Description du projet
Un biocapteur de l’origami ADN
Les paires de bases complémentaires de l’ADN permettent qu’il se plie dans les formes et nanostructures souhaitées par le biais d’un processus appelé origami ADN. Le projet DeDNAed, financé par l’UE, travaille sur un nouveau capteur fondé sur un modèle d’origami ADN offrant sensibilité, versatilité et vitesse inégalées. L’architecture du biocapteur est essentiellement un brin simple d’ADN qui se plie après un traitement thermique et qui crée des extrémités collantes qui sont par la suite agrémentées à l’aide d’éléments de détection et de nanoparticules. Ce biocapteur ne se limite pas à des biomarqueurs spécifiques, offrant ainsi un vaste éventail d’applications potentielles, des technologies médicales à la surveillance alimentaire.
Objectif
"The project ""DeDNAed"" is intended to develop a novel, innovative biosensing platform whose advantages and benefits are in terms of sensitivity, versatility and being ultrafast by an optical approach. Our platform will be based on the assembly and integration of sensing elements (transducer and bioreceptor) by DNA origami. The DNA origami will serve as a “nano bread board” in order to precisely control the position of these elements and thus the sensor architecture at the nanometer scale.
Metallic atomic clusters (ACs) are integrated into a biological marker molecule (DNA or antibody) and thus represent the biological sensor element. This is specifically integrated into a nanoarray made of additional metallic nanoparticles precisely controlled by a DNA origami template and will lead to a significant increase in signal. DNA origami serves as an individually inter- and intramolecularly programmable nano bread board. A DNA origami consists of a single strand of DNA, folded by a thermal treatment and certain staple strands into any shapes (2D as well as 3D, dimensions between 10 and several 100 nanometers). So-called ""sticky ends"" on the surface of the DNA origami offer the possibility of an individual implementation of the sensing elements and NPs, by means of correspondingly complementary oligonucleotides with a resolutions of 2 nm. When the analyte is connected to the sensor element, a change in the Raman signal can thus be detected without major delay using surface-enhanced Raman spectroscopy (SERS). This sensor method is not bound to a specific biomarker molecule for the sensor element, but can be transferred to different marker molecules. This means a high degree of flexibility in the area of application, from medical technology to food monitoring. In addition, a transfer of the DNA origami-based sensor platform to flexible, textile substrates is carried out using lipid bi layers and the Langmuir-Blodgett method for later use as a wipe test or medical wearable."
Champ scientifique
- natural sciencesbiological sciencesgeneticsDNA
- natural sciencesbiological sciencesbiochemistrybiomoleculeslipids
- engineering and technologyelectrical engineering, electronic engineering, information engineeringelectronic engineeringsensors
- engineering and technologynanotechnologynano-materials
- natural sciencesphysical sciencesopticsspectroscopy
Mots‑clés
Programme(s)
Régime de financement
RIA - Research and Innovation actionCoordinateur
09111 Chemnitz
Allemagne