CHARACTERISATION OF TMAO ASE ACTIVITY AND INHIBITON OF FORMATION OF FORMALDEHYDE AND DIMETHYLAMINE IN FISHERY PRODUCTS

Objective

Objectives:

To purify and characterize the TMAOase to get deeper insight into the occurrence, properties and molecular mechanism of this enzyme.

To achieve this objective sophisticated methods for solubility and chromatography will be applied for the purification of the membrane bound TMAOase . Advanced techniques like HPLC, fluorimetry and spectroscopy will be used for identification and characterization of co-factors.

As properties and intracellular localization of TMAOase from various fish species may be considerably different, the enzyme will be purified from two commercially important fish species, saithe and hake.

Programme(s)

• FP3-AIR - Specific research and technological development and demonstration programme (EEC) in the field of agriculture and agro-industry, including fisheries, 1990-1994

Topic(s)

• 311 - Food safety

Funding Scheme

CSC - Cost-sharing contracts

Coordinator

Bundesforschungsanstalt für Fischerei

Address

Palmaille 9
22767 Hamburg
Germany

Participants (2)

CSIC

Spain

Address

Serrano
28006 Vigo

DANMARKS TEKNISKE HOEJSKOLE

Denmark

Address

Technical University
2800 Lyngby