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CHARACTERISATION OF TMAO ASE ACTIVITY AND INHIBITON OF FORMATION OF FORMALDEHYDE AND DIMETHYLAMINE IN FISHERY PRODUCTS

Objective



Objectives:

To purify and characterize the TMAOase to get deeper insight into the occurrence, properties and molecular mechanism of this enzyme.

To achieve this objective sophisticated methods for solubility and chromatography will be applied for the purification of the membrane bound TMAOase . Advanced techniques like HPLC, fluorimetry and spectroscopy will be used for identification and characterization of co-factors.

As properties and intracellular localization of TMAOase from various fish species may be considerably different, the enzyme will be purified from two commercially important fish species, saithe and hake.

Funding Scheme

CSC - Cost-sharing contracts

Coordinator

Bundesforschungsanstalt für Fischerei
Address
Palmaille 9
22767 Hamburg
Germany

Participants (2)

CSIC
Spain
Address
Serrano
28006 Vigo
DANMARKS TEKNISKE HOEJSKOLE
Denmark
Address
Technical University
2800 Lyngby