Descrizione del progetto
Interazione vettore-virus nella febbre grave trasmessa da zecche con il virus della sindrome da trombocitopenia
Le zecche sono trasmettitori di molti tipi di arbovirus, che comprendono il virus recentemente scoperto della febbre grave con sindrome da trombocitopenia (SFTSV, severe fever with thrombocytopenia syndrome virus), un fenuivirus emergente in alcuni paesi asiatici. Le zecche Ripicphalus microplus fungono da vettori di SFTSV mortale e non si dispone di dati genomici di base per questi artropodi non-modello. Finanziato dal programma di azioni Marie Skłodowska-Curie, il progetto TICKITS è volto a sviluppare un approccio innovativo, acquisendo dati combinati di trascrittomica e proteomica dalle cellule delle zecche durante l’infezione da SFTSV. Questo complesso studio della risposta dell’ospite fornirà informazioni sui potenziali bersagli che possono essere ulteriormente studiati per determinare la loro incidenza sull’infezione virale.
Obiettivo
Among hematophagous arthropods ticks transmit a large variety of pathogens with public health and veterinary importance. Tick transmit several emergent and re-emergent arboviruses across the world. In 2010, scientists discovered severe fever with thrombocytopenia syndrome virus (SFTSV), an emergent Banyangvirus (Phenuivirus) transmitted by ticks in Asian countries. SFTSV infection causes severe symptoms and can lead to the death in infected individuals. Novel vector-based control strategies require a more detailed understanding of the vector-virus interaction and the vector response to infection.
Ripicephalius microplus ticks are vectors of SFTSV and as for many non-model arthropods we are missing basic genomic data, which makes tick-arbovirus interaction studies difficult. To overcome these limitations, the TICKITS (TICK cell Interactions with SFTSV) project aims to develop an innovative approach coupling transcriptomic and proteomics data from R. microplus cells during infection. This systematic approach will characterize SFTSV infections in their natural tick vector and will generate information on changes in vector cells both at transcriptome and proteome level. This integrative view of the host response will give us insights on targets that can be investigated using gene silencing approaches to determine their impact on viral infection. I choose to work with the Kohl and Brennan laboratories at the MRC-University of Glasgow Centre for Virus Research (CVR) in Glasgow, UK. Both laboratories excel in tick/arthropod biology and arbovirus infection studies and have all the tools necessary to carry out this work. By adding my molecular and omics expertise we aim to develop novel angles in tick research. Moreover, the data generated will provide a solid base for other projects to build on and develop comparative projects across species.
Campo scientifico
Programma(i)
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Meccanismo di finanziamento
MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF)Coordinatore
G12 8QQ Glasgow
Regno Unito