Descrizione del progetto
Un biosensore per l’Origami a DNA
Gli accoppiamenti di basi complementari del DNA consentono il suo ripiegamento nelle forme e nelle nanostrutture desiderate tramite un processo conosciuto come Origami a DNA. Il progetto DeDNAed, finanziato dall’UE, sta lavorando a un nuovo sensore basato su un modello di Origami a DNA che offre sensibilità, versatilità e rapidità senza eguali. L’architettura del biosensore è essenzialmente un filamento singolo di DNA che si piega a seguito del trattamento termico e che crea estremità appiccicose che vengono ulteriormente decorate utilizzando nanoparticelle ed elementi per il rilevamento. Il biosensore non è limitato a biomarcatori specifici, offrendo pertanto un sistema esteso di potenziali applicazioni: dalla tecnologia medica al monitoraggio di prodotti alimentari.
Obiettivo
"The project ""DeDNAed"" is intended to develop a novel, innovative biosensing platform whose advantages and benefits are in terms of sensitivity, versatility and being ultrafast by an optical approach. Our platform will be based on the assembly and integration of sensing elements (transducer and bioreceptor) by DNA origami. The DNA origami will serve as a “nano bread board” in order to precisely control the position of these elements and thus the sensor architecture at the nanometer scale.
Metallic atomic clusters (ACs) are integrated into a biological marker molecule (DNA or antibody) and thus represent the biological sensor element. This is specifically integrated into a nanoarray made of additional metallic nanoparticles precisely controlled by a DNA origami template and will lead to a significant increase in signal. DNA origami serves as an individually inter- and intramolecularly programmable nano bread board. A DNA origami consists of a single strand of DNA, folded by a thermal treatment and certain staple strands into any shapes (2D as well as 3D, dimensions between 10 and several 100 nanometers). So-called ""sticky ends"" on the surface of the DNA origami offer the possibility of an individual implementation of the sensing elements and NPs, by means of correspondingly complementary oligonucleotides with a resolutions of 2 nm. When the analyte is connected to the sensor element, a change in the Raman signal can thus be detected without major delay using surface-enhanced Raman spectroscopy (SERS). This sensor method is not bound to a specific biomarker molecule for the sensor element, but can be transferred to different marker molecules. This means a high degree of flexibility in the area of application, from medical technology to food monitoring. In addition, a transfer of the DNA origami-based sensor platform to flexible, textile substrates is carried out using lipid bi layers and the Langmuir-Blodgett method for later use as a wipe test or medical wearable."
Campo scientifico
- natural sciencesbiological sciencesgeneticsDNA
- natural sciencesbiological sciencesbiochemistrybiomoleculeslipids
- engineering and technologyelectrical engineering, electronic engineering, information engineeringelectronic engineeringsensors
- engineering and technologynanotechnologynano-materials
- natural sciencesphysical sciencesopticsspectroscopy
Parole chiave
Programma(i)
Invito a presentare proposte
Vedi altri progetti per questo bandoBando secondario
H2020-FETOPEN-2018-2019-2020-01
Meccanismo di finanziamento
RIA - Research and Innovation actionCoordinatore
09111 Chemnitz
Germania