Ziel
- This project attempts to exploit the metallo-adsorbent properties of three different microbial-based systems to develop new biomaterials for the engineering of novel and efficacious processes for metal ion removal. The three systems include the minicapsular, galactosamine-containing polymer named Emulsan, the metal-accumulating strains of Alcaligenes eutrophus and the cell surface proteins of Pseudomonas putida. In the three cases, the metal-binding properties of each system will be examined and improved through a combination of biochemical, physiological and genetic approaches and their performance in real situations will be assessed in a variety of environmental conditions.
Results so far
- Amino-acid sequences of two metal binding proteins of A. eutrophus ER121 are determined and indicate at least one Cd-binding protein.
- A Vector system to insert metal binding polymers in outer membrane proteins was constructed.
- Some first Pseudomonas strains with metal binding polymers, integrated in the outer membrane proteins, were constructed.
- The metal bioseroption characteristics of Pseudomoas mendocina A5302 were studied.
- Characterisation of the heavy metal binding capacity and specificity of strains such as Acinetobacter iwolffi 1437, Alcaligenes eutrophus CH34 and ER121 and Pseudomonas mendocina AS302;
- Isolation of metal binding polymers (polysaccharides, proteins) from CH34, ER121 and AS302;
- Cloning of genes encoding metal binding proteins;
- Construction of a vector system to insert metal binding polymers in outer membrane proteins;
- Construction of recombinant Pseudomonas strains with metal binding polymers (metallothioneins, new isolated proteins) integrated in the outer membrane proteins;
- Comparison of metal recovery by the different biomasses in three reactor types such as packed bed column, membrane bioreactor, chemostat, etc.
Programm/Programme
Thema/Themen
Data not availableAufforderung zur Vorschlagseinreichung
Data not availableFinanzierungsplan
CSC - Cost-sharing contractsKoordinator
2400 Mol
Belgien