Biocatalytic upgrading of natural biopolymers for reassembly as multipurpose materials

Informations projet

BioUPGRADE

N° de convention de subvention: 964764

DOI

10.3030/964764

Projet clôturé

Date de signature de la CE 19 Janvier 2021

Date de début 1 Mai 2021

Date de fin 31 Octobre 2025

Financé au titre de

• EXCELLENT SCIENCE - Future and Emerging Technologies (FET)

Coût total

€ 2 992 140,00

Contribution de l’UE

€ 2 992 140,00

Coordonné par

AALTO KORKEAKOULUSAATIO SR
Finland

CORDIS fournit des liens vers les livrables publics et les publications des projets HORIZON.

Les liens vers les livrables et les publications des projets du 7e PC, ainsi que les liens vers certains types de résultats spécifiques tels que les jeux de données et les logiciels, sont récupérés dynamiquement sur OpenAIRE .

Livrables

Documents, reports (6)

Application-driven functional screens for carbohydrate oxidoreductases and transaminases with preferred regioselectivity to promote crosslinking and tailored surface charge

Establish analytical methods to screen enzyme function including methods based on fluorometry mass spectrometry NMR and zeta potential measurements

Microbial expansin-related proteins with enhanced action on cellulose and chitin

Incorporate experimental data and molecular modeling to design and then construct microbial expansin related proteins with increased potential to bind and alter the architecture of cellulose andor chitin fibre

Computational framework to identify biophysical signatures for surface acting proteins

Application and integration of ancestral sequence resurrection molecular modeling techniques and data mining methods for the selection and design of surface acting proteins

Workshop on inclusivity and work-life balance for the PIs and researchers

Leverage existing resources at beneficiary institutions to ensure all project participants complete at least one workshop on inclusivity which will help to ensure retention of underrepresented groups in STEM careers post graduation

Application-driven functional screens for microbial expansin action on bio-fibers

Establish biophysical methods to screen protein functions including methods based on viscoelasticity porosity and crystallinity eg dynamic light scattering solute exclusion and Xray diffraction

Matrix of compositional, structural and biophysical properties of bio-fibre starting materials

Evaluate the molecular composition structure and network organisation of the prioritized biofibers using complementary biochemical and biophysical techniques These include glycomic analyses of the structural polysaccharides highresolution low temperature scanning electron microscopy cryoSEM atomic force microscopy solidstate spectroscopy FTIR and ssNMR and small angle scattering techniques SAXS and SANS12

Websites, patent fillings, videos etc. (1)

BioUPGRADE Public website created

Set up a public website to disseminate announcements eg recruitment conferences scientific publications and outreach activities

Publications

Peer reviewed articles (14)

Comparative assessment of chemical and biochemical approaches for the activation of lignocellulosic materials and emerging opportunities for expansin-related proteins

Auteurs: Salla Hiltunen, Janak Sapkota, Eleni Ioannou, Majid Haddad Momeni, Emma Master, Matti Ristolainen
Publié dans: Cellulose, Numéro 31, 2024, Page(s) 147-168, ISSN 0969-0239
Éditeur: Blackie Academic & Professional
DOI: 10.1007/s10570-023-05637-3

Enzymatic Routes to Designer Hemicelluloses for Use in Biobased Materials

Auteurs: Thu V. Vuong, Mohammad Aghajohari, Xuebin Feng, Amanda K. Woodstock, Deepti M. Nambiar, Zeina C. Sleiman, Breeanna R. Urbanowicz, Emma R. Master
Publié dans: JACS Au, Numéro 4, 2024, Page(s) 4044-4065, ISSN 2691-3704
Éditeur: American Chemical Society (ACS)
DOI: 10.1021/jacsau.4c00469

Carbohydrate-binding domain CBM63 of microbial expansin-like BsEXLX1 facilitates the adsorption of expansin-related proteins to hemicelluloses in plant secondary cell walls

Auteurs: Pramod Sivan, Deepika Dahiya, Ylenia Jabalera, Taru Koitto, Raul Perez-Jimenez, Ewa J. Mellerowicz, Emma Master, Francisco Vilaplana
Publié dans: Biotechnology for Biofuels and Bioproducts, Numéro 18, 2025, ISSN 2731-3654
Éditeur: BioMed Central Ltd.
DOI: 10.1186/s13068-025-02674-x

Insights into the action of phylogenetically diverse microbial expansins on the structure of cellulose microfibrils

Auteurs: Majid Haddad Momeni, Aleksi Zitting, Vilma Jäämuru, Rosaliina Turunen, Paavo Penttilä, Garry W. Buchko, Salla Hiltunen, Natalia Maiorova, Anu Koivula, Janak Sapkota, Kaisa Marjamaa, Emma R. Master
Publié dans: Biotechnology for Biofuels and Bioproducts, Numéro 17, 2024, ISSN 2731-3654
Éditeur: BioMed Central Ltd.
DOI: 10.1186/s13068-024-02500-w

Enzymatic upgrading of nanochitin using an ancient lytic polysaccharide monooxygenase

Auteurs: Leire Barandiaran, Borja Alonso-Lerma, Antonio Reifs, Izaskun Larraza, Raquel Olmos-Juste, Alba Fernandez-Calvo, Ylenia Jabalera, Arantxa Eceiza, Raul Perez-Jimenez
Publié dans: Communications Materials, Numéro 3, 2022, ISSN 2662-4443
Éditeur: Springer Nature
DOI: 10.1038/s43246-022-00277-9

Fungal loosenin-like proteins boost the cellulolytic enzyme conversion of pretreated wood fiber and cellulosic pulps

Auteurs: Deepika Dahiya, Taru Koitto, Kim Kutvonen, Yan Wang, Majid Haddad Momeni, Siiri de Ruijter, Emma R. Master
Publié dans: Bioresource Technology, Numéro 394, 2025, Page(s) 130188, ISSN 0960-8524
Éditeur: Elsevier BV
DOI: 10.1016/j.biortech.2023.130188

Sequential extraction of hemicelluloses by subcritical water improves saccharification of hybrid aspen wood grown in greenhouse and field conditions

Auteurs: Pramod Sivan, Emilia Heinonen, Madhavi Latha Gandla, Amparo Jiménez-Quero, Hüsamettin Deniz Özeren, Leif J. Jönsson, Ewa J. Mellerowicz, Francisco Vilaplana
Publié dans: Green Chemistry, Numéro 14/25, 2023, Page(s) 5634-5646, ISSN 1757-7039
Éditeur: Royal Society of Chemistry
DOI: 10.1039/d3gc01020a

Uncovering Sequence and Structural Characteristics of Fungal Expansin‐Related Proteins With Potential to Drive Substrate Targeting

Auteurs: Anna Pohto, Taru Koitto, Deepika Dahiya, Alessandra Castro, Elizaveta Sidorova, Martina Huusela, Scott E. Baker, Adrian Tsang, Emma Master
Publié dans: Proteins: Structure, Function, and Bioinformatics, 2025, ISSN 0887-3585
Éditeur: Wiley-Liss Inc
DOI: 10.1002/prot.70029

SANS investigation of fungal loosenins reveals substrate-dependent impacts of protein action on the inter-microfibril arrangement of cellulosic substrates

Auteurs: Deepika Dahiya, Zsuzsanna Péter-Szabó, Manjula Senanayake, Sai Venkatesh Pingali, Wellington C. Leite, James Byrnes, Garry W. Buchko, Pramod Sivan, Francisco Vilaplana, Emma R. Master, Hugh O’Neill
Publié dans: Biotechnology for Biofuels and Bioproducts, Numéro 18, 2025, ISSN 2731-3654
Éditeur: BioMed Central Ltd.
DOI: 10.1186/s13068-025-02618-5

Effects of sequential ball milling and loosenin-like protein treatment on pulp fibers

Auteurs: Salla Hiltunen, Deepika Dahiya, Martina Huusela, Emma Master, Matti Ristolainen, Janak Sapkota
Publié dans: Industrial Crops and Products, Numéro 237, 2025, Page(s) 122177, ISSN 0926-6690
Éditeur: Elsevier BV
DOI: 10.1016/j.indcrop.2025.122177

Compliant mechanical response of the ultrafast folding protein EnHD under force

Auteurs: Antonio Reifs, Irene Ruiz Ortiz, Amaia Ochandorena Saa, Jörg Schönfelder, David De Sancho, Victor Muñoz, Raul Perez-Jimenez
Publié dans: Communications Physics, Numéro 6, 2023, ISSN 2399-3650
Éditeur: Springer Nature
DOI: 10.1038/s42005-022-01125-5

Evolution of CRISPR-associated endonucleases as inferred from resurrected proteins

Auteurs: Borja Alonso-Lerma, Ylenia Jabalera, Sara Samperio, Matias Morin, Almudena Fernandez, Logan T. Hille, Rachel A. Silverstein, Ane Quesada-Ganuza, Antonio Reifs, Sergio Fernández-Peñalver, Yolanda Benitez, Lucia Soletto, Jose A. Gavira, Adrian Diaz, Wim Vranken, Avencia Sanchez-Mejias, Marc Güell, Francisco J. M. Mojica, Benjamin P. Kleinstiver, Miguel A. Moreno-Pelayo, Lluis Montoliu, Raul Perez
Publié dans: Nature Microbiology, Numéro 8/1, 2023, Page(s) 77–90, ISSN 2058-5276
Éditeur: Nature Publishing Group
DOI: 10.1038/s41564-022-01265-y

Loosenin-Like Proteins from Phanerochaete carnosa Impact Both Cellulose and Chitin Fiber Networks

Auteurs: Mareike Monschein, Eleni Ioannou, Taru Koitto, Leamon A. K. M. Al Amin, Jutta J. Varis, Edward R. Wagner, Kirsi S. Mikkonen, Daniel J. Cosgrove, Emma R. Master
Publié dans: Applied and Environmental Microbiology, Numéro 89/1, 2023, ISSN 0099-2240
Éditeur: American Society for Microbiology
DOI: 10.1128/aem.01863-22

Phylogenetically distinct fungal expansins show different binding preferences towards cellulosic materials and enhance cellulase activity

Auteurs: Taru Koitto, Deepika Dahiya, Martina Huusela, Merja Penttilä, Emma Master
Publié dans: Current Research in Biotechnology, Numéro 9, 2025, Page(s) 100296, ISSN 2590-2628
Éditeur: Elsevier BV
DOI: 10.1016/j.crbiot.2025.100296

Conference proceedings (1)

Microbial Expansin Related Proteins for Improved Enzymatic Action for Conversion of Lignocellulose to Platform Sugars

Auteurs: Deepika Dahiya, Siiri de Ruijter, Kim Kutvonen, Emma Master, Amy Yan Wang
Publié dans: NWBC 2022: The 10th Nordic Wood Biorefinery Conference, 2022, Page(s) 220-221, ISBN 978-951-38-8772-8
Éditeur: VTT Technical Research Centre of Finland
DOI: 10.32040/2242-122x.2022.t409