Development of a novel continuous Extracellular Vesicle production system

Description du projet

Des méthodes avancées à l’échelle nanométrique pour des aliments sains

Les aliments fonctionnels peuvent être optimisés à l’aide de nutriments bioactifs améliorer la santé et atténuer les maladies. L’encapsulation peut également être utilisée pour faciliter l’absorption des composés difficilement assimilés. Des méthodes innovantes à l’échelle nanométrique permettent de créer des options alternatives d’administration des produits nutraceutiques. Le projet greenEV, financé par l’UE, propose le développement d’un système de culture tissulaire continue issue de plantes pour la fabrication de nanovésicules non mammifères, qui serviront dans le cadre de l’encapsulation, de la délivrance et de l’absorption de la biodisponibilité des produits nutraceutiques sélectionnés. Les vésicules végétales sont morphologiquement identiques aux vésicules extracellulaires mammifères (VE). Le projet va mettre en place un système innovant de fabrication de VE végétales. Il utilisera également un pipeline analytique intégré pour l’isolation, la caractérisation, l’encapsulation, la consommation et la description toxicologique des VE sécrétées par les plantes.

Objectif

Functional foods boosted with bioactive nutrients to enhance health and mitigating disease conditions are experiencing an amazing progress. Encapsulation is becoming an important strategy to enhance the bioavailability of poorly absorbed compounds and developing healthy foods. In this context, novel nano-scale approaches to create alternative delivery options for nutraceuticals are emerging. GreenEV aims to develop a continuous plant tissue culturing system for the manufacturing of non-mammalian nanovesicles, and exploit them for the encapsulation, release and bioavailability enhancement of selected nutraceuticals. Plants cells have been shown to physiologically release phospholipid membrane-surrounded vesicles into the apoplast and environment. These vesicles are morphologically similar to mammalian extracellular vesicles (EVs). The process that drive the vesicle formation and their release through the semi-rigid cell wall is not trivial, and will be studied in the greenEV.

The experienced researcher is a plant biologist with strong background in plant tissue culturing who team-up with the Institute of Biosciences and BioResources (IBBR) with considerable expertise in EV research to realize a uniquely interdisciplinary research program. The research proposed here will realize the following concrete objectives: 1. Set-up a novel, plant-based EV production system, 2. Employ an integrated analytical pipeline for the isolation, characterization, encapsulation, uptake and toxicological profiling of plant secreted EVs. Neither of these objectives have been addressed before and both have high potential to expand the knowledge in the field and to drive the research activity towards industrialized continuous production of plant EVs. The research objectives are integrated with concerted training objectives in EV research, omics, outreach program, dissemination events and considerable knowledge transfer in plant tissue culturing from the researcher to the IBBR host group.