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Perfused Organs-on-a-Chip integrated with TEER measurement technique: A novel approach towards studying non-targeted drug induced organ toxicity

Project description

Microfluidic organs-on-a-chip device to improve preclinical drug development

The EU-funded PORTable STaNDOuTs project aims to develop organs-on-a-chip microfluidic devices integrated with electrodes for transepithelial electrical resistance (TEER) measurements to replace current time-consuming and cost-ineffective preclinical procedures in the drug development process. TEER measurement is rapid and non-invasive, and it indicates the integrity of the cell membrane barrier of different organs necessary for homeostasis in the human body. The objective is to fabricate a perfused device with three compartments, one for the intestine, one for the liver and one for the kidneys, independently equipped with electrodes for measuring TEER values to detect the integrity of cell membrane barriers in each organ compartment. The developed technology will be subsequently validated for its ability to study drug-induced nephrotoxicity.

Objective

PORTable STaNDOuTs aims to develop a multiple Organs-on-a-Chip (MOOC) microfluidic device integrated with electrodes for carrying out Transepithelial electrical resistance (TEER) measurements. The key motivation for adopting such an approach is to develop a rapid, versatile and cost-effective prototype with ability for real-time monitoring to replace the conventional long and cost-ineffective preclinical procedures involved in the drug development process. On administration of an oral pharmaceutical drug, major organs that are involved in the absorption and metabolism of drug are intestine and liver. The drug is then distributed to different target organs such as brain, heart, lung, etc. Finally, the drug metabolites are eliminated from the human system with the help of kidney. Additionally, it has also been found that certain drug metabolites produced by the liver cause non-target drug induced organ toxicity. TEER measurement is a rapid and non-invasive technique for the real-time detection of pathophysiology. TEER indicates the integrity of the cell membrane barrier of different organs that is necessary for maintaining homeostasis within the human body. In view of this, we intend to fabricate a physiologically-mimicking perfused intestine-liver-kidney microfluidic chip with provision for carrying out in-chip TEER measurements. The device will be designed to consist of three compartments for organs namely, intestine, liver and kidney, independently equipped with electrodes for measuring TEER values to detect the integrity of cell membrane barriers in each organ compartment. The developed device will be then subsequently validated for its ability to study drug-induced nephrotoxity. Furthermore, the device will also be made versatile to incorporate other organs of interest to study non-target organ toxicity. Thus, the developed MOOC microfluidic device will surely boost the drug development process that will successively improve the health security of the society.

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Keywords

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Programme(s)

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Topic(s)

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Funding Scheme

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MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF)

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Call for proposal

Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.

(opens in new window) H2020-MSCA-IF-2019

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Coordinator

CHERRY BIOTECH
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 184 707,84
Address
2EME ETAGE, HALLS A-B ET C14 RUE DE LA BEAUNE
93100 MONTREUIL
France

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SME

The organization defined itself as SME (small and medium-sized enterprise) at the time the Grant Agreement was signed.

Yes
Region
Ile-de-France Ile-de-France Seine-Saint-Denis
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

€ 184 707,84
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