Description du projet
Un modèle de rein humain bio-imprimé
L’insuffisance rénale aiguë (IRA) provoquée par des médicaments ou des virus peut aboutir à une maladie rénale chronique (MRC). Actuellement, on manque de modèles in vitro appropriés et pertinents pour étudier l’IRA et la MRC. Le projet BIRDIE, financé par l’UE, entend remédier à cette lacune en développant un modèle in vitro tubulo-interstitiel (TI) de rein humain en 3D à partir de cellules humaines primaires et de cellules souches pluripotentes induites. Les scientifiques emploieront des technologies de bio-impression et d’organes sur puce pour développer le modèle TI, qui reproduira fidèlement la physiologie du rein naturel. Le modèle BIRDIE devrait permettre d’étudier les maladies rénales et de tester des thérapies spécifiques aux patients.
Objectif
Eight hundred and fifty million people worldwide are currently affected by chronic kidney disease (CKD), which is also the 11th leading cause of mortality worldwide. CKD can occur from a multitude of causes including diabetes and high-blood pressure. Moreover, recent clinical and experimental studies have shown that CKD is closely interconnected with acute kidney injury (AKI) as well. Currently available in vitro models show limited relevance to study AKI, especially drug- and virus- induced AKI, due to the poor functionality and relevance compared to a diseased human tissue. BIRDIE aims at developing three-dimensional (3D) in vitro human renal tubulointerstitium (TI) models to enable viral infection and nephrotoxicity studies while creating a robust platform to address other diseases and treatment innovations in the future. Two enabling technologies, bioprinting and organ-on-chip, will be combined to build a microphysiological relevant TI model. Primary human cells and induced pluripotent stem cells will be used to generate kidney models, and combined with the aforementioned techniques model envisioning a reliable screening platform for future patient specific therapies. Our ambition is to create a new 3D renal in vitro model allowing an unprecedented degree of mimicry and function compared to a human kidney. While developing the model focusing on the applications mentioned before, our goal is to make it broadly applicable to the multitude of kidney-related diseases.
Champ scientifique
- social sciencessociologydemographymortality
- medical and health sciencesclinical medicineendocrinologydiabetes
- medical and health sciencesmedical biotechnologycells technologiesstem cells
- engineering and technologyother engineering and technologiesmicrotechnologyorgan on a chip
- medical and health sciencesclinical medicinenephrologykidney diseases
Mots‑clés
Programme(s)
Régime de financement
RIA - Research and Innovation actionCoordinateur
6200 MD Maastricht
Pays-Bas