Description du projet
Interaction vecteur-virus pour le syndrome de fièvre sévère avec thrombocytopénie transmis par les tiques
Les tiques transmettent de nombreux types d’arbovirus, dont le virus du syndrome de fièvre sévère avec thrombocytopénie (SFTSV), un phénovirus récemment découvert dans certains pays asiatiques. Les tiques Ripicephalus microplus sont les vecteurs du SFTSV mortel, et il n’existe pas de données génomiques de base pour ces arthropodes non modèles. Financé par le programme Actions Marie Skłodowska-Curie, le projet TICKITS a pour objectif de développer une approche innovante, en acquérant des données combinées de transcriptomique et de protéomique à partir des cellules de tiques lors de l’infection par le SFTSV. Cette étude complexe de la réponse de l’hôte fournira des informations sur des cibles potentielles qui pourront être étudiées plus en détail afin de déterminer leur impact sur l’infection virale.
Objectif
Among hematophagous arthropods ticks transmit a large variety of pathogens with public health and veterinary importance. Tick transmit several emergent and re-emergent arboviruses across the world. In 2010, scientists discovered severe fever with thrombocytopenia syndrome virus (SFTSV), an emergent Banyangvirus (Phenuivirus) transmitted by ticks in Asian countries. SFTSV infection causes severe symptoms and can lead to the death in infected individuals. Novel vector-based control strategies require a more detailed understanding of the vector-virus interaction and the vector response to infection.
Ripicephalius microplus ticks are vectors of SFTSV and as for many non-model arthropods we are missing basic genomic data, which makes tick-arbovirus interaction studies difficult. To overcome these limitations, the TICKITS (TICK cell Interactions with SFTSV) project aims to develop an innovative approach coupling transcriptomic and proteomics data from R. microplus cells during infection. This systematic approach will characterize SFTSV infections in their natural tick vector and will generate information on changes in vector cells both at transcriptome and proteome level. This integrative view of the host response will give us insights on targets that can be investigated using gene silencing approaches to determine their impact on viral infection. I choose to work with the Kohl and Brennan laboratories at the MRC-University of Glasgow Centre for Virus Research (CVR) in Glasgow, UK. Both laboratories excel in tick/arthropod biology and arbovirus infection studies and have all the tools necessary to carry out this work. By adding my molecular and omics expertise we aim to develop novel angles in tick research. Moreover, the data generated will provide a solid base for other projects to build on and develop comparative projects across species.
Champ scientifique
Programme(s)
Régime de financement
MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF)Coordinateur
G12 8QQ Glasgow
Royaume-Uni