- The BEG-Net will aim to build up a network of laboratories working on a specific group of symbiotic fungi, in the Glomales (arbuscular mycorrhizal fungi - AMF), which are critical to the growth and development of many plants in natural and agro-ecosystems but which cannot be cultured alone on synthetic medium. The collection of AMF as well as genetic information including gene libraries, isolated clones or sequences of certain genes will be made accessible to the scientific community. The BEG-Net will focus on these non-culturable fungi which will serve as a model for future European, as opposed to National, collections of economically important non-culturable micro-organisms.
- Cultures of AMF will continue to be registered in the BEG (La Banque Européenne des Glomales) database which allocates a serial code number to each pure isolate of AMF (BEG -) and allows information on the isolates to be collated. BEG-Net will switch the database from a stand-alone workstation system to an on-line (Internet) browsable version within the first year.
- Living cultures are also maintained and some of the most frequently used are made available. More isolates will be made available during the lifespan of the project. As a new method for facilitated maintenance, in vitro root organ cultures of AMF will also be built up.
- A demonstration Expert System on CD-ROM has been developed to aid identification, teaching and awareness of these fungi and a second version will be a major aim of the project.
- Molecular methods will be used to build up genomic and cDNA libraries, as well as for the analysis of gene expression on transcript and protein levels and isozyme profiles for target AMF. This new information will be incorporated into the BEG database.
- The links between the BEG-Net and BIORIZE (a small biotechnology company) will be established to pursue the aim of exploiting this natural biotechnological resource. The BEG-Net will advise the company on quality control and provide scientific information useful in predicting where inoculants would be most successful.
- The training and mobility of young scientists will be an important facet of the BEG-Net involving several short inter-laboratory visits by young research scientists.
Main Achievements to date:
- Production of browsable on-line (WWW) database for the BEG in Year 1.
- The new online browsable Genetic Archive is completed and cross-linked to the BEG Database in Year 2.
- Availability of 18 cultures of AMF and PCR primer sequences for the detection and identification of glomalean species.
HIGHLIGHT!
- The collaboration of the BEG with a SME (BIORIZE) to allow screening, testing and production of quality inocula of AMF. The involvement of BIORIZE in the Concerted Action has resulted in an increasing customer base for the company, 4 in 1997, 8 in 1998 and now 22 so far in 1999.
- The advantages of BEGNet for BIORIZE have been both commercial and technical. BIORIZE now has an image as a serious company with a scientific and technical approach in the production of AMF inoculants. The company has also been contacted through the BEG-Net WWW home pages to supply quality-assured AMF inoculum with BEG codes.