Descrizione del progetto DEENESFRITPL Fare luce sul fenomeno delle sottopopolazioni In tutti i tipi di cellule, popolazioni geneticamente identiche possono formare sottopopolazioni distinte dal punto di vista metabolico. Tuttavia, l’emergere di sottopopolazioni è un aspetto largamente trascurato nell’ambito della biotecnologia. Il progetto DEUSBIO, finanziato dall’UE, indagherà l’emergere delle sottopopolazioni nel lievito: usando nuovi strumenti di biologia sintetica, tenterà infatti di identificare le loro implicazioni nel metabolismo e nella bioproduzione, e produrrà un quadro innovativo per massimizzare la biosintesi di molecole ad alto valore. I risultati del progetto avranno implicazioni non solo per la bioproduzione, ma anche per la biomedicina, l’eterogeneità del cancro e la complessità del microbioma. Mostra l’obiettivo del progetto Nascondi l’obiettivo del progetto Obiettivo Microbial bioproduction, despite being considered a paradigmatic sustainable alternative to petroleum-based chemistry, is often limited by low yields and productivities, which prevents commercialisation. It is generally known for all types of cells that genetically identical populations can form metabolically distinct subpopulations. This diversity strongly impairs bioproduction as the presence of low-producer or slow-grower cells reduces overall yields. However, the universal phenomenon of subpopulations emergence has been largely overlooked, especially in biotechnology, due to technical difficulties. Now, thanks to recent developments in single cell technologies, in molecular understanding of microbial communities and in synthetic biology tools, we can begin to address this widespread and impactful biological feature. I propose to explore the emergence of subpopulations in yeast and understand their implications in metabolism and bioproduction using and developing cutting edge synthetic biology tools. I aim to use that knowledge to develop novel engineered strains that lack the presence of undesired subpopulations and then use such homogeneous populations for bioproduction. The homogenised production will be investigated in both, monocultures and microbial communities. In DEUSBIO, I will set up an innovative framework to maximise the biosynthesis of high value molecules, with high potential to overcome current limitations.This project will shed light on the phenomenon of subpopulations, whose relevance goes beyond bioproduction, as for example, it has been associated with the origin of multicellularity. Increasing our knowledge about this matter will also have implications in biomedicine, as cell subpopulations are extremely important in the appearance of antimicrobial resistant, in cancer heterogeneity, and in microbiome complexity. Campo scientifico natural sciencesbiological sciencessynthetic biologysocial scienceseconomics and businesseconomicsproduction economicsproductivitymedical and health sciencesclinical medicineoncologynatural sciencesbiological sciencesmicrobiologymedical and health sciencesmedical biotechnologycells technologies Parole chiave synthetic biology metabolic engineering microbial bioproduction microbial biotechnology microbial cell factories Programma(i) H2020-EU.1.1. - EXCELLENT SCIENCE - European Research Council (ERC) Main Programme Argomento(i) ERC-2020-STG - ERC STARTING GRANTS Invito a presentare proposte ERC-2020-STG Vedi altri progetti per questo bando Meccanismo di finanziamento ERC-STG - Starting Grant Istituzione ospitante IMPERIAL COLLEGE OF SCIENCE TECHNOLOGY AND MEDICINE Contribution nette de l'UE € 1 499 998,00 Indirizzo SOUTH KENSINGTON CAMPUS EXHIBITION ROAD SW7 2AZ LONDON Regno Unito Mostra sulla mappa Regione London Inner London — West Westminster Tipo di attività Higher or Secondary Education Establishments Collegamenti Contatta l’organizzazione Opens in new window Sito web Opens in new window Partecipazione a programmi di R&I dell'UE Opens in new window Rete di collaborazione HORIZON Opens in new window Costo totale € 1 499 998,00 Beneficiari (1) Classifica in ordine alfabetico Classifica per Contributo netto dell'UE Espandi tutto Riduci tutto IMPERIAL COLLEGE OF SCIENCE TECHNOLOGY AND MEDICINE Regno Unito Contribution nette de l'UE € 1 499 998,00 Indirizzo SOUTH KENSINGTON CAMPUS EXHIBITION ROAD SW7 2AZ LONDON Mostra sulla mappa Regione London Inner London — West Westminster Tipo di attività Higher or Secondary Education Establishments Collegamenti Contatta l’organizzazione Opens in new window Sito web Opens in new window Partecipazione a programmi di R&I dell'UE Opens in new window Rete di collaborazione HORIZON Opens in new window Costo totale € 1 499 998,00
