Description du projet
Un échafaudage innovant pour la culture cellulaire
La médecine régénérative implique la réparation, le remplacement ou la restauration de cellules ou de tissus endommagés. La clé de cette stratégie est la culture efficace de cellules souches ou différenciées in vitro. Pour résoudre les problèmes techniques associés aux cultures cellulaires in vitro, le projet PiezoGel financé par l’UE a développé un nouveau système qui combine un hydrogel supportant les cellules avec des peptides piézoélectriques capables de générer de l’énergie électrique en réponse à une déformation mécanique. Les chercheurs optimiseront la technologie PiezoGel pour la croissance in vitro de cultures organoïdes ainsi que pour la différenciation des cellules souches. Les applications futures incluent la régénération tissulaire et le développement de médicaments.
Objectif
Tissue regeneration has emerged as a promising novel therapy for various disease conditions. A key requirement for the implementation of this advanced approach is the efficient, reliable and reproducible growth of 3D cell cultures, including organoid structures. State-of-the-art 3D culture media support the growth of such cultures, yet exhibit several key setbacks, including low reproducibility and limited modularity. Moreover, no commercial piezoelectric media are currently available, thus prohibiting the option of inducing electrical stimulation of the cells via mechanical stimuli, similar to the in vivo function of several tissues. Here, we aim to develop PiezoGel, a biocompatible, reproducible, controllable and piezoelectric medium for 3D cell cultures. The newly-designed medium will be based on two components, a cell-supporting hydrogel and a piezoelectric self-assembled peptide structure. In the scope of the BISON-694426 Advanced ERC project, we identified promising molecular building blocks for each of these components. The Proof of Concept project will focus both on technological development of the PiezoGel matrix and on business feasibility. Thus, the formulation of the newly-designed cell medium will be optimized, and the resulting matrix will be examined for various properties, including mechanical rigidity and piezoelectricity. The growth of diverse organoid cultures, as well as stem cell differentiation, will be further tested and calibrated. Relevant stakeholders will be approached allowing to map the product requirements and expected features directly from the users. In parallel, the regulatory compliance of the PiezoGel medium will be verified, and the relevant material and methodologies will be patented. We envision diverse applications for the PiezoGel technology, including establishing 3D cell cultures as drug development platforms, basic research exploration, and further advancement of the tissue regeneration field.
Champ scientifique
- medical and health sciencesbasic medicinepharmacology and pharmacydrug discovery
- natural sciencesbiological sciencesbiochemistrybiomolecules
- medical and health sciencesmedical biotechnologycells technologiesstem cells
- engineering and technologyelectrical engineering, electronic engineering, information engineeringelectrical engineeringpiezoelectrics
Mots‑clés
Programme(s)
Régime de financement
ERC-POC - Proof of Concept GrantInstitution d’accueil
69978 Tel Aviv
Israël