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CORDIS - EU research results
CORDIS

Biocatalytic upgrading of natural biopolymers for reassembly as multipurpose materials

CORDIS provides links to public deliverables and publications of HORIZON projects.

Links to deliverables and publications from FP7 projects, as well as links to some specific result types such as dataset and software, are dynamically retrieved from OpenAIRE .

Deliverables

Application-driven functional screens for carbohydrate oxidoreductases and transaminases with preferred regioselectivity to promote crosslinking and tailored surface charge (opens in new window)

Establish analytical methods to screen enzyme function including methods based on fluorometry mass spectrometry NMR and zeta potential measurements

Microbial expansin-related proteins with enhanced action on cellulose and chitin (opens in new window)

Incorporate experimental data and molecular modeling to design and then construct microbial expansin related proteins with increased potential to bind and alter the architecture of cellulose andor chitin fibre

Computational framework to identify biophysical signatures for surface acting proteins (opens in new window)

Application and integration of ancestral sequence resurrection molecular modeling techniques and data mining methods for the selection and design of surface acting proteins

Workshop on inclusivity and work-life balance for the PIs and researchers (opens in new window)

Leverage existing resources at beneficiary institutions to ensure all project participants complete at least one workshop on inclusivity which will help to ensure retention of underrepresented groups in STEM careers post graduation

Application-driven functional screens for microbial expansin action on bio-fibers (opens in new window)

Establish biophysical methods to screen protein functions including methods based on viscoelasticity porosity and crystallinity eg dynamic light scattering solute exclusion and Xray diffraction

Matrix of compositional, structural and biophysical properties of bio-fibre starting materials (opens in new window)

Evaluate the molecular composition structure and network organisation of the prioritized biofibers using complementary biochemical and biophysical techniques These include glycomic analyses of the structural polysaccharides highresolution low temperature scanning electron microscopy cryoSEM atomic force microscopy solidstate spectroscopy FTIR and ssNMR and small angle scattering techniques SAXS and SANS12

BioUPGRADE Public website created (opens in new window)

Set up a public website to disseminate announcements eg recruitment conferences scientific publications and outreach activities

Publications

Enzymatic upgrading of nanochitin using an ancient lytic polysaccharide monooxygenase (opens in new window)

Author(s): Leire Barandiaran, Borja Alonso-Lerma, Antonio Reifs, Izaskun Larraza, Raquel Olmos-Juste, Alba Fernandez-Calvo, Ylenia Jabalera, Arantxa Eceiza, Raul Perez-Jimenez
Published in: Communications Materials, Issue 3, 2022, ISSN 2662-4443
Publisher: Springer Nature
DOI: 10.1038/s43246-022-00277-9

Sequential extraction of hemicelluloses by subcritical water improves saccharification of hybrid aspen wood grown in greenhouse and field conditions (opens in new window)

Author(s): Pramod Sivan, Emilia Heinonen, Madhavi Latha Gandla, Amparo Jiménez-Quero, Hüsamettin Deniz Özeren, Leif J. Jönsson, Ewa J. Mellerowicz, Francisco Vilaplana
Published in: Green Chemistry, Issue 14/25, 2023, Page(s) 5634-5646, ISSN 1757-7039
Publisher: Royal Society of Chemistry
DOI: 10.1039/d3gc01020a

Compliant mechanical response of the ultrafast folding protein EnHD under force (opens in new window)

Author(s): Antonio Reifs, Irene Ruiz Ortiz, Amaia Ochandorena Saa, Jörg Schönfelder, David De Sancho, Victor Muñoz, Raul Perez-Jimenez
Published in: Communications Physics, Issue 6, 2023, ISSN 2399-3650
Publisher: Springer Nature
DOI: 10.1038/s42005-022-01125-5

Evolution of CRISPR-associated endonucleases as inferred from resurrected proteins (opens in new window)

Author(s): Borja Alonso-Lerma, Ylenia Jabalera, Sara Samperio, Matias Morin, Almudena Fernandez, Logan T. Hille, Rachel A. Silverstein, Ane Quesada-Ganuza, Antonio Reifs, Sergio Fernández-Peñalver, Yolanda Benitez, Lucia Soletto, Jose A. Gavira, Adrian Diaz, Wim Vranken, Avencia Sanchez-Mejias, Marc Güell, Francisco J. M. Mojica, Benjamin P. Kleinstiver, Miguel A. Moreno-Pelayo, Lluis Montoliu, Raul Perez
Published in: Nature Microbiology, Issue 8/1, 2023, Page(s) 77–90, ISSN 2058-5276
Publisher: Nature Publishing Group
DOI: 10.1038/s41564-022-01265-y

Loosenin-Like Proteins from Phanerochaete carnosa Impact Both Cellulose and Chitin Fiber Networks (opens in new window)

Author(s): Mareike Monschein, Eleni Ioannou, Taru Koitto, Leamon A. K. M. Al Amin, Jutta J. Varis, Edward R. Wagner, Kirsi S. Mikkonen, Daniel J. Cosgrove, Emma R. Master
Published in: Applied and Environmental Microbiology, Issue 89/1, 2023, ISSN 0099-2240
Publisher: American Society for Microbiology
DOI: 10.1128/aem.01863-22

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