carlos Acuña, Lorena Adelina Siena, José Gerde, Emidio Albertini, Silvina Claudia Pessino
Pubblicato in: Theoretical and Applied Genetics, Numero 138, 2025, ISSN 0040-5752
Editore: Springer Verlag
DOI: 10.1007/s00122-024-04788-6

Chromosome-scale genome assembly and annotation of Paspalum notatum Flüggé var. saurae

Autori: Juan Manuel Vega, Maricel Podio, Julie Orjuela, Lorena A. Siena, Silvina C. Pessino, Marie Christine Combes, Cedric Mariac, Emidio Albertini, Fulvio Pupilli, Juan Pablo A. Ortiz, Olivier Leblanc
Pubblicato in: Scientific Data, Numero 11, 2024, ISSN 2052-4463
Editore: Springer Science and Business Media LLC
DOI: 10.1038/s41597-024-03731-0

Trends in Apomixis Research: The 10 Most Cited Research Articles Published in the Pregenomic and Genomic Eras

Autori: Palumbo, Fabio; Draga, Samela; Vannozzi, Alessandro; Lucchin, Margherita; Barcaccia, Gianni
Pubblicato in: Frontiers in Plant Science, Numero 13, 2022, ISSN 1664-462X
Editore: Frontiers Media S. A.
DOI: 10.3389/fpls.2022.878074

New insights into Eragrostis curvula’s sexual and apomictic reproductive development

Autori: María Cielo Pasten, José Carballo, Alejandra Raquel Díaz, Chiara Mizzotti, Mara Cucinotta, Lucia Colombo, Viviana Carmen Echenique, Marta Adelina Mendes
Pubblicato in: Frontiers in Plant Science, Numero 16, 2025, ISSN 1664-462X
Editore: Frontiers Media S. A.
DOI: 10.3389/fpls.2025.1530855

Hormone immunolabeling in resin-embedded Arabidopsis tissues

Autori: Herrera-Ubaldo, Humberto; Nájera-González, Héctor-Rogelio; Luna-García, Valentín; Marsch-Martínez, Nayelli; de Folter, Stefan
Pubblicato in: STAR protocols, Numero 4, 2023, ISSN 2666-1667
Editore: Cell Press Elsevier Inc.
DOI: 10.1016/j.xpro.2023.102514

TRIMETHYLGUANOSINE SYNTHASE1 mutations decanalize female germline development in Arabidopsis

Autori: Lorena A. Siena; Caroline Michaud; Benjamin Selles; Juan Manuel Vega; Silvina C. Pessino; Mathieu Ingouff; Juan Pablo A. Ortiz; Olivier Leblanc
Pubblicato in: New Phytologist, Numero 240, 2023, ISSN 0028-646X
Editore: Blackwell Publishing Inc.
DOI: 10.1111/nph.19179

From tetraploid to diploid, a pangenomic approach to identify genes lost during synthetic diploidization of Eragrostis curvula

Autori: Jose Carballo; Andrés Martin Bellido; Juan Pablo Selva; Diego Zappacosta; Cristian Andres Gallo; Emidio Albertini; Mario Caccamo; Viviana Echenique
Pubblicato in: Frontiers in Plant Science, Numero 14, 2023, ISSN 1664-462X
Editore: Frontiers Media S. A.
DOI: 10.3389/fpls.2023.1133986

Spotting the Targets of the Apospory Controller TGS1 in Paspalum notatum

Autori: Carolina Marta Colono; Maricel Podio; Lorena Adelina Siena; Juan Pablo A. Ortiz; Olivier Leblanc; Silvina Claudia Pessino
Pubblicato in: Plants, Numero 11, 2022, ISSN 2223-7747
Editore: MDPI AG
DOI: 10.3390/plants11151929

<i>ORIGIN OF RECOGNITION COMPLEX 3</i> controls the development of maternal excess endosperm in the <i>Paspalum simplex</i> agamic complex (<i>Poaceae</i>)

Autori: Michele Bellucci; Maria Eugenia Caceres; Francesco Paolocci; Juan Manuel Vega; Juan Pablo Amelio Ortiz; Marilena Ceccarelli; Francesca De Marchis; Fulvio Pupilli
Pubblicato in: Journal of Experimental Botany, Numero 74, 2023, ISSN 0022-0957
Editore: Oxford University Press
DOI: 10.1093/jxb/erad069

3D cellular morphometrics of ovule primordium development in Zea mays reveal differential division and growth dynamics specifying megaspore mother cell singleness

Autori: Ouedraogo, Inès; Lartaud, Marc; Baroux, Célia; Mosca, Gabriella; Delgado, Luciana; Leblanc, Oliver; Verdeil, Jean‐luc; Conéjéro, Geneviève; Autran, Daphné
Pubblicato in: Frontiers in Plant Science, Numero 14, 2023, ISSN 1664-462X
Editore: Frontiers Media S. A.
DOI: 10.3389/fpls.2023.1174171

Proceedings of the 7th Series of Seminars on Advances in Apomixis Research

Autori: Autran, Daphné; Echenique, Viviana; Leblanc, Olivier
Pubblicato in: Plants, Numero 10(3), 2021, Pagina/e 565, ISSN 2223-7747
Editore: MDPI AG
DOI: 10.3390/plants10030565

Diploid aposporous sunflower forms triploid BIII progeny displaying increased apospory levels and non-random genetic mutations

Autori: Silvina Pessino, Graciela Nestares, Marta B. Bianchi, Iara Katzaroff, Lucía Amato, Marika Bocchini, Gianpiero Marconi, Emidio Albertini, Ana C. Ochogavía
Pubblicato in: Scientific Reports, Numero 15, 2025, ISSN 2045-2322
Editore: Nature Publishing Group
DOI: 10.1038/s41598-025-89105-x

Gynoecium and fruit development in Arabidopsis

Autori: Herrera-Ubaldo, Humberto; de Folter, Stefan
Pubblicato in: Development, Numero 149(5), 2022, ISSN 1477-9129
Editore: The Company of Biologists
DOI: 10.1242/dev.200120

The Auxin-Response Repressor IAA30 Is Down-Regulated in Reproductive Tissues of Apomictic Paspalum notatum

Autori: Siena,Lorena; Azzaro, Celeste; Podio, Maricel; Stein, Juliana; Leblanc, Olivier; Pessino, Silvina; Ortiz, Juan Pablo
Pubblicato in: Plants, Numero 11, 2022, ISSN 2223-7747
Editore: MDPI AG
DOI: 10.3390/plants11111472

Eragrostis curvula, a Model Species for Diplosporous Apomixis

Autori: José Carballo; D. Zappacosta; D. Zappacosta; Juan Pablo Selva; Juan Pablo Selva; Mario Caccamo; Viviana Echenique; Viviana Echenique
Pubblicato in: Plants, Numero Vol. 10, 2021, ISSN 2223-7747
Editore: Plants
DOI: 10.3390/plants10091818