Precisely Patterned Nanofibers for High Performance Bioseparations

Información del proyecto

PURE

Identificador del acuerdo de subvención: 899732

DOI

10.3030/899732

Proyecto cerrado

Fecha de la firma de la CE 26 Mayo 2020

Fecha de inicio 1 Octubre 2020

Fecha de finalización 31 Marzo 2025

Financiado con arreglo a

• EXCELLENT SCIENCE - Future and Emerging Technologies (FET)

Coste total

€ 2 999 537,50

Aportación de la UE

€ 2 999 537,50

Coordinado por

UNIVERSIDADE NOVA DE LISBOA
Portugal

CORDIS proporciona enlaces a los documentos públicos y las publicaciones de los proyectos de los programas marco HORIZONTE.

Los enlaces a los documentos y las publicaciones de los proyectos del Séptimo Programa Marco, así como los enlaces a algunos tipos de resultados específicos, como conjuntos de datos y «software», se obtienen dinámicamente de OpenAIRE .

Resultado final

Other (4)

Bioproduction of non-canonical amino acids

At least 500 mg of reactive ncAA AhA will be produced by biotechnological means

Spidroin models

Unmodified, labeled and ligand modified spidroin (in solution and in fibrils) will be analyzed by SAXS (BOKU) and then modeled by NOVA.At least 10 multiscale models of modified spidroin are expected.

Bioproduction of unmodified spidroins

At least 500 mg of unmodified spidroins will be biologically produced

Production of activated affinity ligands

At least 250 mg of each activated affinity ligand will be attained The affinity ligands selective for factor VIII Mabs VLPs and protein A will be produced and modified with DBCO for further modification of labeled spidroin

Open Research Data Pilot (1)

Data Management Plan

Data Management PlanA detailed Data Management Plan will be created at the beginning of the project to ensure that all data is findable accessible interoperable and reusable FAIR The plan will be harmonized with a knowledge protection plan IPR plan

Websites, patent fillings, videos etc. (2)

Future exploitation

Collaborative framework model & business plan with list of stakeholders for future exploitation

PURE webportal operational

Documents, reports (1)

Dissemination and Exploitation Plan

Dissemination and Exploitation Plan at the end of the first Reporting Period RP1 M12

Publicaciones

Peer reviewed articles (11)

Heparin-affinity chromatography is a generic purification platform for chimeric gag VLPs displaying different viral surface antigens

Autores: Alexander M. Zollner, Leticia Guzman Ruiz, Viktoria Mayer, Stefanie Stohl, Leo A. Jakob, Nico Lingg, Miriam Klausberger, Alois Jungbauer, Patricia Pereira Aguilar
Publicado en: Separation and Purification Technology, Edición 340, 2024, Página(s) 126673, ISSN 1383-5866
Editor: Pergamon Press Ltd.
DOI: 10.1016/j.seppur.2024.126673

Design and evolution of a synthetic small protein scaffold based on the WW domain

Autores: Dias AMGC, Teixeira GDG, Barbosa AJM, Goncalves J, Iranzo O, Roque ACA
Publicado en: Protein Science, 2025, ISSN 1018-4783
Editor: Protein Science
DOI: 10.1002/pro.70164

High‐Yield Production and Cost‐Effective Purification of an Alkali‐Resistant Hexameric Protein A Variant for Antibody Affinity Chromatography

Autores: Carolina Mota Natal, Claudia Lacombe, Alexander Zollner, Catarina Domingos, José Mendes, André Nascimento, Ana Margarida Gonçalves Carvalho Dias, Arménio Jorge Moura Barbosa, Cristina Peixoto, Birgit Wiltschi, Alois Jungbauer, Ana Cecília Afonso Roque
Publicado en: Biotechnology Journal, Edición 21, 2026, ISSN 1860-6768
Editor: Wiley - VCH Verlag GmbH & CO. KGaA
DOI: 10.1002/biot.70247

Enhancing NA immunogenicity through novel VLP designs.

Autores: Leticia Guzman Ruiz, Alexander M. Zollner, Irene Hoxie, Jan Küchler, Christina Hausjell, Tomas Mesurado, Florian Krammer, Alois Jungbauer, Patricia Pereira Aguilar, Miriam Klausberger, Reingard Grabherr
Publicado en: Vaccine, Edición 42, 2024, Página(s) 126270, ISSN 0264-410X
Editor: Elsevier BV
DOI: 10.1016/j.vaccine.2024.126270

Rational design of a small peptidomimetic ligand to capture a viral spike protein

Autores: Carlos Filipe Santos Costa, Iana Lychko, Carolina Mota Natal, Arménio Jorge Moura Barbosa, Ana Margarida Gonçalves Carvalho Dias, Ana Cecília Afonso Roque
Publicado en: Separation and Purification Technology, Edición 366, 2025, Página(s) 132778, ISSN 1383-5866
Editor: Pergamon Press Ltd.
DOI: 10.1016/j.seppur.2025.132778

One‐Pot Synthesis of a Small Synthetic Ligand for Antibody Purification

Autores: Carolina Mota Natal, Catarina Domingos, André Nascimento, José Mendes, Afonso B. Ruiz, Ana Margarida Gonçalves Carvalho Dias, Luisa M. Ferreira, Cristina Peixoto, Ana Cecília Afonso Roque
Publicado en: Biotechnology and Bioengineering, 2026, ISSN 0006-3592
Editor: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.1002/bit.70226

Directed Functionalization of Recombinant Spider Silk Nonwoven Membranes with Antibodies Using Non‐Canonical Amino Acids

Autores: Claudia Lacombe, Charlotte Leonhardt, Martin Humenik, Birgit Wiltschi, Thomas Scheibel
Publicado en: Advanced Materials, Edición 38, 2026, ISSN 0935-9648
Editor: United Nations Industrial Developement Organization
DOI: 10.1002/adma.202519707

Suitable electrospinning approaches for recombinant spider silk proteins

Autores: Christoph Sommer, Thomas Scheibel
Publicado en: Open Ceramics, Edición 15, 2023, Página(s) 100420, ISSN 2666-5395
Editor: Elsevier
DOI: 10.1016/j.oceram.2023.100420

Native, engineered and de novo designed ligands targeting the SARS-CoV-2 spike protein

Autores: Costa, Carlos Filipe Santos; Barbosa, Arménio J. M.; Dias, Ana Margarida Gonçalves Carvalho; Roque, Ana Cecília A.
Publicado en: Biotechnology Advances, Edición 59, 2022, Página(s) 107986, ISSN 0734-9750
Editor: Elsevier BV
DOI: 10.1016/j.biotechadv.2022.107986

Cephalopod proteins for bioinspired and sustainable biomaterials design

Autores: Iana Lychko, Inês Padrão, Afonso Vicente Eva, Catarina Alexandra Oliveira Domingos, Henrique Miguel Aljustrel da Costa, Ana Margarida Gonçalves Carvalho Dias, Ana Cecília Afonso Roque
Publicado en: Materials Today Bio, Edición 31, 2025, Página(s) 101644, ISSN 2590-0064
Editor: Elsevier BV
DOI: 10.1016/j.mtbio.2025.101644

Solvent modulation in peptide sub-microfibers obtained by solution blow spinning

Autores: Ana Margarida Gonçalves Carvalho Dias, Cícero Cena, Viviane Lutz-Bueno, Raffaele Mezzenga, Ana Marques, Isabel Ferreira, Ana Cecília Afonso Roque
Publicado en: Frontiers in Chemistry, Edición 10, 2022, ISSN 2296-2646
Editor: Frontiers Media SA
DOI: 10.3389/fchem.2022.1054347

Thesis and dissertations (5)

To be defined

Autores: Alexander Zollner
Publicado en: 2025
Editor: BOKU

Affinity reagents towardsSars-cov-2

Autores: Carlos Costa
Publicado en: 2025
Editor: Universidade NOVA de Lisboa

Novel Affinity-Based Technologies for Targetting Distinct Biological Systems

Autores: Eduardo Almeida
Publicado en: 2024
Editor: iBET

Exploring alternative culture media for protein recombinant expression in bacterial hosts

Autores: Renata Costa
Publicado en: 2025
Editor: Universidade NOVA de Lisboa

Sustainable fibers for bioseparation: innovation, market transfer and a collaborative model

Autores: José Pedro Fradique Lourenço Dias Dinis
Publicado en: 2022
Editor: Universidade NOVA de Lisboa

Book chapters (1)

Technology Market Transfer in Biotechnology: The Case Study of PURE Project

Autores: José Dinis, Fernanda Llussá, Paula Urze and Ana Cecília Roque
Publicado en: DECIDE SMARTER IN BUSINESS AND LIFE: TRANSFORMING DECISION MAKING WITH VCW, 2025, ISBN 978-989-53346-2-9
Editor: Nova School of Business and Economics, Universidade NOVA de Lisboa. Portugal