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Host range and genome adaptation of giant viruses

Project description

Host range and genome adaptation of giant viruses

Giant viruses have unusually large genomes and encode translation-related genes, which means that they have more translational independence compared to other viruses. Although their discovery has challenged the existing perception on viruses, their host range and natural host species remain to be determined. The working hypothesis of the EU-funded GIVIREVOL project is that codon usage is critical for adaptation and viral fitness. Using silico and wet-lab strategies, scientists will analyse the codon usage preferences among giant viruses and predict the hosts they infect. These predictions will be experimentally verified. The project will also provide information on how genome adaptation shapes the evolutionary relationship between giant viruses and their hosts, advancing the field of evolutionary virology.

Objective

For more than a century, viruses were considered tiny particles, fully dependent on their host cells to replicate. The recent discovery of giant viruses, containing unusually large genomes, challenged this assumption and blurred the sharp division between viruses and cellular life. It was also striking to learn that many of the giant virus genomes encode translation-related genes, indicating that they are presumably more independent -in terms of translation- as compared to other viruses and potentially infect and replicate in a broad range of hosts. Nonetheless, for most of the giant viruses, the precise host range and natural host species remain to be investigated.
In the proposed project, I will investigate the host range and genome adaptation of giant viruses by combining in silico and wet-lab strategies. I postulate that, codon usage is an important factor in the adaptation of giant viruses to their hosts, where well-adapted codon usage provides for superior viral fitness. I will analyse the codon usage preferences among giant viruses and correlate these with the known and presumable hosts they infect. This will allow me to computationally predict the best-suited hosts, and subsequently, experimentally assess my predictions on different laboratory hosts. To further investigate whether codon usage defines the rate of genome adaptation of giant viruses, I will perform experimental evolution over a half year time period.
The origin and evolutionary history of giant viruses is controversially discussed. By investigating the evolutionary relationships between giant viruses and their hosts in the context of their codon usage preferences, I will contribute to a better understanding of the factors determining host range and the evolutionary processes shaping giant virus genomes. Disentangling the connection between genomic content and host range will provide important knowledge in the fields of virology, evolutionary biology, genomics, and virus-host interactions.

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MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF)

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Call for proposal

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(opens in new window) H2020-MSCA-IF-2019

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Coordinator

UNIVERSITAT WIEN
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 186 167,04
Address
UNIVERSITATSRING 1
1010 WIEN
Austria

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Region
Ostösterreich Wien Wien
Activity type
Higher or Secondary Education Establishments
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

€ 186 167,04
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