Project description DEENESFRITPL Coming out of the darkness: understanding metabolic fluxes in phototrophic eukaryotes Most of us are familiar with circadian rhythms, oscillatory internal timing conforming to the 24-hour rotation of the Earth. Metabolic processes are strongly linked to them to optimise energy use across the light-dark cycle. Similarly, dark-light transitions are very important to phototrophs, which get their energy from sunlight through photosynthesis. Dark-light transitions provoke changes in the redox state of photosynthetic components that modulate metabolic fluxes. The EU-funded CHLARABIDOX project studies the proteome-wide dynamics in response to light in two phototrophic species (the green alga Chlamydomonas reinhardtii and the plant Arabidopsis thaliana). High temporal resolution of light-induced redox-related metabolic changes could help manipulate energy processes for biofuels production and identify modifications that help plants adjust to climate change. Show the project objective Hide the project objective Objective Most organisms exhibit a diurnal metabolic cycle, especially phototrophs, whose metabolism is strictly dependent on light. Dark-light transitions are accompanied by dramatic changes in the redox state of photosynthetic components, which drives redox-based post-translational modification of protein cysteines, whose oxidation state can considerably impact protein activity, and thus regulate metabolism. Given the central role of redox metabolism in biology, the operation of thiol-disulphide based switches are well-appreciated as a metabolic acclimation strategy, and the study of cysteine modifications in proteomes is a major interest of contemporary biology. The objective of CHLARABIDOX is to go beyond inventories of redox modified proteins by monitoring the proteome-wide dynamics of disulphide-dithiol status in the context of a diurnal metabolic cycle in phototrophic eukaryotes, specifically, the green alga Chlamydomonas reinhardtii and the land plant Arabidopsis thaliana. An innovative chemoproteomic isoTOP-ABPP approach will be used in an experimental design with deep temporal resolution to capture a good fraction of the proteome with site specificity and quantitative information about reactivity. The discoveries will be made in the context of a body of literature on thioredoxin-dependent redox regulation of central carbon metabolism, which will serve as a priori validation. The outcome of the project is a proteome-wide view of the operation of regulatory redox sensors, anchored to accompanying rich datasets on physiology, metabolic potential, transcriptomics, proteomics and central metabolites, which would inform the operation of light-driven metabolic networks. Both systems are compatible with downstream modelling of diurnal metabolic fluxes and validation by reverse genetics approaches. A long term impact on strategies for manipulating metabolism for biofuels production, or manipulating photosynthesis for better acclimation to climate change is also envisioned. Fields of science natural sciencesbiological sciencesbiochemistrybiomoleculesproteinsproteomicsnatural sciencesbiological sciencesmicrobiologyphycologynatural scienceschemical scienceselectrochemistryelectrolysisnatural sciencesearth and related environmental sciencesatmospheric sciencesclimatologyclimatic changesnatural sciencesbiological sciencesbotany Keywords Chlamydomonas reinhardtii Arabidopsis thaliana Redox proteomics Photosynthesis Systems biology of redox regulation thioredoxin Programme(s) H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions Main Programme H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility Topic(s) MSCA-IF-2019 - Individual Fellowships Call for proposal H2020-MSCA-IF-2019 See other projects for this call Funding Scheme MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF) Coordinator UNIVERSIDAD DE SEVILLA Net EU contribution € 263 732,16 Address CALLE S. FERNANDO 4 41004 Sevilla Spain See on map Region Sur Andalucía Sevilla Activity type Higher or Secondary Education Establishments Links Contact the organisation Opens in new window Website Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window Total cost € 263 732,16 Partners (1) Sort alphabetically Sort by Net EU contribution Expand all Collapse all Partner Partner organisations contribute to the implementation of the action, but do not sign the Grant Agreement. THE REGENTS OF THE UNIVERSITY OF CALIFORNIA United States Net EU contribution € 0,00 Address FRANKLIN STREET 1111 12 FLOOR 94607 OAKLAND CA See on map Activity type Higher or Secondary Education Establishments Links Contact the organisation Opens in new window Website Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window Total cost € 177 265,92
