In vitro selection of RNA structural motifs with self-splicing activity

Informazioni relative al progetto

ID dell’accordo di sovvenzione: BIO2930345

Progetto chiuso

Data di avvio 1 Settembre 1993

Data di completamento 28 Febbraio 1997

Finanziato da

• Specific research and technological development programme (EEC) in the field of biotechnology, 1990-1994

Costo totale

Nessun dato

Contributo UE

€ 550 000,00

Coordinato da

CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE
France

CORDIS fornisce collegamenti ai risultati finali pubblici e alle pubblicazioni dei progetti ORIZZONTE.

I link ai risultati e alle pubblicazioni dei progetti del 7° PQ, così come i link ad alcuni tipi di risultati specifici come dataset e software, sono recuperati dinamicamente da .OpenAIRE .

Risultati sfruttabili

In vitro selection of ribonucleic acid structural motifs

The research relies on in vitro darwinian selection of ribonucleic acid (RNA) molecules to produce RNA structural motifs with designed characteristics (aptamers): self splicing activity, potential for recognition of other RNA motifs, ability to bind specifically antibiotics or amino acids. The structural identification of the motifs is performed using chemical footprinting techniques, modelling, and possibly X-ray analysis. Aptamers have been obtained with affinity for aminoglycoside and peptide antibiotics, with affinity for flavine cofactors, as well as aptamers distinguishing arginine from citrulline (change from an amino to a carbonyl group) or able to differentiate the most common RNA tetraloops. Structural characterization of some of the aptamers is well under way, as well as crystallization experiments.