Exploring the evolution of wild microbes using a molecular genomic approach

Projektinformationen

WildE

ID Finanzhilfevereinbarung: 895338

DOI

10.3030/895338

Projekt abgeschlossen

EK-Unterschriftsdatum 20 April 2020

Startdatum 1 September 2021

Enddatum 21 März 2024

Finanziert unter

• EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions

Gesamtkosten

€ 172 932,48

EU-Beitrag

€ 172 932,48

Koordiniert durch

AGENCIA ESTATAL CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS
Spain

Periodic Reporting for period 1 - WildE (Exploring the evolution of wild microbes using a molecular genomic approach)

Berichtszeitraum: 2021-09-01 bis 2023-08-31

Zusammenfassung vom Kontext und den Gesamtzielen des Projekts

Marine microbes are the foundation of all life in the oceans, yet our understanding of how they are reacting to global change is limited. These microbes typically exist in large populations and reproduce quickly, which might enable them to rapidly adapt to environmental stressors compared to larger organisms. However, there is limited genetic evidence of contemporary evolution in wild marine microbes.

Understanding how marine microbes evolve is crucial for society because they are essential to ocean life. We do not currently know how these microbes will adapt to future global changes and what the potential consequences will be for ocean ecosystems and the food chain. Changes in microbial traits, such as growth rates, cell size, chlorophyll content, and photosynthesis, could lead to shifts in bloom events, primary production, and species composition. This, in turn, could affect the biogeochemical cycles that sustain life in the ocean.

The overall objectives are to:
- Improve understanding of how marine microbes are evolving in response to global change.
- Establish long-term studies (time series) to track changes in ocean microbes.

Arbeit, die ab Beginn des Projekts bis zum Ende des durch den Bericht erfassten Berichtszeitraums geleistet wurde, und die wichtigsten bis dahin erzielten Ergebnisse

To achieve the goals of the WildE project, the research fellow designed a long-term mesocosm experiment to monitor changes in microbial populations. Temporal mesocosm experiments can take laboratory experiments a step closer to the complexity of the natural world, providing important insights into how whole communities might evolve and interact under future global change scenarios. This 14-month experiment used natural assemblages of microbes from a freshwater lake to replicate future global warming scenarios. Environmental DNA (eDNA) was sampled every two weeks and environmental RNA (eRNA) was collected during the last three weeks of the experiment. Both eDNA and eRNA were sequenced to track microbial evolution over time.

Additionally, the project utilised the Blanes Bay Microbial Observatory (BBMO) time series data, covering 2009-2020, to measure the rate of evolution in wild microbes. Metagenome assembled genomes (MAGs) were created from this data, and the most abundant MAGs were analysed. This analysis is ongoing, with a manuscript in preparation to quantify how environmental drivers influence microbial community assemblages.

The project also produced an invited opinion article in "Trends in Microbiology," highlighting the importance of understanding microbial evolution in response to global changes. Novel DNA and RNA data banks have been created and will be made publicly available, enabling further research and collaboration. The fellow has contributed to six co-author papers, one textbook chapter, and additional manuscripts in preparation, significantly advancing the understanding of microbial evolution.

Fortschritte, die über den aktuellen Stand der Technik hinausgehen und voraussichtliche potenzielle Auswirkungen (einschließlich der bis dato erzielten sozioökonomischen Auswirkungen und weiter gefassten gesellschaftlichen Auswirkungen des Projekts)

The mesocosm experiment represents a significant advancement by using natural assemblages instead of single isolates, providing a more accurate reflection of how wild populations respond to environmental changes. This approach allows for the disentanglement of specific temperature effects from other environmental drivers.

The project has generated two novel time-series datasets: a 12-year monthly sampling from the Mediterranean and a 14-month bi-weekly sampling from a mesocosm. These datasets contribute to a deeper understanding of microbial evolution in response to environmental drivers. Given the crucial roles of marine microbes in global carbon and nitrogen fixation, this research helps predict how future global changes might impact ecological services.

The sequence data will be accessible for future research, fostering collaborations and advancements in the field. The socio-economic impacts include better-informed policies for ecosystem management and climate change mitigation, benefiting both scientists and policymakers.

Illustration of the current state of knowledge and future goals in microbial evolution research.