Biocatalytic upgrading of natural biopolymers for reassembly as multipurpose materials

Información del proyecto

BioUPGRADE

Identificador del acuerdo de subvención: 964764

DOI

10.3030/964764

Proyecto cerrado

Fecha de la firma de la CE 19 Enero 2021

Fecha de inicio 1 Mayo 2021

Fecha de finalización 31 Octubre 2025

Financiado con arreglo a

• EXCELLENT SCIENCE - Future and Emerging Technologies (FET)

Coste total

€ 2 992 140,00

Aportación de la UE

€ 2 992 140,00

Coordinado por

AALTO KORKEAKOULUSAATIO SR
Finland

CORDIS proporciona enlaces a los documentos públicos y las publicaciones de los proyectos de los programas marco HORIZONTE.

Los enlaces a los documentos y las publicaciones de los proyectos del Séptimo Programa Marco, así como los enlaces a algunos tipos de resultados específicos, como conjuntos de datos y «software», se obtienen dinámicamente de OpenAIRE .

Resultado final

Documents, reports (6)

Application-driven functional screens for carbohydrate oxidoreductases and transaminases with preferred regioselectivity to promote crosslinking and tailored surface charge

Establish analytical methods to screen enzyme function including methods based on fluorometry mass spectrometry NMR and zeta potential measurements

Microbial expansin-related proteins with enhanced action on cellulose and chitin

Incorporate experimental data and molecular modeling to design and then construct microbial expansin related proteins with increased potential to bind and alter the architecture of cellulose andor chitin fibre

Computational framework to identify biophysical signatures for surface acting proteins

Application and integration of ancestral sequence resurrection molecular modeling techniques and data mining methods for the selection and design of surface acting proteins

Workshop on inclusivity and work-life balance for the PIs and researchers

Leverage existing resources at beneficiary institutions to ensure all project participants complete at least one workshop on inclusivity which will help to ensure retention of underrepresented groups in STEM careers post graduation

Application-driven functional screens for microbial expansin action on bio-fibers

Establish biophysical methods to screen protein functions including methods based on viscoelasticity porosity and crystallinity eg dynamic light scattering solute exclusion and Xray diffraction

Matrix of compositional, structural and biophysical properties of bio-fibre starting materials

Evaluate the molecular composition structure and network organisation of the prioritized biofibers using complementary biochemical and biophysical techniques These include glycomic analyses of the structural polysaccharides highresolution low temperature scanning electron microscopy cryoSEM atomic force microscopy solidstate spectroscopy FTIR and ssNMR and small angle scattering techniques SAXS and SANS12

Websites, patent fillings, videos etc. (1)

BioUPGRADE Public website created

Set up a public website to disseminate announcements eg recruitment conferences scientific publications and outreach activities

Publicaciones

Peer reviewed articles (14)

Comparative assessment of chemical and biochemical approaches for the activation of lignocellulosic materials and emerging opportunities for expansin-related proteins

Autores: Salla Hiltunen, Janak Sapkota, Eleni Ioannou, Majid Haddad Momeni, Emma Master, Matti Ristolainen
Publicado en: Cellulose, Edición 31, 2024, Página(s) 147-168, ISSN 0969-0239
Editor: Blackie Academic & Professional
DOI: 10.1007/s10570-023-05637-3

Enzymatic Routes to Designer Hemicelluloses for Use in Biobased Materials

Autores: Thu V. Vuong, Mohammad Aghajohari, Xuebin Feng, Amanda K. Woodstock, Deepti M. Nambiar, Zeina C. Sleiman, Breeanna R. Urbanowicz, Emma R. Master
Publicado en: JACS Au, Edición 4, 2024, Página(s) 4044-4065, ISSN 2691-3704
Editor: American Chemical Society (ACS)
DOI: 10.1021/jacsau.4c00469

Carbohydrate-binding domain CBM63 of microbial expansin-like BsEXLX1 facilitates the adsorption of expansin-related proteins to hemicelluloses in plant secondary cell walls

Autores: Pramod Sivan, Deepika Dahiya, Ylenia Jabalera, Taru Koitto, Raul Perez-Jimenez, Ewa J. Mellerowicz, Emma Master, Francisco Vilaplana
Publicado en: Biotechnology for Biofuels and Bioproducts, Edición 18, 2025, ISSN 2731-3654
Editor: BioMed Central Ltd.
DOI: 10.1186/s13068-025-02674-x

Insights into the action of phylogenetically diverse microbial expansins on the structure of cellulose microfibrils

Autores: Majid Haddad Momeni, Aleksi Zitting, Vilma Jäämuru, Rosaliina Turunen, Paavo Penttilä, Garry W. Buchko, Salla Hiltunen, Natalia Maiorova, Anu Koivula, Janak Sapkota, Kaisa Marjamaa, Emma R. Master
Publicado en: Biotechnology for Biofuels and Bioproducts, Edición 17, 2024, ISSN 2731-3654
Editor: BioMed Central Ltd.
DOI: 10.1186/s13068-024-02500-w

Enzymatic upgrading of nanochitin using an ancient lytic polysaccharide monooxygenase

Autores: Leire Barandiaran, Borja Alonso-Lerma, Antonio Reifs, Izaskun Larraza, Raquel Olmos-Juste, Alba Fernandez-Calvo, Ylenia Jabalera, Arantxa Eceiza, Raul Perez-Jimenez
Publicado en: Communications Materials, Edición 3, 2022, ISSN 2662-4443
Editor: Springer Nature
DOI: 10.1038/s43246-022-00277-9

Fungal loosenin-like proteins boost the cellulolytic enzyme conversion of pretreated wood fiber and cellulosic pulps

Autores: Deepika Dahiya, Taru Koitto, Kim Kutvonen, Yan Wang, Majid Haddad Momeni, Siiri de Ruijter, Emma R. Master
Publicado en: Bioresource Technology, Edición 394, 2025, Página(s) 130188, ISSN 0960-8524
Editor: Elsevier BV
DOI: 10.1016/j.biortech.2023.130188

Sequential extraction of hemicelluloses by subcritical water improves saccharification of hybrid aspen wood grown in greenhouse and field conditions

Autores: Pramod Sivan, Emilia Heinonen, Madhavi Latha Gandla, Amparo Jiménez-Quero, Hüsamettin Deniz Özeren, Leif J. Jönsson, Ewa J. Mellerowicz, Francisco Vilaplana
Publicado en: Green Chemistry, Edición 14/25, 2023, Página(s) 5634-5646, ISSN 1757-7039
Editor: Royal Society of Chemistry
DOI: 10.1039/d3gc01020a

Uncovering Sequence and Structural Characteristics of Fungal Expansin‐Related Proteins With Potential to Drive Substrate Targeting

Autores: Anna Pohto, Taru Koitto, Deepika Dahiya, Alessandra Castro, Elizaveta Sidorova, Martina Huusela, Scott E. Baker, Adrian Tsang, Emma Master
Publicado en: Proteins: Structure, Function, and Bioinformatics, 2025, ISSN 0887-3585
Editor: Wiley-Liss Inc
DOI: 10.1002/prot.70029

SANS investigation of fungal loosenins reveals substrate-dependent impacts of protein action on the inter-microfibril arrangement of cellulosic substrates

Autores: Deepika Dahiya, Zsuzsanna Péter-Szabó, Manjula Senanayake, Sai Venkatesh Pingali, Wellington C. Leite, James Byrnes, Garry W. Buchko, Pramod Sivan, Francisco Vilaplana, Emma R. Master, Hugh O’Neill
Publicado en: Biotechnology for Biofuels and Bioproducts, Edición 18, 2025, ISSN 2731-3654
Editor: BioMed Central Ltd.
DOI: 10.1186/s13068-025-02618-5

Effects of sequential ball milling and loosenin-like protein treatment on pulp fibers

Autores: Salla Hiltunen, Deepika Dahiya, Martina Huusela, Emma Master, Matti Ristolainen, Janak Sapkota
Publicado en: Industrial Crops and Products, Edición 237, 2025, Página(s) 122177, ISSN 0926-6690
Editor: Elsevier BV
DOI: 10.1016/j.indcrop.2025.122177

Compliant mechanical response of the ultrafast folding protein EnHD under force

Autores: Antonio Reifs, Irene Ruiz Ortiz, Amaia Ochandorena Saa, Jörg Schönfelder, David De Sancho, Victor Muñoz, Raul Perez-Jimenez
Publicado en: Communications Physics, Edición 6, 2023, ISSN 2399-3650
Editor: Springer Nature
DOI: 10.1038/s42005-022-01125-5

Evolution of CRISPR-associated endonucleases as inferred from resurrected proteins

Autores: Borja Alonso-Lerma, Ylenia Jabalera, Sara Samperio, Matias Morin, Almudena Fernandez, Logan T. Hille, Rachel A. Silverstein, Ane Quesada-Ganuza, Antonio Reifs, Sergio Fernández-Peñalver, Yolanda Benitez, Lucia Soletto, Jose A. Gavira, Adrian Diaz, Wim Vranken, Avencia Sanchez-Mejias, Marc Güell, Francisco J. M. Mojica, Benjamin P. Kleinstiver, Miguel A. Moreno-Pelayo, Lluis Montoliu, Raul Perez
Publicado en: Nature Microbiology, Edición 8/1, 2023, Página(s) 77–90, ISSN 2058-5276
Editor: Nature Publishing Group
DOI: 10.1038/s41564-022-01265-y

Loosenin-Like Proteins from Phanerochaete carnosa Impact Both Cellulose and Chitin Fiber Networks

Autores: Mareike Monschein, Eleni Ioannou, Taru Koitto, Leamon A. K. M. Al Amin, Jutta J. Varis, Edward R. Wagner, Kirsi S. Mikkonen, Daniel J. Cosgrove, Emma R. Master
Publicado en: Applied and Environmental Microbiology, Edición 89/1, 2023, ISSN 0099-2240
Editor: American Society for Microbiology
DOI: 10.1128/aem.01863-22

Phylogenetically distinct fungal expansins show different binding preferences towards cellulosic materials and enhance cellulase activity

Autores: Taru Koitto, Deepika Dahiya, Martina Huusela, Merja Penttilä, Emma Master
Publicado en: Current Research in Biotechnology, Edición 9, 2025, Página(s) 100296, ISSN 2590-2628
Editor: Elsevier BV
DOI: 10.1016/j.crbiot.2025.100296

Conference proceedings (1)

Microbial Expansin Related Proteins for Improved Enzymatic Action for Conversion of Lignocellulose to Platform Sugars

Autores: Deepika Dahiya, Siiri de Ruijter, Kim Kutvonen, Emma Master, Amy Yan Wang
Publicado en: NWBC 2022: The 10th Nordic Wood Biorefinery Conference, 2022, Página(s) 220-221, ISBN 978-951-38-8772-8
Editor: VTT Technical Research Centre of Finland
DOI: 10.32040/2242-122x.2022.t409