Objectif Scientific objectives and approach:The objective of this project is to develop an automated sensor that enables a quick, on-site assessment of genotoxicity to overcome the drawbacks of the current "comet assay". It will be based on the single cell gel (SCG) electrophoresis method, that is the so-called "comet assay", which is the best current technique for genotoxicity assessment.The innovation of this development is based on completely new equipment composed of:- The "multi-chamber wells" that is new tool enabling the on-site collection of samples and preventing the manipulation of probes during the test- An "automated carrier" that will support the multi-chamber wells and moves it under the microscope- A powerful software controlling the tray of the wells and interface to existing analysis software that will also be developed in the project.This development will enable the measure of up to 200-400 probes per day, instead of assessing 12-24 probes/1,5 day with the best current technique.Problems to be solved:Determination of the genotoxic potential of man-made chemicals (i.e. effluents, biozides, and chemicals accidentally released into the environment) is a subject of investigation all over the world. Methods measuring genotoxicity are time and cost consuming and need well-equipped cell culture laboratories as well as technical / scientific staff with experience in cell culture techniques.During the last 10 years, the comet assay (also named single cell gel electrophoresis assay (SCGE)) has been developed to a very sensitive assay for testing genotoxic properties of chemical and physical toxicants in mammalian cells.The disadvantages of the comet assay, as it is the case in all other modern genotoxicity tests lies in the small number of samples, which can be examined within one day. Highly specialized staff and a well-equipped cell culture laboratory are necessary to perform the assay.Expected Impacts:The impacts are:- increase of the number of samples/person and day- Reduction of required measuring time by 75% compared to existing methods (approximately 1 hour for 25 probes, instead of 12 probes a day)- Reduction of measuring costs by 50% compared to existing laboratory methods (automation of the sensor, then no need of high skilled trained personal in small companies to perform the test)- simplification of the procedure- enabling non-specialists to perform the assay- cell culture laboratory not necessary- greater competitiveness and profitability for the procedure of chemicals, biozides, effluents, by better control of genotoxicity. Champ scientifique natural sciencescomputer and information sciencessoftwarenatural sciencesphysical sciencesopticsmicroscopynatural sciencesphysical sciencesastronomyplanetary sciencescometsengineering and technologyelectrical engineering, electronic engineering, information engineeringelectronic engineeringsensorsnatural scienceschemical scienceselectrochemistryelectrophoresis Programme(s) FP5-EESD - Programme for research, technological development and demonstration on "Energy, environment and sustainable development, 1998-2002" Thème(s) 1.1.4.-1. - Key action Sustainable Management and Quality of Water Appel à propositions Data not available Régime de financement CRS - Cooperative research contracts Coordinateur INTOX GMBH Contribution de l’UE Aucune donnée Adresse An den Eschen 43B 26129 OLDENBURG Allemagne Voir sur la carte Coût total Aucune donnée Participants (8) Trier par ordre alphabétique Trier par contribution de l’UE Tout développer Tout réduire EGI- AMBIENTE, PRESTACAO DE GESTAO E CONTROLE AMBIENTAL LDA Portugal Contribution de l’UE Aucune donnée Adresse Praceta Madre de Deus 44 4405 VALANDARES Voir sur la carte Coût total Aucune donnée INSTITUTO TECNOLOGICO DE CASTILLA Y LEON Espagne Contribution de l’UE Aucune donnée Adresse C/ Lopez Bravo, 70 - Pol. Ind. Villalonquejar 09001 BURGOS Voir sur la carte Coût total Aucune donnée ISMAR CHIMICA SPA Italie Contribution de l’UE Aucune donnée Adresse Via Isocorte 16 16164 GENOVA Voir sur la carte Coût total Aucune donnée ISTITUTO NAZIONALE PER LO STUDIO E LA CURA DEI TUMORI Italie Contribution de l’UE Aucune donnée Adresse Via Mariano Semmola 80131 NAPOLI Voir sur la carte Coût total Aucune donnée MESUREX S.L. Espagne Contribution de l’UE Aucune donnée Adresse Pje. Pandereta, local 1 FUENGIROLA Voir sur la carte Liens Site web Opens in new window Coût total Aucune donnée PARALAB-EQUIPAMENTOS INDUSTRIAIS E DE LABORATORIO, S.A. Portugal Contribution de l’UE Aucune donnée Adresse Pç. Dos Pescadores 47 4450 333 MATOSINHOS Voir sur la carte Coût total Aucune donnée SOCIETA TECNOLOGIE BIOMEDICHE SRL Italie Contribution de l’UE Aucune donnée Adresse Viale delle acacie, 44 Edf A/5A 81031 AVERSA Voir sur la carte Coût total Aucune donnée UNIVERSITY OF OLDENBURG Allemagne Contribution de l’UE Aucune donnée Adresse Ammerlaender Heerstrasse 114/118 26111 OLDENBURG Voir sur la carte Coût total Aucune donnée