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Revealing the immune tumor microenvironment (iTME) in melanoma by multiplexed imaging

Project description

Unravelling the role of melanoma microenvironment in immunotherapy response

Tumours are spatially organised assemblies of distinct cell types with phenotypes defined by the coexpression of multiple proteins. The immune tumour microenvironment (iTME) plays a key role in immune and stromal cell interactions, defining progression and response to treatment. A recently introduced multiplexed ion beam imaging platform enables the simultaneous imaging of 40 proteins within intact tissue sections at subcellular resolution. The EU-funded ImageMelanoma project proposes to use this platform to delve into the iTME in dozens of samples, defining its function in response to different immunotherapies. The study will profile melanoma tumours to elucidate mechanisms that drive iTME organisation, develop new experimental tools for tracing and barcoding thousands of tumour cells and employ algorithms based on machine learning for data analysis.

Objective

Immunotherapies targeting immune regulators are revolutionizing cancer treatment, most prominently in melanoma, but only for a subset of patients. While it is known that the immune tumor microenvironment (iTME) plays a vital role in this process, there is limited understanding on how distinct tumor, immune and stroma cells interact as a system to collectively define progression and response to treatment, and there is no biomarker to predict patient response. Tumors are spatially organized ecosystems that are comprised of distinct cell types, each of which can assume a variety of phenotypes defined by coexpression of multiple proteins. To underscore this complexity, and move beyond single cells to multicellular interactions, it is essential to interrogate cellular expression patterns within their native context in the tissue.

We have recently pioneered MIBI-TOF (Multiplexed Ion Beam Imaging by Time of Flight), a novel platform that enables simultaneous imaging of forty proteins within intact tissue sections at subcellular resolution. We propose to (1) Use MIBI-TOF to chart the iTME in dozens of clinical samples from melanoma patients and delineate its function in response to different immunotherapies. (2) Profile murine melanoma tumors to elucidate genetic and temporal mechanisms that drive iTME organization in vivo. (3) Develop new experimental tools for tracing and barcoding thousands of cells to decouple the effects of tumor genetics and the immune microenvironment on tumor organization and clonal dynamics. (4) Develop machine-learning-based algorithms to analyze this novel data and facilitate accessibility of the scientific community to high-dimensional imaging to study human malignancies.

This proposal applies state-of-the-art imaging technology and computation to unravel design principles of the iTME in melanoma, with a grand goal to reveal basic principles in tumor immunology and improve treatment and diagnostics.

Host institution

WEIZMANN INSTITUTE OF SCIENCE
Net EU contribution
€ 1 613 750,00
Address
HERZL STREET 234
7610001 Rehovot
Israel

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Activity type
Higher or Secondary Education Establishments
Links
Total cost
€ 1 613 750,00

Beneficiaries (1)