SPAtially-Controlled lIgand arraNGement by origami-based nanoprinters

Informazioni relative al progetto

SPACING

ID dell’accordo di sovvenzione: 950421

DOI

10.3030/950421

Data della firma CE 3 Novembre 2020

Data di avvio 1 Aprile 2021

Data di completamento 30 Settembre 2027

Finanziato da

• EXCELLENT SCIENCE - European Research Council (ERC)

Costo totale

€ 1 498 866,00

Contributo UE

€ 1 498 866,00

Coordinato da

UNIVERSIDAD DE SANTIAGO DE COMPOSTELA
Spain

Periodic Reporting for period 3 - SPACING (SPAtially-Controlled lIgand arraNGement by origami-based nanoprinters)

Periodo di rendicontazione: 2024-04-01 al 2025-09-30

Sintesi del contesto e degli obiettivi generali del progetto

SPACING project aims to develop a new technology for surface functionalization of colloids with control over the number and the spatial disposition of the ligands. Currently, the we-chemistry methods are not allowing to have this control in the colloids modification. Thus, we propose the production of nanoprinters built with DNA (DNA-origamis) that will serve to precisely modified colloids (i.e. gold nanoparticles, as a model). This technology is expected to be used for any colloid as the nanoprinters can be fully customized.

Upon the development of this technology, colloids´libraries will be developed to study their biological behavior in different models. The systematic study of what is the role of the spatial distribution of bioactive molecules in colloids will allow us to understand better processes involving receptor-recognitions, and some intracellular processes such as the endosomal scape or some recycling process.

We expect that this technology will allow for the development of rational rules for the development of more efficient nanomedicines.
Currently, we have developed some models of nanoprinters capable of transferring to gold nanoparticles a controlled number of DNA strands with a defined spatial configuration allowing us to demonstrate the principle.

Lavoro eseguito dall’inizio del progetto fino alla fine del periodo coperto dalla relazione e principali risultati finora ottenuti

SPACING project aims to develop a new technology for surface functionalization of colloids with control over the number and the spatial disposition of the ligands. Currently, the we-chemistry methods are not allowing to have this control in the colloids modification. Thus, we propose the production of nanoprinters built with DNA (DNA-origamis) that will serve to precisely modified colloids (i.e. gold nanoparticles, as a model). This technology is expected to be used for any colloid as the nanoprinters can be fully customized.

Upon the development of this technology, colloids´libraries will be developed to study their biological behavior in different models. The systematic study of what is the role of the spatial distribution of bioactive molecules in colloids will allow us to understand better processes involving receptor-recognitions, and some intracellular processes such as the endosomal scape or some recycling process.

We expect that this technology will allow for the development of rational rules for the development of more efficient nanomedicines.
Currently, we have developed some models of nanoprinters capable of transferring to gold nanoparticles a controlled number of DNA strands with a defined spatial configuration allowing us to demonstrate the principle

Progressi oltre lo stato dell’arte e potenziale impatto previsto (incluso l’impatto socioeconomico e le implicazioni sociali più ampie del progetto fino ad ora)

We expect that this technology will move forward the existing capabilities for tuning colloid´s surfaces. We aim to develop a user-friendly, robust technology easily used in many laboratories.
The technology will offer the possibility of produce colloid with extremely define composition that could be used in bioapplications, but also for electronics, to produce metamaterials, etc.