TOXicity assessment on neurons and cardiomyocytes by means of FluoRescence Emitting Electrodes

Informacje na temat projektu

TOX-Free

Identyfikator umowy o grant: 964518

DOI

10.3030/964518

Projekt został zamknięty

Data podpisania przez KE 16 Grudnia 2020

Data rozpoczęcia 1 Czerwca 2021

Data zakończenia 31 Maja 2025

Finansowanie w ramach

• EXCELLENT SCIENCE - Future and Emerging Technologies (FET)

Koszt całkowity

€ 3 035 166,25

Wkład UE

€ 3 035 166,25

Koordynowany przez

FONDAZIONE ISTITUTO ITALIANO DI TECNOLOGIA
Italy

CORDIS oferuje możliwość skorzystania z odnośników do publicznie dostępnych publikacji i rezultatów projektów realizowanych w ramach programów ramowych HORYZONT.

Odnośniki do rezultatów i publikacji związanych z poszczególnymi projektami 7PR, a także odnośniki do niektórych konkretnych kategorii wyników, takich jak zbiory danych i oprogramowanie, są dynamicznie pobierane z systemu OpenAIRE .

Rezultaty

Websites, patent fillings, videos etc. (1)

Project website and logo

Documents, reports (1)

Report on the readout capabilities of VICE 1.0 with neurons

The report will include experimental data on the readout capabilities of VICE 10 with neuronal cultures

Publikacje

Peer reviewed articles (39)

Label-Free Detection of Biochemical Changes during Cortical Organoid Maturation via Raman Spectroscopy and Machine Learning

Autorzy: Giulia Bruno, Michal Lipinski, Koseki J. Kobayashi-Kirschvink, Christian Tentellino, Peter T. C. So, Jeon Woong Kang, Francesco De Angelis
Opublikowane w: Analytical Chemistry, Numer 97, 2025, Strona(/y) 5029-5037, ISSN 0003-2700
Wydawca: American Chemical Society
DOI: 10.1021/acs.analchem.4c05661

On the usefulness of animals as a model system (part II): Considering benefits within distinct use domains

Autorzy: Pallocca, Giorgia; Leist, Marcel
Opublikowane w: ALTEX- Alternatives to Animal Experimentation, 2022, ISSN 1868-8551
Wydawca: ALTEX Edition
DOI: 10.14573/altex.2207111

New approach methods to assess developmental and adult neurotoxicity for regulatory use: a PARC work package 5 project

Autorzy: Tamara Tal, Oddvar Myhre, Ellen Fritsche, Joëlle Rüegg, Kai Craenen, Kiara Aiello-Holden, Caroline Agrillo, Patrick J. Babin, Beate I. Escher, Hubert Dirven, Kati Hellsten, Kristine Dolva, Ellen Hessel, Harm J. Heusinkveld, Yavor Hadzhiev, Selma Hurem, Karolina Jagiello, Beata Judzinska, Nils Klüver, Anja Knoll-Gellida, Britta A. Kühne, Marcel Leist, Malene Lislien, Jan L. Lyche, Ferenc Mülle
Opublikowane w: Frontiers in Toxicology, Numer 6, 2024, ISSN 2673-3080
Wydawca: Frontiers
DOI: 10.3389/ftox.2024.1359507

Dynamic Metabolic and Transcriptional Responses of Proteasome-Inhibited Neurons

Autorzy: Ilinca Suciu; Johannes Delp; Simon Gutbier; Anna-Katharina Ückert; Anna-Sophie Spreng; Philipp Eberhard; Christiaan Karreman; Falk Schreiber; Katrin Madjar; Jörg Rahnenführer; Ivana Celardo; Ivano Amelio; Marcel Leist
Opublikowane w: Antioxidants, 2023, ISSN 2076-3921
Wydawca: MDPI
DOI: 10.3390/antiox12010164

Leveraging biomarkers and translational medicine for preclinical safety - Lessons for advancing the validation of alternatives to animal testing

Autorzy: Thomas Hartung , Nicholas M. P. King, Nicole Kleinstreuer, Marcel Leist, Danilo A. Tagle
Opublikowane w: ALTEX, Numer 41, 2024, ISSN 1868-596X
Wydawca: ALTEX Edition
DOI: 10.14573/altex.2410011

Revolutionizing developmental neurotoxicity testing – a journey from animal models to advanced in vitro systems

Autorzy: Lena Smirnova, Helena T. Hogberg, Marcel Leist, Thomas Hartung
Opublikowane w: ALTEX, Numer Vol. 41 No. 2, 2024, Strona(/y) 152–178, ISSN 1868-596X
Wydawca: ALTEX Edition
DOI: 10.14573/altex.2403281

High Accuracy Classification of Developmental Toxicants by In Vitro Tests of Human Neuroepithelial and Cardiomyoblast Differentiation.

Autorzy: Florian Seidel; Anna Cherianidou; Franziska Kappenberg; Miriam Marta; Nadine Dreser; Jonathan Blum; Tanja Waldmann; Nils Blüthgen; Johannes Meisig; Katrin Madjar; Margit Henry; Tamara Rotshteyn; Andreas Scholtz-Illigens; Rosemarie Marchan; Karolina Edlund; Marcel Leist; Jörg Rahnenführer; Agapios Sachinidis; Jan Georg Hengstler
Opublikowane w: Cells, Numer 11, 2022, Strona(/y) 3404, ISSN 2073-4409
Wydawca: MDPI
DOI: 10.3390/cells11213404

Analysis of health concerns not addressed by REACH for low tonnage chemicals and opportunities for new approach methodology

Autorzy: Philip Botham; Mark T. D. Cronin; Richard Currie; John Doe; Dorothee Funk-Weyer; Timothy W. Gant; Marcel Leist; Sue Marty; Bennard van Ravenzwaay; Carl Westmoreland
Opublikowane w: Archives of Toxicology, 2023, ISSN 0340-5761
Wydawca: Springer Verlag
DOI: 10.1007/s00204-023-03601-5

Distinct and Dynamic Transcriptome Adaptations of iPSC-Generated Astrocytes after Cytokine Stimulation

Autorzy: Anna-Sophie Spreng; Markus Brüll; Heidrun Leisner; Ilinca Suciu; Marcel Leist
Opublikowane w: Cells, Numer Volume 11; Numer 17; Pages: 2644, 2022, ISSN 2073-4409
Wydawca: MDPI
DOI: 10.3390/cells11172644

Electrode- and Label-Free Assessment of Electrophysiological Firing Rates through Cytochrome C Monitoring via Raman Spectroscopy

Autorzy: Christian Tentellino, Marta d’Amora, Rustamzhon Melikov, Giuseppina Iachetta, Giulia Bruno, Francesco Tantussi, Michele Dipalo, Francesco De Angelis
Opublikowane w: ACS Sensors, Numer 10, 2025, Strona(/y) 1228-1236, ISSN 2379-3694
Wydawca: American Chemical Society (ACS)
DOI: 10.1021/acssensors.4c03133

Assay for evaluation of proarrhythmic effects of herbal products: Case study with 12 Evodia preparations

Autorzy: Bozhidar Baltov; Stanislav Beyl; Igor Baburin; Jakob Reinhardt; Phillip Szkokan; Aleksandra Garifulina; Eugen Timin; Udo Kraushaar; Olivier Potterat; Matthias Hamburger; Philipp Kügler; Steffen Hering
Opublikowane w: Toxicology Reports, Numer 2, 2023, ISSN 2214-7500
Wydawca: Elsevier Scientific Publishers Ireland
DOI: 10.1016/j.toxrep.2023.04.014

Modeling ferroptosis in human dopaminergic neurons: Pitfalls and opportunities for neurodegeneration research

Autorzy: Nadine Renner, Franziska Schöb, Regina Pape, Ilinca Suciu, Anna-Sophie Spreng, Anna-Katharina Ückert, Eike Cöllen, Federica Bovio, Bruno Chilian, Johannes Bauer, Stefan Röpcke, Jörg Bergemann, Marcel Leist, Stefan Schildknecht
Opublikowane w: Redox Biology, Numer 73, 2024, Strona(/y) 103165, ISSN 2213-2317
Wydawca: Elsevier BV
DOI: 10.1016/j.redox.2024.103165

Synuclein Family Members Prevent Membrane Damage by Counteracting α-Synuclein Aggregation.

Autorzy: Christian Scheibe; Christiaan Karreman; Stefan Schildknecht; Marcel Leist; Karin Hauser
Opublikowane w: biomolecules, Numer Numer 8, 2021, Strona(/y) 1067, ISSN 2218-273X
Wydawca: mdpi
DOI: 10.3390/biom11081067

Laser-Induced Action Potential-Like Measurements of Cardiomyocytes on Microelectrode Arrays for Increased Predictivity of Safety Pharmacology

Autorzy: Udo Kraushaar; Karin Gebhardt; Timm Danker; Jasmin Schaefer
Opublikowane w: JoVE, 2022, ISSN 1940-087X
Wydawca: MYJoVE Corporation
DOI: 10.3791/64355

Optimization of the TeraTox assay for preclinical teratogenicity assessment

Autorzy: Manuela Jaklin; Jitao David Zhang; Nicole Schaefer; Nicole Clemann; Paul Barrow; Erich Kueng; Lisa Sach-Peltason; Claudia McGinnis; Marcel Leist; Stefan Kustermann
Opublikowane w: Toxicological Sciences, Numer Volume 188, Numer 1, 2022, Strona(/y) 17–33, ISSN 1096-6080
Wydawca: Oxford University Press
DOI: 10.1101/2021.07.06.451364

Preparation of Viable Human Neurites for Neurobiological and Neurodegeneration Studies

Autorzy: Markus Brüll, Nils Geese, Ivana Celardo, Michael Laumann, Marcel Leist
Opublikowane w: Cells, Numer 13, 2025, Strona(/y) 242, ISSN 2073-4409
Wydawca: MDPI
DOI: 10.3390/cells13030242

Generation of Human Nociceptor-Enriched Sensory Neurons for the Study of Pain-Related Dysfunctions

Autorzy: Anna-Katharina Holzer; Christiaan Karreman; Ilinca Suciu; Lara-Seline Furmanowsky; Harald Wohlfarth; Dominik Loser; Wilhelm G Dirks; Emilio Pardo González; Marcel Leist
Opublikowane w: Stem Cells Transl Med., Numer Volume 11, Numer 7, 2022, Strona(/y) 727–741, ISSN 2157-6564
Wydawca: AlphaMed Press
DOI: 10.1101/2022.02.19.480828

Probing ND7/23 neuronal cells before and after differentiation with SERS using Sharp-tipped Au nanopyramid arrays

Autorzy: Marzia Iarossi, Aliaksandr Hubarevich, Giuseppina Iachetta, MicheleDipalo, Jian-An Huang, Daniel Darvill, Francesco De Angelis
Opublikowane w: Sensors and Actuators B: Chemical, Numer Volume 361, 2022, Strona(/y) 131724, ISSN 0925-4005
Wydawca: Elsevier BV
DOI: 10.1016/j.snb.2022.131724

Definition of the Neurotoxicity-Associated Metabolic Signature Triggered by Berberine and Other Respiratory Chain Inhibitors

Autorzy: Ilinca Suciu, Johannes Delp, Simon Gutbier, Julian Suess, Lars Henschke, Ivana Celardo, Thomas U. Mayer, Ivano Amelio, Marcel Leist
Opublikowane w: Antioxidants, Numer 13, 2025, Strona(/y) 49, ISSN 2076-3921
Wydawca: MDPI
DOI: 10.3390/antiox13010049

Establishment of a Human Cell-Based in Vitro Battery to Assess Developmental Neurotoxicity Hazard of Chemicals

Autorzy: Jonathan Blum; Stefan Masjosthusmann; Kristina Bartmann; Farina Bendt; Xenia Dolde; Arif Dönmez; Nils Förster; Anna-Katharina Holzer; Ulrike Hübenthal; Hagen Eike Keßel; Sadiye Kilic; Jördis Klose; Melanie Pahl; Lynn-Christin Stürzl; Iris Mangas; Andrea Terron; Kevin M. Crofton; Martin Scholze; Axel Mosig; Marcel Leist; Ellen Fritsche
Opublikowane w: Chemosphere, 2023, ISSN 0045-6535
Wydawca: Pergamon Press Ltd.
DOI: 10.2139/ssrn.4161670

Specific Attenuation of Purinergic Signaling during Bortezomib-Induced Peripheral Neuropathy In Vitro

Autorzy: Anna-Katharina Holzer; Ilinca Suciu; Christiaan Karreman; Thomas Goj; Marcel Leist
Opublikowane w: International Journal of Molecular Sciences, 2022, ISSN 1422-0067
Wydawca: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms23073734

Elements and development processes for test methods in toxicology and human health-relevant life science research

Autorzy: Cöllen, Eike; Tanaskov, Yaroslav; Holzer, Anna-Katharina; Dipalo, Michele; Schäfer, Jasmin; Kraushaar, Udo; Leist, Marcel
Opublikowane w: ALTEX, Numer 8, 2024, ISSN 1868-596X
Wydawca: Altex Edition

On the usefulness of animals as a model system (part I): Overview of criteria and focus on robustness

Autorzy: Pallocca, Giorgia; Rovida, Costanza; Leist, Marcel
Opublikowane w: Altex, 2022, ISSN 1868-8551
Wydawca: ALTEX Edition
DOI: 10.14573/altex.2203291

Identification of the bacterial metabolite aerugine as potential trigger of human dopaminergic neurodegeneration

Autorzy: Anna-Katharina Ückert, Sina Rütschlin, Simon Gutbier, Nathalie Christine Wörz, Mahfuzur R. Miah, Airton C. Martins, Isa Hauer, Anna-Katharina Holzer, Birthe Meyburg, Ann-Kathrin Mix, Christof Hauck, Michael Aschner, Thomas Böttcher, Marcel Leist
Opublikowane w: Environment International, Numer 180, 2023, Strona(/y) 108229, ISSN 0160-4120
Wydawca: Pergamon Press Ltd.
DOI: 10.1016/j.envint.2023.108229

A framework for chemical safety assessment incorporating new approach methodologies within REACH

Autorzy: Nicholas Ball, Remi Bars, Philip A. Botham, Andreea Cuciureanu, Mark T. D. Cronin, John E. Doe, Tatsiana Dudzina, Timothy W. Gant, Marcel Leist, Bennard van Ravenzwaay
Opublikowane w: Archives of Toxicology, Numer 96, 2022, Strona(/y) 743–766, ISSN 0340-5761
Wydawca: Springer Verlag
DOI: 10.1007/s00204-021-03215-9

Direct recording of action potentials of cardiomyocytes through solution processed planar electrolyte-gated field-effect transistors

Autorzy: Adrica Kyndiah, Michele Dipalo, Alireza Molazemhosseini, Fabrizio Antonio Viola, Francesco Modena, Giuseppina Iachetta, Nicolas F. Zorn, Felix J. Berger, Jana Zaumseil, Mario Caironi, Francesco De Angelis
Opublikowane w: Sensors and Actuators B: Chemical, Numer Volume 393, 134227, 2023, ISSN 0925-4005
Wydawca: Elsevier BV
DOI: 10.1016/j.snb.2023.134227

Programmed neurite degeneration in human central nervous system neurons driven by changes in NAD+ metabolism

Autorzy: Markus Brüll, Selina Multrus, Michael Schäfer, Ivana Celardo, Christiaan Karreman, Marcel Leist
Opublikowane w: Cell Death & Disease, Numer 16, 2025, ISSN 2041-4889
Wydawca: Nature Publishing Group
DOI: 10.1038/s41419-024-07326-w

Passive Recording of Bioelectrical Signals from Non-Excitable Cells by Fluorescent Mirroring

Autorzy: Rosalia Moreddu, Alessio Boschi, Marta d’Amora, Aliaksandr Hubarevich, Michele Dipalo*, and Francesco De Angelis*
Opublikowane w: Nano Letters, Numer 2023, 23, 8, 2023, Strona(/y) 3217–3223, ISSN 1530-6984
Wydawca: American Chemical Society
DOI: 10.1021/acs.nanolett.2c05053

Inhibition of Neural Crest Cell Migration by Strobilurin Fungicides and Other Mitochondrial Toxicants

Autorzy: Viktoria Magel, Jonathan Blum, Xenia Dolde, Heidrun Leisner, Karin Grillberger, Hiba Khalidi, Iain Gardner, Gerhard F. Ecker, Giorgia Pallocca, Nadine Dreser, Marcel Leist
Opublikowane w: Cells, Numer 13, 2024, Strona(/y) 2057, ISSN 2073-4409
Wydawca: MDPI
DOI: 10.3390/cells13242057

Long-term in vitro recording of cardiac action potentials on microelectrode arrays for chronic cardiotoxicity assessment

Autorzy: Giuseppina Iachetta, Giovanni Melle, Nicolò Colistra, Francesco Tantussi, Francesco De Angelis & Michele Dipalo
Opublikowane w: Archives of Toxicology, Numer 97, 2023, Strona(/y) 509–522, ISSN 0340-5761
Wydawca: Springer Verlag
DOI: 10.1007/s00204-022-03422-y

Classification of Developmental Toxicants in a Human iPSC Transcriptomics-Based Test

Autorzy: Anna Cherianidou; Florian Seidel; Franziska Kappenberg; Nadine Dreser; Jonathan Blum; Tanja Waldmann; Nils Blüthgen; Johannes Meisig; Katrin Madjar; Margit Henry; Tamara Rotshteyn; Rosemarie Marchan; Karolina Edlund; Marcel Leist; Jörg Rahnenführer; Agapios Sachinidis; Jan G. Hengstler
Opublikowane w: Chemical Research in Toxicology, Numer 35, 5, 2022, Strona(/y) 760–773, ISSN 0893-228X
Wydawca: American Chemical Society
DOI: 10.1021/acs.chemrestox.1c00392

The long way from raw data to NAM-based information: Overview on data layers and processing steps

Autorzy: Jonathan Blum , Markus Brüll, Jan G. Hengstler, Daniel R. Dietrich, Andreas J. Gruber, Michele Dipalo, Udo Kraushaar, Iris Mangas, Andrea Terron, Ellen Fritsche, Philip Marx-Stoelting, Barry Hardy, Andreas Schepky, Sylvia Escher, Thomas Hartung, Robert Landsiedel, Alex Odermatt, Magdalini Sachana, Katharina Koch, Arif Dönmez, Stefan Masjosthusmann, Kathrin Bothe, Stefan Schildknecht, Mario Beil
Opublikowane w: ALTEX, Numer 42, 2025, ISSN 1868-596X
Wydawca: ALTEX Edition
DOI: 10.14573/altex.2412171

Chemical and Biological Mechanisms Relevant to the Rescue of MG-132-Treated Neurons by Cysteine

Autorzy: Anna-Katharina Ückert, Ilinca Suciu, Anja Land, Anna-Sophie Spreng, Hannah Welte, Doreen Herzog, Michael Basler, Marcel Leist
Opublikowane w: Antioxidants, Numer 14, 2025, Strona(/y) 128, ISSN 2076-3921
Wydawca: MDPI AG
DOI: 10.3390/antiox14020128

G × E interactions as a basis for toxicological uncertainty

Autorzy: Ilinca Suciu; David Pamies; Roberta Peruzzo; Petra H. Wirtz; Lena Smirnova; Giorgia Pallocca; Christof Hauck; Mark T. D. Cronin; Jan G. Hengstler; Thomas Brunner; Thomas Hartung; Ivano Amelio; Marcel Leist
Opublikowane w: Archives of Toxicology, Numer 97, 2023, Strona(/y) 2035–2049, ISSN 0340-5761
Wydawca: Springer Verlag
DOI: 10.1007/s00204-023-03500-9

Circulating (Poly)phenol Metabolites: Neuroprotection in a 3D Cell Model of Parkinson's Disease

Autorzy: Carecho, Rafael; Figueira, Inês; Terrasso, Ana Paula; Godinho‐Pereira, Joana; de Oliveira Sequeira, Catarina; Pereira, Sofia Azeredo; Milenkovic, Dragan; Leist, Marcel; Brito, Catarina; Nunes dos Santos, Cláudia
Opublikowane w: Molecular Nutrition & Food Research, Numer 2100959, 2022, ISSN 1613-4125
Wydawca: John Wiley & Sons Ltd.
DOI: 10.1002/mnfr.202100959

Profiling of Human Neural Crest Chemoattractant Activity as a Replacement of Fetal Bovine Serum for In Vitro Chemotaxis Assays

Autorzy: Xenia Dolde; Christiaan Karreman; Marianne Wiechers; Stefan Schildknecht; Marcel Leist
Opublikowane w: International Journal of Molecular Sciences, Vol 22, Iss 10079, p 10079 (2021), Numer 22(18), 2021, Strona(/y) 10079, ISSN 1422-0067
Wydawca: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms221810079

Recommendations on fit-for-purpose criteria to establish quality management for microphysiological systems and for monitoring their reproducibility

Autorzy: David Pamies, Jason Ekert, Marie-Gabrielle Zurich, Olivier Frey, Sophie Werner, Monica Piergiovanni, Benjamin S. Freedman, Adrian Kee Keong Teo, Hendrik Erfurth, Darwin R. Reyes, Peter Loskill, Pelin Candarlioglu, Laura Suter-Dick, Shan Wang, Thomas Hartung, Sandra Coecke, Glyn N. Stacey, Beren Atac Wagegg, Eva-Maria Dehne, Francesca Pistollato, Marcel Leist
Opublikowane w: Stem Cell Reports, Numer 19, 2024, Strona(/y) 604-617, ISSN 2213-6711
Wydawca: Cell Press
DOI: 10.1016/j.stemcr.2024.03.009

Acute effects of the imidacloprid metabolite desnitro-imidacloprid on human nACh receptors relevant for neuronal signaling.

Autorzy: Dominik Loser; Dominik Loser; Karin Grillberger; Maria G. Hinojosa; Jonathan Blum; Yves Haufe; Timm Danker; Ylva Johansson; Clemens Möller; Annette Nicke; Susanne Hougaard Bennekou; Iain Gardner; Caroline Bauch; Paul Walker; Anna Forsby; Gerhard F. Ecker; Udo Kraushaar; Marcel Leist
Opublikowane w: Archives of Toxicology, Numer 95, 2021, Strona(/y) 3695–3716, ISSN 0340-5761
Wydawca: Springer Verlag
DOI: 10.1007/s00204-021-03168-z

Longitudinal and Noninvasive Intracellular Recordings of Spontaneous Electrophysiological Activity in Rat Primary Neurons on Planar MEA Electrodes

Autorzy: Rustamzhon Melikov, Giuseppina Iachetta, Marta d'Amora, Giovanni Melle, Silvia Conti, Francesco Tantussi, Michele Dipalo, Francesco De Angelis
Opublikowane w: Advanced Materials, Numer 2412697, 2025, ISSN 0935-9648
Wydawca: United Nations Industrial Developement Organization
DOI: 10.1002/adma.202412697