Precisely Patterned Nanofibers for High Performance Bioseparations

Informations projet

PURE

N° de convention de subvention: 899732

DOI

10.3030/899732

Projet clôturé

Date de signature de la CE 26 Mai 2020

Date de début 1 Octobre 2020

Date de fin 31 Mars 2025

Financé au titre de

• EXCELLENT SCIENCE - Future and Emerging Technologies (FET)

Coût total

€ 2 999 537,50

Contribution de l’UE

€ 2 999 537,50

Coordonné par

UNIVERSIDADE NOVA DE LISBOA
Portugal

CORDIS fournit des liens vers les livrables publics et les publications des projets HORIZON.

Les liens vers les livrables et les publications des projets du 7e PC, ainsi que les liens vers certains types de résultats spécifiques tels que les jeux de données et les logiciels, sont récupérés dynamiquement sur OpenAIRE .

Livrables

Other (4)

Bioproduction of non-canonical amino acids

At least 500 mg of reactive ncAA AhA will be produced by biotechnological means

Spidroin models

Unmodified, labeled and ligand modified spidroin (in solution and in fibrils) will be analyzed by SAXS (BOKU) and then modeled by NOVA.At least 10 multiscale models of modified spidroin are expected.

Bioproduction of unmodified spidroins

At least 500 mg of unmodified spidroins will be biologically produced

Production of activated affinity ligands

At least 250 mg of each activated affinity ligand will be attained The affinity ligands selective for factor VIII Mabs VLPs and protein A will be produced and modified with DBCO for further modification of labeled spidroin

Open Research Data Pilot (1)

Data Management Plan

Data Management PlanA detailed Data Management Plan will be created at the beginning of the project to ensure that all data is findable accessible interoperable and reusable FAIR The plan will be harmonized with a knowledge protection plan IPR plan

Websites, patent fillings, videos etc. (2)

Future exploitation

Collaborative framework model & business plan with list of stakeholders for future exploitation

PURE webportal operational

Documents, reports (1)

Dissemination and Exploitation Plan

Dissemination and Exploitation Plan at the end of the first Reporting Period RP1 M12

Publications

Peer reviewed articles (11)

Heparin-affinity chromatography is a generic purification platform for chimeric gag VLPs displaying different viral surface antigens

Auteurs: Alexander M. Zollner, Leticia Guzman Ruiz, Viktoria Mayer, Stefanie Stohl, Leo A. Jakob, Nico Lingg, Miriam Klausberger, Alois Jungbauer, Patricia Pereira Aguilar
Publié dans: Separation and Purification Technology, Numéro 340, 2024, Page(s) 126673, ISSN 1383-5866
Éditeur: Pergamon Press Ltd.
DOI: 10.1016/j.seppur.2024.126673

Design and evolution of a synthetic small protein scaffold based on the WW domain

Auteurs: Dias AMGC, Teixeira GDG, Barbosa AJM, Goncalves J, Iranzo O, Roque ACA
Publié dans: Protein Science, 2025, ISSN 1018-4783
Éditeur: Protein Science
DOI: 10.1002/pro.70164

High‐Yield Production and Cost‐Effective Purification of an Alkali‐Resistant Hexameric Protein A Variant for Antibody Affinity Chromatography

Auteurs: Carolina Mota Natal, Claudia Lacombe, Alexander Zollner, Catarina Domingos, José Mendes, André Nascimento, Ana Margarida Gonçalves Carvalho Dias, Arménio Jorge Moura Barbosa, Cristina Peixoto, Birgit Wiltschi, Alois Jungbauer, Ana Cecília Afonso Roque
Publié dans: Biotechnology Journal, Numéro 21, 2026, ISSN 1860-6768
Éditeur: Wiley - VCH Verlag GmbH & CO. KGaA
DOI: 10.1002/biot.70247

Enhancing NA immunogenicity through novel VLP designs.

Auteurs: Leticia Guzman Ruiz, Alexander M. Zollner, Irene Hoxie, Jan Küchler, Christina Hausjell, Tomas Mesurado, Florian Krammer, Alois Jungbauer, Patricia Pereira Aguilar, Miriam Klausberger, Reingard Grabherr
Publié dans: Vaccine, Numéro 42, 2024, Page(s) 126270, ISSN 0264-410X
Éditeur: Elsevier BV
DOI: 10.1016/j.vaccine.2024.126270

Rational design of a small peptidomimetic ligand to capture a viral spike protein

Auteurs: Carlos Filipe Santos Costa, Iana Lychko, Carolina Mota Natal, Arménio Jorge Moura Barbosa, Ana Margarida Gonçalves Carvalho Dias, Ana Cecília Afonso Roque
Publié dans: Separation and Purification Technology, Numéro 366, 2025, Page(s) 132778, ISSN 1383-5866
Éditeur: Pergamon Press Ltd.
DOI: 10.1016/j.seppur.2025.132778

One‐Pot Synthesis of a Small Synthetic Ligand for Antibody Purification

Auteurs: Carolina Mota Natal, Catarina Domingos, André Nascimento, José Mendes, Afonso B. Ruiz, Ana Margarida Gonçalves Carvalho Dias, Luisa M. Ferreira, Cristina Peixoto, Ana Cecília Afonso Roque
Publié dans: Biotechnology and Bioengineering, 2026, ISSN 0006-3592
Éditeur: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.1002/bit.70226

Directed Functionalization of Recombinant Spider Silk Nonwoven Membranes with Antibodies Using Non‐Canonical Amino Acids

Auteurs: Claudia Lacombe, Charlotte Leonhardt, Martin Humenik, Birgit Wiltschi, Thomas Scheibel
Publié dans: Advanced Materials, Numéro 38, 2026, ISSN 0935-9648
Éditeur: United Nations Industrial Developement Organization
DOI: 10.1002/adma.202519707

Suitable electrospinning approaches for recombinant spider silk proteins

Auteurs: Christoph Sommer, Thomas Scheibel
Publié dans: Open Ceramics, Numéro 15, 2023, Page(s) 100420, ISSN 2666-5395
Éditeur: Elsevier
DOI: 10.1016/j.oceram.2023.100420

Native, engineered and de novo designed ligands targeting the SARS-CoV-2 spike protein

Auteurs: Costa, Carlos Filipe Santos; Barbosa, Arménio J. M.; Dias, Ana Margarida Gonçalves Carvalho; Roque, Ana Cecília A.
Publié dans: Biotechnology Advances, Numéro 59, 2022, Page(s) 107986, ISSN 0734-9750
Éditeur: Elsevier BV
DOI: 10.1016/j.biotechadv.2022.107986

Cephalopod proteins for bioinspired and sustainable biomaterials design

Auteurs: Iana Lychko, Inês Padrão, Afonso Vicente Eva, Catarina Alexandra Oliveira Domingos, Henrique Miguel Aljustrel da Costa, Ana Margarida Gonçalves Carvalho Dias, Ana Cecília Afonso Roque
Publié dans: Materials Today Bio, Numéro 31, 2025, Page(s) 101644, ISSN 2590-0064
Éditeur: Elsevier BV
DOI: 10.1016/j.mtbio.2025.101644

Solvent modulation in peptide sub-microfibers obtained by solution blow spinning

Auteurs: Ana Margarida Gonçalves Carvalho Dias, Cícero Cena, Viviane Lutz-Bueno, Raffaele Mezzenga, Ana Marques, Isabel Ferreira, Ana Cecília Afonso Roque
Publié dans: Frontiers in Chemistry, Numéro 10, 2022, ISSN 2296-2646
Éditeur: Frontiers Media SA
DOI: 10.3389/fchem.2022.1054347

Thesis and dissertations (5)

To be defined

Auteurs: Alexander Zollner
Publié dans: 2025
Éditeur: BOKU

Affinity reagents towardsSars-cov-2

Auteurs: Carlos Costa
Publié dans: 2025
Éditeur: Universidade NOVA de Lisboa

Novel Affinity-Based Technologies for Targetting Distinct Biological Systems

Auteurs: Eduardo Almeida
Publié dans: 2024
Éditeur: iBET

Exploring alternative culture media for protein recombinant expression in bacterial hosts

Auteurs: Renata Costa
Publié dans: 2025
Éditeur: Universidade NOVA de Lisboa

Sustainable fibers for bioseparation: innovation, market transfer and a collaborative model

Auteurs: José Pedro Fradique Lourenço Dias Dinis
Publié dans: 2022
Éditeur: Universidade NOVA de Lisboa

Book chapters (1)

Technology Market Transfer in Biotechnology: The Case Study of PURE Project

Auteurs: José Dinis, Fernanda Llussá, Paula Urze and Ana Cecília Roque
Publié dans: DECIDE SMARTER IN BUSINESS AND LIFE: TRANSFORMING DECISION MAKING WITH VCW, 2025, ISBN 978-989-53346-2-9
Éditeur: Nova School of Business and Economics, Universidade NOVA de Lisboa. Portugal