Monitoring of polychlorinated biphenyls (PCBs) in ground waters through an immunochemical biosensor with electrochemical detection

Informations projet

N° de convention de subvention: EV5V0407

Projet clôturé

Date de début 1 Mai 1994

Date de fin 30 Avril 1997

Financé au titre de

• Specific research and technological development programme (EEC) in the field of the environment, 1990-1994

Coût total

Aucune donnée

Contribution de l’UE

Aucune donnée

Coordonné par

UNIVERSITY OF FLORENCE
Italy

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Résultats exploitables

Monitoring of polychlorinated biphenyls (PCBs) in ground waters through an immunochemical biosensor with electrochemical detection

A disposable sensor for the detection of polychlorinated biphenyls (PCB) in soil samples has been developed using polyclonal antibodies as recognizing element and carbon-based screen printed electrochemical electrodes as sensing element. The analysis scheme has been on the competitive enzyme linked immunosorbent assay (ELISA) protocol. The immunochemical reaction has been carried on using an Immobilon membrane as the solid phase. The enzyme used for the measurement of the immunoreaction has been alkaline phosphatase whereas the electrochemical substate was alpha-naphthyl phosphate. The extent of the immunochemical reaction has been monitored by means of an electrochemical product using screen printed electrodes and differential pulse voltammentry (DPV). The screen printed electrodes were characterized and their electrochemical performance tested. Soil samples were assayed using a simple extraction kit. A detection limit of 0.3 - 0.6 ng/ml in solution was achieved with the proposed methods regardless of PCB mixture standard.