CORDIS proporciona enlaces a los documentos públicos y las publicaciones de los proyectos de los programas marco HORIZONTE.
Los enlaces a los documentos y las publicaciones de los proyectos del Séptimo Programa Marco, así como los enlaces a algunos tipos de resultados específicos, como conjuntos de datos y «software», se obtienen dinámicamente de OpenAIRE .
Resultados aprovechables
A disposable sensor for the detection of polychlorinated biphenyls (PCB) in soil samples has been developed using polyclonal antibodies as recognizing element and carbon-based screen printed electrochemical electrodes as sensing element. The analysis scheme has been on the competitive enzyme linked immunosorbent assay (ELISA) protocol. The immunochemical reaction has been carried on using an Immobilon membrane as the solid phase. The enzyme used for the measurement of the immunoreaction has been alkaline phosphatase whereas the electrochemical substate was alpha-naphthyl phosphate. The extent of the immunochemical reaction has been monitored by means of an electrochemical product using screen printed electrodes and differential pulse voltammentry (DPV). The screen printed electrodes were characterized and their electrochemical performance tested. Soil samples were assayed using a simple extraction kit. A detection limit of 0.3 - 0.6 ng/ml in solution was achieved with the proposed methods regardless of PCB mixture standard.
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