Project description
Molecular insight into the regulation of floral transition in plants
The process by which plants switch from vegetative to reproductive growth is known as floral transition. The flowering stimulus comes from the Flowering Locus T (FT) gene, which reprograms the transcription of floral identity genes, thereby inducing flowering. The EU-funded FD Net project aims to provide a deeper understanding of the floral transition process in the model plant Arabidopsis thaliana by focussing on the key transcription factor Flowering Locus D (FD) and its transcriptomic network. Investigation of the regulatory steps that control FD activity, including post-translational modifications, will help decipher FD function and provide fundamental knowledge on floral transition in plants.
Objective
Floral transition denotes the initiation of plant reproduction. Several genetic pathways regulate this important transition and many of them converge on the regulation of FLOWERING LOCUS T (FT), the systemic flowering signal that moves from leaves to the shoot apex. FT reprograms transcription at the shoot apex by contributing to Florigen Activation Complex (FAC). A central component of this complex is the bZIP transcription factor FLOWERING LOCUS D (FD), which is responsible for DNA binding and gene specific regulation by the complex. I will employ single-cell RNA sequencing to capture the transient cell state where the FAC complex and two different target genes, APETALA1 and SOC1, are coexpressed. Defining the transcriptomic network of FD at the single-cell level will provide a deeper understanding of this dynamic process. Phosphorylation of a threonine residue in the SAP motif at the C terminus of FD is a key step in formation of the complex. Phosphomimetic or non-phosphorylatable FD SAP motif versions have opposite effects on FD function, being able or not, respectively, to complement the late flowering phenotype of fd-3. I will investigate whether SAP motif phosphorylation affects FD protein stability or impairs its protein-protein interactions using confocal microscopy and protein mass spectrometry. In addition, other bZIPs closely related to FD are phosphorylated at other sites, but no analysis of FD phosphorylation sites other than the SAP motif has been presented. I will map post-translational modifications of FD, which may reveal new regulatory steps in controlling FD activity. I will explore the ability of FD to form protein complexes with other transcription factors by employing yeast-two hybrid, protein pull-downs and interaction studies using immunoprecipitation-mass spectrometry and biotin-based proximity labeling. The identification of new FD protein interactors will start reverse genetic and biochemical approaches to better understand FD function.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules proteins proteomics
- natural sciences biological sciences genetics DNA
- natural sciences biological sciences genetics RNA transcriptomes
- natural sciences physical sciences optics microscopy confocal microscopy
- natural sciences chemical sciences analytical chemistry mass spectrometry
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions
MAIN PROGRAMME
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H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility
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Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF)
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Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) H2020-MSCA-IF-2019
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Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
80539 MUNCHEN
Germany
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.