SPAtially-Controlled lIgand arraNGement by origami-based nanoprinters

Projektinformationen

SPACING

ID Finanzhilfevereinbarung: 950421

DOI

10.3030/950421

EK-Unterschriftsdatum 3 November 2020

Startdatum 1 April 2021

Enddatum 30 September 2027

Finanziert unter

• EXCELLENT SCIENCE - European Research Council (ERC)

Gesamtkosten

€ 1 498 866,00

EU-Beitrag

€ 1 498 866,00

Koordiniert durch

UNIVERSIDAD DE SANTIAGO DE COMPOSTELA
Spain

Periodic Reporting for period 3 - SPACING (SPAtially-Controlled lIgand arraNGement by origami-based nanoprinters)

Berichtszeitraum: 2024-04-01 bis 2025-09-30

Zusammenfassung vom Kontext und den Gesamtzielen des Projekts

SPACING project aims to develop a new technology for surface functionalization of colloids with control over the number and the spatial disposition of the ligands. Currently, the we-chemistry methods are not allowing to have this control in the colloids modification. Thus, we propose the production of nanoprinters built with DNA (DNA-origamis) that will serve to precisely modified colloids (i.e. gold nanoparticles, as a model). This technology is expected to be used for any colloid as the nanoprinters can be fully customized.

Upon the development of this technology, colloids´libraries will be developed to study their biological behavior in different models. The systematic study of what is the role of the spatial distribution of bioactive molecules in colloids will allow us to understand better processes involving receptor-recognitions, and some intracellular processes such as the endosomal scape or some recycling process.

We expect that this technology will allow for the development of rational rules for the development of more efficient nanomedicines.
Currently, we have developed some models of nanoprinters capable of transferring to gold nanoparticles a controlled number of DNA strands with a defined spatial configuration allowing us to demonstrate the principle.

Arbeit, die ab Beginn des Projekts bis zum Ende des durch den Bericht erfassten Berichtszeitraums geleistet wurde, und die wichtigsten bis dahin erzielten Ergebnisse

SPACING project aims to develop a new technology for surface functionalization of colloids with control over the number and the spatial disposition of the ligands. Currently, the we-chemistry methods are not allowing to have this control in the colloids modification. Thus, we propose the production of nanoprinters built with DNA (DNA-origamis) that will serve to precisely modified colloids (i.e. gold nanoparticles, as a model). This technology is expected to be used for any colloid as the nanoprinters can be fully customized.

Upon the development of this technology, colloids´libraries will be developed to study their biological behavior in different models. The systematic study of what is the role of the spatial distribution of bioactive molecules in colloids will allow us to understand better processes involving receptor-recognitions, and some intracellular processes such as the endosomal scape or some recycling process.

We expect that this technology will allow for the development of rational rules for the development of more efficient nanomedicines.
Currently, we have developed some models of nanoprinters capable of transferring to gold nanoparticles a controlled number of DNA strands with a defined spatial configuration allowing us to demonstrate the principle

Fortschritte, die über den aktuellen Stand der Technik hinausgehen und voraussichtliche potenzielle Auswirkungen (einschließlich der bis dato erzielten sozioökonomischen Auswirkungen und weiter gefassten gesellschaftlichen Auswirkungen des Projekts)

We expect that this technology will move forward the existing capabilities for tuning colloid´s surfaces. We aim to develop a user-friendly, robust technology easily used in many laboratories.
The technology will offer the possibility of produce colloid with extremely define composition that could be used in bioapplications, but also for electronics, to produce metamaterials, etc.