Monitoring of polychlorinated biphenyls (PCBs) in ground waters through an immunochemical biosensor with electrochemical detection

Projektinformationen

ID Finanzhilfevereinbarung: EV5V0407

Projekt abgeschlossen

Startdatum 1 Mai 1994

Enddatum 30 April 1997

Finanziert unter

• Specific research and technological development programme (EEC) in the field of the environment, 1990-1994

Gesamtkosten

Keine Daten

EU-Beitrag

Keine Daten

Koordiniert durch

UNIVERSITY OF FLORENCE
Italy

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Verwertbare Ergebnisse

Monitoring of polychlorinated biphenyls (PCBs) in ground waters through an immunochemical biosensor with electrochemical detection

A disposable sensor for the detection of polychlorinated biphenyls (PCB) in soil samples has been developed using polyclonal antibodies as recognizing element and carbon-based screen printed electrochemical electrodes as sensing element. The analysis scheme has been on the competitive enzyme linked immunosorbent assay (ELISA) protocol. The immunochemical reaction has been carried on using an Immobilon membrane as the solid phase. The enzyme used for the measurement of the immunoreaction has been alkaline phosphatase whereas the electrochemical substate was alpha-naphthyl phosphate. The extent of the immunochemical reaction has been monitored by means of an electrochemical product using screen printed electrodes and differential pulse voltammentry (DPV). The screen printed electrodes were characterized and their electrochemical performance tested. Soil samples were assayed using a simple extraction kit. A detection limit of 0.3 - 0.6 ng/ml in solution was achieved with the proposed methods regardless of PCB mixture standard.