In-situ spatiotemporal imaging of membrane hydration, electrostatics, tension and curvature to understand cell response to osmotic shocks and cell migration.

Informazioni relative al progetto

R2-TENSION

ID dell’accordo di sovvenzione: 951324

DOI

10.3030/951324

Data della firma CE 14 Gennaio 2021

Data di avvio 1 Novembre 2021

Data di completamento 31 Ottobre 2027

Finanziato da

• EXCELLENT SCIENCE - European Research Council (ERC)

Costo totale

€ 4 947 465,00

Contributo UE

€ 4 947 465,00

Coordinato da

ECOLE POLYTECHNIQUE FEDERALE DE LAUSANNE
Switzerland

CORDIS fornisce collegamenti ai risultati finali pubblici e alle pubblicazioni dei progetti ORIZZONTE.

I link ai risultati e alle pubblicazioni dei progetti del 7° PQ, così come i link ad alcuni tipi di risultati specifici come dataset e software, sono recuperati dinamicamente da .OpenAIRE .

Pubblicazioni

Peer reviewed articles (14)

Ion-Induced Transient Potential Fluctuations Facilitate Pore Formation and Cation Transport through Lipid Membranes.

Autori: David Roesel; Maksim Eremchev; Chetan S. Poojari; Jochen S. Hub; Sylvie Roke
Pubblicato in: J. Am. Chem. Soc., Numero 1, 2022, ISSN 0002-7863
Editore: American Chemical Society
DOI: 10.48550/arxiv.2207.10963

HydroFlipper membrane tension probes: imaging membrane hydration and mechanical compression simultaneously in living cells

Autori: José García-Calvo; Javier López-Andarias; Jimmy Maillard; Vincent Mercier; Chloé Roffay; Aurélien Roux; Alexandre Fürstenberg; Naomi Sakai; Stefan Matile
Pubblicato in: Chemical science, Numero 1, 2022, ISSN 2041-6520
Editore: Royal Society of Chemistry
DOI: 10.1039/d1sc05208j

Passive coupling of membrane tension and cell volume during active response of cells to osmosis

Autori: Chloé Roffay, Guillaume Molinard, Kyoohyun Kim, Marta Urbanska, Virginia Andrade, Victoria Barbarasa, Paulina Nowak, Vincent Mercier, José García-Calvo, Stefan Matile, Robbie Loewith, Arnaud Echard, Jochen Guck, Martin Lenz, Aurélien Roux
Pubblicato in: Proceedings of the National Academy of Sciences, 2021, ISSN 0027-8424
Editore: National Academy of Sciences
DOI: 10.1073/pnas.2103228118

Integer topological defects organize stresses driving tissue morphogenesis

Autori: Pau Guillamat, Carles Blanch-Mercader, Guillaume Pernollet, Karsten Kruse, Aurélien Roux
Pubblicato in: Nature Materials, 2022, ISSN 1476-1122
Editore: Nature Publishing Group
DOI: 10.1038/s41563-022-01194-5

Passive transport of Ca2+ ions through lipid bilayers imaged by wide-field second harmonic microscopy

Autori: M. Eremchev; D. Roesel; C. S. Poojari; A. Roux; J. S. Hub; S. Roke
Pubblicato in: Biophys. J., 2023, ISSN 0006-3495
Editore: Biophysical Society
DOI: 10.1016/j.bpj.2023.01.018

Epithelial cells adapt to curvature induction via transient active osmotic swelling

Autori: Caterina Tomba, Valeriy Luchnikov, Luca Barberi, Carles Blanch-Mercader, Aurélien Roux
Pubblicato in: Developmental Cell, 2022, ISSN 1534-5807
Editore: Cell Press
DOI: 10.1016/j.devcel.2022.04.017

Interfacial Inversion, Interference, and IR Absorption in Vibrational Sum Frequency Scattering Experiments

Autori: S. Pullanchery, L. Zhang, S. Kulik, and S. Roke
Pubblicato in: Journal Of Physical Chemistry B, 2023, ISSN 1520-6106
Editore: American Chemical Society
DOI: 10.1021/acs.jpcb.3c02727

Water as a contrast agent to quantify surface chemistry and physics using second harmonic scattering and imaging: A perspective

Autori: D. Roesel, M. Eremchev, T. Schönfeldová, S. Lee, S. Roke
Pubblicato in: Appl. Phys. Lett., 2022, ISSN 0003-6951
Editore: American Institute of Physics
DOI: 10.1063/5.0085807

Dissecting the membrane association mechanism of aerolysin pore at femtomolar concentrations using water as a probe

Autori: T. Roesel, C. Cao, JF. Bada Juarez, M. Dal Peraro, S. Roke
Pubblicato in: Nano Letters, 2024, ISSN 1530-6984
Editore: American Chemical Society
DOI: 10.1101/2024.01.18.576179

Liposomes and Lipid Droplets Display a Reversal of Charge-Induced Hydration Asymmetry

Autori: Saranya Pullanchery, Nathan Dupertuis, Tereza Roesel, Sylvie Roke
Pubblicato in: Nano Letters, 2023, ISSN 1530-6984
Editore: American Chemical Society
DOI: 10.1021/acs.nanolett.3c02653

Dynamic Second Harmonic Imaging of Proton Translocation Through Water Needles in Lipid Membranes

Autori: S. Lee, C. S. Poojari, A. Maznichenko, D. Roesel, I. Swiderska, P. Pohl, J. S. Hub, S. Roke
Pubblicato in: Journal of the American Chemical Society, 2024, ISSN 0066-4154
Editore: Annual Reviews, Inc.
DOI: 10.1021/jacs.4c02810

High throughput wide field second harmonic imaging of giant unilamellar vesicles

Autori: M. Eremchev, D. Roesel, P.-M. Dansette, A. Michailovas, S. Roke
Pubblicato in: Biointerphases, 2023, ISSN 1559-4106
Editore: American Vacuum Society
DOI: 10.1116/6.0002640

Mechanism for Vipp1 spiral formation, ring biogenesis, and membrane repair

Autori: Souvik Naskar, Andrea Merino, Javier Espadas, Jayanti Singh, Aurelien Roux, Adai Colom, Harry H. Low
Pubblicato in: Nature Structural & Molecular Biology, 2024, ISSN 1545-9993
Editore: Nature Publishing Group
DOI: 10.1038/s41594-024-01401-8

Pressure and curvature control of the cell cycle in epithelia growing under spherical confinement

Autori: Morphogenesis requires spatiotemporal regulation of proliferation, both by biochemical and mechanical cues. In epithelia, this regulation is called contact inhibition of proliferation, but disentangling biochemical from mechanical cues remains challenging. Here, we show that epithelia growing under confinement accumulate pressure that inhibits proliferation above a threshold value. During growth,
Pubblicato in: Cell Reports, 2022, ISSN 2211-1247
Editore: Cell Press
DOI: 10.1016/j.celrep.2022.111227