Structural and mechanistic study of co-translationally interacting nascent chains

An assembly of components put together at different stages leads to co-co assembly. What is more, co-co assembly is mostly employed for the formation of homo-oligomers and exists in all kingdoms of life. Still, very little is known about the molecular mechanisms that drive it. The EU-funded Co-coAssembly project will study mechanisms of co-co assembly using the dimeric chorismate mutase as a representative top candidate from a high throughput screen for co-co assembling protein complexes in E. coli. Employing special tomography, the study will focus on the three-dimensional arrangement of E. coli ribosomes to assess how the organisation of translational machinery allows co-co assembly.