Mechanisms of Apomictic Developments

Projektinformationen

MAD

ID Finanzhilfevereinbarung: 872417

DOI

10.3030/872417

Projekt abgeschlossen

EK-Unterschriftsdatum 30 Januar 2020

Startdatum 1 Dezember 2020

Enddatum 30 November 2025

Finanziert unter

• EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions

Gesamtkosten

€ 1 035 000,00

EU-Beitrag

€ 952 200,00

Koordiniert durch

INSTITUT DE RECHERCHE POUR LE DEVELOPPEMENT
France

CORDIS bietet Links zu öffentlichen Ergebnissen und Veröffentlichungen von HORIZONT-Projekten.

Links zu Ergebnissen und Veröffentlichungen von RP7-Projekten sowie Links zu einigen Typen spezifischer Ergebnisse wie Datensätzen und Software werden dynamisch von OpenAIRE abgerufen.

Leistungen

Documents, reports (20)

List of candidate genes validated across several apomictic species by using the transcriptomic data available from previous projects
List of Arabidopsis mutants affected in AUX and/or CK pathways and defective in MMC fate
Characterisation of MMC fate, organ-level and cellular growth phenotypes in Arabidopsis transgenic lines perturbing cell wall composition in specific domains of the ovule

Report on the characterisation of MMC fate, organ-level and cellular growth phenotypes in Arabidopsis transgenic lines perturbing cell wall composition in specific domains of the ovule

Quantification of cell proliferation in the selected variants (P. rufum, maize, Arabidopsis) and their respective controls

Report on the quantification of cell proliferation in the selected variants (P. rufum, maize, Arabidopsis) and their respective controls

List of rice/maize/Paspalum mutants obtained through CRISPR/Cas

List of rice/maize/Paspalum mutants obtained through CRISPR/Cas for genes selected

Quantification of anisotropy of cell growth, cell wall changes in the selected variants (P. rufum, maize, Arabidopsis) and their respective controls

Report on the quantification of anisotropy of cell growth, cell wall changes in the selected variants (P. rufum, maize, Arabidopsis) and their respective controls

List of genes producing alternative splice variants in apomictic plants compared to sexual plants
List of mutants/genetically modified lines with repressed or enhanced activity of genes involved in the sexuality-apomixis switch
Characterization of reproductive phenotypes observed in Paspalum spp. and E. curvula transgenic lines carrying upregulated/downregulated candidate genes for apomixis

Reprot on the characterization of reproductive phenotypes observed in Paspalum spp. and E. curvula transgenic lines carrying upregulated/downregulated candidate genes for apomixis

3D cellular atlas of early ovules in the selected variants (P. rufum, maize, Arabidopsis), and their respective controls
List of genes showing alterations in post-transcriptional splicing and functional analysis of selected candidates
Epigenome landscapes in floral tissues of apomictic and sexual Paspalum and Eragrostis, including candidates under epigenetic control

Report on epigenome landscapes in floral tissues of apomictic and sexual Paspalum and Eragrostis, including candidates under epigenetic control

CRISP/cas9 genome editing in maize/rice

Reprot on CRISP/cas9 genome editing in maize/rice

ACL homoelogous regions in sexual grass genomes and list of candidate genes

Report for ACL homoelogous regions in sexual grass genomes and list of candidate genes

Delineate the structure and evolution of the ACL of Paspalum spp. and E. curvula and propose functional roles of genes located in it

Report on both structure and evolution of the ACL of Paspalum spp. and E. curvula and hypothesis for the functional roles of genes located in it

Ploidy analysis of the obtained mutants with apomictic phenotypes

Report on ploidy analysis of the obtained mutants with apomictic phenotypes

Biosensors analysis for AUX and CK signaling during the first stages of ovule development in P. notatum

Report for biosensors analysis for AUX and CK signaling during the first stages of ovule development in P. notatum

mRNA ISH expression patterns of known MMC genes to identify female germline markers in P. rufum and maize

Report on mRNA ISH expression patterns of known MMC genes to identify female germline markers in P. rufum and maize

Quantification of AUX and CK levels in sexual and apomictic ovules

Report on the quantification of AUX and CK levels in sexual and apomictic ovules

List of regulatory regions in megasporogenesis, megagametogenesis and embryo patterning genes showing deposition of epigenetic marks involved in their transcriptional or post-transcriptional regulation

Other (3)

MAD logo

deliver MAD logo

Kick-off meeting

Consortium general meetings

Workshops

Workshop

Websites, patent fillings, videos etc. (2)

Website

Web site scientific section

Video of MAD achievements

Short presentation video of MAD Consortium main achievements

Veröffentlichungen

Peer reviewed articles (18)

A procedure for Dex-induced gene transactivation in Arabidopsis ovules

Autoren: Schubert, Jasmin; Li, Yanru; Mendes, Marta A; Fei, Danli; Dickinson, Hugh; Moore, Ian; Baroux, Célia
Veröffentlicht in: Plant Methods, Ausgabe 18, 2022, ISSN 1746-4811
Herausgeber: BioMed Central
DOI: 10.1186/s13007-022-00879-x

Auxin response factor 10 insensitive to miR160regulation induces apospory-like phenotypes in Arabidopsis

Autoren: Silvina Pessino, Mara Cucinotta, Carolina Colono, Elena Costantini, Davide Perrone, Maurizio Di Marzo, Giada Callizaya Terceros, Rosanna Petrella, Chiara Mizzotti, Celeste Azzaro, Maricel Podio, Gianpiero Marconi, Emidio Albertini, Hugh Dickinson, Lucia Colombo, Marta A. Mendes
Veröffentlicht in: iScience, Ausgabe 27, 2025, Seite(n) 111115, ISSN 2589-0042
Herausgeber: Elsevier BV
DOI: 10.1016/j.isci.2024.111115

Differentially methylated genes involved in reproduction and ploidy levels in recent diploidized and tetraploidized Eragrostis curvula genotypes

Autoren: J. Carballo, A. Achilli, F. Hernández, M. Bocchini, M. C. Pasten, G. Marconi, E. Albertini, D. Zappacosta, V. Echenique
Veröffentlicht in: Plant Reproduction, Ausgabe 37, 2024, Seite(n) 133-145, ISSN 2194-7953
Herausgeber: Springer Verlag
DOI: 10.1007/s00497-023-00490-7

Transcriptome-guided breeding for Paspalum notatum: producing apomictic hybrids with enhanced omega-3 content

Autoren: Lara Marino, Silvia Altabe, Carolina Marta Colono, Maricel Podio, Juan Pablo Amelio Ortiz, David Balaban, Juliana Stein, Nicolás Spoto, Carlos Acuña, Lorena Adelina Siena, José Gerde, Emidio Albertini, Silvina Claudia Pessino
Veröffentlicht in: Theoretical and Applied Genetics, Ausgabe 138, 2025, ISSN 0040-5752
Herausgeber: Springer Verlag
DOI: 10.1007/s00122-024-04788-6

Chromosome-scale genome assembly and annotation of Paspalum notatum Flüggé var. saurae

Autoren: Juan Manuel Vega, Maricel Podio, Julie Orjuela, Lorena A. Siena, Silvina C. Pessino, Marie Christine Combes, Cedric Mariac, Emidio Albertini, Fulvio Pupilli, Juan Pablo A. Ortiz, Olivier Leblanc
Veröffentlicht in: Scientific Data, Ausgabe 11, 2024, ISSN 2052-4463
Herausgeber: Springer Science and Business Media LLC
DOI: 10.1038/s41597-024-03731-0

Trends in Apomixis Research: The 10 Most Cited Research Articles Published in the Pregenomic and Genomic Eras

Autoren: Palumbo, Fabio; Draga, Samela; Vannozzi, Alessandro; Lucchin, Margherita; Barcaccia, Gianni
Veröffentlicht in: Frontiers in Plant Science, Ausgabe 13, 2022, ISSN 1664-462X
Herausgeber: Frontiers Media S. A.
DOI: 10.3389/fpls.2022.878074

New insights into Eragrostis curvula’s sexual and apomictic reproductive development

Autoren: María Cielo Pasten, José Carballo, Alejandra Raquel Díaz, Chiara Mizzotti, Mara Cucinotta, Lucia Colombo, Viviana Carmen Echenique, Marta Adelina Mendes
Veröffentlicht in: Frontiers in Plant Science, Ausgabe 16, 2025, ISSN 1664-462X
Herausgeber: Frontiers Media S. A.
DOI: 10.3389/fpls.2025.1530855

Hormone immunolabeling in resin-embedded Arabidopsis tissues

Autoren: Herrera-Ubaldo, Humberto; Nájera-González, Héctor-Rogelio; Luna-García, Valentín; Marsch-Martínez, Nayelli; de Folter, Stefan
Veröffentlicht in: STAR protocols, Ausgabe 4, 2023, ISSN 2666-1667
Herausgeber: Cell Press Elsevier Inc.
DOI: 10.1016/j.xpro.2023.102514

TRIMETHYLGUANOSINE SYNTHASE1 mutations decanalize female germline development in Arabidopsis

Autoren: Lorena A. Siena; Caroline Michaud; Benjamin Selles; Juan Manuel Vega; Silvina C. Pessino; Mathieu Ingouff; Juan Pablo A. Ortiz; Olivier Leblanc
Veröffentlicht in: New Phytologist, Ausgabe 240, 2023, ISSN 0028-646X
Herausgeber: Blackwell Publishing Inc.
DOI: 10.1111/nph.19179

From tetraploid to diploid, a pangenomic approach to identify genes lost during synthetic diploidization of Eragrostis curvula

Autoren: Jose Carballo; Andrés Martin Bellido; Juan Pablo Selva; Diego Zappacosta; Cristian Andres Gallo; Emidio Albertini; Mario Caccamo; Viviana Echenique
Veröffentlicht in: Frontiers in Plant Science, Ausgabe 14, 2023, ISSN 1664-462X
Herausgeber: Frontiers Media S. A.
DOI: 10.3389/fpls.2023.1133986

Spotting the Targets of the Apospory Controller TGS1 in Paspalum notatum

Autoren: Carolina Marta Colono; Maricel Podio; Lorena Adelina Siena; Juan Pablo A. Ortiz; Olivier Leblanc; Silvina Claudia Pessino
Veröffentlicht in: Plants, Ausgabe 11, 2022, ISSN 2223-7747
Herausgeber: MDPI AG
DOI: 10.3390/plants11151929

<i>ORIGIN OF RECOGNITION COMPLEX 3</i> controls the development of maternal excess endosperm in the <i>Paspalum simplex</i> agamic complex (<i>Poaceae</i>)

Autoren: Michele Bellucci; Maria Eugenia Caceres; Francesco Paolocci; Juan Manuel Vega; Juan Pablo Amelio Ortiz; Marilena Ceccarelli; Francesca De Marchis; Fulvio Pupilli
Veröffentlicht in: Journal of Experimental Botany, Ausgabe 74, 2023, ISSN 0022-0957
Herausgeber: Oxford University Press
DOI: 10.1093/jxb/erad069

3D cellular morphometrics of ovule primordium development in Zea mays reveal differential division and growth dynamics specifying megaspore mother cell singleness

Autoren: Ouedraogo, Inès; Lartaud, Marc; Baroux, Célia; Mosca, Gabriella; Delgado, Luciana; Leblanc, Oliver; Verdeil, Jean‐luc; Conéjéro, Geneviève; Autran, Daphné
Veröffentlicht in: Frontiers in Plant Science, Ausgabe 14, 2023, ISSN 1664-462X
Herausgeber: Frontiers Media S. A.
DOI: 10.3389/fpls.2023.1174171

Proceedings of the 7th Series of Seminars on Advances in Apomixis Research

Autoren: Autran, Daphné; Echenique, Viviana; Leblanc, Olivier
Veröffentlicht in: Plants, Ausgabe 10(3), 2021, Seite(n) 565, ISSN 2223-7747
Herausgeber: MDPI AG
DOI: 10.3390/plants10030565

Diploid aposporous sunflower forms triploid BIII progeny displaying increased apospory levels and non-random genetic mutations

Autoren: Silvina Pessino, Graciela Nestares, Marta B. Bianchi, Iara Katzaroff, Lucía Amato, Marika Bocchini, Gianpiero Marconi, Emidio Albertini, Ana C. Ochogavía
Veröffentlicht in: Scientific Reports, Ausgabe 15, 2025, ISSN 2045-2322
Herausgeber: Nature Publishing Group
DOI: 10.1038/s41598-025-89105-x

Gynoecium and fruit development in Arabidopsis

Autoren: Herrera-Ubaldo, Humberto; de Folter, Stefan
Veröffentlicht in: Development, Ausgabe 149(5), 2022, ISSN 1477-9129
Herausgeber: The Company of Biologists
DOI: 10.1242/dev.200120

The Auxin-Response Repressor IAA30 Is Down-Regulated in Reproductive Tissues of Apomictic Paspalum notatum

Autoren: Siena,Lorena; Azzaro, Celeste; Podio, Maricel; Stein, Juliana; Leblanc, Olivier; Pessino, Silvina; Ortiz, Juan Pablo
Veröffentlicht in: Plants, Ausgabe 11, 2022, ISSN 2223-7747
Herausgeber: MDPI AG
DOI: 10.3390/plants11111472

Eragrostis curvula, a Model Species for Diplosporous Apomixis

Autoren: José Carballo; D. Zappacosta; D. Zappacosta; Juan Pablo Selva; Juan Pablo Selva; Mario Caccamo; Viviana Echenique; Viviana Echenique
Veröffentlicht in: Plants, Ausgabe 10(9), 2021, Seite(n) 1818, ISSN 2223-7747
Herausgeber: MDPI AG
DOI: 10.3390/plants10091818