TOXicity assessment on neurons and cardiomyocytes by means of FluoRescence Emitting Electrodes

Project Information

TOX-Free

Grant agreement ID: 964518

DOI

10.3030/964518

Project closed

EC signature date 16 December 2020

Start date 1 June 2021

End date 31 May 2025

Funded under

• EXCELLENT SCIENCE - Future and Emerging Technologies (FET)

Total cost

€ 3 035 166,25

EU contribution

€ 3 035 166,25

Coordinated by

FONDAZIONE ISTITUTO ITALIANO DI TECNOLOGIA
Italy

CORDIS provides links to public deliverables and publications of HORIZON projects.

Links to deliverables and publications from FP7 projects, as well as links to some specific result types such as dataset and software, are dynamically retrieved from OpenAIRE .

Deliverables

Websites, patent fillings, videos etc. (1)

Project website and logo

Documents, reports (1)

Report on the readout capabilities of VICE 1.0 with neurons

The report will include experimental data on the readout capabilities of VICE 10 with neuronal cultures

Publications

Peer reviewed articles (39)

Label-Free Detection of Biochemical Changes during Cortical Organoid Maturation via Raman Spectroscopy and Machine Learning

Author(s): Giulia Bruno, Michal Lipinski, Koseki J. Kobayashi-Kirschvink, Christian Tentellino, Peter T. C. So, Jeon Woong Kang, Francesco De Angelis
Published in: Analytical Chemistry, Issue 97, 2025, Page(s) 5029-5037, ISSN 0003-2700
Publisher: American Chemical Society
DOI: 10.1021/acs.analchem.4c05661

On the usefulness of animals as a model system (part II): Considering benefits within distinct use domains

Author(s): Pallocca, Giorgia; Leist, Marcel
Published in: ALTEX- Alternatives to Animal Experimentation, 2022, ISSN 1868-8551
Publisher: ALTEX Edition
DOI: 10.14573/altex.2207111

New approach methods to assess developmental and adult neurotoxicity for regulatory use: a PARC work package 5 project

Author(s): Tamara Tal, Oddvar Myhre, Ellen Fritsche, Joëlle Rüegg, Kai Craenen, Kiara Aiello-Holden, Caroline Agrillo, Patrick J. Babin, Beate I. Escher, Hubert Dirven, Kati Hellsten, Kristine Dolva, Ellen Hessel, Harm J. Heusinkveld, Yavor Hadzhiev, Selma Hurem, Karolina Jagiello, Beata Judzinska, Nils Klüver, Anja Knoll-Gellida, Britta A. Kühne, Marcel Leist, Malene Lislien, Jan L. Lyche, Ferenc Mülle
Published in: Frontiers in Toxicology, Issue 6, 2024, ISSN 2673-3080
Publisher: Frontiers
DOI: 10.3389/ftox.2024.1359507

Dynamic Metabolic and Transcriptional Responses of Proteasome-Inhibited Neurons

Author(s): Ilinca Suciu; Johannes Delp; Simon Gutbier; Anna-Katharina Ückert; Anna-Sophie Spreng; Philipp Eberhard; Christiaan Karreman; Falk Schreiber; Katrin Madjar; Jörg Rahnenführer; Ivana Celardo; Ivano Amelio; Marcel Leist
Published in: Antioxidants, 2023, ISSN 2076-3921
Publisher: MDPI
DOI: 10.3390/antiox12010164

Leveraging biomarkers and translational medicine for preclinical safety - Lessons for advancing the validation of alternatives to animal testing

Author(s): Thomas Hartung , Nicholas M. P. King, Nicole Kleinstreuer, Marcel Leist, Danilo A. Tagle
Published in: ALTEX, Issue 41, 2024, ISSN 1868-596X
Publisher: ALTEX Edition
DOI: 10.14573/altex.2410011

Revolutionizing developmental neurotoxicity testing – a journey from animal models to advanced in vitro systems

Author(s): Lena Smirnova, Helena T. Hogberg, Marcel Leist, Thomas Hartung
Published in: ALTEX, Issue Vol. 41 No. 2, 2024, Page(s) 152–178, ISSN 1868-596X
Publisher: ALTEX Edition
DOI: 10.14573/altex.2403281

High Accuracy Classification of Developmental Toxicants by In Vitro Tests of Human Neuroepithelial and Cardiomyoblast Differentiation.

Author(s): Florian Seidel; Anna Cherianidou; Franziska Kappenberg; Miriam Marta; Nadine Dreser; Jonathan Blum; Tanja Waldmann; Nils Blüthgen; Johannes Meisig; Katrin Madjar; Margit Henry; Tamara Rotshteyn; Andreas Scholtz-Illigens; Rosemarie Marchan; Karolina Edlund; Marcel Leist; Jörg Rahnenführer; Agapios Sachinidis; Jan Georg Hengstler
Published in: Cells, Issue 11, 2022, Page(s) 3404, ISSN 2073-4409
Publisher: MDPI
DOI: 10.3390/cells11213404

Analysis of health concerns not addressed by REACH for low tonnage chemicals and opportunities for new approach methodology

Author(s): Philip Botham; Mark T. D. Cronin; Richard Currie; John Doe; Dorothee Funk-Weyer; Timothy W. Gant; Marcel Leist; Sue Marty; Bennard van Ravenzwaay; Carl Westmoreland
Published in: Archives of Toxicology, 2023, ISSN 0340-5761
Publisher: Springer Verlag
DOI: 10.1007/s00204-023-03601-5

Distinct and Dynamic Transcriptome Adaptations of iPSC-Generated Astrocytes after Cytokine Stimulation

Author(s): Anna-Sophie Spreng; Markus Brüll; Heidrun Leisner; Ilinca Suciu; Marcel Leist
Published in: Cells, Issue Volume 11; Issue 17; Pages: 2644, 2022, ISSN 2073-4409
Publisher: MDPI
DOI: 10.3390/cells11172644

Electrode- and Label-Free Assessment of Electrophysiological Firing Rates through Cytochrome C Monitoring via Raman Spectroscopy

Author(s): Christian Tentellino, Marta d’Amora, Rustamzhon Melikov, Giuseppina Iachetta, Giulia Bruno, Francesco Tantussi, Michele Dipalo, Francesco De Angelis
Published in: ACS Sensors, Issue 10, 2025, Page(s) 1228-1236, ISSN 2379-3694
Publisher: American Chemical Society (ACS)
DOI: 10.1021/acssensors.4c03133

Assay for evaluation of proarrhythmic effects of herbal products: Case study with 12 Evodia preparations

Author(s): Bozhidar Baltov; Stanislav Beyl; Igor Baburin; Jakob Reinhardt; Phillip Szkokan; Aleksandra Garifulina; Eugen Timin; Udo Kraushaar; Olivier Potterat; Matthias Hamburger; Philipp Kügler; Steffen Hering
Published in: Toxicology Reports, Issue 2, 2023, ISSN 2214-7500
Publisher: Elsevier Scientific Publishers Ireland
DOI: 10.1016/j.toxrep.2023.04.014

Modeling ferroptosis in human dopaminergic neurons: Pitfalls and opportunities for neurodegeneration research

Author(s): Nadine Renner, Franziska Schöb, Regina Pape, Ilinca Suciu, Anna-Sophie Spreng, Anna-Katharina Ückert, Eike Cöllen, Federica Bovio, Bruno Chilian, Johannes Bauer, Stefan Röpcke, Jörg Bergemann, Marcel Leist, Stefan Schildknecht
Published in: Redox Biology, Issue 73, 2024, Page(s) 103165, ISSN 2213-2317
Publisher: Elsevier BV
DOI: 10.1016/j.redox.2024.103165

Synuclein Family Members Prevent Membrane Damage by Counteracting α-Synuclein Aggregation.

Author(s): Christian Scheibe; Christiaan Karreman; Stefan Schildknecht; Marcel Leist; Karin Hauser
Published in: biomolecules, Issue Issue 8, 2021, Page(s) 1067, ISSN 2218-273X
Publisher: mdpi
DOI: 10.3390/biom11081067

Laser-Induced Action Potential-Like Measurements of Cardiomyocytes on Microelectrode Arrays for Increased Predictivity of Safety Pharmacology

Author(s): Udo Kraushaar; Karin Gebhardt; Timm Danker; Jasmin Schaefer
Published in: JoVE, 2022, ISSN 1940-087X
Publisher: MYJoVE Corporation
DOI: 10.3791/64355

Optimization of the TeraTox assay for preclinical teratogenicity assessment

Author(s): Manuela Jaklin; Jitao David Zhang; Nicole Schaefer; Nicole Clemann; Paul Barrow; Erich Kueng; Lisa Sach-Peltason; Claudia McGinnis; Marcel Leist; Stefan Kustermann
Published in: Toxicological Sciences, Issue Volume 188, Issue 1, 2022, Page(s) 17–33, ISSN 1096-6080
Publisher: Oxford University Press
DOI: 10.1101/2021.07.06.451364

Preparation of Viable Human Neurites for Neurobiological and Neurodegeneration Studies

Author(s): Markus Brüll, Nils Geese, Ivana Celardo, Michael Laumann, Marcel Leist
Published in: Cells, Issue 13, 2025, Page(s) 242, ISSN 2073-4409
Publisher: MDPI
DOI: 10.3390/cells13030242

Generation of Human Nociceptor-Enriched Sensory Neurons for the Study of Pain-Related Dysfunctions

Author(s): Anna-Katharina Holzer; Christiaan Karreman; Ilinca Suciu; Lara-Seline Furmanowsky; Harald Wohlfarth; Dominik Loser; Wilhelm G Dirks; Emilio Pardo González; Marcel Leist
Published in: Stem Cells Transl Med., Issue Volume 11, Issue 7, 2022, Page(s) 727–741, ISSN 2157-6564
Publisher: AlphaMed Press
DOI: 10.1101/2022.02.19.480828

Probing ND7/23 neuronal cells before and after differentiation with SERS using Sharp-tipped Au nanopyramid arrays

Author(s): Marzia Iarossi, Aliaksandr Hubarevich, Giuseppina Iachetta, MicheleDipalo, Jian-An Huang, Daniel Darvill, Francesco De Angelis
Published in: Sensors and Actuators B: Chemical, Issue Volume 361, 2022, Page(s) 131724, ISSN 0925-4005
Publisher: Elsevier BV
DOI: 10.1016/j.snb.2022.131724

Definition of the Neurotoxicity-Associated Metabolic Signature Triggered by Berberine and Other Respiratory Chain Inhibitors

Author(s): Ilinca Suciu, Johannes Delp, Simon Gutbier, Julian Suess, Lars Henschke, Ivana Celardo, Thomas U. Mayer, Ivano Amelio, Marcel Leist
Published in: Antioxidants, Issue 13, 2025, Page(s) 49, ISSN 2076-3921
Publisher: MDPI
DOI: 10.3390/antiox13010049

Establishment of a Human Cell-Based in Vitro Battery to Assess Developmental Neurotoxicity Hazard of Chemicals

Author(s): Jonathan Blum; Stefan Masjosthusmann; Kristina Bartmann; Farina Bendt; Xenia Dolde; Arif Dönmez; Nils Förster; Anna-Katharina Holzer; Ulrike Hübenthal; Hagen Eike Keßel; Sadiye Kilic; Jördis Klose; Melanie Pahl; Lynn-Christin Stürzl; Iris Mangas; Andrea Terron; Kevin M. Crofton; Martin Scholze; Axel Mosig; Marcel Leist; Ellen Fritsche
Published in: Chemosphere, 2023, ISSN 0045-6535
Publisher: Pergamon Press Ltd.
DOI: 10.2139/ssrn.4161670

Specific Attenuation of Purinergic Signaling during Bortezomib-Induced Peripheral Neuropathy In Vitro

Author(s): Anna-Katharina Holzer; Ilinca Suciu; Christiaan Karreman; Thomas Goj; Marcel Leist
Published in: International Journal of Molecular Sciences, 2022, ISSN 1422-0067
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms23073734

Elements and development processes for test methods in toxicology and human health-relevant life science research

Author(s): Cöllen, Eike; Tanaskov, Yaroslav; Holzer, Anna-Katharina; Dipalo, Michele; Schäfer, Jasmin; Kraushaar, Udo; Leist, Marcel
Published in: ALTEX, Issue 8, 2024, ISSN 1868-596X
Publisher: Altex Edition

On the usefulness of animals as a model system (part I): Overview of criteria and focus on robustness

Author(s): Pallocca, Giorgia; Rovida, Costanza; Leist, Marcel
Published in: Altex, 2022, ISSN 1868-8551
Publisher: ALTEX Edition
DOI: 10.14573/altex.2203291

Identification of the bacterial metabolite aerugine as potential trigger of human dopaminergic neurodegeneration

Author(s): Anna-Katharina Ückert, Sina Rütschlin, Simon Gutbier, Nathalie Christine Wörz, Mahfuzur R. Miah, Airton C. Martins, Isa Hauer, Anna-Katharina Holzer, Birthe Meyburg, Ann-Kathrin Mix, Christof Hauck, Michael Aschner, Thomas Böttcher, Marcel Leist
Published in: Environment International, Issue 180, 2023, Page(s) 108229, ISSN 0160-4120
Publisher: Pergamon Press Ltd.
DOI: 10.1016/j.envint.2023.108229

A framework for chemical safety assessment incorporating new approach methodologies within REACH

Author(s): Nicholas Ball, Remi Bars, Philip A. Botham, Andreea Cuciureanu, Mark T. D. Cronin, John E. Doe, Tatsiana Dudzina, Timothy W. Gant, Marcel Leist, Bennard van Ravenzwaay
Published in: Archives of Toxicology, Issue 96, 2022, Page(s) 743–766, ISSN 0340-5761
Publisher: Springer Verlag
DOI: 10.1007/s00204-021-03215-9

Direct recording of action potentials of cardiomyocytes through solution processed planar electrolyte-gated field-effect transistors

Author(s): Adrica Kyndiah, Michele Dipalo, Alireza Molazemhosseini, Fabrizio Antonio Viola, Francesco Modena, Giuseppina Iachetta, Nicolas F. Zorn, Felix J. Berger, Jana Zaumseil, Mario Caironi, Francesco De Angelis
Published in: Sensors and Actuators B: Chemical, Issue Volume 393, 134227, 2023, ISSN 0925-4005
Publisher: Elsevier BV
DOI: 10.1016/j.snb.2023.134227

Programmed neurite degeneration in human central nervous system neurons driven by changes in NAD+ metabolism

Author(s): Markus Brüll, Selina Multrus, Michael Schäfer, Ivana Celardo, Christiaan Karreman, Marcel Leist
Published in: Cell Death & Disease, Issue 16, 2025, ISSN 2041-4889
Publisher: Nature Publishing Group
DOI: 10.1038/s41419-024-07326-w

Passive Recording of Bioelectrical Signals from Non-Excitable Cells by Fluorescent Mirroring

Author(s): Rosalia Moreddu, Alessio Boschi, Marta d’Amora, Aliaksandr Hubarevich, Michele Dipalo*, and Francesco De Angelis*
Published in: Nano Letters, Issue 2023, 23, 8, 2023, Page(s) 3217–3223, ISSN 1530-6984
Publisher: American Chemical Society
DOI: 10.1021/acs.nanolett.2c05053

Inhibition of Neural Crest Cell Migration by Strobilurin Fungicides and Other Mitochondrial Toxicants

Author(s): Viktoria Magel, Jonathan Blum, Xenia Dolde, Heidrun Leisner, Karin Grillberger, Hiba Khalidi, Iain Gardner, Gerhard F. Ecker, Giorgia Pallocca, Nadine Dreser, Marcel Leist
Published in: Cells, Issue 13, 2024, Page(s) 2057, ISSN 2073-4409
Publisher: MDPI
DOI: 10.3390/cells13242057

Long-term in vitro recording of cardiac action potentials on microelectrode arrays for chronic cardiotoxicity assessment

Author(s): Giuseppina Iachetta, Giovanni Melle, Nicolò Colistra, Francesco Tantussi, Francesco De Angelis & Michele Dipalo
Published in: Archives of Toxicology, Issue 97, 2023, Page(s) 509–522, ISSN 0340-5761
Publisher: Springer Verlag
DOI: 10.1007/s00204-022-03422-y

Classification of Developmental Toxicants in a Human iPSC Transcriptomics-Based Test

Author(s): Anna Cherianidou; Florian Seidel; Franziska Kappenberg; Nadine Dreser; Jonathan Blum; Tanja Waldmann; Nils Blüthgen; Johannes Meisig; Katrin Madjar; Margit Henry; Tamara Rotshteyn; Rosemarie Marchan; Karolina Edlund; Marcel Leist; Jörg Rahnenführer; Agapios Sachinidis; Jan G. Hengstler
Published in: Chemical Research in Toxicology, Issue 35, 5, 2022, Page(s) 760–773, ISSN 0893-228X
Publisher: American Chemical Society
DOI: 10.1021/acs.chemrestox.1c00392

The long way from raw data to NAM-based information: Overview on data layers and processing steps

Author(s): Jonathan Blum , Markus Brüll, Jan G. Hengstler, Daniel R. Dietrich, Andreas J. Gruber, Michele Dipalo, Udo Kraushaar, Iris Mangas, Andrea Terron, Ellen Fritsche, Philip Marx-Stoelting, Barry Hardy, Andreas Schepky, Sylvia Escher, Thomas Hartung, Robert Landsiedel, Alex Odermatt, Magdalini Sachana, Katharina Koch, Arif Dönmez, Stefan Masjosthusmann, Kathrin Bothe, Stefan Schildknecht, Mario Beil
Published in: ALTEX, Issue 42, 2025, ISSN 1868-596X
Publisher: ALTEX Edition
DOI: 10.14573/altex.2412171

Chemical and Biological Mechanisms Relevant to the Rescue of MG-132-Treated Neurons by Cysteine

Author(s): Anna-Katharina Ückert, Ilinca Suciu, Anja Land, Anna-Sophie Spreng, Hannah Welte, Doreen Herzog, Michael Basler, Marcel Leist
Published in: Antioxidants, Issue 14, 2025, Page(s) 128, ISSN 2076-3921
Publisher: MDPI AG
DOI: 10.3390/antiox14020128

G × E interactions as a basis for toxicological uncertainty

Author(s): Ilinca Suciu; David Pamies; Roberta Peruzzo; Petra H. Wirtz; Lena Smirnova; Giorgia Pallocca; Christof Hauck; Mark T. D. Cronin; Jan G. Hengstler; Thomas Brunner; Thomas Hartung; Ivano Amelio; Marcel Leist
Published in: Archives of Toxicology, Issue 97, 2023, Page(s) 2035–2049, ISSN 0340-5761
Publisher: Springer Verlag
DOI: 10.1007/s00204-023-03500-9

Circulating (Poly)phenol Metabolites: Neuroprotection in a 3D Cell Model of Parkinson's Disease

Author(s): Carecho, Rafael; Figueira, Inês; Terrasso, Ana Paula; Godinho‐Pereira, Joana; de Oliveira Sequeira, Catarina; Pereira, Sofia Azeredo; Milenkovic, Dragan; Leist, Marcel; Brito, Catarina; Nunes dos Santos, Cláudia
Published in: Molecular Nutrition & Food Research, Issue 2100959, 2022, ISSN 1613-4125
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/mnfr.202100959

Profiling of Human Neural Crest Chemoattractant Activity as a Replacement of Fetal Bovine Serum for In Vitro Chemotaxis Assays

Author(s): Xenia Dolde; Christiaan Karreman; Marianne Wiechers; Stefan Schildknecht; Marcel Leist
Published in: International Journal of Molecular Sciences, Vol 22, Iss 10079, p 10079 (2021), Issue 22(18), 2021, Page(s) 10079, ISSN 1422-0067
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms221810079

Recommendations on fit-for-purpose criteria to establish quality management for microphysiological systems and for monitoring their reproducibility

Author(s): David Pamies, Jason Ekert, Marie-Gabrielle Zurich, Olivier Frey, Sophie Werner, Monica Piergiovanni, Benjamin S. Freedman, Adrian Kee Keong Teo, Hendrik Erfurth, Darwin R. Reyes, Peter Loskill, Pelin Candarlioglu, Laura Suter-Dick, Shan Wang, Thomas Hartung, Sandra Coecke, Glyn N. Stacey, Beren Atac Wagegg, Eva-Maria Dehne, Francesca Pistollato, Marcel Leist
Published in: Stem Cell Reports, Issue 19, 2024, Page(s) 604-617, ISSN 2213-6711
Publisher: Cell Press
DOI: 10.1016/j.stemcr.2024.03.009

Acute effects of the imidacloprid metabolite desnitro-imidacloprid on human nACh receptors relevant for neuronal signaling.

Author(s): Dominik Loser; Dominik Loser; Karin Grillberger; Maria G. Hinojosa; Jonathan Blum; Yves Haufe; Timm Danker; Ylva Johansson; Clemens Möller; Annette Nicke; Susanne Hougaard Bennekou; Iain Gardner; Caroline Bauch; Paul Walker; Anna Forsby; Gerhard F. Ecker; Udo Kraushaar; Marcel Leist
Published in: Archives of Toxicology, Issue 95, 2021, Page(s) 3695–3716, ISSN 0340-5761
Publisher: Springer Verlag
DOI: 10.1007/s00204-021-03168-z

Longitudinal and Noninvasive Intracellular Recordings of Spontaneous Electrophysiological Activity in Rat Primary Neurons on Planar MEA Electrodes

Author(s): Rustamzhon Melikov, Giuseppina Iachetta, Marta d'Amora, Giovanni Melle, Silvia Conti, Francesco Tantussi, Michele Dipalo, Francesco De Angelis
Published in: Advanced Materials, Issue 2412697, 2025, ISSN 0935-9648
Publisher: United Nations Industrial Developement Organization
DOI: 10.1002/adma.202412697