SPAtially-Controlled lIgand arraNGement by origami-based nanoprinters

Informations projet

SPACING

N° de convention de subvention: 950421

DOI

10.3030/950421

Date de signature de la CE 3 Novembre 2020

Date de début 1 Avril 2021

Date de fin 30 Septembre 2027

Financé au titre de

• EXCELLENT SCIENCE - European Research Council (ERC)

Coût total

€ 1 498 866,00

Contribution de l’UE

€ 1 498 866,00

Coordonné par

UNIVERSIDAD DE SANTIAGO DE COMPOSTELA
Spain

Periodic Reporting for period 3 - SPACING (SPAtially-Controlled lIgand arraNGement by origami-based nanoprinters)

Période du rapport: 2024-04-01 au 2025-09-30

Résumé du contexte et des objectifs généraux du projet

SPACING project aims to develop a new technology for surface functionalization of colloids with control over the number and the spatial disposition of the ligands. Currently, the we-chemistry methods are not allowing to have this control in the colloids modification. Thus, we propose the production of nanoprinters built with DNA (DNA-origamis) that will serve to precisely modified colloids (i.e. gold nanoparticles, as a model). This technology is expected to be used for any colloid as the nanoprinters can be fully customized.

Upon the development of this technology, colloids´libraries will be developed to study their biological behavior in different models. The systematic study of what is the role of the spatial distribution of bioactive molecules in colloids will allow us to understand better processes involving receptor-recognitions, and some intracellular processes such as the endosomal scape or some recycling process.

We expect that this technology will allow for the development of rational rules for the development of more efficient nanomedicines.
Currently, we have developed some models of nanoprinters capable of transferring to gold nanoparticles a controlled number of DNA strands with a defined spatial configuration allowing us to demonstrate the principle.

Travail effectué depuis le début du projet jusqu’à la fin de la période considérée dans le rapport et principaux résultats atteints jusqu’à présent

SPACING project aims to develop a new technology for surface functionalization of colloids with control over the number and the spatial disposition of the ligands. Currently, the we-chemistry methods are not allowing to have this control in the colloids modification. Thus, we propose the production of nanoprinters built with DNA (DNA-origamis) that will serve to precisely modified colloids (i.e. gold nanoparticles, as a model). This technology is expected to be used for any colloid as the nanoprinters can be fully customized.

Upon the development of this technology, colloids´libraries will be developed to study their biological behavior in different models. The systematic study of what is the role of the spatial distribution of bioactive molecules in colloids will allow us to understand better processes involving receptor-recognitions, and some intracellular processes such as the endosomal scape or some recycling process.

We expect that this technology will allow for the development of rational rules for the development of more efficient nanomedicines.
Currently, we have developed some models of nanoprinters capable of transferring to gold nanoparticles a controlled number of DNA strands with a defined spatial configuration allowing us to demonstrate the principle

Progrès au-delà de l’état des connaissances et impact potentiel prévu (y compris l’impact socio-économique et les conséquences sociétales plus larges du projet jusqu’à présent)

We expect that this technology will move forward the existing capabilities for tuning colloid´s surfaces. We aim to develop a user-friendly, robust technology easily used in many laboratories.
The technology will offer the possibility of produce colloid with extremely define composition that could be used in bioapplications, but also for electronics, to produce metamaterials, etc.