TOXicity assessment on neurons and cardiomyocytes by means of FluoRescence Emitting Electrodes

Informations projet

TOX-Free

N° de convention de subvention: 964518

DOI

10.3030/964518

Projet clôturé

Date de signature de la CE 16 Decembre 2020

Date de début 1 Juin 2021

Date de fin 31 Mai 2025

Financé au titre de

• EXCELLENT SCIENCE - Future and Emerging Technologies (FET)

Coût total

€ 3 035 166,25

Contribution de l’UE

€ 3 035 166,25

Coordonné par

FONDAZIONE ISTITUTO ITALIANO DI TECNOLOGIA
Italy

CORDIS fournit des liens vers les livrables publics et les publications des projets HORIZON.

Les liens vers les livrables et les publications des projets du 7e PC, ainsi que les liens vers certains types de résultats spécifiques tels que les jeux de données et les logiciels, sont récupérés dynamiquement sur OpenAIRE .

Livrables

Websites, patent fillings, videos etc. (1)

Project website and logo

Documents, reports (1)

Report on the readout capabilities of VICE 1.0 with neurons

The report will include experimental data on the readout capabilities of VICE 10 with neuronal cultures

Publications

Peer reviewed articles (39)

Label-Free Detection of Biochemical Changes during Cortical Organoid Maturation via Raman Spectroscopy and Machine Learning

Auteurs: Giulia Bruno, Michal Lipinski, Koseki J. Kobayashi-Kirschvink, Christian Tentellino, Peter T. C. So, Jeon Woong Kang, Francesco De Angelis
Publié dans: Analytical Chemistry, Numéro 97, 2025, Page(s) 5029-5037, ISSN 0003-2700
Éditeur: American Chemical Society
DOI: 10.1021/acs.analchem.4c05661

On the usefulness of animals as a model system (part II): Considering benefits within distinct use domains

Auteurs: Pallocca, Giorgia; Leist, Marcel
Publié dans: ALTEX- Alternatives to Animal Experimentation, 2022, ISSN 1868-8551
Éditeur: ALTEX Edition
DOI: 10.14573/altex.2207111

New approach methods to assess developmental and adult neurotoxicity for regulatory use: a PARC work package 5 project

Auteurs: Tamara Tal, Oddvar Myhre, Ellen Fritsche, Joëlle Rüegg, Kai Craenen, Kiara Aiello-Holden, Caroline Agrillo, Patrick J. Babin, Beate I. Escher, Hubert Dirven, Kati Hellsten, Kristine Dolva, Ellen Hessel, Harm J. Heusinkveld, Yavor Hadzhiev, Selma Hurem, Karolina Jagiello, Beata Judzinska, Nils Klüver, Anja Knoll-Gellida, Britta A. Kühne, Marcel Leist, Malene Lislien, Jan L. Lyche, Ferenc Mülle
Publié dans: Frontiers in Toxicology, Numéro 6, 2024, ISSN 2673-3080
Éditeur: Frontiers
DOI: 10.3389/ftox.2024.1359507

Dynamic Metabolic and Transcriptional Responses of Proteasome-Inhibited Neurons

Auteurs: Ilinca Suciu; Johannes Delp; Simon Gutbier; Anna-Katharina Ückert; Anna-Sophie Spreng; Philipp Eberhard; Christiaan Karreman; Falk Schreiber; Katrin Madjar; Jörg Rahnenführer; Ivana Celardo; Ivano Amelio; Marcel Leist
Publié dans: Antioxidants, 2023, ISSN 2076-3921
Éditeur: MDPI
DOI: 10.3390/antiox12010164

Leveraging biomarkers and translational medicine for preclinical safety - Lessons for advancing the validation of alternatives to animal testing

Auteurs: Thomas Hartung , Nicholas M. P. King, Nicole Kleinstreuer, Marcel Leist, Danilo A. Tagle
Publié dans: ALTEX, Numéro 41, 2024, ISSN 1868-596X
Éditeur: ALTEX Edition
DOI: 10.14573/altex.2410011

Revolutionizing developmental neurotoxicity testing – a journey from animal models to advanced in vitro systems

Auteurs: Lena Smirnova, Helena T. Hogberg, Marcel Leist, Thomas Hartung
Publié dans: ALTEX, Numéro Vol. 41 No. 2, 2024, Page(s) 152–178, ISSN 1868-596X
Éditeur: ALTEX Edition
DOI: 10.14573/altex.2403281

High Accuracy Classification of Developmental Toxicants by In Vitro Tests of Human Neuroepithelial and Cardiomyoblast Differentiation.

Auteurs: Florian Seidel; Anna Cherianidou; Franziska Kappenberg; Miriam Marta; Nadine Dreser; Jonathan Blum; Tanja Waldmann; Nils Blüthgen; Johannes Meisig; Katrin Madjar; Margit Henry; Tamara Rotshteyn; Andreas Scholtz-Illigens; Rosemarie Marchan; Karolina Edlund; Marcel Leist; Jörg Rahnenführer; Agapios Sachinidis; Jan Georg Hengstler
Publié dans: Cells, Numéro 11, 2022, Page(s) 3404, ISSN 2073-4409
Éditeur: MDPI
DOI: 10.3390/cells11213404

Analysis of health concerns not addressed by REACH for low tonnage chemicals and opportunities for new approach methodology

Auteurs: Philip Botham; Mark T. D. Cronin; Richard Currie; John Doe; Dorothee Funk-Weyer; Timothy W. Gant; Marcel Leist; Sue Marty; Bennard van Ravenzwaay; Carl Westmoreland
Publié dans: Archives of Toxicology, 2023, ISSN 0340-5761
Éditeur: Springer Verlag
DOI: 10.1007/s00204-023-03601-5

Distinct and Dynamic Transcriptome Adaptations of iPSC-Generated Astrocytes after Cytokine Stimulation

Auteurs: Anna-Sophie Spreng; Markus Brüll; Heidrun Leisner; Ilinca Suciu; Marcel Leist
Publié dans: Cells, Numéro Volume 11; Numéro 17; Pages: 2644, 2022, ISSN 2073-4409
Éditeur: MDPI
DOI: 10.3390/cells11172644

Electrode- and Label-Free Assessment of Electrophysiological Firing Rates through Cytochrome C Monitoring via Raman Spectroscopy

Auteurs: Christian Tentellino, Marta d’Amora, Rustamzhon Melikov, Giuseppina Iachetta, Giulia Bruno, Francesco Tantussi, Michele Dipalo, Francesco De Angelis
Publié dans: ACS Sensors, Numéro 10, 2025, Page(s) 1228-1236, ISSN 2379-3694
Éditeur: American Chemical Society (ACS)
DOI: 10.1021/acssensors.4c03133

Assay for evaluation of proarrhythmic effects of herbal products: Case study with 12 Evodia preparations

Auteurs: Bozhidar Baltov; Stanislav Beyl; Igor Baburin; Jakob Reinhardt; Phillip Szkokan; Aleksandra Garifulina; Eugen Timin; Udo Kraushaar; Olivier Potterat; Matthias Hamburger; Philipp Kügler; Steffen Hering
Publié dans: Toxicology Reports, Numéro 2, 2023, ISSN 2214-7500
Éditeur: Elsevier Scientific Publishers Ireland
DOI: 10.1016/j.toxrep.2023.04.014

Modeling ferroptosis in human dopaminergic neurons: Pitfalls and opportunities for neurodegeneration research

Auteurs: Nadine Renner, Franziska Schöb, Regina Pape, Ilinca Suciu, Anna-Sophie Spreng, Anna-Katharina Ückert, Eike Cöllen, Federica Bovio, Bruno Chilian, Johannes Bauer, Stefan Röpcke, Jörg Bergemann, Marcel Leist, Stefan Schildknecht
Publié dans: Redox Biology, Numéro 73, 2024, Page(s) 103165, ISSN 2213-2317
Éditeur: Elsevier BV
DOI: 10.1016/j.redox.2024.103165

Synuclein Family Members Prevent Membrane Damage by Counteracting α-Synuclein Aggregation.

Auteurs: Christian Scheibe; Christiaan Karreman; Stefan Schildknecht; Marcel Leist; Karin Hauser
Publié dans: biomolecules, Numéro Numéro 8, 2021, Page(s) 1067, ISSN 2218-273X
Éditeur: mdpi
DOI: 10.3390/biom11081067

Laser-Induced Action Potential-Like Measurements of Cardiomyocytes on Microelectrode Arrays for Increased Predictivity of Safety Pharmacology

Auteurs: Udo Kraushaar; Karin Gebhardt; Timm Danker; Jasmin Schaefer
Publié dans: JoVE, 2022, ISSN 1940-087X
Éditeur: MYJoVE Corporation
DOI: 10.3791/64355

Optimization of the TeraTox assay for preclinical teratogenicity assessment

Auteurs: Manuela Jaklin; Jitao David Zhang; Nicole Schaefer; Nicole Clemann; Paul Barrow; Erich Kueng; Lisa Sach-Peltason; Claudia McGinnis; Marcel Leist; Stefan Kustermann
Publié dans: Toxicological Sciences, Numéro Volume 188, Numéro 1, 2022, Page(s) 17–33, ISSN 1096-6080
Éditeur: Oxford University Press
DOI: 10.1101/2021.07.06.451364

Preparation of Viable Human Neurites for Neurobiological and Neurodegeneration Studies

Auteurs: Markus Brüll, Nils Geese, Ivana Celardo, Michael Laumann, Marcel Leist
Publié dans: Cells, Numéro 13, 2025, Page(s) 242, ISSN 2073-4409
Éditeur: MDPI
DOI: 10.3390/cells13030242

Generation of Human Nociceptor-Enriched Sensory Neurons for the Study of Pain-Related Dysfunctions

Auteurs: Anna-Katharina Holzer; Christiaan Karreman; Ilinca Suciu; Lara-Seline Furmanowsky; Harald Wohlfarth; Dominik Loser; Wilhelm G Dirks; Emilio Pardo González; Marcel Leist
Publié dans: Stem Cells Transl Med., Numéro Volume 11, Numéro 7, 2022, Page(s) 727–741, ISSN 2157-6564
Éditeur: AlphaMed Press
DOI: 10.1101/2022.02.19.480828

Probing ND7/23 neuronal cells before and after differentiation with SERS using Sharp-tipped Au nanopyramid arrays

Auteurs: Marzia Iarossi, Aliaksandr Hubarevich, Giuseppina Iachetta, MicheleDipalo, Jian-An Huang, Daniel Darvill, Francesco De Angelis
Publié dans: Sensors and Actuators B: Chemical, Numéro Volume 361, 2022, Page(s) 131724, ISSN 0925-4005
Éditeur: Elsevier BV
DOI: 10.1016/j.snb.2022.131724

Definition of the Neurotoxicity-Associated Metabolic Signature Triggered by Berberine and Other Respiratory Chain Inhibitors

Auteurs: Ilinca Suciu, Johannes Delp, Simon Gutbier, Julian Suess, Lars Henschke, Ivana Celardo, Thomas U. Mayer, Ivano Amelio, Marcel Leist
Publié dans: Antioxidants, Numéro 13, 2025, Page(s) 49, ISSN 2076-3921
Éditeur: MDPI
DOI: 10.3390/antiox13010049

Establishment of a Human Cell-Based in Vitro Battery to Assess Developmental Neurotoxicity Hazard of Chemicals

Auteurs: Jonathan Blum; Stefan Masjosthusmann; Kristina Bartmann; Farina Bendt; Xenia Dolde; Arif Dönmez; Nils Förster; Anna-Katharina Holzer; Ulrike Hübenthal; Hagen Eike Keßel; Sadiye Kilic; Jördis Klose; Melanie Pahl; Lynn-Christin Stürzl; Iris Mangas; Andrea Terron; Kevin M. Crofton; Martin Scholze; Axel Mosig; Marcel Leist; Ellen Fritsche
Publié dans: Chemosphere, 2023, ISSN 0045-6535
Éditeur: Pergamon Press Ltd.
DOI: 10.2139/ssrn.4161670

Specific Attenuation of Purinergic Signaling during Bortezomib-Induced Peripheral Neuropathy In Vitro

Auteurs: Anna-Katharina Holzer; Ilinca Suciu; Christiaan Karreman; Thomas Goj; Marcel Leist
Publié dans: International Journal of Molecular Sciences, 2022, ISSN 1422-0067
Éditeur: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms23073734

Elements and development processes for test methods in toxicology and human health-relevant life science research

Auteurs: Cöllen, Eike; Tanaskov, Yaroslav; Holzer, Anna-Katharina; Dipalo, Michele; Schäfer, Jasmin; Kraushaar, Udo; Leist, Marcel
Publié dans: ALTEX, Numéro 8, 2024, ISSN 1868-596X
Éditeur: Altex Edition

On the usefulness of animals as a model system (part I): Overview of criteria and focus on robustness

Auteurs: Pallocca, Giorgia; Rovida, Costanza; Leist, Marcel
Publié dans: Altex, 2022, ISSN 1868-8551
Éditeur: ALTEX Edition
DOI: 10.14573/altex.2203291

Identification of the bacterial metabolite aerugine as potential trigger of human dopaminergic neurodegeneration

Auteurs: Anna-Katharina Ückert, Sina Rütschlin, Simon Gutbier, Nathalie Christine Wörz, Mahfuzur R. Miah, Airton C. Martins, Isa Hauer, Anna-Katharina Holzer, Birthe Meyburg, Ann-Kathrin Mix, Christof Hauck, Michael Aschner, Thomas Böttcher, Marcel Leist
Publié dans: Environment International, Numéro 180, 2023, Page(s) 108229, ISSN 0160-4120
Éditeur: Pergamon Press Ltd.
DOI: 10.1016/j.envint.2023.108229

A framework for chemical safety assessment incorporating new approach methodologies within REACH

Auteurs: Nicholas Ball, Remi Bars, Philip A. Botham, Andreea Cuciureanu, Mark T. D. Cronin, John E. Doe, Tatsiana Dudzina, Timothy W. Gant, Marcel Leist, Bennard van Ravenzwaay
Publié dans: Archives of Toxicology, Numéro 96, 2022, Page(s) 743–766, ISSN 0340-5761
Éditeur: Springer Verlag
DOI: 10.1007/s00204-021-03215-9

Direct recording of action potentials of cardiomyocytes through solution processed planar electrolyte-gated field-effect transistors

Auteurs: Adrica Kyndiah, Michele Dipalo, Alireza Molazemhosseini, Fabrizio Antonio Viola, Francesco Modena, Giuseppina Iachetta, Nicolas F. Zorn, Felix J. Berger, Jana Zaumseil, Mario Caironi, Francesco De Angelis
Publié dans: Sensors and Actuators B: Chemical, Numéro Volume 393, 134227, 2023, ISSN 0925-4005
Éditeur: Elsevier BV
DOI: 10.1016/j.snb.2023.134227

Programmed neurite degeneration in human central nervous system neurons driven by changes in NAD+ metabolism

Auteurs: Markus Brüll, Selina Multrus, Michael Schäfer, Ivana Celardo, Christiaan Karreman, Marcel Leist
Publié dans: Cell Death & Disease, Numéro 16, 2025, ISSN 2041-4889
Éditeur: Nature Publishing Group
DOI: 10.1038/s41419-024-07326-w

Passive Recording of Bioelectrical Signals from Non-Excitable Cells by Fluorescent Mirroring

Auteurs: Rosalia Moreddu, Alessio Boschi, Marta d’Amora, Aliaksandr Hubarevich, Michele Dipalo*, and Francesco De Angelis*
Publié dans: Nano Letters, Numéro 2023, 23, 8, 2023, Page(s) 3217–3223, ISSN 1530-6984
Éditeur: American Chemical Society
DOI: 10.1021/acs.nanolett.2c05053

Inhibition of Neural Crest Cell Migration by Strobilurin Fungicides and Other Mitochondrial Toxicants

Auteurs: Viktoria Magel, Jonathan Blum, Xenia Dolde, Heidrun Leisner, Karin Grillberger, Hiba Khalidi, Iain Gardner, Gerhard F. Ecker, Giorgia Pallocca, Nadine Dreser, Marcel Leist
Publié dans: Cells, Numéro 13, 2024, Page(s) 2057, ISSN 2073-4409
Éditeur: MDPI
DOI: 10.3390/cells13242057

Long-term in vitro recording of cardiac action potentials on microelectrode arrays for chronic cardiotoxicity assessment

Auteurs: Giuseppina Iachetta, Giovanni Melle, Nicolò Colistra, Francesco Tantussi, Francesco De Angelis & Michele Dipalo
Publié dans: Archives of Toxicology, Numéro 97, 2023, Page(s) 509–522, ISSN 0340-5761
Éditeur: Springer Verlag
DOI: 10.1007/s00204-022-03422-y

Classification of Developmental Toxicants in a Human iPSC Transcriptomics-Based Test

Auteurs: Anna Cherianidou; Florian Seidel; Franziska Kappenberg; Nadine Dreser; Jonathan Blum; Tanja Waldmann; Nils Blüthgen; Johannes Meisig; Katrin Madjar; Margit Henry; Tamara Rotshteyn; Rosemarie Marchan; Karolina Edlund; Marcel Leist; Jörg Rahnenführer; Agapios Sachinidis; Jan G. Hengstler
Publié dans: Chemical Research in Toxicology, Numéro 35, 5, 2022, Page(s) 760–773, ISSN 0893-228X
Éditeur: American Chemical Society
DOI: 10.1021/acs.chemrestox.1c00392

The long way from raw data to NAM-based information: Overview on data layers and processing steps

Auteurs: Jonathan Blum , Markus Brüll, Jan G. Hengstler, Daniel R. Dietrich, Andreas J. Gruber, Michele Dipalo, Udo Kraushaar, Iris Mangas, Andrea Terron, Ellen Fritsche, Philip Marx-Stoelting, Barry Hardy, Andreas Schepky, Sylvia Escher, Thomas Hartung, Robert Landsiedel, Alex Odermatt, Magdalini Sachana, Katharina Koch, Arif Dönmez, Stefan Masjosthusmann, Kathrin Bothe, Stefan Schildknecht, Mario Beil
Publié dans: ALTEX, Numéro 42, 2025, ISSN 1868-596X
Éditeur: ALTEX Edition
DOI: 10.14573/altex.2412171

Chemical and Biological Mechanisms Relevant to the Rescue of MG-132-Treated Neurons by Cysteine

Auteurs: Anna-Katharina Ückert, Ilinca Suciu, Anja Land, Anna-Sophie Spreng, Hannah Welte, Doreen Herzog, Michael Basler, Marcel Leist
Publié dans: Antioxidants, Numéro 14, 2025, Page(s) 128, ISSN 2076-3921
Éditeur: MDPI AG
DOI: 10.3390/antiox14020128

G × E interactions as a basis for toxicological uncertainty

Auteurs: Ilinca Suciu; David Pamies; Roberta Peruzzo; Petra H. Wirtz; Lena Smirnova; Giorgia Pallocca; Christof Hauck; Mark T. D. Cronin; Jan G. Hengstler; Thomas Brunner; Thomas Hartung; Ivano Amelio; Marcel Leist
Publié dans: Archives of Toxicology, Numéro 97, 2023, Page(s) 2035–2049, ISSN 0340-5761
Éditeur: Springer Verlag
DOI: 10.1007/s00204-023-03500-9

Circulating (Poly)phenol Metabolites: Neuroprotection in a 3D Cell Model of Parkinson's Disease

Auteurs: Carecho, Rafael; Figueira, Inês; Terrasso, Ana Paula; Godinho‐Pereira, Joana; de Oliveira Sequeira, Catarina; Pereira, Sofia Azeredo; Milenkovic, Dragan; Leist, Marcel; Brito, Catarina; Nunes dos Santos, Cláudia
Publié dans: Molecular Nutrition & Food Research, Numéro 2100959, 2022, ISSN 1613-4125
Éditeur: John Wiley & Sons Ltd.
DOI: 10.1002/mnfr.202100959

Profiling of Human Neural Crest Chemoattractant Activity as a Replacement of Fetal Bovine Serum for In Vitro Chemotaxis Assays

Auteurs: Xenia Dolde; Christiaan Karreman; Marianne Wiechers; Stefan Schildknecht; Marcel Leist
Publié dans: International Journal of Molecular Sciences, Vol 22, Iss 10079, p 10079 (2021), Numéro 22(18), 2021, Page(s) 10079, ISSN 1422-0067
Éditeur: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms221810079

Recommendations on fit-for-purpose criteria to establish quality management for microphysiological systems and for monitoring their reproducibility

Auteurs: David Pamies, Jason Ekert, Marie-Gabrielle Zurich, Olivier Frey, Sophie Werner, Monica Piergiovanni, Benjamin S. Freedman, Adrian Kee Keong Teo, Hendrik Erfurth, Darwin R. Reyes, Peter Loskill, Pelin Candarlioglu, Laura Suter-Dick, Shan Wang, Thomas Hartung, Sandra Coecke, Glyn N. Stacey, Beren Atac Wagegg, Eva-Maria Dehne, Francesca Pistollato, Marcel Leist
Publié dans: Stem Cell Reports, Numéro 19, 2024, Page(s) 604-617, ISSN 2213-6711
Éditeur: Cell Press
DOI: 10.1016/j.stemcr.2024.03.009

Acute effects of the imidacloprid metabolite desnitro-imidacloprid on human nACh receptors relevant for neuronal signaling.

Auteurs: Dominik Loser; Dominik Loser; Karin Grillberger; Maria G. Hinojosa; Jonathan Blum; Yves Haufe; Timm Danker; Ylva Johansson; Clemens Möller; Annette Nicke; Susanne Hougaard Bennekou; Iain Gardner; Caroline Bauch; Paul Walker; Anna Forsby; Gerhard F. Ecker; Udo Kraushaar; Marcel Leist
Publié dans: Archives of Toxicology, Numéro 95, 2021, Page(s) 3695–3716, ISSN 0340-5761
Éditeur: Springer Verlag
DOI: 10.1007/s00204-021-03168-z

Longitudinal and Noninvasive Intracellular Recordings of Spontaneous Electrophysiological Activity in Rat Primary Neurons on Planar MEA Electrodes

Auteurs: Rustamzhon Melikov, Giuseppina Iachetta, Marta d'Amora, Giovanni Melle, Silvia Conti, Francesco Tantussi, Michele Dipalo, Francesco De Angelis
Publié dans: Advanced Materials, Numéro 2412697, 2025, ISSN 0935-9648
Éditeur: United Nations Industrial Developement Organization
DOI: 10.1002/adma.202412697