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Gene network rewiring by an engineered CRIPSR-Cas12a variant

Project information

Grant agreement ID: 894574

Status

Grant agreement signed

  • Start date

    1 December 2020

  • End date

    30 November 2022

Funded under:

H2020-EU.1.3.2.

  • Overall budget:

    € 171 473,28

  • EU contribution

    € 171 473,28

Coordinated by:

POLITECNICO DI TORINO

Italy

Objective

In the last 10 years, an increasing number of studies employed -omics technologies to describe the molecular changes underpinning cellular functions. However, a large part of these findings is awaiting an experimental validation. This is due to the lack of efficient molecular tools able to control both multiple and distinct genetic interactions. Recently, I described a new CRISPR/12-based genome engineering tool that, for the first time, it provides the constitutive, conditional, inducible, orthogonal and multiplexed engineering of dozens of endogenous genes, simultaneously. In this research proposal, I aim to increase the efficiency of this platform in the context of multiplexed genome engineering applications, such as gene network rewiring. To this aim, I will use techniques inspired by the statistical physics of complex disordered systems, to design a more potent version of my CRISPR/Cas12-based genome engineering tool and, I will use this novel platform to rewire signaling pathways involved in cellular proliferation. This research proposal is structured into the following tasks: rational design of novel Cas12a variants, validation of novel Cas12a variants, gene network rewiring by a novel Cas12a variant. By coupling statistical physics to both protein and genome engineering this project will pave the way for efficient and large-scale engineering of gene networks.
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Coordinator

POLITECNICO DI TORINO

Address

Corso Duca Degli Abruzzi 24
10129 Torino

Italy

Activity type

Higher or Secondary Education Establishments

EU Contribution

€ 171 473,28

Project information

Grant agreement ID: 894574

Status

Grant agreement signed

  • Start date

    1 December 2020

  • End date

    30 November 2022

Funded under:

H2020-EU.1.3.2.

  • Overall budget:

    € 171 473,28

  • EU contribution

    € 171 473,28

Coordinated by:

POLITECNICO DI TORINO

Italy