In-situ spatiotemporal imaging of membrane hydration, electrostatics, tension and curvature to understand cell response to osmotic shocks and cell migration.

Project Information

R2-TENSION

Grant agreement ID: 951324

DOI 10.3030/951324

EC signature date 14 January 2021

Start date 1 November 2021

End date 31 October 2027

Funded under

• EXCELLENT SCIENCE - European Research Council (ERC)

Total cost € 4 947 465,00

EU contribution € 4 947 465,00

Coordinated by

ECOLE POLYTECHNIQUE FEDERALE DE LAUSANNE
Switzerland

Publications

Peer reviewed articles (11)

Ion-Induced Transient Potential Fluctuations Facilitate Pore Formation and Cation Transport through Lipid Membranes.

Author(s): David Roesel; Maksim Eremchev; Chetan S. Poojari; Jochen S. Hub; Sylvie Roke
Published in: J. Am. Chem. Soc., Issue 1, 2022, ISSN 0002-7863
Publisher: American Chemical Society
DOI: 10.48550/arxiv.2207.10963

HydroFlipper membrane tension probes: imaging membrane hydration and mechanical compression simultaneously in living cells

Author(s): José García-Calvo; Javier López-Andarias; Jimmy Maillard; Vincent Mercier; Chloé Roffay; Aurélien Roux; Alexandre Fürstenberg; Naomi Sakai; Stefan Matile
Published in: Chemical science, Issue 1, 2022, ISSN 2041-6520
Publisher: Royal Society of Chemistry
DOI: 10.1039/d1sc05208j

Passive coupling of membrane tension and cell volume during active response of cells to osmosis

Author(s): Chloé Roffay, Guillaume Molinard, Kyoohyun Kim, Marta Urbanska, Virginia Andrade, Victoria Barbarasa, Paulina Nowak, Vincent Mercier, José García-Calvo, Stefan Matile, Robbie Loewith, Arnaud Echard, Jochen Guck, Martin Lenz, Aurélien Roux
Published in: Proceedings of the National Academy of Sciences, 2021, ISSN 0027-8424
Publisher: National Academy of Sciences
DOI: 10.1073/pnas.2103228118

Integer topological defects organize stresses driving tissue morphogenesis

Author(s): Pau Guillamat, Carles Blanch-Mercader, Guillaume Pernollet, Karsten Kruse, Aurélien Roux
Published in: Nature Materials, 2022, ISSN 1476-1122
Publisher: Nature Publishing Group
DOI: 10.1038/s41563-022-01194-5

Passive transport of Ca2+ ions through lipid bilayers imaged by wide-field second harmonic microscopy

Author(s): M. Eremchev; D. Roesel; C. S. Poojari; A. Roux; J. S. Hub; S. Roke
Published in: Biophys. J., 2023, ISSN 0006-3495
Publisher: Biophysical Society
DOI: 10.1016/j.bpj.2023.01.018

Epithelial cells adapt to curvature induction via transient active osmotic swelling

Author(s): Caterina Tomba, Valeriy Luchnikov, Luca Barberi, Carles Blanch-Mercader, Aurélien Roux
Published in: Developmental Cell, 2022, ISSN 1534-5807
Publisher: Cell Press
DOI: 10.1016/j.devcel.2022.04.017

Interfacial Inversion, Interference, and IR Absorption in Vibrational Sum Frequency Scattering Experiments

Author(s): S. Pullanchery, L. Zhang, S. Kulik, and S. Roke
Published in: Journal Of Physical Chemistry B, 2023, ISSN 1520-6106
Publisher: American Chemical Society
DOI: 10.1021/acs.jpcb.3c02727

Water as a contrast agent to quantify surface chemistry and physics using second harmonic scattering and imaging: A perspective

Author(s): D. Roesel, M. Eremchev, T. Schönfeldová, S. Lee, S. Roke
Published in: Appl. Phys. Lett., 2022, ISSN 0003-6951
Publisher: American Institute of Physics
DOI: 10.1063/5.0085807

Liposomes and Lipid Droplets Display a Reversal of Charge-Induced Hydration Asymmetry

Author(s): Saranya Pullanchery, Nathan Dupertuis, Tereza Roesel, Sylvie Roke
Published in: Nano Letters, 2023, ISSN 1530-6984
Publisher: American Chemical Society
DOI: 10.1021/acs.nanolett.3c02653

High throughput wide field second harmonic imaging of giant unilamellar vesicles

Author(s): M. Eremchev, D. Roesel, P.-M. Dansette, A. Michailovas, S. Roke
Published in: Biointerphases, 2023, ISSN 1559-4106
Publisher: American Vacuum Society
DOI: 10.1116/6.0002640

Pressure and curvature control of the cell cycle in epithelia growing under spherical confinement

Author(s): Morphogenesis requires spatiotemporal regulation of proliferation, both by biochemical and mechanical cues. In epithelia, this regulation is called contact inhibition of proliferation, but disentangling biochemical from mechanical cues remains challenging. Here, we show that epithelia growing under confinement accumulate pressure that inhibits proliferation above a threshold value. During growth,
Published in: Cell Reports, 2022, ISSN 2211-1247
Publisher: Cell Press
DOI: 10.1016/j.celrep.2022.111227