Functional high-throughput analysis of the role of microRNAs in regulating Staphylococcus aureus infection

Description du projet

Le rôle des microARN dans l’infection à Staphylococcus aureus

Les infections à Staphylococcus aureus (S. aureus) constituent une cause principale de morbidité et de mortalité dans les pays développés. En raison de la prévalence élevée de souches de S. aureus résistantes aux antibiotiques, il s’avère nécessaire de mieux comprendre les facteurs qui gouvernent l’interaction hôte-agent pathogène et provoquent la résistance bactérienne. De nouvelles preuves indiquent l’implication de microARN dans la résistance aux antibiotiques, l’interaction entre l’agent pathogène et l’hôte, ou comme mécanisme d’évasion immunitaire. Dans cette optique, les scientifiques du projet miRs4Staph, financé par l’UE, caractériseront les microARN hôtes impliqués dans l’interaction entre S. aureus et l’hôte. Par le biais de diverses stratégies innovantes et interdisciplinaires, le projet identifiera et validera des microARN impliqués dans l’infection à S. aureus qui pourraient servir de cibles pour la conception de futures interventions thérapeutiques.

Objectif

Staphylococcus aureus is an opportunistic bacterial pathogen that causes acute and chronic infections responsible for a broad spectrum of human diseases. The high prevalence of antibiotic-resistant strains in clinical context and the spreading of these strains in the community, make S. aureus infections a leading cause of morbidity and mortality in developed countries. A better understanding of the factors contributing to bacteria resistance and host-pathogen interaction is essential to counteract S. aureus infection and, ultimately, to develop more effective therapeutic approaches. MicroRNAs (miRNAs) are genome-encoded small non-coding RNAs, which play a pervasive role in the post-transcriptional control of eukaryotic gene expression. Emerging evidence supports a determinant role for miRNAs in the interplay between bacterial pathogens and host cells, as part of the immune response, or as a molecular strategy exploited by pathogens to subvert host cellular functions. However, a comprehensive analysis of host miRNAs function during infection by S. aureus is still missing.

The main goal of this project is to identify and characterize host miRNAs, which are critical for S. aureus-host interaction at the cellular level. This will be achieved through interdisciplinary and innovative strategies, such as large-scale genome-wide miRNAs screenings and high-content analysis. To increase the biological relevance of the study, the effect of selected miRNAs will be validated in S. aureus clinical isolates obtained from osteomyelitis patients. The identification and characterization of miRNA molecular targets will be based on computational and experimental approaches.

Through the application of cutting-edge techniques, this project will reveal previously unappreciated factors/pathways essential for S. aureus infection, providing insights into the complex host-pathogen interplay. Additionally, the identified novel factors/pathways may be explored for therapeutic intervention.