Exploitable results
An assessment is being made on the effect of introduced genetically modified biological control agents on the residual microflora and the control of soil borne fungal plant pathogens. Microcosm trials have been conducted to monitor the colonization and persistence of genetically modified microorganisms (GMM) on the roots of agronomically important crops. In addition, assessment of the effect of GMM inoculants on microbial biomass in the rhizosphere and the level of native beneficial bacteria (eg Rhizobium, Pseudomonas, Azospirillum and Mycorrhiza) has been evaluated. Molecular methods and polymerase chain reaction (PCR) based probe systems have been developed to enable the monitoring of interactions between inoculant and resident strains and to identify and characterize the diversity of specific bacterial populations in the rhizosphere. The rhizosphere of transgenic plants are currently being investigated in glasshouse trials. Transgenic plants, producing chitinase, are being investigated for their effect on introduced biological control agents and the resident microflora. An estimate of the introduced population that is viable but nonculturable (VBNC) has been made. An assessment of whether containment systems in Rhizobium leads to cell death or unculturability is presently being investigated.
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